##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527110_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1640594
Sequences flagged as poor quality	0
Sequence length	52
%GC	57
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.3405607968821	33.0	31.0	34.0	31.0	34.0
2	32.62145600922592	34.0	31.0	34.0	31.0	34.0
3	31.715125741042574	31.0	31.0	34.0	30.0	34.0
4	36.08106880800491	37.0	35.0	37.0	35.0	37.0
5	36.05719574739393	37.0	35.0	37.0	35.0	37.0
6	36.27682473543119	37.0	37.0	37.0	35.0	37.0
7	36.32708092312906	37.0	37.0	37.0	35.0	37.0
8	36.551263140057806	37.0	37.0	37.0	35.0	37.0
9	38.67525664484936	39.0	39.0	39.0	38.0	39.0
10	38.0857402867498	39.0	38.0	39.0	37.0	39.0
11	37.72284062967437	39.0	38.0	39.0	35.0	39.0
12	37.01179450857433	39.0	35.0	39.0	34.0	39.0
13	36.8352895353756	39.0	35.0	39.0	33.0	39.0
14	37.49929781530348	40.0	36.0	40.0	33.0	40.0
15	37.47072584685791	40.0	36.0	40.0	33.0	40.0
16	32.55043661015461	39.0	33.0	40.0	3.0	40.0
17	34.078587389689346	39.0	33.0	40.0	17.0	40.0
18	36.048746978228614	39.0	34.0	40.0	30.0	40.0
19	36.96181017363223	39.0	35.0	40.0	32.0	40.0
20	37.148799763987924	39.0	35.0	40.0	32.0	40.0
21	37.39566279042835	40.0	36.0	40.0	33.0	40.0
22	37.45149500729614	40.0	36.0	40.0	33.0	40.0
23	37.41829361804322	40.0	35.0	40.0	33.0	40.0
24	37.40397563321577	40.0	35.0	40.0	33.0	40.0
25	37.34272220915108	40.0	35.0	40.0	33.0	40.0
26	37.22863426295598	39.0	35.0	40.0	33.0	40.0
27	37.068879320538784	39.0	35.0	40.0	33.0	40.0
28	36.95548502554563	39.0	35.0	40.0	32.0	40.0
29	36.71174221044329	39.0	35.0	40.0	32.0	40.0
30	36.683711509367946	39.0	35.0	40.0	31.0	40.0
31	36.630410692712516	39.0	35.0	40.0	31.0	40.0
32	36.56610166805437	38.0	35.0	40.0	31.0	40.0
33	36.1853932173347	38.0	35.0	40.0	31.0	40.0
34	35.98099895525645	38.0	35.0	40.0	30.0	40.0
35	35.858531726923296	38.0	34.0	40.0	30.0	40.0
36	35.86214078559351	38.0	34.0	40.0	30.0	40.0
37	35.58329056427123	38.0	34.0	40.0	29.0	40.0
38	35.53937293443716	37.0	34.0	40.0	29.0	40.0
39	35.142767192858194	37.0	34.0	40.0	28.0	40.0
40	35.00979645177296	37.0	34.0	40.0	27.0	40.0
41	34.94396054112108	36.0	34.0	40.0	28.0	40.0
42	34.92620843426222	36.0	34.0	40.0	28.0	40.0
43	34.80578436834464	36.0	33.0	40.0	28.0	40.0
44	35.1431670480326	36.0	34.0	40.0	29.0	40.0
45	35.23832526511739	36.0	34.0	40.0	30.0	40.0
46	35.20142887271317	36.0	34.0	40.0	30.0	40.0
47	34.90655640578961	35.0	34.0	40.0	29.0	40.0
48	34.87010558370932	35.0	34.0	40.0	29.0	40.0
49	34.787885973007334	35.0	34.0	39.0	29.0	40.0
50	34.29056549030412	35.0	34.0	39.0	27.0	40.0
51	34.38097969394012	35.0	34.0	39.0	28.0	40.0
52	34.01517560103231	35.0	33.0	38.0	27.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	4.0
13	1.0
14	7.0
15	29.0
16	78.0
17	181.0
18	356.0
19	633.0
20	1103.0
21	1798.0
22	2823.0
23	4537.0
24	6298.0
25	8848.0
26	11834.0
27	15595.0
28	20939.0
29	27463.0
30	35058.0
31	47297.0
32	63943.0
33	93625.0
34	144118.0
35	163807.0
36	207891.0
37	301120.0
38	354682.0
39	126525.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	100.0	0.0	0.0	0.0
3	0.0	0.0	100.0	0.0
4	0.0	0.0	0.0	100.0
5	0.0	100.0	0.0	0.0
6	0.0	0.0	0.0	100.0
7	99.16188892559646	0.7035256742375018	0.043033194074829	0.09155220609120843
