##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527108_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3446206
Sequences flagged as poor quality	0
Sequence length	50
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.61329908891111	34.0	31.0	34.0	31.0	34.0
2	33.05266632348734	34.0	31.0	34.0	31.0	34.0
3	32.98814725527145	34.0	31.0	34.0	31.0	34.0
4	36.473913631396385	37.0	37.0	37.0	35.0	37.0
5	36.41688918190033	37.0	37.0	37.0	35.0	37.0
6	36.39369759091592	37.0	37.0	37.0	35.0	37.0
7	36.53869211532915	37.0	37.0	37.0	35.0	37.0
8	36.67699667402355	37.0	37.0	37.0	35.0	37.0
9	38.75832785387757	39.0	39.0	39.0	39.0	39.0
10	38.266721142032715	39.0	39.0	39.0	37.0	39.0
11	38.0193528767578	39.0	38.0	39.0	35.0	39.0
12	37.21266285300415	39.0	35.0	39.0	35.0	39.0
13	37.05772173805048	39.0	35.0	39.0	34.0	39.0
14	37.71432177879094	40.0	36.0	40.0	34.0	40.0
15	37.677421489023004	40.0	36.0	40.0	34.0	40.0
16	37.544728318620535	40.0	35.0	40.0	33.0	40.0
17	37.396847431639316	40.0	35.0	40.0	33.0	40.0
18	37.378017738927966	40.0	35.0	40.0	33.0	40.0
19	37.373645974732796	40.0	35.0	40.0	33.0	40.0
20	37.206289757489834	40.0	35.0	40.0	32.0	40.0
21	37.45154178247034	40.0	35.0	40.0	33.0	40.0
22	37.327222458552974	40.0	35.0	40.0	33.0	40.0
23	37.424754643222144	40.0	35.0	40.0	33.0	40.0
24	37.49013117613979	40.0	35.0	40.0	34.0	40.0
25	37.45805590263612	40.0	35.0	40.0	34.0	40.0
26	37.32995996176665	39.0	35.0	40.0	34.0	40.0
27	37.25139356149923	39.0	35.0	40.0	34.0	40.0
28	37.09908664775118	39.0	35.0	40.0	33.0	40.0
29	37.30774770863959	40.0	35.0	40.0	34.0	40.0
30	37.33478875029525	40.0	35.0	40.0	34.0	40.0
31	37.32064537059015	40.0	35.0	40.0	34.0	40.0
32	36.61171705928201	40.0	35.0	40.0	32.0	40.0
33	36.82903082404244	39.0	35.0	40.0	32.0	40.0
34	36.651568420460066	39.0	35.0	40.0	31.0	40.0
35	36.71828758930836	39.0	35.0	40.0	31.0	40.0
36	36.67045353643978	39.0	35.0	40.0	32.0	40.0
37	36.76370013864522	39.0	35.0	40.0	32.0	40.0
38	36.59075719791562	39.0	35.0	40.0	32.0	40.0
39	36.590374167998085	39.0	35.0	40.0	32.0	40.0
40	36.51173348314059	39.0	35.0	40.0	32.0	40.0
41	36.41301709764303	39.0	35.0	40.0	32.0	40.0
42	36.34690961596608	39.0	35.0	40.0	31.0	40.0
43	36.28287658950161	39.0	35.0	40.0	31.0	40.0
44	36.1784347772594	38.0	35.0	40.0	31.0	40.0
45	36.085520714664185	38.0	35.0	40.0	31.0	40.0
46	35.75808671913403	38.0	35.0	40.0	30.0	40.0
47	35.698814000091694	38.0	35.0	40.0	30.0	40.0
48	35.619149580727324	37.0	35.0	40.0	30.0	40.0
49	35.37769245367224	37.0	35.0	40.0	29.0	40.0
50	35.04544040605814	36.0	34.0	40.0	28.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	3.0
13	0.0
14	1.0
15	22.0
16	75.0
17	185.0
18	447.0
19	1014.0
20	1655.0
21	2630.0
22	4257.0
23	6279.0
24	8924.0
25	12487.0
26	17482.0
27	22913.0
28	30305.0
29	39843.0
30	52489.0
31	69866.0
32	90013.0
33	124859.0
34	284078.0
35	382908.0
36	233216.0
37	402165.0
38	961844.0
39	696246.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	0.0	100.0
2	0.0	0.0	0.0	100.0
3	0.0	100.0	0.0	0.0
4	0.0	0.0	100.0	0.0
5	0.0	0.0	0.0	100.0
6	0.0	0.0	100.0	0.0
7	99.24017890979239	0.5071374143043104	0.19891440035795888	0.05376927554533884
