FastQCFastQC Report
Fri 17 Jun 2016
SRR1527108_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527108_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3446206
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC866382.514011060279043No Hit
CCATCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG508001.4740848341625545No Hit
CCATCTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT278880.8092377530536481No Hit
CCATCTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT161150.4676156909946765No Hit
CCATCTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC113480.329289659410958No Hit
CCATCTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC98870.28689521171978694No Hit
CCATCTGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCT86160.25001407344772775No Hit
CCATCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT84700.24577753042041017No Hit
CCATCTGGGATTGTTAATTATTCTTTTGGATTCTTTTGAACTTGTAATAC56090.16275869753578281No Hit
CCATCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTG53580.15547532561895602No Hit
CCATCTGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGC51840.15042629488776935No Hit
CCATCTGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGTCGGC51100.1482790059561152No Hit
CCATCTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT49070.14238847010306407No Hit
CCATCTGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGC47490.13780371805980257No Hit
CCATCTGGGGGGGCTGGTGAGGTGGCTCAGTGGGTAAGAGCACCCGACTG43690.12677709922157876No Hit
CCATCTGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCT43210.1253842631578031No Hit
CCATCTGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA43110.12509408897784985No Hit
CCATCTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC41700.1210026330405089No Hit
CCATCTGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC40690.11807187382298098No Hit
CCATCTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC39170.11366122628769144No Hit
CCATCTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA39090.11342908694372884No Hit
CCATCTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCG37850.10983092711230842No Hit
CCATCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGC37570.1090184394084393No Hit
CCATCTGGGGCTCAGCCCGTAGCCCGTCGGTTCCGGAGTAAGTTCCAGGT34920.10132882363967795No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG207.858674E-444.00000442
CGATTAC254.444699E-544.030
CGTACAA254.444699E-544.022
TCGGTAC351.4472062E-744.016
CTGGGAT320150.040.8939555
CCATCTG3679550.040.8873371
CATCTGG3654850.040.8807452
TCTGGGA988550.040.857624
ATCTGGG3586350.040.8371163
TCTGGGG2101850.040.4537934
CTGGGGG1012450.040.3603175
CTGGGGC474050.040.083115
TGGGATC102750.040.060346
TGGGGGG686000.039.9046676
TGGGGGC177150.039.8396846
TGGGATT115900.039.6911136
CCGATTT1000.039.641
TCTGGGC328100.039.4404144
TGGGACT70300.039.3997156
TCGAATA950.039.3684240
Produced by FastQC (version 0.11.5)