##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527106_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3635303
Sequences flagged as poor quality	0
Sequence length	52
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.55862083573226	34.0	31.0	34.0	31.0	34.0
2	32.969985170424586	34.0	31.0	34.0	31.0	34.0
3	32.91304026101813	34.0	31.0	34.0	31.0	34.0
4	36.43880991488192	37.0	37.0	37.0	35.0	37.0
5	36.40315869131129	37.0	37.0	37.0	35.0	37.0
6	36.408127740658756	37.0	37.0	37.0	35.0	37.0
7	36.54827451797003	37.0	37.0	37.0	35.0	37.0
8	36.6626289472982	37.0	37.0	37.0	35.0	37.0
9	38.676005823998715	39.0	39.0	39.0	39.0	39.0
10	38.17522528383466	39.0	39.0	39.0	37.0	39.0
11	37.88201368634196	39.0	38.0	39.0	35.0	39.0
12	37.11929679589294	39.0	35.0	39.0	35.0	39.0
13	36.936800041152004	39.0	35.0	39.0	34.0	39.0
14	37.625133860918886	40.0	36.0	40.0	33.0	40.0
15	37.685793178725405	40.0	36.0	40.0	33.0	40.0
16	37.74716963070203	40.0	35.0	40.0	34.0	40.0
17	37.548298174870155	40.0	35.0	40.0	33.0	40.0
18	37.56284661828739	40.0	35.0	40.0	33.0	40.0
19	37.55332636646794	40.0	35.0	40.0	33.0	40.0
20	37.5749688540405	40.0	35.0	40.0	33.0	40.0
21	33.38147356630245	39.0	33.0	40.0	10.0	40.0
22	34.14584946564289	39.0	34.0	40.0	17.0	40.0
23	36.20892976458909	39.0	34.0	40.0	30.0	40.0
24	37.023943258649965	39.0	35.0	40.0	32.0	40.0
25	37.31190302431462	39.0	35.0	40.0	33.0	40.0
26	37.393120463411165	39.0	35.0	40.0	34.0	40.0
27	37.38289490587167	40.0	35.0	40.0	34.0	40.0
28	37.299882568248094	40.0	35.0	40.0	34.0	40.0
29	37.26838560637174	39.0	35.0	40.0	34.0	40.0
30	37.16170701589386	39.0	35.0	40.0	33.0	40.0
31	37.04625254070981	39.0	35.0	40.0	33.0	40.0
32	37.007052782120226	39.0	35.0	40.0	33.0	40.0
33	36.69152530064206	39.0	35.0	40.0	32.0	40.0
34	36.3238277524597	39.0	35.0	40.0	30.0	40.0
35	36.358065063627436	39.0	35.0	40.0	31.0	40.0
36	36.42519509377898	39.0	35.0	40.0	31.0	40.0
37	36.542306102132336	38.0	35.0	40.0	32.0	40.0
38	36.49856256823709	38.0	35.0	40.0	32.0	40.0
39	36.36707751733487	38.0	35.0	40.0	31.0	40.0
40	31.50473784441077	36.0	31.0	40.0	9.0	40.0
41	32.43401774212494	36.0	31.0	40.0	15.0	40.0
42	34.30635218027218	36.0	32.0	40.0	24.0	40.0
43	35.2566891948209	37.0	34.0	40.0	29.0	40.0
44	35.95734908479431	38.0	35.0	40.0	31.0	40.0
45	36.17281393050318	38.0	35.0	40.0	31.0	40.0
46	36.14488778514473	38.0	35.0	40.0	31.0	40.0
47	36.09648659272693	38.0	35.0	40.0	31.0	40.0
48	35.97275000185679	37.0	35.0	40.0	31.0	40.0
49	35.87156338825127	37.0	35.0	40.0	31.0	40.0
50	35.80860577508945	37.0	35.0	40.0	31.0	40.0
51	35.67399278684611	37.0	35.0	40.0	31.0	40.0
52	35.325094496937396	36.0	34.0	40.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	11.0
15	21.0
16	64.0
17	186.0
18	431.0
19	910.0
20	1635.0
21	2831.0
22	4677.0
23	6987.0
24	10168.0
25	14392.0
26	19837.0
27	27004.0
28	36354.0
29	48997.0
30	67008.0
31	95806.0
32	158809.0
33	154261.0
34	331016.0
35	445374.0
36	411429.0
37	370601.0
38	843495.0
39	582998.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	0.0	100.0
2	0.0	0.0	0.0	100.0
3	0.0	100.0	0.0	0.0
4	0.0	0.0	100.0	0.0
5	0.0	0.0	0.0	100.0
6	0.0	0.0	100.0	0.0
7	99.2582186409221	0.5031492560592611	0.18270829144090603	0.05592381157774194
8	99.18691784426223	0.5179485726499277	0.16702871810135222	0.12810486498649493