8	99.17718209532454	0.5176997544546089	0.1745107518238898	0.13060739839696175
9	97.7297062334637	1.1805736511398333	0.41950481195842615	0.6702153034380418
10	57.55332095643732	27.51446083025797	5.7554017374469195	9.176816475857798
11	40.59121828468757	17.42356667012941	21.373504659907155	20.611710385275867
12	37.34007775507326	12.079196658586284	26.472358297791832	24.108367288548624
13	27.431354945119356	12.906860472066963	27.35518422288163	32.306600359932055
14	23.76050648162066	15.55884935491378	32.99518368896114	27.685460474504424
15	28.856516412541794	18.122306095456857	30.222105120819826	22.799072371181524
16	36.18106404550395	17.596452446545563	26.13149068860074	20.090992819349744
17	32.2715763364117	18.591894970875217	25.078245467624395	24.05828322508869
18	32.79267112877176	18.88099550636265	26.935853515431173	21.39047984943442
19	31.950411554039427	24.12006130800074	22.676328693061297	21.253198444898537
20	34.0687991373702	22.870803428465972	22.77092547572606	20.289471958437773
21	31.930621674966613	23.645568488941173	24.209318885754364	20.214490950337847
22	28.176435186985337	20.35566068888511	29.580239834763216	21.887664289366334
23	32.36890075636802	19.107853680250106	26.83580145818329	21.687444105198587
24	35.44318012330081	18.469905745622867	22.842598498174517	23.244315632901806
25	29.103077413248073	18.858971286104197	22.294604559332782	29.74334674131494
26	24.77010950593203	19.379717869448314	27.313108966788402	28.53706365783125
27	22.59629865423019	17.619753433195807	26.65360109059064	33.130346821983366
28	24.28215260492263	24.60659635552837	22.44920936241188	28.66204167713712
29	31.64503224705662	22.683031716618217	21.704535364397834	23.96740067192733
30	30.826276780900503	19.026924101461145	26.391631840928838	23.755167276709518
31	33.99538506984202	18.298586909778464	24.32779700342928	23.378231016950235
32	36.961835711504186	17.62520870777893	24.645131486626163	20.76782409409072
33	35.466219953189096	18.12802252680194	25.713030503690142	20.692727016318816
34	28.029432947421018	20.288949644913856	31.763568399567525	19.9180490080976
35	24.94044174956459	28.086394138945842	28.794912434137714	18.17825167735185
36	25.572499148338785	32.30723874312795	23.214282605276644	18.905979503256624
37	30.90726439452546	28.58227279506797	19.884446838059013	20.62601597234756
38	30.247988657671655	28.31281705156337	20.38464908946244	21.05454520130253
39	31.26545540225976	25.257166866983578	20.1148451369419	23.362532593814763
40	27.7979900214903	23.103581155624475	20.270146358052884	28.828282464832338
41	23.03692814648921	26.447635457659814	20.997613027195705	29.51782336865527
42	21.96708496241954	24.27217061313224	21.93867691020212	31.8220675142461
43	22.916738001152574	20.275485562964025	24.19655122183642	32.61122521404698
44	24.596817485663944	19.250996603221047	23.99821716983837	32.15396874127664
45	28.340992964436833	21.548247551075008	22.652882619321073	27.457876865167087
46	26.225739262249558	25.318103444773758	21.949877633548315	26.506279659428365
47	23.517224449147843	22.551553795681397	25.672348083660694	28.25887367151006
