FastQCFastQC Report
Fri 17 Jun 2016
SRR1527103_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527103_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3818
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGCGGGGCTCATCGTCTCTTGCCGCTGCCATGAAAGGAGTGCTTTTTGC1864.871660555264536No Hit
CGTGGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1584.138292299633315No Hit
CGTGGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1283.352540597171294No Hit
CGTGGCGGGAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1002.6191723415400734No Hit
CGTGGCGGGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT902.357255107386066No Hit
CGTGGCGGGCTTTTACAATGGTTTTTTGCATTTTATTAAAAAATGCATGCAG882.3048716605552646No Hit
CGTGGCCTTATGGTTACAGTATGCCCATCGCAGTTCGCTACACGCAGGACGC842.2001047668936615No Hit
CGTGGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA822.1477213200628604No Hit
CGTGGCCTTGCTATTGACTCTACTGTAGACATTTTTACTTTTTATGTCCCTC731.9119958093242535No Hit
CGTGGCGGCAGTCCAGTGCCCGTGGCGGGAAGCTGTGGTATCAACCCCTCGG691.8072289156626504No Hit
CGTGGCGGGGGTTTGCTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA621.6238868517548455No Hit
CGTGGCGGGGCTCATCGTCTCTTGCCGCTGCCATGAAAGGAGGGCTTTTTGC501.3095861707700367No Hit
CGTGGCGGCAGTCCAGTGCCCGTGGCGGGAAGCTGTGGGATCAACCCCTCGG441.1524358302776323No Hit
CGTGGCGGGACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT380.9952854897852279No Hit
CGTGGCGGGGGAGGAGGAGGGAGAGGAAGAGGAGGAGGAAGAAAAGCAGAGC370.9690937663698271No Hit
CGTGGCGGGGGGTTGCTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA320.8381351492928235No Hit
CGTGGCGGGGCAGTGGCATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA290.7595599790466213No Hit
CGTGGCGGGGTGCTCGTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA280.7333682556312205No Hit
CGTGGCGGGCTCTGTTTTGTGATTGAAGCCGATCAGATTACCTTTGAAACAG260.6809848088004191No Hit
CGTGGCGGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240.6286013619696176No Hit
CGTGGCGGGGGGGTGCCGATGATCATCGGCTGTGCGCTGTTCATGGAATTGA240.6286013619696176No Hit
CGTGGCGAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA220.5762179151388162No Hit
CGTGGCGGGGTTCAAAACGGATGAACGGGTTCTGACGGCTGGAGGCTTTCAC220.5762179151388162No Hit
CGTGGCGGGGGGGGGCCGATGATCATCGGCTGTGCGCTGTTCATGGAATTGA220.5762179151388162No Hit
CGTGGCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA210.5500261917234154No Hit
CGTGGCGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA210.5500261917234154No Hit
CGTGGCGGGGTTCAAAACGGATGAACGGGGTCTGACGGCTGGAGGCTTTCAC210.5500261917234154No Hit
CGTGGCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210.5500261917234154No Hit
CGTGGCGGGGGCGACTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160.41906757464641176No Hit
CGTGGCGGGGCTCATCGTCTGTTGCCGCTGCCATGAAAGAAGTGCTTTTTGC130.34049240440020956No Hit
CGTGGCGGCAGTCCAGTGCCCGTGGCGGGGAGCTGTGGGATCAACCCCTCGG120.3143006809848088No Hit
CGTGGCGGGAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA120.3143006809848088No Hit
CGTGGCGGCGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110.2881089575694081No Hit
CGTGGCGGGGGGGGATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCG110.2881089575694081No Hit
CGTGGCGGGGGAATTGCCAGAACCCAGGAGGGGGAGGGTGCAGTGAGCCGAG100.26191723415400736No Hit
CGTGGCGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAA100.26191723415400736No Hit
CGTGGCGGCAGTCCAGTGCCCGTGGCGGGGAGCTGTGGTATCAACCCCTCGG90.23572551073860662No Hit
CGTGGCGGGGTGGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.20953378732320588No Hit
CGTGGCGGGGGGATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.20953378732320588No Hit
CGTGGCGAGCTCTGTTTTGTGATTGAAGCCGATCAGATTACCTTTGAAACAG80.20953378732320588No Hit
CGTGGCGGGATACCACTGCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.20953378732320588No Hit
CGTGGCGGGCTTTTTTTTTTGTTTTTTTTTTTTTTTTTTATTTTTTTTTTTT80.20953378732320588No Hit
CGTGGCGGGGGGGGATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.20953378732320588No Hit
CGTGGCGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.20953378732320588No Hit
CGTGGCGGGGGGGATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.18334206390780514No Hit
