FastQCFastQC Report
Fri 17 Jun 2016
SRR1527106_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527106_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3635303
Sequences flagged as poor quality0
Sequence length52
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC792522.18006587071284No Hit
CCATCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT453931.2486717063199408No Hit
CCATCTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC267290.735261957531463No Hit
CCATCTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT146670.4034601792477821No Hit
CCATCTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA116070.31928562763544055No Hit
CCATCTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC88110.24237319420141867No Hit
CCATCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC78760.2166531923198699No Hit
CCATCTGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT70860.19492185383171637No Hit
CCATCTGGGATTGTTAATTATTCTTTTGGATTCTTTTGAACTTGTAATACTA52160.14348185006861877No Hit
CCATCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCT51440.14150127238362248No Hit
CCATCTGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTC50320.13842037376251717No Hit
CCATCTGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGTCGGCTC47880.13170841605225206No Hit
CCATCTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG44480.1223556880953252No Hit
CCATCTGGGGGGGCTGGTGAGGTGGCTCAGTGGGTAAGAGCACCCGACTGCT41220.11338807246603652No Hit
CCATCTGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA40620.11173759106187298No Hit
CCATCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT40000.11003209361090396No Hit
CCATCTGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT39080.10750135545785316No Hit
CCATCTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT38780.10667611475577139No Hit
CCATCTGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTC38580.10612595428771687No Hit
CCATCTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC37290.10257741926876522No Hit
CCATCTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT37140.10216479891772433No Hit
CCATCTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC37070.10197224275390523No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAT253.4186247E-546.013
CCATCTG3900650.042.5470351
ATCTGGG3797900.042.540223
CATCTGG3875400.042.5393752
TCTGGGA1032850.042.4058654
CTGGGAT334700.042.26865
CTGGGGG1088900.042.200115
TCTGGGG2240000.042.1926774
TGGGGGG734600.041.8389636
CTGGGGC514650.041.723115
TGGGATT120400.041.472596
TCGAATA508.731149E-1041.440
TGGGATC109300.041.2653246
TGGGGGC191800.041.2513056
GAACCCG12850.041.16731639
TCTGGGC358250.041.0436864
TCTGGGT208500.041.0028764
CTGGGAC188450.040.898385
TGGGGCT346450.040.7885676
CTGGGGT282300.040.7530985
Produced by FastQC (version 0.11.5)