FastQCFastQC Report
Fri 17 Jun 2016
SRR1527105_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527105_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2007
Sequences flagged as poor quality0
Sequence length50
%GC46

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGCCTTATGGTTACAGTATGCCCATCGCAGTTCGCTACACGCAGGAC1778.819133034379671No Hit
CGTGGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1638.121574489287493No Hit
CGTGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1597.922272047832586No Hit
CGTGGCCTTGCTATTGACTCTACTGTAGACATTTTTACTTTTTATGTCCC1376.826108619830593No Hit
CGTGGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA894.434479322371699No Hit
CGTGGCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA582.8898854010961634No Hit
CGTGGCGGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT562.7902341803687096No Hit
CGTGGCGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA502.4912805181863478No Hit
CGTGGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA502.4912805181863478No Hit
CGTGGCGAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA391.9431988041853512No Hit
CGTGGCGGCAGTCCAGTGCCCGTGGCGGGGAGCTGTGGGATCAAACCCCC251.2456402590931739No Hit
CGTGGCGGCAGTCCAGTGCCCGTGGCGGGGAGCTGTGGGATCAACCCCCC251.2456402590931739No Hit
CGTGGCGGGGGGGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA190.9466865969108121No Hit
CGTGGCGGGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180.8968609865470852No Hit
CGTGGCGGGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170.8470353761833582No Hit
CGTGGCGGGACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT170.8470353761833582No Hit
CGTGGCGGCGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150.7473841554559043No Hit
CGTGGCGGCAGTCCAGTGCCCGTGGCGGGGAGCTGTGGGATCAACCCCTC150.7473841554559043No Hit
CGTGGCGGGGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGG150.7473841554559043No Hit
CGTGGCGGGGGGGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA120.5979073243647235No Hit
CGTGGCGAGCTCTGTTTTGTGATTGAAGCCGATCAGATTACCTTTGAAAC90.4484304932735426No Hit
CGTGGCGGGGGGTTGCTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.4484304932735426No Hit
CGTGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGGGGGGGGG80.3986048829098156No Hit
CGTGGCGGCAGTCCAGTGCCCGTGGCGGGGAGCTGTGGGATCAAACCCTC70.3487792725460887No Hit
CGTGGCGGGGGCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70.3487792725460887No Hit
CGTGGCGGGAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.3487792725460887No Hit
CGTGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGGGGGGGGGG70.3487792725460887No Hit
CGTGGCGGGGGGGGGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGG70.3487792725460887No Hit
CGTGGCGAGAGAAGTCCTGGGTTCAAATCCCAGCAACCACATGGTGAGTC60.29895366218236175No Hit
CGTGGCGGGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.2491280518186348No Hit
CGTGGCACGGGCCATGGCAATAAAGGGTTGTTCGAGGGTGTCTTCCAGGG50.2491280518186348No Hit
CGTGGCGGCAGTCCAGTGCCCGTGGCGGGGAGCTGTGGGATTAAACCCCC50.2491280518186348No Hit
CGTGGCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.2491280518186348No Hit
CGTGGCGGGGGGGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGG40.1993024414549078No Hit
CGTGGCGGGGGGGGGGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGG40.1993024414549078No Hit
CGTGGCGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA40.1993024414549078Illumina Single End Adapter 2 (97% over 34bp)
CGTGGCGGCAGTCCAGTGCCCGTGGCGGGGAGGTGTGGGATCAAACCCCC40.1993024414549078No Hit
CGTGGCGGGGGGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.1993024414549078No Hit
CGTGGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.1993024414549078No Hit
CGTGGCGGCAGTCCAGTGCCCGTGGCGGGGAGCTGTGGGATTAACCCCCC40.1993024414549078No Hit
CGTGGCGGGGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGCGGGGGGGGG40.1993024414549078No Hit
CGTGGCGGCAGTCCAGTGCCCGTGGCGGGGAGGTGTGGGATCAACCCCCC30.14947683109118087No Hit
CGTGGCGGGGGGGGGGGGGGGGGGGCGGCGGGGGGGGGGGGGGGGGGGGG30.14947683109118087No Hit
CGTGGCGAGAAGCAGTGGTAGAGACGGGGCTTCACTATGTTGGCCAGGCT30.14947683109118087No Hit
CGTGGCGGCAGTCCAGTGCCCGTGGCGGGGAGCTGGGGGATCAACCCCCC30.14947683109118087No Hit
CGTGGCGGCAGTCCAGTGCCCGTGGCGGGGAGCTGTGGTATCAACCCCCC30.14947683109118087No Hit
CGTGGCGGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAAAAAA30.14947683109118087No Hit
CGTGGCGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGGATCGGAAGAG30.14947683109118087Illumina Single End Adapter 2 (97% over 34bp)
CGTGGCGAGGCTAAGCAATGCACTCTGATACCTGTTTCGGTCAGTTGTTC30.14947683109118087No Hit
CGTGGCGGGGGGCTCGTGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA30.14947683109118087No Hit
CGTGGCGGCAGTCCAGTGCCCGTGGCGGGAAGCTGTGGGATCAAACCCCC30.14947683109118087No Hit
CGTGGCGGGGCTCATCGTCTCTTTCCGCTGCCATGAAAGGAGTGCTTTTT30.14947683109118087No Hit
CGTGGCCTTGCTATTGACTCTACTGTAGACATTTTTACTTTTTATGTCGC30.14947683109118087No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGACA206.117847E-444.00000425
ACTGTAG206.117847E-444.00000422
GCCTTGC206.117847E-444.0000045
TTACTTT206.117847E-444.00000435
TGACTCT206.117847E-444.00000415
TATGTCC206.117847E-444.00000443
TTTTTAT206.117847E-444.00000439
TTTTTAC206.117847E-444.00000432
CATTTTT206.117847E-444.00000430
CCTTGCT206.117847E-444.0000046
ACTCTAC206.117847E-444.00000417
TCTACTG206.117847E-444.00000419
ACATTTT206.117847E-444.00000429
TATTGAC206.117847E-444.00000412
TTTTATG206.117847E-444.00000440
GCTATTG206.117847E-444.00000410
CTCTACT206.117847E-444.00000418
GACATTT206.117847E-444.00000428
TTTTACT206.117847E-444.00000433
GGCCTTG206.117847E-444.0000044
Produced by FastQC (version 0.11.5)