FastQCFastQC Report
Fri 17 Jun 2016
SRR1527110_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527110_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1640594
Sequences flagged as poor quality0
Sequence length52
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC146090.890470158979004No Hit
TGTCACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA122770.7483265207601637No Hit
TGTCACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT69880.4259432863950496No Hit
TGTCACGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG43120.26283163293294987No Hit
TGTCACAGGAGTCTAGAACACACAAAACTCGCAGGCAATCCCGGGGCCAGCC34460.21004587362869787No Hit
TGTCACGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC34410.20974110596527845No Hit
TGTCACGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC30200.18407966870535916No Hit
TGTCACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC29770.18145866679995173No Hit
TGTCACGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG29380.17908147902527988No Hit
TGTCACGGGGACTGTCGAGTCGCGTCCACCCGCGAGCACAGCTTCTTTGCAG28760.17530235999887847No Hit
TGTCACGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT28090.17121847330905757No Hit
TGTCACGGGGACAGCATGCTCTCTCCCTATAGGCACGGCCTCCGCCGTGTGG26380.16079541922011173No Hit
TGTCACGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC25090.15293241350388945No Hit
TGTCACGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC24080.14677610670281618No Hit
TGTCACGGGGAAGTGTTCCTGGTCTGTGTGTGTGGCTGTCCGTGTTGTCGCT21450.1307453276069521No Hit
TGTCACGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACG21010.1280633721688608No Hit
TGTCACGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG19710.1201394129199546No Hit
TGTCACGGCACGACGCTACTCAGACTACCCAGATGCTTACACCACATGAAAC19710.1201394129199546No Hit
TGTCACGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG19570.11928606346238008No Hit
TGTCACGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG19490.11879843520090894No Hit
TGTCACGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTT19380.1181279463413861No Hit
TGTCACGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT16410.10002474713426965No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTAT1450.046.00000442
CGTATAC1400.046.00000444
ATTACGT405.6134013E-946.00000424
CAATTGG206.312246E-446.00000440
CGATTAG1500.046.00000430
TATGCGT351.01992555E-746.00000415
TAGTTCG206.312246E-446.00000429
TACGCTA206.312246E-446.00000434
CCCGTAA351.01992555E-746.00000441
CGTAGTA206.312246E-446.00000412
GCGATAA351.01992555E-746.00000436
ATACGCA206.312246E-446.00000414
TATCGTT206.312246E-446.00000421
CGTTTAT206.312246E-446.00000415
CCGTTCA405.6134013E-946.00000433
GTTATCG351.01992555E-746.00000429
GTACGTT351.01992555E-746.00000419
TACGTAT206.312246E-446.00000428
GTACGAA206.312246E-446.00000421
ACGGTAC351.01992555E-746.0000045
Produced by FastQC (version 0.11.5)