9	97.67879046120778	1.1717867809093219	0.45443254661303334	0.6949902112698721
10	57.881282523079925	27.259708475469584	5.70940028932939	9.149608712121108
11	40.94800350892346	17.29621987493202	21.18467153907116	20.571105077073355
12	37.588943755169794	11.992948043120478	26.434110169083567	23.983998032626168
13	27.75710855463767	12.730878278922003	27.11787710680513	32.394136059635194
14	23.82005021314592	15.465093281082758	32.94459911594714	27.770257389824177
15	28.886505471483392	18.027658217210504	30.27491793668918	22.810918374616918
16	36.23233056501755	17.418245466746512	26.242599310153793	20.106824658082147
17	32.35496463430971	18.584778215185914	25.052767265892278	24.00748988461209
18	33.08978646346673	18.791225930823373	26.822220871272627	21.296766734437266
19	32.04494921056099	24.161782387877984	22.637947923460576	21.15532047810045
20	34.161691611400755	22.97838722109271	22.595448027303362	20.26447314020317
21	36.24558943229766	22.518645625962954	22.92741485372746	18.30835008801192
22	28.188104265311587	20.31979177526605	29.586722207199784	21.90538175222258
23	32.49093679398939	19.110896670786452	26.79652837741448	21.60163815780968
24	35.72612791836059	18.336958432350755	22.77625826512948	23.160655384159174
25	29.314860411910644	18.67382168694054	22.14090544859672	29.8704124525521
26	24.79091839112173	19.213171501797788	27.316540051819615	28.67937005526087
27	22.569507961234596	17.465696807116217	26.691007599641626	33.27378763200757
28	24.190748336521054	24.633022336790084	22.344354789683283	28.831874537005582
29	31.71276231994967	22.752683889073346	21.5524263039422	23.98212748703478
30	30.896681789661002	18.952587996103766	26.38583358801178	23.764896626223454
31	34.33986107898021	18.184453950606045	24.221337258544885	23.25434771186886
32	37.27240892987462	17.271517669916374	24.692687239550594	20.76338616065841
33	35.950263293046	18.141899038402027	25.41999937831867	20.487838290233302
34	28.13751701027397	20.25649581341638	31.74695479303926	19.85903238327039
35	25.02019776618345	28.186976436352072	28.70613536203172	18.086690435432757
36	25.611565253295254	32.35823808909464	23.2042831092759	18.82591354833421
37	31.000277005795667	28.733643385434448	19.713624971563583	20.552454637206306
38	30.23013487458955	28.40987394998436	20.30947626649003	21.050514908936062
39	31.42629376423368	25.326389574679194	19.948873587703694	23.298443073383428
40	28.327624960010212	27.4445073766891	18.733073969350013	25.494793693950683
41	23.044791589586893	26.45240300464638	20.83432935301404	29.668476052752684
42	21.84915535238741	24.20381464763735	21.864944957820573	32.08208504215467
43	22.878615620211026	20.312887261391964	24.045120860627023	32.763376257769984
44	24.6008654574323	19.19784953276247	23.900593705669102	32.30069130413614
45	28.444699107612216	21.428750230723544	22.477273558765255	27.64927710289899
46	25.881473978922802	25.39416934434351	21.96895279430628	26.75540388242741
47	23.608651053295972	22.534930375817368	25.597316097172644	28.25910247371402
48	25.906753852429908	21.797440268390282	27.93260974394707	24.363196135232744
49	29.47374675508479	20.068671029622564	24.368917804100512	26.088664411192138
