FastQCFastQC Report
Fri 17 Jun 2016
SRR1527101_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527101_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2044610
Sequences flagged as poor quality0
Sequence length52
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC257951.2616097935547612No Hit
ATCTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT132170.6464313487657792No Hit
ATCTCTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC53290.2606365028049359No Hit
ATCTCTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT45550.22278087263585722No Hit
ATCTCTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA43960.21500432845383716No Hit
ATCTCTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT36200.1770508801189469No Hit
ATCTCTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC34070.16663324546001437No Hit
ATCTCTGGGGGGCTGATGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC32210.1575361560395381No Hit
ATCTCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC32080.1569003379617629No Hit
ATCTCTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC31750.15528633822587193No Hit
ATCTCTGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA30790.15059106626691643No Hit
ATCTCTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTC28900.1413472495977228No Hit
ATCTCTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC27980.13684761397039044No Hit
ATCTCTGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG26910.1316143420994713No Hit
ATCTCTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG26290.12858197895931253No Hit
ATCTCTGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT23800.11640361731577172No Hit
ATCTCTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT23740.116110162818337No Hit
ATCTCTGGGGCTCTCAGCTCCTGAAGGCAAATCCCAATTCCAGAGCAAGGAA23160.11327343600980137No Hit
ATCTCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTT23030.11263761793202616No Hit
ATCTCTGGGGCTCTTCCGGTCTCTTTGGCCTCGCCGGTAGAAGCAAGATGAC22620.11063234553288892No Hit
ATCTCTGGGTGATCCAGTTGTAAAGGAAAACCATGTGAGACCGAGGATCAAA20680.10114398344916635No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGTA950.046.014
CCGTCTA206.312614E-446.034
CGTTAAT301.8621195E-646.032
CCGTATT206.312614E-446.034
CGCGTTA206.312614E-446.023
GACGATT206.312614E-446.018
TATCGTC301.8621195E-646.020
TATCGGC405.6152203E-946.015
ACGCGTT206.312614E-446.022
CATATCG351.02008926E-745.99999617
CGTTATA351.02008926E-745.99999621
GTATCGT351.02008926E-745.99999619
CTGGGTA55250.043.9601825
ATCTCTG2196050.042.5385551
TCTCTGG2186650.042.4773982
ACCGTAG650.042.4615421
TCTGGGT196500.042.4300274
TCTGGGA600350.042.310654
CTCTGGG2155500.042.2664343
CGCTTGA601.8189894E-1242.16666821
Produced by FastQC (version 0.11.5)