##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527109_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1592820
Sequences flagged as poor quality	0
Sequence length	52
%GC	57
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.33174558330508	33.0	31.0	34.0	31.0	34.0
2	32.62233083462036	34.0	31.0	34.0	31.0	34.0
3	31.72847402719705	31.0	31.0	34.0	30.0	34.0
4	36.090949385366834	37.0	35.0	37.0	35.0	37.0
5	36.06265993646489	37.0	35.0	37.0	35.0	37.0
6	36.28289009429816	37.0	37.0	37.0	35.0	37.0
7	36.32537386522018	37.0	37.0	37.0	35.0	37.0
8	36.577732574929996	37.0	37.0	37.0	35.0	37.0
9	38.62134767268115	39.0	39.0	39.0	38.0	39.0
10	38.081965947188	39.0	38.0	39.0	37.0	39.0
11	37.654233372258005	39.0	38.0	39.0	35.0	39.0
12	36.97279479162743	39.0	35.0	39.0	34.0	39.0
13	36.7365640813149	39.0	35.0	39.0	33.0	39.0
14	37.413584083575046	40.0	36.0	40.0	33.0	40.0
15	37.57137718009568	40.0	36.0	40.0	33.0	40.0
16	37.7001305860047	40.0	36.0	40.0	33.0	40.0
17	37.6054777062066	40.0	36.0	40.0	33.0	40.0
18	37.598900064037366	40.0	36.0	40.0	33.0	40.0
19	37.55668499893271	40.0	36.0	40.0	33.0	40.0
20	37.41322246079281	40.0	36.0	40.0	33.0	40.0
21	33.19103476852375	39.0	33.0	40.0	10.0	40.0
22	33.8612228625959	39.0	33.0	40.0	16.0	40.0
23	35.875631270325584	38.0	34.0	40.0	29.0	40.0
24	36.72693964164187	38.0	35.0	40.0	31.0	40.0
25	37.03444457000791	39.0	35.0	40.0	32.0	40.0
26	37.09891952637461	39.0	35.0	40.0	33.0	40.0
27	37.02983074044776	39.0	35.0	40.0	33.0	40.0
28	36.917692520184325	39.0	35.0	40.0	32.0	40.0
29	36.846574628646046	39.0	35.0	40.0	32.0	40.0
30	36.74269283409299	39.0	35.0	40.0	32.0	40.0
31	36.59128966235984	39.0	35.0	40.0	31.0	40.0
32	36.55494406147587	38.0	35.0	40.0	31.0	40.0
33	36.203825918810665	38.0	35.0	40.0	31.0	40.0
34	35.98891149031278	38.0	35.0	40.0	30.0	40.0
35	35.85683316382266	38.0	34.0	40.0	30.0	40.0
36	35.80303110207054	38.0	34.0	40.0	30.0	40.0
37	35.72942956517372	38.0	34.0	40.0	30.0	40.0
38	35.62668411998845	37.0	34.0	40.0	30.0	40.0
39	35.436193669090045	37.0	34.0	40.0	29.0	40.0
40	30.590868396931228	35.0	29.0	39.0	8.0	40.0
41	31.383538001783002	35.0	28.0	39.0	12.0	40.0
42	33.1343924611695	35.0	30.0	39.0	23.0	40.0
43	33.989001268191004	35.0	32.0	39.0	26.0	40.0
44	34.80914541505004	36.0	33.0	39.0	28.0	40.0
45	35.09702665712384	36.0	34.0	40.0	29.0	40.0
46	35.1579023367361	35.0	34.0	40.0	30.0	40.0
47	35.108258936979695	35.0	34.0	40.0	29.0	40.0
48	34.980152182920854	35.0	34.0	40.0	29.0	40.0
49	34.84091297196168	35.0	34.0	39.0	29.0	40.0
50	34.71487424818875	35.0	34.0	39.0	29.0	40.0
51	34.56744139325222	35.0	34.0	39.0	29.0	40.0
52	34.146205472055854	35.0	33.0	38.0	27.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	3.0
14	6.0
15	41.0
16	83.0
17	195.0
18	365.0
19	671.0
20	1227.0
21	1938.0
22	3272.0
23	4794.0
24	6866.0
25	9301.0
26	12573.0
27	16348.0
28	22031.0
29	28727.0
30	38148.0
31	51706.0
32	72789.0
33	87498.0
34	156528.0
35	176458.0
36	201179.0
37	226480.0
38	345679.0
39	127912.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	100.0	0.0	0.0	0.0
3	0.0	0.0	100.0	0.0
4	0.0	0.0	0.0	100.0
5	0.0	100.0	0.0	0.0
6	0.0	0.0	0.0	100.0
7	99.15131653294158	0.7187252796926207	0.039552491806983836	0.0904056955588202
