##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527111_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1483107
Sequences flagged as poor quality	0
Sequence length	50
%GC	57
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.42774324441864	33.0	31.0	34.0	31.0	34.0
2	32.72555587695291	34.0	31.0	34.0	31.0	34.0
3	31.858859138282	31.0	31.0	34.0	30.0	34.0
4	36.2137033942932	37.0	35.0	37.0	35.0	37.0
5	36.13784440367418	37.0	35.0	37.0	35.0	37.0
6	36.311227713172414	37.0	37.0	37.0	35.0	37.0
7	36.4618918257415	37.0	37.0	37.0	35.0	37.0
8	36.62941311719249	37.0	37.0	37.0	35.0	37.0
9	38.73688277379852	39.0	39.0	39.0	39.0	39.0
10	38.23019782119564	39.0	39.0	39.0	37.0	39.0
11	37.82480023356373	39.0	38.0	39.0	35.0	39.0
12	37.0635476739035	39.0	35.0	39.0	34.0	39.0
13	36.83322444031348	39.0	35.0	39.0	33.0	39.0
14	37.49538098060356	40.0	37.0	40.0	33.0	40.0
15	37.54802451879736	40.0	36.0	40.0	33.0	40.0
16	37.46824537946352	40.0	36.0	40.0	33.0	40.0
17	37.4338452990917	40.0	36.0	40.0	33.0	40.0
18	37.37459266256582	40.0	36.0	40.0	33.0	40.0
19	37.31679170821795	40.0	36.0	40.0	32.0	40.0
20	36.955250700050634	39.0	35.0	40.0	31.0	40.0
21	37.228024006359625	39.0	36.0	40.0	32.0	40.0
22	37.10377538505313	39.0	35.0	40.0	32.0	40.0
23	37.16305094642531	39.0	35.0	40.0	32.0	40.0
24	37.19037466615693	39.0	35.0	40.0	33.0	40.0
25	37.16931549780292	39.0	35.0	40.0	33.0	40.0
26	36.99312928871619	39.0	35.0	40.0	32.0	40.0
27	36.841320282353195	39.0	35.0	40.0	32.0	40.0
28	36.67342207945887	38.0	35.0	40.0	31.0	40.0
29	36.9425038112557	39.0	35.0	40.0	32.0	40.0
30	37.03384853554059	39.0	35.0	40.0	33.0	40.0
31	37.01711609479289	39.0	35.0	40.0	33.0	40.0
32	36.33686510818168	39.0	35.0	40.0	31.0	40.0
33	36.477177304132475	39.0	35.0	40.0	31.0	40.0
34	36.401560372919825	39.0	35.0	40.0	31.0	40.0
35	36.32679907788177	39.0	35.0	40.0	31.0	40.0
36	36.18103953389742	39.0	35.0	40.0	31.0	40.0
37	36.07843601304559	38.0	35.0	40.0	30.0	40.0
38	35.842300656662	38.0	35.0	40.0	30.0	40.0
39	35.78285585598342	38.0	35.0	40.0	30.0	40.0
40	35.59722528448723	37.0	35.0	40.0	29.0	40.0
41	35.44482427768192	37.0	34.0	40.0	29.0	40.0
42	35.3857469488041	37.0	34.0	40.0	29.0	40.0
43	35.28841277129701	37.0	34.0	40.0	29.0	40.0
44	35.09290698513324	36.0	34.0	40.0	28.0	40.0
45	34.97450554814993	36.0	34.0	40.0	28.0	40.0
46	34.71704334211894	36.0	34.0	40.0	27.0	40.0
47	34.5838762813472	35.0	34.0	40.0	26.0	40.0
48	34.48948659806744	35.0	34.0	40.0	27.0	40.0
49	34.21109265885738	35.0	33.0	39.0	26.0	40.0
50	33.72195667608608	35.0	33.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	4.0
13	4.0
14	12.0
15	30.0
16	69.0
17	140.0
18	302.0
19	583.0
20	935.0
21	1594.0
22	2588.0
23	3821.0
24	5363.0
25	7056.0
26	9654.0
27	12963.0
28	17692.0
29	23366.0
30	31210.0
31	38550.0
32	46916.0
33	66416.0
34	127478.0
35	124513.0
36	158146.0
37	246722.0
38	407149.0
39	149831.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	100.0	0.0	0.0	0.0
3	0.0	0.0	100.0	0.0
4	0.0	0.0	0.0	100.0
5	0.0	100.0	0.0	0.0
6	0.0	0.0	0.0	100.0
7	99.15825358520996	0.6955668067105071	0.04928841951389886	0.09689118856562608
8	99.52012902642898	0.3084740345774108	0.07072989339272216	0.10066704560089057
9	98.09191110284019	0.9114649179054511	0.37279845621388075	0.6238255230404819
10	63.50782512657549	25.122395080058286	4.244400437729712	7.125379355636512
