FastQCFastQC Report
Fri 17 Jun 2016
SRR1527104_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527104_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3734
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGCGGGGCTCATCGTCTCTTGCCGCTGCCATGAAAGGAGTGCTTTTTGC1754.686663095875736No Hit
CGTGGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1664.445634708087842No Hit
CGTGGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1283.4279592929833957No Hit
CGTGGCCTTATGGTTACAGTATGCCCATCGCAGTTCGCTACACGCAGGACGC1072.8655597214783075No Hit
CGTGGCGGGAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA962.570969469737547No Hit
CGTGGCGGGCTTTTACAATGGTTTTTTGCATTTTATTAAAAAATGCATGCAG882.3567220139260847No Hit
CGTGGCGGGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT842.2495982860203534No Hit
CGTGGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA842.2495982860203534No Hit
CGTGGCCTTGCTATTGACTCTACTGTAGACATTTTTACTTTTTATGTCCCTC701.8746652383502946No Hit
CGTGGCGGGGCTCATCGTCTCTTGCCGCTGCCATGAAAGGAGGGCTTTTTGC651.7407605784681306No Hit
CGTGGCGGCAGTCCAGTGCCCGTGGCGGGAAGCTGTGGTATCAACCCCTCGG541.44617032672737No Hit
CGTGGCGGGGGTTTGCTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA541.44617032672737No Hit
CGTGGCGGGGGAGGAGGAGGGAGAGGAAGAGGAGGAGGAAGAAAAGCAGAGC451.205141938939475No Hit
CGTGGCGGCAGTCCAGTGCCCGTGGCGGGAAGCTGTGGGATCAACCCCTCGG411.098018211033744No Hit
CGTGGCGGGGGGGTGCCGATGATCATCGGCTGTGCGCTGTTCATGGAATTGA401.0712372790573113No Hit
CGTGGCGGGGTGCTCGTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA391.0444563470808785No Hit
CGTGGCGGGGTTCAAAACGGATGAACGGGTTCTGACGGCTGGAGGCTTTCAC300.8034279592929834No Hit
CGTGGCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA290.7766470273165506No Hit
CGTGGCGGGGGGGGGCCGATGATCATCGGCTGTGCGCTGTTCATGGAATTGA270.723085163363685No Hit
CGTGGCGGGACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT270.723085163363685No Hit
CGTGGCGGGCTCTGTTTTGTGATTGAAGCCGATCAGATTACCTTTGAAACAG260.6963042313872523No Hit
CGTGGCGGGGTTCAAAACGGATGAACGGGGTCTGACGGCTGGAGGCTTTCAC230.6159614354579539No Hit
CGTGGCGGGGCAGTGGCATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA220.5891805034815212No Hit
CGTGGCGGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210.5623995715050883No Hit
CGTGGCGAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA210.5623995715050883No Hit
CGTGGCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200.5356186395286556No Hit
CGTGGCGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA190.5088377075522228No Hit
CGTGGCGGCGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190.5088377075522228No Hit
CGTGGCGGGGGGTTGCTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA170.45527584359935724No Hit
CGTGGCGAGCTCTGTTTTGTGATTGAAGCCGATCAGATTACCTTTGAAACAG140.3749330476700589No Hit
CGTGGCGGCAGTCCAGTGCCCGTGGCGGGGAGCTGTGGTATCAACCCCTCGG140.3749330476700589No Hit
CGTGGCCTTGCTATTAACTCTACTGTAGACATTTTTACTTTTTATGTCCCTC130.34815211569362614No Hit
CGTGGCGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA130.34815211569362614No Hit
CGTGGCGGGGCTCATAGTCTCTTGCCGCTGCCATGAAAGGAGTGCTTTTTGC120.32137118371719336No Hit
CGTGGCGGCAGTCCAGTGCCCGTGGCGGGGAGCTGTGGGATCAACCCCTCGG120.32137118371719336No Hit
CGTGGCGGGGTTCAAAACGGATGAACGGGGTCTGACGGCTGGGGGCTTTCAC120.32137118371719336No Hit
CGTGGCGGGGTTCAAAACGGATGAACGGGTTCTGACGGCTGGGGGCTTTCAC110.2945902517407606No Hit
CGTGGCGGGGGAATTGCCAGAACCCAGGAGGGGGAGGGTGCAGTGAGCCGAG100.2678093197643278No Hit
CGTGGCGGGATACCACTGCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100.2678093197643278No Hit
CGTGGCGGGGGGGGATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCG100.2678093197643278No Hit
CGTGGCGGGGCTCATGGTCTCTTGCCGCTGCCATGAAAGGAGTGCTTTTTGC90.241028387787895No Hit
CGTGGCGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAA90.241028387787895No Hit
CGTGGCGGGGGGGGATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.241028387787895No Hit
