##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527107_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3742302
Sequences flagged as poor quality	0
Sequence length	52
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.585648886701286	34.0	31.0	34.0	31.0	34.0
2	32.97380729828859	34.0	31.0	34.0	31.0	34.0
3	32.94220482473087	34.0	31.0	34.0	31.0	34.0
4	36.44204529725287	37.0	37.0	37.0	35.0	37.0
5	36.38754301496779	37.0	37.0	37.0	35.0	37.0
6	36.41401148277183	37.0	37.0	37.0	35.0	37.0
7	36.545948456324474	37.0	37.0	37.0	35.0	37.0
8	36.63871034459539	37.0	37.0	37.0	35.0	37.0
9	38.72828168330616	39.0	39.0	39.0	39.0	39.0
10	38.174058106480985	39.0	39.0	39.0	37.0	39.0
11	37.94130430948651	39.0	38.0	39.0	35.0	39.0
12	37.14440630392737	39.0	35.0	39.0	35.0	39.0
13	37.024233747035915	39.0	35.0	39.0	34.0	39.0
14	37.69201951098548	40.0	36.0	40.0	34.0	40.0
15	37.574468068050095	40.0	35.0	40.0	33.0	40.0
16	32.54002322634571	39.0	34.0	40.0	3.0	40.0
17	34.02897307593027	39.0	33.0	40.0	17.0	40.0
18	36.055279076889036	39.0	34.0	40.0	30.0	40.0
19	36.97815328639965	39.0	35.0	40.0	32.0	40.0
20	37.30780118761126	39.0	35.0	40.0	33.0	40.0
21	37.54780560200646	40.0	35.0	40.0	33.0	40.0
22	37.6125053509845	40.0	35.0	40.0	34.0	40.0
23	37.614085661713034	40.0	35.0	40.0	34.0	40.0
24	37.60725270167934	40.0	35.0	40.0	34.0	40.0
25	37.5734686831795	40.0	35.0	40.0	34.0	40.0
26	37.48874489552153	40.0	35.0	40.0	34.0	40.0
27	37.398627101714396	40.0	35.0	40.0	34.0	40.0
28	37.306583755132536	40.0	35.0	40.0	34.0	40.0
29	37.099382412215796	39.0	35.0	40.0	33.0	40.0
30	37.071756368139184	39.0	35.0	40.0	33.0	40.0
31	37.05570314742103	39.0	35.0	40.0	33.0	40.0
32	36.9872530330262	39.0	35.0	40.0	33.0	40.0
33	36.64843029771515	39.0	35.0	40.0	32.0	40.0
34	36.297859445870486	39.0	35.0	40.0	30.0	40.0
35	36.33508947166744	39.0	35.0	40.0	31.0	40.0
36	36.47304573495138	39.0	35.0	40.0	31.0	40.0
37	36.377637614495036	38.0	35.0	40.0	31.0	40.0
38	36.39887133641272	38.0	35.0	40.0	31.0	40.0
39	36.051755309966964	38.0	35.0	40.0	31.0	40.0
40	36.0572190592849	38.0	35.0	40.0	30.0	40.0
41	36.05660660203265	38.0	35.0	40.0	31.0	40.0
42	36.02648957780532	38.0	35.0	40.0	31.0	40.0
43	35.98018839740887	38.0	35.0	40.0	31.0	40.0
44	36.227257981851814	38.0	35.0	40.0	31.0	40.0
45	36.266536479418285	38.0	35.0	40.0	32.0	40.0
46	36.154136945655374	38.0	35.0	40.0	31.0	40.0
47	35.85997629266692	38.0	35.0	40.0	31.0	40.0
48	35.843601077625486	37.0	35.0	40.0	31.0	40.0
49	35.808454796005236	37.0	35.0	40.0	31.0	40.0
50	35.35516588452776	37.0	35.0	40.0	30.0	40.0
51	35.47161559916864	36.0	35.0	40.0	30.0	40.0
52	35.18482821536049	36.0	34.0	40.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	5.0
15	25.0
16	62.0
17	176.0
18	405.0
19	818.0
20	1536.0
21	2751.0
22	4337.0
23	6583.0
24	9817.0
25	13918.0
26	19074.0
27	25862.0
28	34810.0
29	46499.0
30	62610.0
31	84230.0
32	118460.0
33	203986.0
34	322286.0
35	392855.0
36	331229.0
37	598045.0
38	891820.0
39	570103.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	0.0	100.0
2	0.0	0.0	0.0	100.0
3	0.0	100.0	0.0	0.0
4	0.0	0.0	100.0	0.0
5	0.0	0.0	0.0	100.0
6	0.0	0.0	100.0	0.0
7	99.25142332179499	0.5088846383856782	0.1844052136893281	0.055286826130013025
