##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527102_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1909366
Sequences flagged as poor quality	0
Sequence length	50
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.42419001909534	33.0	31.0	34.0	31.0	34.0
2	32.878778610282154	34.0	31.0	34.0	31.0	34.0
3	33.01727536784461	34.0	31.0	34.0	31.0	34.0
4	36.49815907479237	37.0	37.0	37.0	35.0	37.0
5	36.44594750299314	37.0	37.0	37.0	35.0	37.0
6	36.402818527196985	37.0	37.0	37.0	35.0	37.0
7	36.54207469914097	37.0	37.0	37.0	35.0	37.0
8	36.6727206832006	37.0	37.0	37.0	35.0	37.0
9	38.755161137257076	39.0	39.0	39.0	39.0	39.0
10	38.24765498076325	39.0	39.0	39.0	37.0	39.0
11	37.94505034655482	39.0	38.0	39.0	35.0	39.0
12	37.38820634702828	39.0	37.0	39.0	35.0	39.0
13	37.24155819261472	39.0	37.0	39.0	35.0	39.0
14	38.009212482049016	40.0	38.0	40.0	34.0	40.0
15	38.01001117648476	40.0	38.0	40.0	34.0	40.0
16	37.87495587540576	40.0	37.0	40.0	34.0	40.0
17	37.85391119355849	40.0	37.0	40.0	33.0	40.0
18	37.862646553882286	40.0	37.0	40.0	34.0	40.0
19	37.83055736825732	40.0	37.0	40.0	34.0	40.0
20	37.6228203497915	40.0	37.0	40.0	33.0	40.0
21	37.83838404999356	40.0	37.0	40.0	34.0	40.0
22	37.677547939996835	40.0	37.0	40.0	34.0	40.0
23	37.766611534928344	40.0	37.0	40.0	34.0	40.0
24	37.826016593989834	40.0	37.0	40.0	34.0	40.0
25	37.785847239345415	40.0	37.0	40.0	34.0	40.0
26	37.671647552119396	40.0	36.0	40.0	34.0	40.0
27	37.52358269708375	40.0	36.0	40.0	34.0	40.0
28	37.388655187114466	40.0	36.0	40.0	33.0	40.0
29	37.5725958250016	40.0	36.0	40.0	34.0	40.0
30	37.58413892360082	40.0	36.0	40.0	34.0	40.0
31	37.546546864247084	40.0	36.0	40.0	34.0	40.0
32	36.86864069015579	40.0	35.0	40.0	32.0	40.0
33	37.09835254215274	40.0	35.0	40.0	33.0	40.0
34	36.9887067225456	40.0	35.0	40.0	32.0	40.0
35	37.00278574144507	40.0	35.0	40.0	32.0	40.0
36	36.91384312908054	40.0	35.0	40.0	32.0	40.0
37	36.92575179405101	40.0	35.0	40.0	32.0	40.0
38	36.749018260511605	40.0	35.0	40.0	32.0	40.0
39	36.764953916640394	39.0	35.0	40.0	32.0	40.0
40	36.645608018577896	39.0	35.0	40.0	32.0	40.0
41	36.52385294385676	39.0	35.0	40.0	31.0	40.0
42	36.39709620889866	39.0	35.0	40.0	31.0	40.0
43	36.33435758256929	39.0	35.0	40.0	31.0	40.0
44	36.19348778599807	39.0	35.0	40.0	31.0	40.0
45	36.08311607098901	39.0	35.0	40.0	30.0	40.0
46	35.7679067292494	38.0	35.0	40.0	30.0	40.0
47	35.69882096989262	38.0	35.0	40.0	29.0	40.0
48	35.60857530719621	38.0	35.0	40.0	29.0	40.0
49	35.343189833693486	38.0	35.0	40.0	28.0	40.0
50	34.99785740397598	37.0	34.0	40.0	27.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	5.0
13	5.0
14	6.0
15	24.0
16	38.0
17	99.0
18	237.0
19	460.0
20	791.0
21	1348.0
22	2136.0
23	3148.0
24	4606.0
25	6280.0
26	8828.0
27	12099.0
28	16065.0
29	21458.0
30	29395.0
31	38808.0
32	48607.0
33	65366.0
34	129249.0
35	160837.0
36	140476.0
37	247841.0
38	582244.0
39	388908.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	0.0	100.0	0.0
3	0.0	0.0	0.0	100.0
4	0.0	0.0	100.0	0.0
5	0.0	0.0	0.0	100.0
6	0.0	0.0	100.0	0.0
7	99.26389178397437	0.46303327910940073	0.2144167226189217	0.05865821429731125