8	99.55569367536822	0.3065004206376113	0.05248552881053729	0.08532037518363657
9	98.0994085960749	0.8791953893095265	0.3820896272020693	0.6393063874135182
10	63.46774902374405	25.28245501688829	4.168958199922151	7.080837759445512
11	39.357554525935136	23.090116899586896	18.510503383935408	19.04182519054256
12	32.98545975063096	14.678243618236838	26.5107168418277	25.825579789304502
13	26.532313757988973	14.963021559247123	29.728908476789595	28.77575620597431
14	25.12882811617132	15.423902261398023	32.98263457264474	26.46463504978591
15	27.026657123843243	18.028779146419556	29.96195426978566	24.982609459951533
16	32.80201152672618	18.738714983488407	24.367097349355234	24.09217614043018
17	32.46481083863839	19.41273966926583	23.570648284175235	24.551801207920544
18	31.301590889114905	19.78120566040105	23.031353197473663	25.885850253010386
19	31.294119862884695	21.233472708780653	21.795557564571013	25.67684986376364
20	29.95052799437476	22.83239788551123	23.898871184440175	23.31820293567384
21	35.05882648384626	19.3076430481787	23.177257945028316	22.456272522946723
22	29.680880451023974	20.151429540061024	26.286272146256323	23.88141786265868
23	28.592182418603485	20.658768724651875	25.295450835624866	25.453598021119777
24	30.660024359312416	20.66153112090506	22.710538541705905	25.967905978076615
25	28.60919626825379	21.377054532213307	23.11485290239952	26.898896297133383
26	26.67520498235833	20.29582752602303	23.27682977360907	29.752137718009568
27	25.875428485327912	18.18190379327231	25.348564181765674	30.59410353963411
28	26.814957120076343	21.31772579450283	22.88626461244836	28.98105247297246
29	29.11471478258686	21.449379088660365	22.689129970743714	26.746776158009066
30	28.94463906781683	18.84299544204618	24.307140794314485	27.905224695822504
31	29.96929973254982	19.72463931894376	25.01136349367788	25.29469745482854
32	34.481046194799156	19.497432227119198	21.404364586080035	24.617156992001608
33	33.54647731695985	19.720746851496088	22.999146168430833	23.73362966311322
34	29.541065531572936	21.17640411345915	26.845406260594416	22.4371240943735
35	27.241684559460577	26.26335681370148	24.94801672505368	21.546941901784255
36	28.83640336007835	26.404364586080035	22.32035007094336	22.438881982898256
37	29.569066184502958	26.563390715837322	20.974246933112344	22.89329616654738
38	31.204969801986415	25.7317210984292	19.78534925478083	23.277959844803554
39	30.655817983199608	22.50134980726008	21.12661819916877	25.71621401037154
40	29.5239889001896	26.134842606195303	19.544706872088497	24.7964616215266
41	24.36031692218832	23.449919011564397	22.512022701874663	29.677741364372622
42	24.344182016800392	21.851998342562247	23.032483268668148	30.77133637196921
43	26.026167426325635	20.1851433306965	25.086576009844176	28.702113233133687
44	27.363041649401694	19.281525847239486	24.20794565613189	29.147486847226933
45	26.917479690109364	20.166873846385656	23.044349016210244	29.871297447294737
46	26.419243856807423	22.320161725744278	22.6183121758893	28.642282241559
47	25.63516279303374	22.331525219422158	25.409776371466958	26.62353561607715
48	27.501349807260077	22.655102271443102	24.43169975263997	25.411848168656846