8	99.16177262016801	0.5265475635050298	0.18314930222093245	0.1285305141060235
9	97.7341219388494	1.1654323996299603	0.409774518464838	0.6906711430557982
10	57.88736451521016	27.239089736744926	5.7137558647057345	9.159789883339185
11	40.86025125711394	17.323481643116992	21.169296331509322	20.646970768259752
12	37.57887524844334	11.978242269063266	26.399713331526957	24.04316915096644
13	27.64635243227297	12.725135491470224	27.17950074579764	32.449011330459165
14	23.857828684055963	15.434350300964486	32.959499260081095	27.748321754898452
15	28.9123913569776	18.128681223482232	30.230376917736727	22.728550501803436
16	33.74463097847261	25.822020777585564	23.023449203190978	17.409899040750854
17	32.36224655305745	18.579847377362917	25.075849036234914	23.982057033344717
18	33.047760442636644	18.780873376868033	26.829769484130356	21.341596696364963
19	32.044073407223685	24.145405688797965	22.649267750170885	21.16125315380747
20	34.117823735230346	23.003862328588127	22.589571873141185	20.288742063040345
21	32.128406526250416	23.708802763646545	24.03940141656125	20.123389293541784
22	28.003405390585794	20.369387612223704	29.695011252432323	21.93219574475817
23	32.509989840477864	19.05963227981066	26.813148698314563	21.617229181396905
24	35.66198024638311	18.33817794501887	22.785173403963658	23.214668404634367
25	29.329647901211608	18.657019128867738	22.13354240251054	29.879790567410115
26	24.779587537296564	19.237330391828344	27.29445672743675	28.688625343438346
27	22.614022064493994	17.470289677316263	26.718126971046164	33.197561287143586
28	24.22492893411595	24.65832527679487	22.30570915976316	28.811036629326015
29	31.85475143374319	22.73955976829235	21.512079997819527	23.89360880014494
30	30.916799339016464	18.954429653192072	26.398457420058563	23.730313587732898
31	34.279969922256406	18.194122227441824	24.208842578712247	23.317065271589517
32	37.3186610808	17.214992269464087	24.6687733913511	20.797573258384812
33	35.91861373026549	18.06134299155974	25.516380024915147	20.503663253259624
34	28.092975927650947	20.26958273276716	31.75264850351468	19.884792836067213
35	24.96840180188558	28.18465746484383	28.792224678820684	18.05471605444991
36	25.531157025809247	32.40676460638399	23.234869874211114	18.827208493595656
37	31.124585883234435	28.703429065853047	19.714843965024738	20.457141085887777
38	30.226422132687315	28.461973405673834	20.26677697310372	21.04482748853513
39	31.6128415077137	25.290155631480303	19.987697411913842	23.109305448892155
40	27.88262411745498	22.985344314809442	20.14797309249761	28.984058475237966
41	22.98438234006769	26.476029994372446	20.788755156585438	29.750832508974423
42	21.88436422287672	24.17554756403946	21.847087701633914	32.0930005114499
43	22.854408863849045	20.241070870282517	24.099631724003036	32.80488854186541
44	24.62473632539544	19.138487487113547	23.930698270743516	32.3060779167475
45	28.462641443688934	21.424914397608745	22.49428293066674	27.618161228035575
46	25.897161693524467	25.407329499329556	21.95918448056838	26.7363243265776
47	23.819964289359866	22.517183273824507	25.586470573459863	28.076381863355763
48	25.92572699905032	21.78824691326355	27.932753690108385	24.353272397577747