8	99.33134873041627	0.4313473687077281	0.1727274917433326	0.06457640913266498
9	97.83016980505572	1.0948136711348164	0.46135733012947755	0.6136591936799964
10	56.01126237714509	27.61146893785686	9.28313377320011	7.094134911797948
11	36.8988449569124	23.16187676956644	22.046218482993833	17.893059790527328
12	35.54075017571277	15.559038968956187	26.09541596529948	22.80479489003156
13	26.200634137195276	15.773508064980732	29.365087678318353	28.660770119505635
14	24.515519811288144	17.167635749248703	34.04119482592651	24.27564961353664
15	26.322821292512806	19.295200605855555	29.475281323748305	24.90669677788334
16	30.445132049067595	19.067638158425364	29.01868997353048	21.468539818976563
17	29.904324262608633	19.33542338137371	25.235863632221378	25.524388723796278
18	29.39080302047905	19.229681475421685	27.12272031658676	24.256795187512502
19	27.142988824562707	22.112051853861438	24.693170403160003	26.051788918415852
20	27.898160960234968	22.275509252809574	26.588930566481228	23.23739922047423
21	28.47489690295103	21.88558924794932	27.00440879328531	22.63510505581434
22	28.086286233231345	18.821745019027258	29.90291018065683	23.189058567084572
23	30.376103900457007	18.92251145144514	27.318020746153437	23.383363901944413
24	30.95137338781564	19.508255619928292	24.72092830813998	24.81944268411609
25	27.38254478188048	19.9585621614714	25.480447436478915	27.178445620169207
26	26.466691037758082	19.9847488642827	26.52100225938872	27.027557838570498
27	26.38959738468162	18.657187778561052	25.798877742664317	29.154337094093012
28	26.93061466476307	21.935658223724523	24.719566599593794	26.414160511918617
29	29.947532322247277	21.280047932140825	24.1837866600746	24.588633085537293
30	27.235794499325955	20.295166039407846	27.57297448472425	24.89606497654195
31	30.448536320433067	20.213411153230968	24.732083843537595	24.605968682798373
32	32.17989636350496	19.808407607551406	25.244086256904126	22.767609772039517
33	33.27271984522611	19.43833712342212	25.024484567128564	22.264458464223203
34	27.608169413302637	21.808338474655987	29.245676313498826	21.337815798542554
35	25.5909553223426	25.283732924960432	28.940286985313453	20.18502476738352
36	26.275842347669332	27.27664575571158	24.655461551111728	21.79205034550736
37	28.428860679408768	25.73545354845535	23.63737491921402	22.19831085292186
38	28.166469917239546	26.92134457196787	22.78782590660984	22.12435960418275
39	29.85745006457641	23.212678973020363	22.60404762628014	24.32582333612309
40	26.61516964269815	23.222053812626807	23.095991025293213	27.066785519381824
41	24.478806053946702	24.86045106071858	23.0264391426264	27.634303742708312
42	23.113955103421763	22.675432578143738	24.729255679633972	29.48135663880052
43	24.43041302715142	20.698703129729974	25.22303214784384	29.647851695274767
44	25.241205719594884	20.51571045048461	25.158246245088684	29.084837584831824
45	27.611992671913082	21.839972011652034	23.922181498989715	26.62585381744516
46	26.82696769503594	24.358556714637214	23.648530454611635	25.165945135715205
47	25.05035702950613	22.34757505894627	26.121550294705152	26.480517616842448