49	29.241157192903156	21.173767280672013	23.597958338041963	25.987117188382868
50	24.9799726271644	21.974359940231793	24.246933112341633	28.798734320262177
51	24.685212390602832	22.606320864881155	25.456423199105988	27.252043545410025
52	25.093230873545036	23.016473926746272	23.836277796612297	28.054017403096392
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	3.0
6	6.0
7	3.0
8	0.0
9	36.0
10	72.0
11	195.5
12	319.0
13	1551.5
14	3609.0
15	4434.0
16	4183.5
17	3933.0
18	3705.5
19	3478.0
20	3366.0
21	3254.0
22	3579.5
23	3905.0
24	3929.5
25	3954.0
26	4165.0
27	4376.0
28	5863.5
29	7351.0
30	7423.0
31	7495.0
32	8471.0
33	9447.0
34	11287.5
35	13128.0
36	16380.0
37	19632.0
38	20441.0
39	23973.0
40	26696.0
41	28816.5
42	30937.0
43	34059.5
44	37182.0
45	39172.5
46	41163.0
47	44858.0
48	48553.0
49	52196.5
50	55840.0
51	61958.0
52	68076.0
53	77948.5
54	87821.0
55	96762.5
56	105704.0
57	124396.5
58	143089.0
59	148781.0
60	154473.0
61	156365.5
62	158258.0
63	156275.0
64	135632.0
65	116972.0
66	101904.0
67	86836.0
68	71322.0
69	55808.0
70	48406.0
71	41004.0
72	35520.5
73	30037.0
74	26193.5
75	22350.0
76	17224.5
77	12099.0
78	8225.0
79	4351.0
80	2902.5
81	1454.0
82	1093.0
83	732.0
84	485.0
85	238.0
86	131.5
87	25.0
88	14.5
89	4.0
90	4.0
91	3.0
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1592820.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.59685473836106
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.17341773650051	7.022346607266082
2	9.202059945802453	1.7662166518711362
3	3.740289349312543	1.0768504069437446
4	2.0045205555004535	0.7694837036478669
5	1.1526801049987292	0.5531051763735789
6	0.7776862992377829	0.44780054674791564
7	0.543691362540653	0.3652408920162972
8	0.37457064543106533	0.2875760058764864
9	0.28034132913359133	0.24213555115687294
>10	3.5328927633766725	8.695325647874249
>50	2.374196462511993	16.787159874025498
>100	2.7031153678977855	46.776888171442046
>500	0.0941904138150224	6.036111665402085
>1k	0.044105035040367634	6.855030758565279
>5k	7.47542966785892E-4	0.49457978346623377
>10k+	0.001495085933571784	1.8241485573246194
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TGTCACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	13264	0.8327369068695772	No Hit
TGTCACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	12163	0.763614218806896	No Hit
TGTCACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	6894	0.43281726748785176	No Hit
TGTCACGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG	4035	0.25332429276377744	No Hit
TGTCACGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	3359	0.21088384123755352	No Hit
TGTCACAGGAGTCTAGAACACACAAAACTCGCAGGCAATCCCGGGGCCAGCC	3313	0.20799588151831344	No Hit
TGTCACGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	3053	0.19167263093130424	No Hit
TGTCACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	2950	0.1852061124295275	No Hit
TGTCACGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	2881	0.18087417285066737	No Hit
TGTCACGGGGACTGTCGAGTCGCGTCCACCCGCGAGCACAGCTTCTTTGCAG	2875	0.18049748245250563	No Hit
TGTCACGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	2757	0.17308923795532452	No Hit
TGTCACGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	2454	0.1540663728481561	No Hit