50	24.734059306748296	20.379896806401007	26.173141551061907	28.712902335788794
51	23.755268818032498	20.686748807458414	28.16637292682343	27.391609447685656
52	22.506074459267907	20.945434259537652	27.411992893027076	29.13649838816737
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	1.0
7	0.5
8	0.0
9	59.0
10	118.0
11	418.5
12	719.0
13	2438.0
14	5432.0
15	6707.0
16	6749.5
17	6792.0
18	7842.0
19	8892.0
20	9387.0
21	9882.0
22	10427.5
23	10973.0
24	11197.5
25	11422.0
26	13681.5
27	15941.0
28	19197.0
29	22453.0
30	23546.5
31	24640.0
32	24953.0
33	25266.0
34	28446.5
35	31627.0
36	39291.0
37	46955.0
38	50298.0
39	56727.0
40	59813.0
41	66836.0
42	73859.0
43	76841.5
44	79824.0
45	90505.0
46	101186.0
47	107978.5
48	114771.0
49	130527.0
50	146283.0
51	154672.5
52	163062.0
53	180477.5
54	197893.0
55	218814.5
56	239736.0
57	282838.5
58	325941.0
59	349005.5
60	372070.0
61	378352.0
62	384634.0
63	416477.5
64	351788.5
65	255256.0
66	201232.0
67	147208.0
68	116175.0
69	85142.0
70	69841.5
71	54541.0
72	48152.5
73	41764.0
74	36784.5
75	31805.0
76	24924.5
77	18044.0
78	12911.5
79	7779.0
80	5909.0
81	4039.0
82	2738.0
83	1437.0
84	993.5
85	550.0
86	320.5
87	91.0
88	68.0
89	26.5
90	8.0
91	7.5
92	7.0
93	7.5
94	8.0
95	4.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	3635303.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	7.314867202444949
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.49203898125322	4.936953023701053
2	10.610198775285305	1.552243900655121
3	4.705700770925705	1.0326472870129304
4	2.478351508563421	0.7251524866448213
5	1.4152225190775953	0.5176082394481112
6	0.8812774000691979	0.3867856290013279
7	0.5546317596603993	0.283994036772193
8	0.41351024317290136	0.24198180125283936
9	0.30859930043623474	0.2031626611312622
>10	4.645932481288411	9.354523246536164
>50	3.314653461760264	17.579014413378797
>100	2.943373510031095	38.14638067401676
>500	0.1501856003173131	7.410989009599294
>1k	0.08147899137449538	10.643575327541631
>5k	0.0026425618824160663	1.2617650479097025
>10k+	0.0022021349020133886	5.723223215398003
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCATCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	79252	2.18006587071284	No Hit
CCATCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	45393	1.2486717063199408	No Hit
CCATCTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	26729	0.735261957531463	No Hit
CCATCTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	14667	0.4034601792477821	No Hit
CCATCTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	11607	0.31928562763544055	No Hit
CCATCTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC	8811	0.24237319420141867	No Hit
CCATCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	7876	0.2166531923198699	No Hit
CCATCTGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT	7086	0.19492185383171637	No Hit
CCATCTGGGATTGTTAATTATTCTTTTGGATTCTTTTGAACTTGTAATACTA	5216	0.14348185006861877	No Hit
CCATCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCT	5144	0.14150127238362248	No Hit
CCATCTGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTC	5032	0.13842037376251717	No Hit
CCATCTGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGTCGGCTC	4788	0.13170841605225206	No Hit
CCATCTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	4448	0.1223556880953252	No Hit
CCATCTGGGGGGGCTGGTGAGGTGGCTCAGTGGGTAAGAGCACCCGACTGCT	4122	0.11338807246603652	No Hit
CCATCTGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	4062	0.11173759106187298	No Hit
CCATCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT	4000	0.11003209361090396	No Hit
CCATCTGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT	3908	0.10750135545785316	No Hit
CCATCTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	3878	0.10667611475577139	No Hit
CCATCTGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTC	3858	0.10612595428771687	No Hit
CCATCTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	3729	0.10257741926876522	No Hit
CCATCTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT	3714	0.10216479891772433	No Hit
CCATCTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC	3707	0.10197224275390523	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	5.501604680545198E-5	0.0	0.0	0.0	0.0
8	1.3754011701362995E-4	0.0	0.0	0.0	0.0
9	1.9255616381908192E-4	0.0	0.0	0.0	0.0
10	0.003135914667910763	0.0	0.0	0.0	0.0
11	0.006436877476237881	0.0	0.0	0.0	0.0
12	0.009710332261162274	0.0	0.0	0.0	0.0
13	0.013588963560946639	0.0	0.0	0.0	0.0
14	0.023574376056136173	0.0	0.0	0.0	0.0
15	0.03234943552160576	0.0	0.0	0.0	0.0
16	0.044507981865610655	0.0	0.0	0.0	0.0
17	0.050449714920599464	0.0	0.0	0.0	0.0
18	0.05584128750753376	0.0	0.0	0.0	0.0
19	0.06574417593251512	0.0	0.0	0.0	0.0
20	0.07385904283631928	0.0	0.0	0.0	0.0
21	0.08032342833595989	0.0	0.0	0.0	0.0
22	0.0868978459292114	0.0	0.0	0.0	0.0
23	0.09264702282038113	0.0	2.750802340272599E-5	0.0	0.0
24	0.0994965206476599	0.0	2.750802340272599E-5	0.0	0.0
25	0.10571333393667598	0.0	2.750802340272599E-5	0.0	0.0
26	0.11179260710867843	0.0	2.750802340272599E-5	0.0	0.0
27	0.11842204074873539	0.0	2.750802340272599E-5	0.0	0.0
28	0.1287100415013549	0.0	2.750802340272599E-5	0.0	0.0
29	0.13423915420530283	0.0	2.750802340272599E-5	0.0	0.0
30	0.13924561446459896	0.0	2.750802340272599E-5	0.0	0.0
31	0.14551744380042048	0.0	2.750802340272599E-5	0.0	0.0
32	0.15151419290221477	0.0	2.750802340272599E-5	0.0	0.0
33	0.15663068525512178	0.0	2.750802340272599E-5	0.0	0.0
34	0.1644704719248987	0.0	2.750802340272599E-5	0.0	0.0
35	0.17701413059654175	0.0	2.750802340272599E-5	0.0	0.0
36	0.1901904738064475	0.0	2.750802340272599E-5	0.0	0.0
37	0.19915808943573618	0.0	2.750802340272599E-5	0.0	0.0
38	0.2063651915672504	0.0	2.750802340272599E-5	0.0	0.0
39	0.2130496412541128	0.0	2.750802340272599E-5	0.0	0.0
40	0.22017421931541883	0.0	2.750802340272599E-5	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGAAT	25	3.4186247E-5	46.0	13
CCATCTG	390065	0.0	42.547035	1
ATCTGGG	379790	0.0	42.54022	3
CATCTGG	387540	0.0	42.539375	2
TCTGGGA	103285	0.0	42.405865	4
CTGGGAT	33470	0.0	42.2686	5
CTGGGGG	108890	0.0	42.20011	5
TCTGGGG	224000	0.0	42.192677	4
TGGGGGG	73460	0.0	41.838963	6
CTGGGGC	51465	0.0	41.72311	5
TGGGATT	12040	0.0	41.47259	6
TCGAATA	50	8.731149E-10	41.4	40
TGGGATC	10930	0.0	41.265324	6
TGGGGGC	19180	0.0	41.251305	6
GAACCCG	1285	0.0	41.167316	39
TCTGGGC	35825	0.0	41.043686	4
TCTGGGT	20850	0.0	41.002876	4
CTGGGAC	18845	0.0	40.89838	5
TGGGGCT	34645	0.0	40.788567	6
CTGGGGT	28230	0.0	40.753098	5
>>END_MODULE