48	25.918589892769035	21.874867608030396	27.870939810330547	24.33560268887002
49	29.354455305341588	20.32823922887953	24.320687736020425	25.996617729758466
50	24.754875361484487	20.386535221632137	26.169474488756624	28.689114928126756
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	2.0
8	4.0
9	58.0
10	112.0
11	433.5
12	755.0
13	2725.0
14	4695.0
15	5758.0
16	6821.0
17	6707.0
18	6593.0
19	7879.5
20	9166.0
21	10171.5
22	11177.0
23	11469.5
24	11762.0
25	11891.5
26	12021.0
27	14733.0
28	17445.0
29	21339.5
30	25234.0
31	25892.0
32	26550.0
33	30619.0
34	34688.0
35	35801.0
36	36914.0
37	45056.0
38	53198.0
39	58372.5
40	63547.0
41	66230.5
42	68914.0
43	75393.0
44	81872.0
45	89691.0
46	97510.0
47	105829.0
48	114148.0
49	124128.5
50	134109.0
51	145672.0
52	157235.0
53	170886.5
54	184538.0
55	210277.0
56	236016.0
57	277749.5
58	319483.0
59	335411.5
60	351340.0
61	371567.0
62	391794.0
63	424557.5
64	457321.0
65	333142.0
66	208963.0
67	162391.5
68	115820.0
69	92511.5
70	69203.0
71	60082.5
72	50962.0
73	41929.0
74	32896.0
75	31206.5
76	29517.0
77	21498.5
78	13480.0
79	9658.5
80	5837.0
81	4284.0
82	2731.0
83	2002.0
84	1273.0
85	816.0
86	359.0
87	273.0
88	187.0
89	100.0
90	13.0
91	8.0
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3446206.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	6.365316458666763
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.79938438874645	4.1246858796116195
2	11.016538585119893	1.402475087468022
3	5.090349745422388	0.9720506104472189
4	2.740077579204224	0.6976584365172971
5	1.608831417470684	0.5120360550423161
6	0.9857690209670582	0.37648390641632623
7	0.6923255995538669	0.30848100735175943
8	0.4815393025789069	0.24521200385603445
9	0.3895355157748926	0.22315651468174533
>10	4.8587482970580265	8.256856797375852
>50	3.5352816466928902	16.407340124440132
>100	3.5214645068713435	40.36404437556217
>500	0.17819344142468982	7.76878536726884
>1k	0.09624351649141001	11.031049863429486
>5k	0.003335171363563713	1.4628284297955005
>10k+	0.0023822652596883665	5.846855540735679
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCATCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	86638	2.514011060279043	No Hit
CCATCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	50800	1.4740848341625545	No Hit
CCATCTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	27888	0.8092377530536481	No Hit
CCATCTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	16115	0.4676156909946765	No Hit
CCATCTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	11348	0.329289659410958	No Hit
CCATCTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC	9887	0.28689521171978694	No Hit
CCATCTGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCT	8616	0.25001407344772775	No Hit
CCATCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT	8470	0.24577753042041017	No Hit
CCATCTGGGATTGTTAATTATTCTTTTGGATTCTTTTGAACTTGTAATAC	5609	0.16275869753578281	No Hit
CCATCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTG	5358	0.15547532561895602	No Hit
CCATCTGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGC	5184	0.15042629488776935	No Hit
CCATCTGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGTCGGC	5110	0.1482790059561152	No Hit
CCATCTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	4907	0.14238847010306407	No Hit
CCATCTGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGC	4749	0.13780371805980257	No Hit
CCATCTGGGGGGGCTGGTGAGGTGGCTCAGTGGGTAAGAGCACCCGACTG	4369	0.12677709922157876	No Hit
CCATCTGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCT	4321	0.1253842631578031	No Hit
CCATCTGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA	4311	0.12509408897784985	No Hit
CCATCTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	4170	0.1210026330405089	No Hit
CCATCTGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	4069	0.11807187382298098	No Hit
CCATCTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC	3917	0.11366122628769144	No Hit
CCATCTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	3909	0.11342908694372884	No Hit
CCATCTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCG	3785	0.10983092711230842	No Hit
CCATCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGC	3757	0.1090184394084393	No Hit
CCATCTGGGGCTCAGCCCGTAGCCCGTCGGTTCCGGAGTAAGTTCCAGGT	3492	0.10132882363967795	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	2.0312192596728113E-4	0.0	0.0	0.0	0.0
8	3.191915979485846E-4	0.0	0.0	0.0	0.0
9	3.7722643393923636E-4	0.0	0.0	0.0	0.0
10	0.0031338811434951943	0.0	0.0	0.0	0.0
11	0.006499901630952996	0.0	0.0	0.0	0.0
12	0.01027216597034536	0.0	0.0	0.0	0.0
13	0.014566743833653589	0.0	0.0	0.0	0.0
14	0.02475185755001297	0.0	0.0	0.0	0.0
15	0.03403743130851725	0.0	0.0	0.0	0.0
16	0.04697919973443259	0.0	0.0	0.0	0.0
17	0.05333401427540896	0.0	0.0	0.0	0.0
18	0.05945668947242272	0.0	0.0	0.0	0.0
19	0.07045429089265122	0.0	0.0	0.0	0.0
20	0.07834702858737987	0.0	0.0	0.0	0.0
21	0.08565941792220198	0.0	0.0	0.0	0.0
22	0.092072267299169	0.0	0.0	0.0	0.0
23	0.09836904700415472	0.0	0.0	0.0	0.0
24	0.10425958285720587	0.0	0.0	0.0	0.0
25	0.11038225805421963	0.0	0.0	0.0	0.0
26	0.11612770681729415	0.0	0.0	0.0	0.0
27	0.1237012529140742	0.0	0.0	0.0	0.0
28	0.13301584409057382	0.0	0.0	0.0	0.0
29	0.13887736252562963	0.0	0.0	0.0	0.0
30	0.14537726415658264	0.0	0.0	0.0	0.0
31	0.15144190451760572	0.0	0.0	0.0	0.0
32	0.15817394549252134	0.0	0.0	0.0	0.0
33	0.16400644650958185	0.0	0.0	0.0	0.0
34	0.17198623645829647	0.0	0.0	0.0	0.0
35	0.1871913634878472	0.0	0.0	0.0	0.0
36	0.20155498539553351	0.0	0.0	0.0	0.0
37	0.2121753603818228	0.0	0.0	0.0	0.0
38	0.21971988906060752	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAACGG	20	7.858674E-4	44.000004	42
CGATTAC	25	4.444699E-5	44.0	30
CGTACAA	25	4.444699E-5	44.0	22
TCGGTAC	35	1.4472062E-7	44.0	16
CTGGGAT	32015	0.0	40.893955	5
CCATCTG	367955	0.0	40.887337	1
CATCTGG	365485	0.0	40.880745	2
TCTGGGA	98855	0.0	40.85762	4
ATCTGGG	358635	0.0	40.837116	3
TCTGGGG	210185	0.0	40.453793	4
CTGGGGG	101245	0.0	40.360317	5
CTGGGGC	47405	0.0	40.08311	5
TGGGATC	10275	0.0	40.06034	6
TGGGGGG	68600	0.0	39.904667	6
TGGGGGC	17715	0.0	39.839684	6
TGGGATT	11590	0.0	39.691113	6
CCGATTT	100	0.0	39.6	41
TCTGGGC	32810	0.0	39.440414	4
TGGGACT	7030	0.0	39.399715	6
TCGAATA	95	0.0	39.36842	40
>>END_MODULE