FastQCFastQC Report
Fri 17 Jun 2016
SRR1527109_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527109_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1592820
Sequences flagged as poor quality0
Sequence length52
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC132640.8327369068695772No Hit
TGTCACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA121630.763614218806896No Hit
TGTCACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT68940.43281726748785176No Hit
TGTCACGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG40350.25332429276377744No Hit
TGTCACGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC33590.21088384123755352No Hit
TGTCACAGGAGTCTAGAACACACAAAACTCGCAGGCAATCCCGGGGCCAGCC33130.20799588151831344No Hit
TGTCACGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC30530.19167263093130424No Hit
TGTCACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC29500.1852061124295275No Hit
TGTCACGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG28810.18087417285066737No Hit
TGTCACGGGGACTGTCGAGTCGCGTCCACCCGCGAGCACAGCTTCTTTGCAG28750.18049748245250563No Hit
TGTCACGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT27570.17308923795532452No Hit
TGTCACGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC24540.1540663728481561No Hit
TGTCACGGGGACAGCATGCTCTCTCCCTATAGGCACGGCCTCCGCCGTGTGG22630.142075061840007No Hit
TGTCACGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC22050.13843372132444345No Hit
TGTCACGGGGAAGTGTTCCTGGTCTGTGTGTGTGGCTGTCCGTGTTGTCGCT21540.13523185294006856No Hit
TGTCACGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACG21290.1336623096143946No Hit
TGTCACGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG19390.1217337803392725No Hit
TGTCACGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG19150.12022701874662549No Hit
TGTCACGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTT19130.12010145528057158No Hit
TGTCACGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG19010.11934807448424807No Hit
TGTCACGGCACGACGCTACTCAGACTACCCAGATGCTTACACCACATGAAAC18660.11715071382830453No Hit
TGTCACGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT17730.11131201265679738No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATATCG253.4177538E-546.021
CGTATAT551.8189894E-1246.041
ACGCATA253.4177538E-546.011
CCGATAC253.4177538E-546.016
CGTGATA301.8618593E-646.032
ATTACGT253.4177538E-546.024
CGATTAG1000.046.030
TACGGGA206.312192E-446.030
TACGGAA253.4177538E-546.044
GTAATCG850.046.09
CGTAGTA405.6134013E-946.012
CGTAGGT301.8618593E-646.037
TATTCCG253.4177538E-546.042
TATTAGC850.046.041
CGAATAG206.312192E-446.037
CGAATAA206.312192E-446.035
CACGTAA206.312192E-446.046
CCCGGTA850.046.014
CGTCATA206.312192E-446.039
TCGAATA206.312192E-446.036
Produced by FastQC (version 0.11.5)