##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527105_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2007
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.409068261086198	31.0	30.0	31.0	28.0	33.0
2	30.82311908320877	31.0	30.0	31.0	30.0	34.0
3	29.94369706028899	31.0	30.0	31.0	28.0	33.0
4	34.970104633781766	35.0	35.0	35.0	33.0	37.0
5	35.27703039362232	35.0	35.0	37.0	35.0	37.0
6	35.01993024414549	35.0	35.0	37.0	35.0	37.0
7	35.482311908320874	35.0	35.0	37.0	35.0	37.0
8	35.36173393124066	35.0	35.0	37.0	35.0	37.0
9	35.85002491280518	37.0	35.0	39.0	31.0	39.0
10	30.135525660189337	35.0	23.0	39.0	9.0	39.0
11	26.920279023418036	28.0	20.0	37.0	12.0	39.0
12	27.241654210264077	28.0	20.0	37.0	13.0	39.0
13	29.002491280518186	32.0	22.0	37.0	15.0	39.0
14	30.258594917787743	34.0	22.0	39.0	15.0	40.0
15	31.105132037867463	35.0	24.0	40.0	16.0	40.0
16	31.858495266567015	35.0	26.0	40.0	18.0	40.0
17	31.705530642750375	34.0	27.0	39.0	18.0	40.0
18	31.39810662680618	34.0	27.0	39.0	15.0	40.0
19	31.10911808669656	33.0	25.0	39.0	18.0	40.0
20	31.491280518186347	33.0	28.0	37.0	18.0	40.0
21	31.19880418535127	33.0	26.0	38.0	15.0	40.0
22	30.426008968609864	33.0	25.0	36.0	15.0	40.0
23	30.05281514698555	33.0	25.0	35.0	15.0	40.0
24	30.192825112107624	33.0	25.0	35.0	15.0	40.0
25	30.225710014947683	33.0	25.0	37.0	15.0	40.0
26	29.13153961136024	33.0	25.0	35.0	6.0	40.0
27	29.288490284005977	33.0	25.0	35.0	9.0	40.0
28	29.689088191330345	33.0	25.0	37.0	9.0	40.0
29	28.997508719481814	33.0	25.0	35.0	9.0	40.0
30	27.244145490782262	33.0	18.0	35.0	7.0	40.0
31	27.57498754359741	33.0	18.0	35.0	8.0	40.0
32	26.575984055804685	33.0	15.0	35.0	7.0	40.0
33	26.69706028898854	33.0	15.0	35.0	6.0	40.0
34	27.04534130543099	33.0	15.0	35.0	9.0	40.0
35	27.216243148978574	33.0	18.0	35.0	9.0	40.0
36	27.10911808669656	33.0	18.0	35.0	9.0	40.0
37	27.33582461385152	33.0	18.0	35.0	9.0	40.0
38	28.022421524663677	33.0	21.0	35.0	10.0	40.0
39	26.132536123567515	33.0	15.0	35.0	6.0	40.0
40	25.61484803188839	33.0	11.0	35.0	8.0	40.0
41	25.338315894369707	33.0	10.0	35.0	6.0	40.0
42	25.27902341803687	33.0	9.0	35.0	8.0	40.0
43	25.64623816641754	33.0	10.0	35.0	8.0	40.0
44	26.900348779272544	33.0	18.0	35.0	9.0	40.0
45	25.97608370702541	33.0	15.0	35.0	8.0	40.0
46	26.360737419033384	33.0	17.0	35.0	8.0	40.0
47	27.29197807673144	33.0	20.0	35.0	9.0	40.0
48	27.1604384653712	33.0	20.0	35.0	8.0	40.0
49	25.699551569506728	31.0	15.0	35.0	8.0	40.0
50	25.886397608370704	31.0	15.0	35.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	2.0
14	16.0
15	41.0
16	59.0
17	65.0
18	48.0
19	44.0
20	42.0
21	53.0
22	39.0
23	69.0
24	88.0
25	117.0
26	82.0
27	71.0
28	53.0
29	51.0
30	71.0
31	125.0
32	217.0
33	91.0
34	109.0
35	59.0
36	65.0
37	44.0
38	114.0
39	171.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	0.0	100.0
2	100.0	0.0	0.0	0.0
3	0.0	0.0	100.0	0.0
4	100.0	0.0	0.0	0.0
5	100.0	0.0	0.0	0.0
6	0.0	0.0	0.0	100.0
7	81.21574489287494	1.345291479820628	0.04982561036372695	17.38913801694071
8	75.88440458395615	6.527154957648232	17.289486796213254	0.29895366218236175
9	67.71300448430493	3.9362232187344297	18.883906327852515	9.466865969108122
10	64.77329347284504	28.94867962132536	1.4947683109118086	4.783258594917788
11	50.32386646736422	18.883906327852515	18.385650224215247	12.406576980568012
12	42.99950174389636	29.048330842052817	23.966118584952667	3.9860488290981566
13	35.22670652715495	40.109616342800194	10.563029397110114	14.100647732934728
14	20.777279521674142	43.04932735426009	25.26158445440957	10.911808669656203
15	23.766816143497756	45.73991031390135	26.307922272047833	4.185351270553064
16	36.12356751370204	47.633283507722965	12.157448928749377	4.08570004982561