49	29.471833112346356	20.037185668072752	24.357734891518643	26.13324632806225
50	24.74100166154415	20.482847188708984	26.075661451160276	28.70048969858659
51	23.671927065212802	20.69210341656018	28.203469415349165	27.432500102877853
52	22.52669613515959	20.91474712623407	27.421864937677398	29.13669180092895
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	1.0
7	1.5
8	2.0
9	51.0
10	100.0
11	465.5
12	831.0
13	2729.5
14	5929.0
15	7230.0
16	7201.5
17	7173.0
18	8125.5
19	9078.0
20	9868.0
21	10658.0
22	10902.0
23	11146.0
24	11340.5
25	11535.0
26	14065.0
27	16595.0
28	20537.5
29	24480.0
30	25157.0
31	25834.0
32	26022.0
33	26210.0
34	29559.5
35	32909.0
36	40967.5
37	49026.0
38	52535.5
39	58659.5
40	61274.0
41	69013.0
42	76752.0
43	79385.0
44	82018.0
45	93044.0
46	104070.0
47	111065.0
48	118060.0
49	134997.0
50	151934.0
51	159620.0
52	167306.0
53	185070.0
54	202834.0
55	227436.5
56	252039.0
57	296235.5
58	340432.0
59	361737.0
60	383042.0
61	393701.0
62	404360.0
63	427799.0
64	354340.5
65	257443.0
66	202772.5
67	148102.0
68	116516.5
69	84931.0
70	70428.0
71	55925.0
72	49326.0
73	42727.0
74	37668.5
75	32610.0
76	25305.0
77	18000.0
78	12840.5
79	7681.0
80	5819.5
81	3958.0
82	2649.0
83	1340.0
84	945.0
85	550.0
86	333.0
87	116.0
88	78.0
89	28.5
90	17.0
91	13.5
92	10.0
93	11.0
94	12.0
95	6.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	3742302.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	7.293486153177795
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.68026698603167	4.9362509010599815
2	10.608777690514978	1.54749946375825
3	4.730300996711164	1.03501154459628
4	2.5059905168184042	0.7310962853763958
5	1.469858573704769	0.5360196577222698
6	0.8842830602897919	0.3869703753427968
7	0.6014252432889089	0.30705406788694706
8	0.41464206980527896	0.2419348955719826
9	0.32757912789726734	0.21502744500498994
>10	4.3848112664331795	8.734967267300796
>50	3.178738155446381	16.894846491516695
>100	2.9737398932870174	38.72443216053065
>500	0.15185054774231574	7.480004597174235
>1k	0.08267418710414967	10.975029654000302
>5k	0.002530842462371929	1.2922603340666772
>10k+	0.002530842462371929	5.961594859090795
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCATCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	83746	2.2378204645162256	No Hit
CCATCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	46262	1.2361909861897837	No Hit
CCATCTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	26667	0.7125827899512118	No Hit
CCATCTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	14805	0.39561211254463163	No Hit
CCATCTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	12183	0.3255482855205165	No Hit
CCATCTGGGGGGCTGATGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	10119	0.27039506699352434	No Hit
CCATCTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC	9350	0.24984621764892304	No Hit
CCATCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	8441	0.22555635541973898	No Hit
CCATCTGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT	7370	0.1969376068526805	No Hit
CCATCTGGGATTGTTAATTATTCTTTTGGATTCTTTTGAACTTGTAATACTA	6173	0.16495194668949753	No Hit
CCATCTGGGGGGGCTAGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	5531	0.14779673046162495	No Hit
CCATCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCT	5140	0.13734861590539726	No Hit
CCATCTGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTC	4943	0.13208447634637718	No Hit
CCATCTGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGTCGGCTC	4854	0.12970626101260668	No Hit
CCATCTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	4743	0.1267401722255446	No Hit
CCATCTGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT	4148	0.11084086746606767	No Hit
CCATCTGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	4067	0.10867642429713048	No Hit
CCATCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT	4039	0.10792822172021393	No Hit
CCATCTGGGGGGGCTGGTGAGGTGGCTCAGTGGGTAAGAGCACCCGACTGCT	4022	0.10747395586994314	No Hit
CCATCTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	4008	0.10709985458148487	No Hit
CCATCTGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTC	3999	0.10685936089604742	No Hit
CCATCTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT	3972	0.10613787983973502	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	8.016456181248868E-5	0.0	0.0	0.0	0.0
8	1.0688608241665157E-4	0.0	0.0	0.0	0.0
9	2.1377216483330313E-4	0.0	0.0	0.0	0.0
10	0.0028057596634371035	0.0	0.0	0.0	0.0
11	0.005691683888686696	0.0	0.0	0.0	0.0
12	0.008764658758165428	0.0	0.0	0.0	0.0
13	0.01247895012214407	0.0	0.0	0.0	0.0
14	0.02225902666326769	0.0	0.0	0.0	0.0
15	0.030008267638474927	0.0	0.0	0.0	0.0
16	0.04251393928122316	0.0	0.0	0.0	0.0
17	0.04879349662320144	0.0	0.0	0.0	0.0
18	0.05493944636215891	0.0	0.0	0.0	0.0
19	0.06629609261892813	0.0	0.0	0.0	0.0
20	0.0750340298564894	0.0	0.0	0.0	0.0
21	0.08232900498142587	0.0	0.0	0.0	0.0
22	0.08855511928219582	0.0	0.0	0.0	0.0
23	0.09446057533571582	0.0	0.0	0.0	0.0
24	0.10060652507467328	0.0	0.0	0.0	0.0
25	0.10640509504577664	0.0	0.0	0.0	0.0
26	0.11193644981083836	0.0	0.0	0.0	0.0
27	0.11875043756489989	0.0	0.0	0.0	0.0
28	0.12804952673514858	0.0	0.0	0.0	0.0
29	0.13403514735048105	0.0	0.0	0.0	0.0
30	0.13996732492460523	0.0	0.0	0.0	0.0
31	0.14640721139020849	0.0	0.0	0.0	0.0
32	0.1519385661552702	0.0	0.0	0.0	0.0
33	0.15757680700274856	0.0	0.0	0.0	0.0
34	0.1659139214312474	0.0	0.0	0.0	0.0
35	0.17967550454239128	0.0	0.0	0.0	0.0
36	0.19351725221534766	0.0	0.0	0.0	0.0
37	0.20241551857653392	0.0	0.0	0.0	0.0
38	0.20979065826328286	0.0	0.0	0.0	0.0
39	0.21705891186761517	0.0	0.0	0.0	2.6721520604162892E-5
40	0.22392634266288503	0.0	0.0	0.0	2.6721520604162892E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTAAT	20	6.313288E-4	46.000004	19
CGATAAA	25	3.418645E-5	46.0	34
TACGTAT	25	3.418645E-5	46.0	37
ACGGTAC	45	3.110472E-10	45.999996	37
CATTACG	75	0.0	42.93333	45
CATCTGG	395745	0.0	42.872658	2
CCATCTG	398710	0.0	42.852074	1
ATCTGGG	388715	0.0	42.7948	3
TCTGGGA	106815	0.0	42.77442	4
CTGGGAT	34940	0.0	42.70206	5
TCGAATA	65	0.0	42.46154	40
TCTGGGG	229640	0.0	42.37733	4
CTGGGGG	111005	0.0	42.22071	5
CTAGTGA	1590	0.0	42.09434	14
TGGGGGG	74960	0.0	41.774944	6
TGGGATT	12520	0.0	41.646168	6
CGTATAC	105	0.0	41.619045	11
TGGGATC	11285	0.0	41.618076	6
CTGGGGC	53325	0.0	41.509987	5
ACGTCTA	50	8.731149E-10	41.399998	23
>>END_MODULE