11	39.02503325788362	23.23257863390841	18.60735604376488	19.13503206444309
12	32.726836297043974	14.87060609922278	26.63334472833046	25.769212875402786
13	26.179635049932337	15.084346577826144	29.990486188791504	28.745532183450013
14	25.09171624164676	15.522683124009259	33.187760559420184	26.19784007492379
15	27.17875379187071	17.91630678029299	29.861432789407644	25.043506638428646
16	32.85258582152198	18.895804550851693	24.430671556401528	23.820938071224802
17	32.47614636030981	19.465014998917813	23.592363868554326	24.466474772218053
18	31.39126172285614	19.80410044588826	23.156791789129173	25.647846042126428
19	31.35646989731692	21.399602321342964	21.682859025006287	25.56106875633383
20	29.838845073214543	22.81136829642096	24.07715694147489	23.27262968888961
21	30.5849813937902	19.874156079096114	24.560534068007232	24.980328459106456
22	29.902495234666144	20.064904285395457	26.218000454451364	23.814600025487035
23	28.42465176147102	20.836999623088555	25.465998070267347	25.272350545173072
24	30.55329116510137	20.740310712578392	22.853037575845843	25.853360546474395
25	28.5248468249425	21.38234126061033	23.256582296489732	26.836229617957436
26	26.531396588378314	20.472359715111587	23.296970481563367	29.699273214946732
27	25.85706897749117	18.176166655541373	25.451973458422085	30.51479090854537
28	26.85504147711527	21.24000493558455	22.99577845698254	28.90917513031764
29	28.987793867873325	21.470871622883582	22.85694828491808	26.684386224325014
30	28.751263395021397	18.76007597563763	24.58399832244066	27.904662306900313
31	29.606090457397883	19.986690103950693	25.073915772766224	25.333303665885197
32	34.27979235483347	19.840105939760246	21.42401054003521	24.456091165371074
33	33.15141793545577	19.92634381740495	23.10183958406238	23.820398663076904
34	29.516481278828838	21.26110927937094	26.94869621679353	22.27371322500669
35	27.30389648218234	26.437674422681575	24.811224004741398	21.447205090394693
36	28.79252811833536	26.511843043017123	22.242629830484248	22.452999008163268
37	29.47771131819889	26.664158418778953	21.039412530586127	22.818717732436028
38	31.208672064793706	25.83839197037031	19.858378390770188	23.094557574065796
39	30.633460701082253	22.564117086629622	21.191525628292496	25.610896583995626
40	29.292087489304546	21.458060679371076	21.22968875475606	28.02016307656831
41	24.32137398043432	23.459602038153687	22.685281641850523	29.533742339561474
42	24.282941149896807	21.879203590840042	23.2095189355859	30.628336323677253
43	26.0586727727669	20.243987790496572	24.99725238974666	28.700087046989864
44	27.333226800224125	19.38632883534364	24.25273429361469	29.027710070817548
45	26.91761282227108	20.062342096692955	23.2203070985438	29.799737982492157
46	26.691735660340083	22.3419483557154	22.581715277454695	28.38460070648982
47	25.556686065132183	22.425556618639114	25.455209907309452	26.562547408919247
48	27.343542981052614	22.744818816174423	24.530596915799062	25.3810412869739
49	29.230460108407552	21.18694065903539	23.675837279441065	25.906761953115993
50	24.852421302036873	21.939347599330326	24.381383136887628	28.826847961745173
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	1.0
6	2.0
7	2.0
8	2.0
9	44.5
10	87.0
11	190.5
12	294.0
13	1642.0
14	2990.0
15	3741.0
16	4492.0
17	4140.0
18	3788.0
19	3488.0
20	3188.0