48	25.568068144085526	22.244556570086615	27.312469165157438	24.87490612067042
49	28.392146922067322	21.358974654414084	25.685908306736373	24.56297011678222
50	24.706473248188143	21.167497483457858	26.86059142144565	27.26543784690835
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	1.0
7	44.5
8	88.0
9	338.0
10	588.0
11	2019.5
12	3451.0
13	4733.5
14	6016.0
15	6200.0
16	6384.0
17	6533.5
18	6683.0
19	7467.0
20	8251.0
21	8211.0
22	8171.0
23	8805.0
24	9439.0
25	10002.5
26	10566.0
27	12227.0
28	13888.0
29	15437.0
30	16986.0
31	19126.5
32	21267.0
33	26112.0
34	30957.0
35	33135.0
36	35313.0
37	39505.5
38	43698.0
39	45923.5
40	48149.0
41	51545.0
42	54941.0
43	62376.0
44	69811.0
45	72738.0
46	75665.0
47	79209.0
48	82753.0
49	88394.0
50	94035.0
51	101413.0
52	108791.0
53	119956.0
54	131121.0
55	145624.0
56	160127.0
57	169518.0
58	178909.0
59	178453.0
60	177997.0
61	182646.0
62	187295.0
63	149314.0
64	111333.0
65	89171.0
66	67009.0
67	57112.5
68	47216.0
69	40162.0
70	33108.0
71	28516.5
72	23925.0
73	23514.5
74	23104.0
75	15653.5
76	8203.0
77	5502.0
78	2801.0
79	1799.5
80	798.0
81	563.5
82	329.0
83	234.0
84	139.0
85	97.5
86	56.0
87	29.0
88	2.0
89	1.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1909366.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	7.479280288532582
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.89812135375358	4.928705200924572
2	10.860847236106977	1.6246264129955699
3	4.865468817483681	1.0917061506322685
4	2.5929499236116027	0.7757359701128129
5	1.54713012149082	0.5785709910730654
6	0.9635595434615535	0.4324039140143671
7	0.6512093132499671	0.3409403886209523
8	0.47141587579662864	0.2820681174037639
9	0.371033067001797	0.2497554273978296
>10	4.797076604260549	9.64157122655585
>50	3.5216914931377117	19.131511687649517
>100	3.2692536030208386	44.43290935236132
>500	0.13390183736634143	6.6321581482178065
>1k	0.054146098169995986	7.081843294469133
>5k	7.317040293242701E-4	0.327700457071065
>10k+	0.0014634080586485403	2.4477932605000996
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATCTCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	29563	1.5483149904209041	No Hit
ATCTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	15165	0.794242696266719	No Hit
ATCTCTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	5988	0.3136119528681248	No Hit
ATCTCTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	4976	0.2606100663780543	No Hit
ATCTCTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	4334	0.22698633996834552	No Hit
ATCTCTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	3965	0.2076605532936064	No Hit
ATCTCTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	3387	0.17738872484374396	No Hit
ATCTCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT	3342	0.17503192159072697	No Hit
ATCTCTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC	3330	0.17440344072325578	No Hit
ATCTCTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	3122	0.1635097723537551	No Hit
ATCTCTGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTC	2979	0.15602037534972343	No Hit
ATCTCTGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC	2943	0.15413493274730983	No Hit
ATCTCTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	2770	0.14507433357460015	No Hit
ATCTCTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCT	2720	0.14245566329347017	No Hit
ATCTCTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT	2646	0.13858003127739785	No Hit
ATCTCTGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCC	2629	0.13768968338181364	No Hit
ATCTCTGGGGCTCTTCCGGTCTCTTTGGCCTCGCCGGTAGAAGCAAGATG	2445	0.12805297674725538	No Hit
ATCTCTGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCAT	2313	0.12113968720507226	No Hit
ATCTCTGGGGCTCTCAGCTCCTGAAGGCAAATCCCAATTCCAGAGCAAGG	2270	0.1188876307633005	No Hit
ATCTCTGGGTGATCCAGTTGTAAAGGAAAACCATGTGAGACCGAGGATCA	2234	0.11700218816088691	No Hit
ATCTCTGGGATGGTGCCTCCGTTTTCCCTTCCTAGTGACCCCAGGTTTAG	2074	0.10862244326127102	No Hit
ATCTCTGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAA	1947	0.10197102074720091	No Hit
ATCTCTGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTT	1914	0.10024269836165513	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	2.0949362249039733E-4	0.0	0.0	0.0	0.0
9	3.14240433735596E-4	0.0	0.0	0.0	0.0
10	0.004818353317279139	0.0	0.0	0.0	0.0
11	0.009270092795200082	0.0	0.0	0.0	0.0
12	0.01424556632934702	0.0	0.0	0.0	0.0
13	0.021315976088397928	0.0	0.0	0.0	0.0
14	0.03315236575910538	0.0	0.0	0.0	0.0
15	0.043731793694870444	0.0	0.0	0.0	0.0
16	0.059181948353537246	0.0	0.0	0.0	0.0
17	0.06599049108447516	0.0	0.0	0.0	0.0
18	0.07190868591982888	0.0	0.0	0.0	0.0
19	0.08955852361464486	0.0	0.0	0.0	0.0
20	0.09652418656245057	0.0	0.0	0.0	0.0
21	0.10485155805644386	0.0	0.0	0.0	0.0
22	0.1106126326749298	0.0	0.0	0.0	0.0
23	0.11663557432152871	0.0	0.0	0.0	0.0
24	0.1255390532773706	0.0	0.0	0.0	0.0
25	0.13056690021714015	0.0	0.0	0.0	0.0
26	0.13821341743803964	0.0	0.0	0.0	0.0
27	0.14528382719709054	0.0	0.0	0.0	0.0
28	0.15575850832161042	0.0	0.0	0.0	0.0
29	0.15994838077141837	0.0	0.0	0.0	0.0
30	0.16921847356661845	0.0	0.0	0.0	0.0
31	0.17696973759876314	0.0	0.0	0.0	0.0
32	0.18252131859475867	0.0	0.0	0.0	0.0
33	0.18959172835380958	0.0	0.0	0.0	0.0
34	0.1972382455747091	0.0	0.0	0.0	0.0
35	0.20991260973537812	0.0	0.0	0.0	0.0
36	0.22494377714906413	0.0	0.0	0.0	0.0
37	0.2344233635667546	0.0	0.0	0.0	0.0
38	0.2432220957113513	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGATT	20	7.85779E-4	44.000004	38
TAACGTG	20	7.85779E-4	44.000004	26
TTATCGC	20	7.85779E-4	44.000004	44
ACGGGTA	20	7.85779E-4	44.000004	43
TAGTTCG	20	7.85779E-4	44.000004	10
CGTACGG	20	7.85779E-4	44.000004	23
TCGTACG	20	7.85779E-4	44.000004	36
CATAACG	20	7.85779E-4	44.000004	34
ATTGCGA	20	7.85779E-4	44.000004	10
TACGTAA	20	7.85779E-4	44.000004	25
ATTTCGG	20	7.85779E-4	44.000004	41
CCGATAT	30	2.5286008E-6	44.0	31
CGTTACG	25	4.4439497E-5	44.0	44
CGTTAAT	45	4.802132E-10	44.0	32
TACGAAC	25	4.4439497E-5	44.0	43
AACGCGT	25	4.4439497E-5	44.0	21
TCCCGTA	30	2.5286008E-6	44.0	26
CGCGTTA	25	4.4439497E-5	44.0	23
GCGCTAA	25	4.4439497E-5	44.0	33
CGTCATA	45	4.802132E-10	44.0	12
>>END_MODULE