8	99.53650933747167	0.3000132878701251	0.0704622837825812	0.09301509087562188
9	98.14829263059599	0.8709650285201579	0.3292100300257102	0.6515323108581405
10	63.43312239347456	25.30479814018581	4.179522782601911	7.082556683737719
11	39.24743111336504	23.090295344247266	18.54675806445714	19.115515477930554
12	32.9774459738363	14.66432280015653	26.56147712352965	25.796754102477514
13	26.332901375965044	14.985608870933333	29.79256293757017	28.888926815531445
14	25.113891675819854	15.417708464129456	32.9782383697612	26.490161490289495
15	27.038499470313802	18.153242057449923	29.924100661102017	24.884157811134262
16	30.970916631415207	26.812971399383397	21.45856927429943	20.757542694901968
17	32.47567649278249	19.385417720654836	23.600476412811457	24.538429373751214
18	31.414963117017376	19.750956056160145	23.06798635128496	25.76609447553752
19	31.30116287149654	21.26333510911292	21.791924144547643	25.643577874842894
20	30.032049367485193	22.71780830601599	24.00734124347645	23.242801083022368
21	30.80646400023406	19.879628963655847	24.35965266238935	24.954254373720737
22	29.642861061298532	20.126612678090986	26.28096896611837	23.949557294492116
23	28.62804569564438	20.64819205726706	25.28998643174362	25.433775815344926
24	30.608852647272876	20.562247576182774	22.82862182843531	26.00027794810904
25	28.532226742265305	21.41791326799927	23.140825822842213	26.909034166893207
26	26.656077006255053	20.186103325990466	23.354528908431945	29.803290759322536
27	25.81778307125346	18.198347671636007	25.318512685039686	30.665356572070845
28	26.828941224946572	21.330566855663253	22.894268783135864	28.946223136254307
29	29.287258151620694	21.3864002916017	22.64515169505679	26.681189861720817
30	28.874297967687312	18.795143710144007	24.408537395601837	27.922020926566844
31	29.92026058854293	19.70902002567363	25.00789348248256	25.36282590330088
32	34.46050637756812	19.538472041224093	21.439368911504005	24.56165266970378
33	33.58283646045274	19.71298200529808	22.990392504178363	23.713789030070817
34	29.547407829115553	21.18372979542775	26.894466272581756	22.374396102874936
35	27.266587589616933	26.202948444282985	24.947732345723562	21.58273162037652
36	28.695277442194715	26.404643683933987	22.408712941775967	22.491365932095324
37	29.70984899371813	26.437497638050612	20.94979013698697	22.902863231244293
38	31.09422562803472	25.774932737776684	19.887613876437438	23.243227757751157
39	30.98767885290328	22.48795253426503	21.07547632138116	25.448892291450537
40	29.332668533470198	21.41334175304798	21.107415972507518	28.146573740974308
41	24.36635755098458	23.406644178876675	22.539823990579023	29.68717427955972
42	24.29431047535222	21.84830616228025	23.04683547544365	30.810547886923885
43	26.055623755786012	20.129416540594445	25.06616505972837	28.748794643891173
44	27.318459045930926	19.219624111754648	24.30211252753576	29.15980431477867
45	26.925674481315916	20.10789994355703	23.1049241920914	29.861501383035655
46	26.495647308231042	22.293937439732193	22.59443835586379	28.615976896172974
47	25.832107151434176	22.267117885351283	25.419573642229583	26.481201320984958
48	27.4786449298242	22.578346623235245	24.476927259273165	25.466081187667395
49	29.201862252330557	21.101137758641077	23.648142075370263	26.048857913658104
