FastQCFastQC Report
Fri 17 Jun 2016
SRR1527107_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527107_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3742302
Sequences flagged as poor quality0
Sequence length52
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC837462.2378204645162256No Hit
CCATCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT462621.2361909861897837No Hit
CCATCTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC266670.7125827899512118No Hit
CCATCTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT148050.39561211254463163No Hit
CCATCTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA121830.3255482855205165No Hit
CCATCTGGGGGGCTGATGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC101190.27039506699352434No Hit
CCATCTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC93500.24984621764892304No Hit
CCATCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC84410.22555635541973898No Hit
CCATCTGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT73700.1969376068526805No Hit
CCATCTGGGATTGTTAATTATTCTTTTGGATTCTTTTGAACTTGTAATACTA61730.16495194668949753No Hit
CCATCTGGGGGGGCTAGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT55310.14779673046162495No Hit
CCATCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCT51400.13734861590539726No Hit
CCATCTGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTC49430.13208447634637718No Hit
CCATCTGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGTCGGCTC48540.12970626101260668No Hit
CCATCTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG47430.1267401722255446No Hit
CCATCTGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT41480.11084086746606767No Hit
CCATCTGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA40670.10867642429713048No Hit
CCATCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT40390.10792822172021393No Hit
CCATCTGGGGGGGCTGGTGAGGTGGCTCAGTGGGTAAGAGCACCCGACTGCT40220.10747395586994314No Hit
CCATCTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC40080.10709985458148487No Hit
CCATCTGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTC39990.10685936089604742No Hit
CCATCTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT39720.10613787983973502No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTAAT206.313288E-446.00000419
CGATAAA253.418645E-546.034
TACGTAT253.418645E-546.037
ACGGTAC453.110472E-1045.99999637
CATTACG750.042.9333345
CATCTGG3957450.042.8726582
CCATCTG3987100.042.8520741
ATCTGGG3887150.042.79483
TCTGGGA1068150.042.774424
CTGGGAT349400.042.702065
TCGAATA650.042.4615440
TCTGGGG2296400.042.377334
CTGGGGG1110050.042.220715
CTAGTGA15900.042.0943414
TGGGGGG749600.041.7749446
TGGGATT125200.041.6461686
CGTATAC1050.041.61904511
TGGGATC112850.041.6180766
CTGGGGC533250.041.5099875
ACGTCTA508.731149E-1041.39999823
Produced by FastQC (version 0.11.5)