FastQCFastQC Report
Fri 17 Jun 2016
SRR1527111_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527111_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1483107
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC143870.9700581279705375No Hit
TGTCACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC113870.7677800725099403No Hit
TGTCACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG72060.4858718892163546No Hit
TGTCACGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCA40760.2748284513524648No Hit
TGTCACAGGAGTCTAGAACACACAAAACTCGCAGGCAATCCCGGGGCCAG34060.22965301896626472No Hit
TGTCACGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA33820.22803479452258No Hit
TGTCACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT32150.21677464943527341No Hit
TGTCACGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG31790.21434731276974622No Hit
TGTCACGGGGACTGTCGAGTCGCGTCCACCCGCGAGCACAGCTTCTTTGC31680.2136056265663907No Hit
TGTCACGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG30150.20328944573790023No Hit
TGTCACGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT29330.19776051222197724No Hit
TGTCACGGGGACAGCATGCTCTCTCCCTATAGGCACGGCCTCCGCCGTGT26380.17786983676835183No Hit
TGTCACGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTT25320.1707226788087441No Hit
TGTCACGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC24330.16404750297854437No Hit
TGTCACGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTC23380.15764203122229212No Hit
TGTCACGGGGAAGTGTTCCTGGTCTGTGTGTGTGGCTGTCCGTGTTGTCG23270.1569003450189366No Hit
TGTCACGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAA22520.15184339363242166No Hit
TGTCACGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC20610.1389650241014303No Hit
TGTCACGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT20300.13687481752833747No Hit
TGTCACGGCACGACGCTACTCAGACTACCCAGATGCTTACACCACATGAA20080.13539144512162643No Hit
TGTCACGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATC20010.13491946299221835No Hit
TGTCACGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC17850.12035544299905537No Hit
TGTCACGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCT17780.1198834608696473No Hit
TGTCACGGGGAGACGTTCTCGCCCAGAGTCGCCGCGGTTTCCTGCTTCAA16080.10842103772688012No Hit
TGTCACGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCT16000.1078816295789852No Hit
TGTCACGGGACCTTCAAGGCTTTTGACAAGCATATGAATTTGATCCTCTG15490.10444290263615505No Hit
TGTCACGGGGGGGCCTCCTCAACTTTCCTGATGACTCTACCCATAGCAGG15110.10188071393365415No Hit
TGTCACGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCT14940.10073447161937743No Hit
TGTCACGGGACTTTCAGAGTCCATAGAATGTTGACACAGTGATTCTTTGA14940.10073447161937743No Hit
TGTCACGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACA14860.1001950634714825No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCATT408.314601E-944.00000425
CCCTCGA207.8572216E-444.00000428
CGTATAA207.8572216E-444.00000423
CCGATTA207.8572216E-444.00000444
GTTTACG207.8572216E-444.00000433
TTTAACG207.8572216E-444.00000441
CGTGATA207.8572216E-444.00000432
CGACAGT207.8572216E-444.00000439
CTACGTC207.8572216E-444.00000416
TTGCGTA207.8572216E-444.00000442
GCGATAA207.8572216E-444.00000436
ACGTCGT207.8572216E-444.00000418
TTACGAA207.8572216E-444.00000435
TCATATA650.044.00000431
TGTACGA207.8572216E-444.00000420
CGATCAC207.8572216E-444.00000421
CGATACC207.8572216E-444.00000413
GTAAATC207.8572216E-444.00000423
AATCGGT207.8572216E-444.00000435
TACGTCG207.8572216E-444.00000417
Produced by FastQC (version 0.11.5)