17	23.218734429496763	44.94270054808172	17.937219730941703	13.901345291479823
18	29.99501743896363	46.238166417538615	12.007972097658197	11.758844045839561
19	29.9451918285999	37.86746387643249	18.784255107125063	13.403089187842552
20	20.528151469855505	40.00996512207274	20.179372197309416	19.282511210762333
21	21.325361235675135	50.17438963627304	17.83756851021425	10.662680617837568
22	26.258096661684107	48.28101644245142	21.026407573492776	4.434479322371699
23	28.749377179870457	41.205779770802195	20.677628300946687	9.367214748380666
24	26.806178375685104	41.803687095166914	19.681116093672145	11.709018435475835
25	35.176880916791234	41.30543099152965	11.310413552566018	12.207274539113104
26	18.33582461385152	41.45490782262083	17.887394120577977	22.321873442949677
27	27.60338814150473	58.594917787742894	10.612855007473842	3.188839063278525
28	34.97757847533632	41.75386148480319	20.57797708021923	2.690582959641256
29	26.307922272047833	48.67962132536123	12.057797708021925	12.954658694569007
30	35.02740408570005	43.09915296462382	10.81215744892875	11.061285500747383
31	17.937219730941703	56.502242152466366	11.509715994020926	14.050822122571002
32	24.763328350772294	51.32037867463877	19.880418535127056	4.0358744394618835
33	29.14798206278027	41.85351270553064	19.48181365221724	9.51669157947185
34	18.734429496761333	41.4050822122571	35.72496263079223	4.135525660189337
35	27.404085700049823	41.9531639262581	27.50373692077728	3.1390134529147984
36	20.42850024912805	41.15595416043846	26.15844544095665	12.25710014947683
37	36.57199800697558	48.729446935724965	11.160936721474839	3.537618335824614
38	27.60338814150473	39.511709018435475	12.406576980568012	20.47832585949178
39	28.300946686596912	39.36223218734429	29.546586945690084	2.7902341803687096
40	20.229197807673145	56.95067264573991	18.236173393124066	4.58395615346288
41	19.48181365221724	40.50822122571002	25.809666168410562	14.200298953662182
42	21.574489287493773	48.92874937717987	19.681116093672145	9.81564524165421
43	20.62780269058296	47.43398106626806	18.98355754857997	12.954658694569007
44	30.642750373692078	54.26008968609865	11.858495266567015	3.2386646736422517
45	20.12954658694569	43.04932735426009	19.282511210762333	17.538614848031887
46	29.546586945690084	47.08520179372198	11.260587942202292	12.10762331838565
47	30.194319880418536	39.56153462879921	18.63477827603388	11.609367214748382
48	29.895366218236173	38.714499252615845	12.207274539113104	19.182859990034878
49	20.92675635276532	47.53363228699551	14.848031888390633	16.69157947184853
50	20.62780269058296	36.87095166915795	12.556053811659194	29.9451918285999
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	2.0
12	4.0
13	4.0
14	4.0
15	48.0
16	92.0
17	140.5
18	189.0
19	195.5
20	202.0
21	144.0
22	86.0
23	81.0
24	76.0
25	56.5
26	37.0
27	34.5
28	32.0
29	43.5
30	55.0
31	45.0
32	35.0
33	37.0
34	39.0
35	48.5
36	58.0
37	38.0
38	18.0
39	92.0
40	166.0
41	93.5
42	21.0
43	17.5
44	14.0
45	22.5
46	31.0
47	26.0
48	21.0
49	16.5
50	12.0
51	13.0
52	14.0
53	16.0
54	18.0
55	111.5
56	205.0
57	120.0
58	35.0
59	27.5
60	20.0
61	22.0
62	24.0
63	17.0
64	10.0
65	11.0
66	12.0
67	13.5
68	15.0
69	25.0
70	35.0
71	50.0
72	65.0
73	53.0
74	41.0
75	24.5
76	8.0
77	7.0
78	6.0
79	4.5
80	3.0
81	1.5
82	0.0
83	0.5
84	1.0
85	2.0
86	3.0
87	3.0
88	3.0
89	3.5
90	4.0
91	2.5
92	1.0
93	1.5
94	2.0
95	9.0
96	16.0
97	145.0
98	274.0
99	137.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2007.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.97757847533632
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.75498575498575	29.99501743896363
2	6.695156695156696	4.683607374190333