TGTCACGGGGACAGCATGCTCTCTCCCTATAGGCACGGCCTCCGCCGTGTGG	2263	0.142075061840007	No Hit
TGTCACGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC	2205	0.13843372132444345	No Hit
TGTCACGGGGAAGTGTTCCTGGTCTGTGTGTGTGGCTGTCCGTGTTGTCGCT	2154	0.13523185294006856	No Hit
TGTCACGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACG	2129	0.1336623096143946	No Hit
TGTCACGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	1939	0.1217337803392725	No Hit
TGTCACGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG	1915	0.12022701874662549	No Hit
TGTCACGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTT	1913	0.12010145528057158	No Hit
TGTCACGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	1901	0.11934807448424807	No Hit
TGTCACGGCACGACGCTACTCAGACTACCCAGATGCTTACACCACATGAAAC	1866	0.11715071382830453	No Hit
TGTCACGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	1773	0.11131201265679738	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	1.2556346605391695E-4	0.0	0.0	0.0	0.0
8	1.2556346605391695E-4	0.0	0.0	0.0	0.0
9	1.8834519908087543E-4	0.0	0.0	0.0	0.0
10	0.0022601423889705053	0.0	0.0	0.0	0.0
11	0.004771411710048844	0.0	0.0	0.0	0.0
12	0.00659208196783064	0.0	0.0	0.0	0.0
13	0.010735676347609899	0.0	0.0	0.0	0.0
14	0.015130397659496992	0.0	0.0	0.0	0.0
15	0.02059240843284238	0.0	0.0	0.0	0.0
16	0.030825830916236613	0.0	0.0	0.0	0.0
17	0.035597242626285455	0.0	0.0	0.0	0.0
18	0.040682563001469094	0.0	0.0	0.0	0.0
19	0.047462990168380605	0.0	0.0	0.0	0.0
20	0.05411785386923821	0.0	0.0	0.0	0.0
21	0.0600193367737723	0.0	0.0	0.0	0.0
22	0.06661141874160294	0.0	0.0	0.0	0.0
23	0.072575683379164	0.0	0.0	0.0	0.0
24	0.07828882108461722	0.0	0.0	0.0	0.0
25	0.08280910586255823	0.0	0.0	0.0	0.0
26	0.08764329930563403	0.0	0.0	0.0	0.0
27	0.092226365816602	0.0	0.0	0.0	0.0
28	0.09787672178902826	0.0	0.0	0.0	0.0
29	0.10252257003302319	0.0	0.0	0.0	0.0
30	0.10773345387426074	0.0	0.0	0.0	0.002511269321078339
31	0.11256764731733655	0.0	0.0	0.0	0.002511269321078339
32	0.11853191195489761	0.0	0.0	0.0	0.002511269321078339
33	0.12380557752916212	0.0	0.0	0.0	0.002511269321078339
34	0.1292675883025075	0.0	0.0	0.0	0.002511269321078339
35	0.13661305106666163	0.0	0.0	0.0	0.002511269321078339
36	0.1486043620748107	0.0	0.0	0.0	0.002511269321078339
37	0.15701711430042314	0.0	0.0	0.0	0.002511269321078339
38	0.16505317612787385	0.0	0.0	0.0	0.002511269321078339
39	0.17151969462965055	0.0	0.0	0.0	0.002511269321078339
40	0.1791790660589395	0.0	0.0	0.0	0.002511269321078339
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATATCG	25	3.4177538E-5	46.0	21
CGTATAT	55	1.8189894E-12	46.0	41
ACGCATA	25	3.4177538E-5	46.0	11
CCGATAC	25	3.4177538E-5	46.0	16
CGTGATA	30	1.8618593E-6	46.0	32
ATTACGT	25	3.4177538E-5	46.0	24
CGATTAG	100	0.0	46.0	30
TACGGGA	20	6.312192E-4	46.0	30
TACGGAA	25	3.4177538E-5	46.0	44
GTAATCG	85	0.0	46.0	9
CGTAGTA	40	5.6134013E-9	46.0	12
CGTAGGT	30	1.8618593E-6	46.0	37
TATTCCG	25	3.4177538E-5	46.0	42
TATTAGC	85	0.0	46.0	41
CGAATAG	20	6.312192E-4	46.0	37
CGAATAA	20	6.312192E-4	46.0	35
CACGTAA	20	6.312192E-4	46.0	46
CCCGGTA	85	0.0	46.0	14
CGTCATA	20	6.312192E-4	46.0	39
TCGAATA	20	6.312192E-4	46.0	36
>>END_MODULE