CGTGGCACGGGCCATGGCAATAAAGGGTTGTTCGAGGGTGTCTTCCAGGGAC80.21424745581146223No Hit
CGTGGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT80.21424745581146223No Hit
CGTGGCGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACC80.21424745581146223No Hit
CGTGGCGGGGGGATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.18746652383502946No Hit
CGTGGCGGGGAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.18746652383502946No Hit
CGTGGCGGCAGTCCAATGCCCGTGGCGGGAAGCTGTGGTATCAACCCCTCGG70.18746652383502946No Hit
CGTGGCGAGGCTAAGCAATGCACTCTGATACCTGTTTCGGTCAGTTGTTCCA70.18746652383502946No Hit
CGTGGCGGGCAGTGCTCGTGGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAA60.16068559185859668No Hit
CGTGGCGGGGGCGACTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.16068559185859668No Hit
CGTGGCGGGGGAGGAGGAGGGAGAGGAAGAGGAGGAGGAAGAAAGGCAGAGC60.16068559185859668No Hit
CGTGGCGGGGCTCATCGTCTCTTGCCGCTGCCATGAAAGGAGTGCTTTTTTC50.1339046598821639No Hit
CGTGGCGGGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAAAAAA50.1339046598821639No Hit
CGTGGCGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.1339046598821639No Hit
CGTGGCGGGGTCGACTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.1339046598821639No Hit
CGTGGCGGGGCTCATCGTCTCTTGCCGCCGCCATGAAAGGAGTGCTTTTTGC50.1339046598821639No Hit
CGTGGCGGGGGAGATCATTTTTTAGCTATTAGATTGCTTGCAGAATATTTTA50.1339046598821639No Hit
CGTGGCGGGGGAGATCATTTTTCAGCTATTAGATTGCTTGCAGAATATTTTA50.1339046598821639No Hit
CGTGGCGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA50.1339046598821639Illumina Single End Adapter 2 (97% over 34bp)
CGTGGCGGTAGTGCAGTGCTCGTGGCGGAAAAAAAAAAAAAAAAAAAAAAAA40.10712372790573112No Hit
CGTGGCGGAGATTCTTGATACATAACCGCGACATCGTGCAGCGGATGGATGA40.10712372790573112No Hit
CGTGGCGGGCTTTTACAATGGCTTTTTGCATTTTATTAAAAAATGCATGCAG40.10712372790573112No Hit
CGTGGCCTTATGGTTTCAGTATGCCCATCGCAGTTCGCTACACGCAGGACGC40.10712372790573112No Hit
CGTGGCGGGGCCGATGCCGCCGTGCCTGGACGCACCGTGCCTGAACGTGCGC40.10712372790573112No Hit
CGTGGCGGGGGGGATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.10712372790573112No Hit
CGTGGCGGCAGTCCAATGCCCGTGGCGGGAAGCTGTGGGATCAACCCCTCGG40.10712372790573112No Hit
CGTGGCGTTATACCACTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.10712372790573112No Hit
CGTGGCGAGCTCTGTATTGTGATTGAAGCCGATCAGATTACCTTTGAAACAG40.10712372790573112No Hit
CGTGGCGGGGTTCAAAACGGATGAACGGGGTCTGACGGCTGGAGGGTTTCAC40.10712372790573112No Hit
CGTGGCGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA40.10712372790573112Illumina Single End Adapter 2 (97% over 34bp)
CGTGGCGGGGTGCTCGTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.10712372790573112No Hit
CGTGGCGGGGGATGCTGAGGCGGGAGGATCACCTGAGGGCAGGGGTTCGAGA40.10712372790573112No Hit
CGTGGCAAAAGTGAGGGTAATGTCGCGTTCACGCAGTCGCGCCAAAGTAGAG40.10712372790573112No Hit
CGTGGCGGAAGTGCAGTGCTCGTGGGGGTGGTATCAACGCAAAAAAAAAAAA40.10712372790573112No Hit
CGTGGCGGTACCAACGCAGAGTCGTCAAAAAAAAAAAAAAAAAAAAAAAAAA40.10712372790573112No Hit
CGTGGCGGGGGAGGAAGAGGGAGAGGAAGAGGAGGAGGAAGAAAAGCAGAGC40.10712372790573112No Hit
CGTGGCGGGTACCTGGTTGATCCTGCCCGTAGCATATGCTTGTCTCAAAGAT40.10712372790573112No Hit
CGTGGCGGGGTTCAAAACGGATGAACGGGGTCTGACGGCTGGGGGCTTTTAC40.10712372790573112No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGGGT451.6916601E-1046.05
GCGGGGC403.4524419E-946.05
CGCTGCC301.4055531E-646.026
GCGGGCT252.7982775E-546.05
GGATGAA205.5271824E-446.019
CATGAAA301.4055531E-646.032
GGGTTCA205.5271824E-446.08
GAGTGCT205.5271824E-446.040
CGGGGTT252.7982775E-546.06
CGGGGTG205.5271824E-446.06
TTCAAAA205.5271824E-446.011
CGGGGCT301.4055531E-646.06
TGCCGCT252.7982775E-546.023
CCGCTGC301.4055531E-646.025
CTCTTGC252.7982775E-546.019
GACGGCT205.5271824E-446.034
GAACGGG205.5271824E-446.023
CCATGAA301.4055531E-646.031
ATGAACG205.5271824E-446.021
ATGAAAG301.4055531E-646.033
Produced by FastQC (version 0.11.5)