3	1.7094017094017095	1.7937219730941705
4	1.1396011396011396	1.5944195316392624
5	0.5698005698005698	0.9965122072745392
6	0.14245014245014245	0.29895366218236175
7	0.7122507122507122	1.7438963627304436
8	0.14245014245014245	0.3986048829098156
9	0.2849002849002849	0.8968609865470852
>10	1.8518518518518516	15.794718485301445
>50	0.4273504273504274	10.114598903836573
>100	0.5698005698005698	31.689088191330345
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTGGCCTTATGGTTACAGTATGCCCATCGCAGTTCGCTACACGCAGGAC	177	8.819133034379671	No Hit
CGTGGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	163	8.121574489287493	No Hit
CGTGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	159	7.922272047832586	No Hit
CGTGGCCTTGCTATTGACTCTACTGTAGACATTTTTACTTTTTATGTCCC	137	6.826108619830593	No Hit
CGTGGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	89	4.434479322371699	No Hit
CGTGGCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	58	2.8898854010961634	No Hit
CGTGGCGGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	56	2.7902341803687096	No Hit
CGTGGCGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	50	2.4912805181863478	No Hit
CGTGGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	50	2.4912805181863478	No Hit
CGTGGCGAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	39	1.9431988041853512	No Hit
CGTGGCGGCAGTCCAGTGCCCGTGGCGGGGAGCTGTGGGATCAAACCCCC	25	1.2456402590931739	No Hit
CGTGGCGGCAGTCCAGTGCCCGTGGCGGGGAGCTGTGGGATCAACCCCCC	25	1.2456402590931739	No Hit
CGTGGCGGGGGGGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	19	0.9466865969108121	No Hit
CGTGGCGGGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18	0.8968609865470852	No Hit
CGTGGCGGGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17	0.8470353761833582	No Hit
CGTGGCGGGACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17	0.8470353761833582	No Hit
CGTGGCGGCGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15	0.7473841554559043	No Hit
CGTGGCGGCAGTCCAGTGCCCGTGGCGGGGAGCTGTGGGATCAACCCCTC	15	0.7473841554559043	No Hit
CGTGGCGGGGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGG	15	0.7473841554559043	No Hit
CGTGGCGGGGGGGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	12	0.5979073243647235	No Hit
CGTGGCGAGCTCTGTTTTGTGATTGAAGCCGATCAGATTACCTTTGAAAC	9	0.4484304932735426	No Hit
CGTGGCGGGGGGTTGCTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	9	0.4484304932735426	No Hit
CGTGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGGGGGGGGG	8	0.3986048829098156	No Hit
CGTGGCGGCAGTCCAGTGCCCGTGGCGGGGAGCTGTGGGATCAAACCCTC	7	0.3487792725460887	No Hit
CGTGGCGGGGGCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7	0.3487792725460887	No Hit
CGTGGCGGGAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7	0.3487792725460887	No Hit
CGTGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGGGGGGGGGG	7	0.3487792725460887	No Hit
CGTGGCGGGGGGGGGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGG	7	0.3487792725460887	No Hit
CGTGGCGAGAGAAGTCCTGGGTTCAAATCCCAGCAACCACATGGTGAGTC	6	0.29895366218236175	No Hit
CGTGGCGGGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5	0.2491280518186348	No Hit
CGTGGCACGGGCCATGGCAATAAAGGGTTGTTCGAGGGTGTCTTCCAGGG	5	0.2491280518186348	No Hit
CGTGGCGGCAGTCCAGTGCCCGTGGCGGGGAGCTGTGGGATTAAACCCCC	5	0.2491280518186348	No Hit
CGTGGCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5	0.2491280518186348	No Hit
CGTGGCGGGGGGGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGG	4	0.1993024414549078	No Hit
CGTGGCGGGGGGGGGGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGG	4	0.1993024414549078	No Hit
CGTGGCGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA	4	0.1993024414549078	Illumina Single End Adapter 2 (97% over 34bp)
CGTGGCGGCAGTCCAGTGCCCGTGGCGGGGAGGTGTGGGATCAAACCCCC	4	0.1993024414549078	No Hit
CGTGGCGGGGGGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4	0.1993024414549078	No Hit
CGTGGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4	0.1993024414549078	No Hit
CGTGGCGGCAGTCCAGTGCCCGTGGCGGGGAGCTGTGGGATTAACCCCCC	4	0.1993024414549078	No Hit
CGTGGCGGGGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGCGGGGGGGGG	4	0.1993024414549078	No Hit
CGTGGCGGCAGTCCAGTGCCCGTGGCGGGGAGGTGTGGGATCAACCCCCC	3	0.14947683109118087	No Hit
CGTGGCGGGGGGGGGGGGGGGGGGGCGGCGGGGGGGGGGGGGGGGGGGGG	3	0.14947683109118087	No Hit
CGTGGCGAGAAGCAGTGGTAGAGACGGGGCTTCACTATGTTGGCCAGGCT	3	0.14947683109118087	No Hit
CGTGGCGGCAGTCCAGTGCCCGTGGCGGGGAGCTGGGGGATCAACCCCCC	3	0.14947683109118087	No Hit
CGTGGCGGCAGTCCAGTGCCCGTGGCGGGGAGCTGTGGTATCAACCCCCC	3	0.14947683109118087	No Hit
CGTGGCGGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAAAAAA	3	0.14947683109118087	No Hit
CGTGGCGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGGATCGGAAGAG	3	0.14947683109118087	Illumina Single End Adapter 2 (97% over 34bp)
CGTGGCGAGGCTAAGCAATGCACTCTGATACCTGTTTCGGTCAGTTGTTC	3	0.14947683109118087	No Hit
CGTGGCGGGGGGCTCGTGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA	3	0.14947683109118087	No Hit
CGTGGCGGCAGTCCAGTGCCCGTGGCGGGAAGCTGTGGGATCAAACCCCC	3	0.14947683109118087	No Hit
CGTGGCGGGGCTCATCGTCTCTTTCCGCTGCCATGAAAGGAGTGCTTTTT	3	0.14947683109118087	No Hit
CGTGGCCTTGCTATTGACTCTACTGTAGACATTTTTACTTTTTATGTCGC	3	0.14947683109118087	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.04982561036372696	0.0	0.0	0.0	0.0
8	0.09965122072745392	0.0	0.0	0.0	0.0
9	0.09965122072745392	0.0	0.0	0.0	0.0
10	0.09965122072745392	0.0	0.0	0.0	0.0
11	0.09965122072745392	0.0	0.0	0.0	0.0
12	0.09965122072745392	0.0	0.0	0.0	0.0
13	0.09965122072745392	0.0	0.0	0.0	0.0
14	0.14947683109118087	0.0	0.0	0.0	0.0
15	0.14947683109118087	0.0	0.0	0.0	0.0
16	0.14947683109118087	0.0	0.0	0.0	0.0
17	0.14947683109118087	0.0	0.0	0.0	0.0
18	0.19930244145490783	0.0	0.0	0.0	0.0
19	0.19930244145490783	0.0	0.0	0.0	0.0
20	0.2491280518186348	0.0	0.0	0.0	0.0
21	0.2491280518186348	0.0	0.0	0.0	0.0
22	0.29895366218236175	0.0	0.0	0.0	0.0
23	0.29895366218236175	0.0	0.0	0.0	0.0
24	0.29895366218236175	0.0	0.0	0.0	0.0
25	0.29895366218236175	0.0	0.0	0.0	0.0
26	0.39860488290981566	0.0	0.0	0.0	0.0
27	0.4982561036372696	0.0	0.0	0.0	0.0
28	0.5480817140009965	0.0	0.0	0.0	0.0
29	0.5480817140009965	0.0	0.0	0.0	0.0
30	0.5480817140009965	0.0	0.0	0.0	0.0
31	0.5480817140009965	0.0	0.0	0.0	0.0
32	0.5979073243647235	0.0	0.0	0.0	0.0
33	0.5979073243647235	0.0	0.0	0.0	0.0
34	0.5979073243647235	0.0	0.0	0.0	0.0
35	0.5979073243647235	0.0	0.0	0.0	0.0
36	0.5979073243647235	0.0	0.0	0.0	0.0
37	0.5979073243647235	0.0	0.0	0.0	0.0
38	0.5979073243647235	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTAGACA	20	6.117847E-4	44.000004	25
ACTGTAG	20	6.117847E-4	44.000004	22
GCCTTGC	20	6.117847E-4	44.000004	5
TTACTTT	20	6.117847E-4	44.000004	35
TGACTCT	20	6.117847E-4	44.000004	15
TATGTCC	20	6.117847E-4	44.000004	43
TTTTTAT	20	6.117847E-4	44.000004	39
TTTTTAC	20	6.117847E-4	44.000004	32
CATTTTT	20	6.117847E-4	44.000004	30
CCTTGCT	20	6.117847E-4	44.000004	6
ACTCTAC	20	6.117847E-4	44.000004	17
TCTACTG	20	6.117847E-4	44.000004	19
ACATTTT	20	6.117847E-4	44.000004	29
TATTGAC	20	6.117847E-4	44.000004	12
TTTTATG	20	6.117847E-4	44.000004	40
GCTATTG	20	6.117847E-4	44.000004	10
CTCTACT	20	6.117847E-4	44.000004	18
GACATTT	20	6.117847E-4	44.000004	28
TTTTACT	20	6.117847E-4	44.000004	33
GGCCTTG	20	6.117847E-4	44.000004	4
>>END_MODULE