50	25.018377490104193	22.037505927731054	24.06622235604909	28.877894226115664
51	24.620899503472522	22.56249870473743	25.55653623017029	27.260065561619758
52	25.16228878077087	22.89140396709972	23.88195982674568	28.064347425383733
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	1.5
6	3.0
7	1.5
8	0.0
9	49.0
10	98.0
11	209.5
12	321.0
13	1646.0
14	3713.5
15	4456.0
16	4166.5
17	3877.0
18	3644.0
19	3411.0
20	3349.0
21	3287.0
22	3630.0
23	3973.0
24	3961.0
25	3949.0
26	4163.5
27	4378.0
28	6009.0
29	7640.0
30	7756.5
31	7873.0
32	8876.0
33	9879.0
34	12277.5
35	14676.0
36	17878.5
37	21081.0
38	21547.5
39	24726.0
40	27438.0
41	29568.0
42	31698.0
43	35257.0
44	38816.0
45	40802.5
46	42789.0
47	46316.0
48	49843.0
49	53657.5
50	57472.0
51	64113.5
52	70755.0
53	80560.0
54	90365.0
55	100717.0
56	111069.0
57	129102.5
58	147136.0
59	153540.5
60	159945.0
61	162498.5
62	165052.0
63	161157.5
64	138313.0
65	119363.0
66	103369.5
67	87376.0
68	71664.0
69	55952.0
70	48977.0
71	42002.0
72	36400.0
73	30798.0
74	26800.5
75	22803.0
76	17530.0
77	12257.0
78	8201.0
79	4145.0
80	2779.0
81	1413.0
82	1064.5
83	716.0
84	461.5
85	207.0
86	108.5
87	10.0
88	10.5
89	8.5
90	6.0
91	4.5
92	3.0
93	3.5
94	4.0
95	2.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1640594.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.651639420344361
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.42940778909029	6.990625274093809
2	10.286018233982126	1.9855387813096554
3	4.042565163571042	1.1705214387609932
4	2.035054620370209	0.7856645358607617
5	1.1619391979422518	0.5607309083451375
6	0.6959504646608918	0.40302377635968234
7	0.4550996217590452	0.3074720214687387
8	0.3282458842516581	0.2534488732807277
9	0.27383441838536055	0.23786559604216947
>10	3.1840811312091106	7.904017348432069
>50	2.2647335770604347	16.1658263299361
>100	2.69794965903601	47.423878682104345
>500	0.0979382891594948	6.32968798381397
>1k	0.04503731545290637	7.144478860014226
>5k	7.148780230620058E-4	0.48215417417964224
>10k+	0.0014297560461240116	1.855065415997977
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TGTCACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	14609	0.890470158979004	No Hit
TGTCACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	12277	0.7483265207601637	No Hit
TGTCACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	6988	0.4259432863950496	No Hit
TGTCACGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG	4312	0.26283163293294987	No Hit
TGTCACAGGAGTCTAGAACACACAAAACTCGCAGGCAATCCCGGGGCCAGCC	3446	0.21004587362869787	No Hit
TGTCACGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	3441	0.20974110596527845	No Hit
TGTCACGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	3020	0.18407966870535916	No Hit
TGTCACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	2977	0.18145866679995173	No Hit
TGTCACGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	2938	0.17908147902527988	No Hit
TGTCACGGGGACTGTCGAGTCGCGTCCACCCGCGAGCACAGCTTCTTTGCAG	2876	0.17530235999887847	No Hit
TGTCACGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	2809	0.17121847330905757	No Hit
TGTCACGGGGACAGCATGCTCTCTCCCTATAGGCACGGCCTCCGCCGTGTGG	2638	0.16079541922011173	No Hit
TGTCACGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	2509	0.15293241350388945	No Hit
TGTCACGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC	2408	0.14677610670281618	No Hit
TGTCACGGGGAAGTGTTCCTGGTCTGTGTGTGTGGCTGTCCGTGTTGTCGCT	2145	0.1307453276069521	No Hit
TGTCACGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACG	2101	0.1280633721688608	No Hit
TGTCACGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	1971	0.1201394129199546	No Hit
TGTCACGGCACGACGCTACTCAGACTACCCAGATGCTTACACCACATGAAAC	1971	0.1201394129199546	No Hit
TGTCACGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG	1957	0.11928606346238008	No Hit
TGTCACGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	1949	0.11879843520090894	No Hit
TGTCACGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTT	1938	0.1181279463413861	No Hit
TGTCACGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	1641	0.10002474713426965	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	3.6572119610336256E-4	0.0	0.0	0.0	0.0
8	4.2667472878725633E-4	0.0	0.0	0.0	0.0
9	4.2667472878725633E-4	0.0	0.0	0.0	0.0
10	0.002255280709304069	0.0	0.0	0.0	0.0
11	0.0037181654937175194	0.0	0.0	0.0	0.0
12	0.0054248644088665445	0.0	0.0	0.0	0.0
13	0.008777308706480701	0.0	0.0	0.0	0.0
14	0.013897405451927778	0.0	0.0	0.0	0.0
15	0.019870851654949367	0.0	0.0	0.0	0.0
16	0.03151297639757308	0.0	0.0	0.0	0.0
17	0.03773023673133024	0.0	0.0	0.0	0.0
18	0.04358177586898404	0.0	0.0	0.0	0.0
19	0.052298131042780846	0.0	0.0	0.0	0.0
20	0.058088716647750754	0.0	0.0	0.0	0.0
21	0.0644278840468757	0.0	0.0	0.0	0.0
22	0.07064514438063287	0.0	0.0	0.0	0.0
23	0.07649668351828667	0.0	0.0	0.0	0.0
24	0.08247012972130825	0.0	0.0	0.0	0.0
25	0.08722450527065197	0.0	0.0	0.0	0.0
26	0.09167411315657621	0.0	0.0	0.0	0.0
27	0.09612372104250046	0.0	0.0	0.0	0.0
28	0.1025238419743093	0.0	0.0	0.0	0.0
29	0.10685154279486576	0.0	0.0	0.0	0.0
30	0.11221545367104842	0.0	0.0	0.0	0.002560048372723538
31	0.11812794634138611	0.0	0.0	0.0	0.002560048372723538
32	0.12471092787124663	0.0	0.0	0.0	0.002560048372723538
33	0.1287948145610675	0.0	0.0	0.0	0.002560048372723538
34	0.1338539577738307	0.0	0.0	0.0	0.002560048372723538
35	0.14226554528420804	0.0	0.0	0.0	0.002560048372723538
36	0.15543150834392908	0.0	0.0	0.0	0.002560048372723538
37	0.16396500291967422	0.0	0.0	0.0	0.002560048372723538
38	0.17164514803784484	0.0	0.0	0.0	0.002560048372723538
39	0.1776795477735503	0.0	0.0	0.0	0.002560048372723538
40	0.18353108691120412	0.0	0.0	0.0	0.002560048372723538
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTAT	145	0.0	46.000004	42
CGTATAC	140	0.0	46.000004	44
ATTACGT	40	5.6134013E-9	46.000004	24
CAATTGG	20	6.312246E-4	46.000004	40
CGATTAG	150	0.0	46.000004	30
TATGCGT	35	1.01992555E-7	46.000004	15
TAGTTCG	20	6.312246E-4	46.000004	29
TACGCTA	20	6.312246E-4	46.000004	34
CCCGTAA	35	1.01992555E-7	46.000004	41
CGTAGTA	20	6.312246E-4	46.000004	12
GCGATAA	35	1.01992555E-7	46.000004	36
ATACGCA	20	6.312246E-4	46.000004	14
TATCGTT	20	6.312246E-4	46.000004	21
CGTTTAT	20	6.312246E-4	46.000004	15
CCGTTCA	40	5.6134013E-9	46.000004	33
GTTATCG	35	1.01992555E-7	46.000004	29
GTACGTT	35	1.01992555E-7	46.000004	19
TACGTAT	20	6.312246E-4	46.000004	28
GTACGAA	20	6.312246E-4	46.000004	21
ACGGTAC	35	1.01992555E-7	46.000004	5
>>END_MODULE