FastQCFastQC Report
Fri 17 Jun 2016
SRR1527102_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527102_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1909366
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC295631.5483149904209041No Hit
ATCTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG151650.794242696266719No Hit
ATCTCTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT59880.3136119528681248No Hit
ATCTCTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC49760.2606100663780543No Hit
ATCTCTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC43340.22698633996834552No Hit
ATCTCTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT39650.2076605532936064No Hit
ATCTCTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC33870.17738872484374396No Hit
ATCTCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT33420.17503192159072697No Hit
ATCTCTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC33300.17440344072325578No Hit
ATCTCTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA31220.1635097723537551No Hit
ATCTCTGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTC29790.15602037534972343No Hit
ATCTCTGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC29430.15413493274730983No Hit
ATCTCTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT27700.14507433357460015No Hit
ATCTCTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCT27200.14245566329347017No Hit
ATCTCTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT26460.13858003127739785No Hit
ATCTCTGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCC26290.13768968338181364No Hit
ATCTCTGGGGCTCTTCCGGTCTCTTTGGCCTCGCCGGTAGAAGCAAGATG24450.12805297674725538No Hit
ATCTCTGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCAT23130.12113968720507226No Hit
ATCTCTGGGGCTCTCAGCTCCTGAAGGCAAATCCCAATTCCAGAGCAAGG22700.1188876307633005No Hit
ATCTCTGGGTGATCCAGTTGTAAAGGAAAACCATGTGAGACCGAGGATCA22340.11700218816088691No Hit
ATCTCTGGGATGGTGCCTCCGTTTTCCCTTCCTAGTGACCCCAGGTTTAG20740.10862244326127102No Hit
ATCTCTGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAA19470.10197102074720091No Hit
ATCTCTGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTT19140.10024269836165513No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGATT207.85779E-444.00000438
TAACGTG207.85779E-444.00000426
TTATCGC207.85779E-444.00000444
ACGGGTA207.85779E-444.00000443
TAGTTCG207.85779E-444.00000410
CGTACGG207.85779E-444.00000423
TCGTACG207.85779E-444.00000436
CATAACG207.85779E-444.00000434
ATTGCGA207.85779E-444.00000410
TACGTAA207.85779E-444.00000425
ATTTCGG207.85779E-444.00000441
CCGATAT302.5286008E-644.031
CGTTACG254.4439497E-544.044
CGTTAAT454.802132E-1044.032
TACGAAC254.4439497E-544.043
AACGCGT254.4439497E-544.021
TCCCGTA302.5286008E-644.026
CGCGTTA254.4439497E-544.023
GCGCTAA254.4439497E-544.033
CGTCATA454.802132E-1044.012
Produced by FastQC (version 0.11.5)