CGTGGCGGGGGAGATCATTTTTTAGCTATTAGATTGCTTGCAGAATATTTTA70.18334206390780514No Hit
CGTGGCACGGGCCATGGCAATAAAGGGTTGTTCGAGGGTGTCTTCCAGGGAC70.18334206390780514No Hit
CGTGGCGGGGTTCAAAACGGATGAACGGGTTCTGACGGCTGGGGGCTTTCAC70.18334206390780514No Hit
CGTGGCGGGGAAAAAAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.18334206390780514No Hit
CGTGGCGAGGCTAAGCAATGCACTCTGATACCTGTTTCGGTCAGTTGTTCCA70.18334206390780514No Hit
CGTGGCGGGGTCGACTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.1571503404924044No Hit
CGTGGCGGAAGCAGTGGTAGCAGTGCTCGGGGGGAAAAAAAAAAAAAAAAAA60.1571503404924044No Hit
CGTGGCGGGGCCGATGCCGCCGTGCCTGGACGCACCGTGCCTGAACGTGCGC60.1571503404924044No Hit
CGTGGCGGGAAAAAAAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.1571503404924044No Hit
CGTGGCGGGGTTCAAAACGGATGAACGGGGTCTGACGGCTGGGGGCTTTCAC60.1571503404924044No Hit
CGTGGCGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACC60.1571503404924044No Hit
CGTGGCGGGCTTTTTTTTTTGTTTTTTTTTTTTTTTTTTGTTTTTTTTTTTT50.13095861707700368No Hit
CGTGGCCTTGCTATTGACTCGACTGTAGACATTTTTACTGTTTATGTCCCTC50.13095861707700368No Hit
CGTGGCGGGATACCACTGCTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.13095861707700368No Hit
CGTGGCGGAAGTGCAGTGCTCGTGGGGGTGGTATCAACGCAAAAAAAAAAAA50.13095861707700368No Hit
CGTGGCGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCG50.13095861707700368No Hit
CGTGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.13095861707700368No Hit
CGTGGCGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG40.10476689366160294No Hit
CGTGGCGGCAGTCCAGTGCCGGTGGCGGGAAGCTGTGGTATCAACCCCTCGG40.10476689366160294No Hit
CGTGGCGGGGCATTGTTATAAATAAAATTCCACAAGTAACAAAAAAAAAAAA40.10476689366160294No Hit
CGTGGCGGGAGGAAAAAAAAGAAAAAAAAAAAAAAAAAATAAAAAAAAAAAA40.10476689366160294No Hit
CGTGGCGGGGAAAAAAAAAAGAAAAAAAAAAAAAAAAAAGAAAAAAAAAAAA40.10476689366160294No Hit
CGTGGCGGGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.10476689366160294No Hit
CGTGGCGGGACGCAGAGTCGGCTTTTTTTTTTTTTTTTTATTTTTTTTTTTT40.10476689366160294No Hit
CGTGGCGGCTGGTGAGATGGCTCAGTGGGTAAGGGCACTCGACTGTTCTTCC40.10476689366160294No Hit
CGTGGCGGGGTTCAAAACGGATGAACGGGGTCTGACGGCTGGAGGCTTTTAC40.10476689366160294No Hit
CGTGGCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.10476689366160294No Hit
CGTGGCGGGAGGAAAAAAAAGAAAAAAAAAAAAAAAAAAGAAAAAAAAAAAA40.10476689366160294No Hit
CGTGGCGGGGGCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.10476689366160294No Hit
CGTGGCGGGACGTTTCCATTCTGTGATCATGTCTTCTCCATGTTGTACCTCT40.10476689366160294No Hit
CGTGGCCTTGCTATTGACTCTACTGTAGACATTTTTACTATTTATGTCCCTC40.10476689366160294No Hit
CGTGGCGGGAAGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACTC40.10476689366160294No Hit
CGTGGCGGGGTTCAAAACGGGTGAACGGGGTCTGACGGCAGGAGGCTTTCAC40.10476689366160294No Hit
CGTGGCGGGGTTCAAAACGGATGAACGGGGTCTGACGGCTGGAGGGTTTCAC40.10476689366160294No Hit
CGTGGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT40.10476689366160294No Hit
CGTGGCGGGGCTCATCGTCTGTTGCCGCTGCCATGAAAGGAGTGCTTTTTGC40.10476689366160294No Hit
CGTGGCGGGGCAGTGGCATCGACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA40.10476689366160294No Hit
CGTGGCGGCAGTCCAGTGCCGGTGGCGGGAAGCTGTGGTGTCAACCCCTCGG40.10476689366160294No Hit
CGTGGCAGGCAGTGATATCAACGCAGAGTCAAAAAAAAAAAAAAAAAAAAAA40.10476689366160294No Hit
CGTGGCCTTATGGTTACAGTGTGCCCATCGCAGTTCGCTACACGCAGGACGC40.10476689366160294No Hit
CGTGGCGGGCTTTTACAATGGTTTTTTGCATTTTATTAAGAAATGCATGCAG40.10476689366160294No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGGAG205.54699E-446.0000045
GGCGAGG205.54699E-446.0000044
CGTGGCC205.54699E-446.0000041
AGTGCTT205.54699E-446.00000441
TGCTTTT205.54699E-446.00000443
CGGGAGG205.54699E-446.0000046
AAAGGAG205.54699E-446.00000436
GGGATAC205.54699E-446.0000047
GTGGCGA205.54699E-446.0000042
GTGGCCT205.54699E-446.0000042
TGGCGAG205.54699E-446.0000043
ACGGATG205.54699E-446.00000417
GAAAGGA205.54699E-446.00000435
TGGCCTT205.54699E-446.0000043
GTGCTTT205.54699E-446.00000442
CGGGGTT252.8134662E-546.06
CGGGGCT301.4163434E-646.06
CTCTTGC301.4163434E-646.019
CGTGGCA252.8134662E-546.01
CGGGATA252.8134662E-546.06
Produced by FastQC (version 0.11.5)