21	3397.5
22	3607.0
23	3580.5
24	3554.0
25	3892.5
26	4231.0
27	4683.0
28	5135.0
29	6941.5
30	8748.0
31	9095.5
32	9443.0
33	10181.0
34	10919.0
35	14082.5
36	17246.0
37	19423.0
38	21600.0
39	23420.5
40	25241.0
41	26904.0
42	28567.0
43	32553.0
44	36539.0
45	36705.0
46	36871.0
47	43131.5
48	49392.0
49	50698.0
50	52004.0
51	60243.0
52	68482.0
53	74051.5
54	79621.0
55	90172.0
56	100723.0
57	117748.0
58	134773.0
59	142201.5
60	149630.0
61	153117.5
62	156605.0
63	151316.0
64	146027.0
65	127448.0
66	108869.0
67	88947.5
68	69026.0
69	58774.5
70	48523.0
71	43224.0
72	37925.0
73	31282.5
74	24640.0
75	21644.0
76	18648.0
77	13064.5
78	7481.0
79	4907.0
80	2333.0
81	1804.0
82	1275.0
83	877.0
84	479.0
85	272.5
86	66.0
87	47.0
88	28.0
89	20.0
90	12.0
91	6.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1483107.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.13735096434957
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.19703917582784	5.549432425032082
2	11.467053968782452	1.8662288534224092
3	4.848247856032644	1.1835568310007893
4	2.543063869961416	0.8277521293853232
5	1.475974806101849	0.6005262505894275
6	0.8390058325616633	0.40963709524143394
7	0.5751815134123275	0.3276317689989294
8	0.4160646328513401	0.27085311530916817
9	0.2807789586469941	0.20563172369236665
>10	3.347222859819219	6.987457272169023
>50	2.5082899941470274	15.196033570954986
>100	3.3131890221195808	49.577283677484736
>500	0.1301792296826646	7.0825185512223845
>1k	0.05615574613762001	7.632046384851127
>5k	8.508446384487881E-4	0.4989161609082409
>10k+	0.0017016892768975762	1.7844941897375801
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TGTCACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	14387	0.9700581279705375	No Hit
TGTCACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	11387	0.7677800725099403	No Hit
TGTCACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	7206	0.4858718892163546	No Hit
TGTCACGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCA	4076	0.2748284513524648	No Hit
TGTCACAGGAGTCTAGAACACACAAAACTCGCAGGCAATCCCGGGGCCAG	3406	0.22965301896626472	No Hit
TGTCACGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	3382	0.22803479452258	No Hit
TGTCACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	3215	0.21677464943527341	No Hit
TGTCACGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG	3179	0.21434731276974622	No Hit
TGTCACGGGGACTGTCGAGTCGCGTCCACCCGCGAGCACAGCTTCTTTGC	3168	0.2136056265663907	No Hit
TGTCACGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG	3015	0.20328944573790023	No Hit
TGTCACGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	2933	0.19776051222197724	No Hit
TGTCACGGGGACAGCATGCTCTCTCCCTATAGGCACGGCCTCCGCCGTGT	2638	0.17786983676835183	No Hit
TGTCACGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTT	2532	0.1707226788087441	No Hit
TGTCACGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	2433	0.16404750297854437	No Hit
TGTCACGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTC	2338	0.15764203122229212	No Hit
TGTCACGGGGAAGTGTTCCTGGTCTGTGTGTGTGGCTGTCCGTGTTGTCG	2327	0.1569003450189366	No Hit
TGTCACGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAA	2252	0.15184339363242166	No Hit
TGTCACGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC	2061	0.1389650241014303	No Hit
TGTCACGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	2030	0.13687481752833747	No Hit
TGTCACGGCACGACGCTACTCAGACTACCCAGATGCTTACACCACATGAA	2008	0.13539144512162643	No Hit
TGTCACGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATC	2001	0.13491946299221835	No Hit
TGTCACGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	1785	0.12035544299905537	No Hit
TGTCACGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCT	1778	0.1198834608696473	No Hit
TGTCACGGGGAGACGTTCTCGCCCAGAGTCGCCGCGGTTTCCTGCTTCAA	1608	0.10842103772688012	No Hit
TGTCACGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCT	1600	0.1078816295789852	No Hit
TGTCACGGGACCTTCAAGGCTTTTGACAAGCATATGAATTTGATCCTCTG	1549	0.10444290263615505	No Hit
TGTCACGGGGGGGCCTCCTCAACTTTCCTGATGACTCTACCCATAGCAGG	1511	0.10188071393365415	No Hit
TGTCACGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCT	1494	0.10073447161937743	No Hit
TGTCACGGGACTTTCAGAGTCCATAGAATGTTGACACAGTGATTCTTTGA	1494	0.10073447161937743	No Hit
TGTCACGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACA	1486	0.1001950634714825	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	2.69704073947463E-4	0.0	0.0	0.0	0.0
8	4.045561109211945E-4	0.0	0.0	0.0	0.0
9	6.068341663817917E-4	0.0	0.0	0.0	0.0
10	0.003708431016777616	0.0	0.0	0.0	0.0
11	0.00539408147894926	0.0	0.0	0.0	0.0
12	0.007349436015068367	0.0	0.0	0.0	0.0
13	0.011664701198227774	0.0	0.0	0.0	0.0
14	0.017530764806585095	0.0	0.0	0.0	0.0
15	0.023127124340994953	0.0	0.0	0.0	0.0
16	0.03324102711402482	0.0	0.0	0.0	0.0
17	0.038904812666921536	0.0	0.0	0.0	0.0
18	0.04436632016435766	0.0	0.0	0.0	0.0
19	0.05265972043824215	0.0	0.0	0.0	0.0
20	0.057851523861730814	0.0	0.0	0.0	0.0
21	0.0639872915440356	0.0	0.0	0.0	0.0
22	0.07019048524482724	0.0	0.0	0.0	0.0
23	0.07673080903805322	0.0	0.0	0.0	0.0
24	0.08266429866489741	0.0	0.0	0.0	0.0
25	0.0885977882917416	0.0	0.0	0.0	0.0
26	0.09412672180766458	0.0	0.0	0.0	0.0
27	0.09898139513871893	0.0	0.0	0.0	0.0
28	0.10504973680253683	0.0	0.0	0.0	0.0
29	0.10976955809661744	0.0	0.0	0.0	0.0
30	0.1149613615201061	0.0	0.0	0.0	1.348520369737315E-4
31	0.11995088688813417	0.0	0.0	0.0	1.348520369737315E-4
32	0.12750260095866314	0.0	0.0	0.0	1.348520369737315E-4
33	0.1326944043821518	0.0	0.0	0.0	1.348520369737315E-4
34	0.13815591187958792	0.0	0.0	0.0	1.348520369737315E-4
35	0.14698872030136734	0.0	0.0	0.0	1.348520369737315E-4
36	0.16054135001722736	0.0	0.0	0.0	1.348520369737315E-4
37	0.16903702834657244	0.0	0.0	0.0	1.348520369737315E-4
38	0.17692587250953573	0.0	0.0	0.0	1.348520369737315E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCATT	40	8.314601E-9	44.000004	25
CCCTCGA	20	7.8572216E-4	44.000004	28
CGTATAA	20	7.8572216E-4	44.000004	23
CCGATTA	20	7.8572216E-4	44.000004	44
GTTTACG	20	7.8572216E-4	44.000004	33
TTTAACG	20	7.8572216E-4	44.000004	41
CGTGATA	20	7.8572216E-4	44.000004	32
CGACAGT	20	7.8572216E-4	44.000004	39
CTACGTC	20	7.8572216E-4	44.000004	16
TTGCGTA	20	7.8572216E-4	44.000004	42
GCGATAA	20	7.8572216E-4	44.000004	36
ACGTCGT	20	7.8572216E-4	44.000004	18
TTACGAA	20	7.8572216E-4	44.000004	35
TCATATA	65	0.0	44.000004	31
TGTACGA	20	7.8572216E-4	44.000004	20
CGATCAC	20	7.8572216E-4	44.000004	21
CGATACC	20	7.8572216E-4	44.000004	13
GTAAATC	20	7.8572216E-4	44.000004	23
AATCGGT	20	7.8572216E-4	44.000004	35
TACGTCG	20	7.8572216E-4	44.000004	17
>>END_MODULE