##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527101_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2044610
Sequences flagged as poor quality	0
Sequence length	52
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.3665740654697	33.0	31.0	34.0	31.0	34.0
2	32.84497190173187	34.0	31.0	34.0	31.0	34.0
3	32.90265820865593	34.0	31.0	34.0	31.0	34.0
4	36.45143670431036	37.0	37.0	37.0	35.0	37.0
5	36.42769183365043	37.0	37.0	37.0	35.0	37.0
6	36.42458219415928	37.0	37.0	37.0	35.0	37.0
7	36.530008656907675	37.0	37.0	37.0	35.0	37.0
8	36.62499743227315	37.0	37.0	37.0	35.0	37.0
9	38.71684428815275	39.0	39.0	39.0	39.0	39.0
10	38.1335741290515	39.0	39.0	39.0	37.0	39.0
11	37.852019700578595	39.0	38.0	39.0	35.0	39.0
12	37.319454565907435	39.0	37.0	39.0	35.0	39.0
13	37.21448882672001	39.0	37.0	39.0	35.0	39.0
14	37.994480609994085	40.0	38.0	40.0	34.0	40.0
15	37.902842595898484	40.0	37.0	40.0	34.0	40.0
16	32.83886217909528	40.0	34.0	40.0	3.0	40.0
17	34.473364602540336	40.0	34.0	40.0	17.0	40.0
18	36.5552027036941	40.0	34.0	40.0	31.0	40.0
19	37.43164026391341	40.0	36.0	40.0	33.0	40.0
20	37.70260783230054	40.0	37.0	40.0	33.0	40.0
21	37.92913024977869	40.0	37.0	40.0	34.0	40.0
22	37.96882877419166	40.0	37.0	40.0	34.0	40.0
23	37.955411545478114	40.0	37.0	40.0	34.0	40.0
24	37.94996551909655	40.0	37.0	40.0	34.0	40.0
25	37.902202865094075	40.0	37.0	40.0	34.0	40.0
26	37.81183453079071	40.0	37.0	40.0	34.0	40.0
27	37.68292192643096	40.0	36.0	40.0	34.0	40.0
28	37.59233007761871	40.0	36.0	40.0	34.0	40.0
29	37.380622710443554	40.0	35.0	40.0	33.0	40.0
30	37.34273969118805	40.0	35.0	40.0	33.0	40.0
31	37.290819276047756	40.0	35.0	40.0	33.0	40.0
32	37.26021881923692	40.0	35.0	40.0	33.0	40.0
33	36.93679234670671	39.0	35.0	40.0	33.0	40.0
34	36.70890292036134	39.0	35.0	40.0	31.0	40.0
35	36.6803928377539	39.0	35.0	40.0	31.0	40.0
36	36.7524911841378	39.0	35.0	40.0	32.0	40.0
37	36.51270168883063	39.0	35.0	40.0	31.0	40.0
38	36.53277691100014	39.0	35.0	40.0	31.0	40.0
39	36.209550965709845	38.0	35.0	40.0	31.0	40.0
40	36.17069563388617	38.0	35.0	40.0	30.0	40.0
41	36.15118090980676	38.0	35.0	40.0	31.0	40.0
42	36.05763055056955	38.0	35.0	40.0	30.0	40.0
43	36.00669418617731	38.0	35.0	40.0	30.0	40.0
44	36.2188833078191	38.0	35.0	40.0	31.0	40.0
45	36.25249216231946	39.0	35.0	40.0	31.0	40.0
46	36.15913939577719	38.0	35.0	40.0	31.0	40.0
47	35.867650065293624	38.0	35.0	40.0	30.0	40.0
48	35.84561114344545	38.0	35.0	40.0	30.0	40.0
49	35.788287252825725	38.0	35.0	40.0	30.0	40.0
50	35.30681107888546	37.0	35.0	40.0	29.0	40.0
51	35.38275172282245	37.0	35.0	40.0	29.0	40.0
52	35.070724001154254	36.0	34.0	40.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	2.0
13	2.0
14	7.0
15	13.0
16	39.0
17	81.0
18	188.0
19	371.0
20	741.0
21	1317.0
22	2136.0
23	3348.0
24	4968.0
25	7213.0
26	10015.0
27	13921.0
28	19190.0
29	25617.0
30	34208.0
31	44994.0
32	61220.0
33	94025.0
34	145288.0
35	178621.0
36	200926.0
37	357989.0
38	523108.0
39	315061.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	0.0	100.0	0.0
3	0.0	0.0	0.0	100.0
4	0.0	0.0	100.0	0.0
5	0.0	0.0	0.0	100.0
6	0.0	0.0	100.0	0.0
7	99.33116829126337	0.43842101916746956	0.17382288064716497	0.05658780892199491
8	99.34760174311972	0.4364646558512381	0.15875888311218275	0.05717471791686434
9	97.9054685245597	1.0526701913812415	0.4589139249049941	0.5829473591540685
10	56.310983512748145	27.358518250424286	9.275949936662737	7.054548300164823
11	37.0847741134006	23.09389076645424	21.902514415952187	17.918820704192974
12	35.614762717584284	15.48256146648994	26.143567721961645	22.75910809396413
13	26.241777160436463	15.682941979154949	29.369513012261507	28.70576784814708
14	24.67771359819232	17.062324844346843	33.98838898371817	24.27157257374267
15	26.373636047950466	19.27639011840889	29.405950279026317	24.94402355461433
16	28.83596382684228	27.04564684707597	25.42313693075941	18.695252395322335
17	30.094101075510732	19.236431397674863	25.219528418622623	25.44993910819178
18	29.53893407544715	19.23613794317743	26.978739221660852	24.246188759714567
19	27.232186089278642	21.97455749507241	24.575884887582472	26.21737152806648
20	27.942835063899718	22.16838419062804	26.613877463183687	23.27490328228855
21	28.657298946987446	21.895226962599224	26.83347924543067	22.613994844982663
22	27.991939783137127	18.777908745433113	29.93964619169426	23.2905052797355
23	30.425998112109397	18.89563290798734	27.306381168046716	23.37198781185654
24	31.165356718396175	19.394652280875082	24.624109243327577	24.81588175740117
25	27.585945485936193	19.840996571473287	25.253373503993426	27.319684438597093
26	26.527210568274633	19.915289468407178	26.44553239982197	27.111967563496215
27	26.42939240246306	18.502452790507725	25.86146013176107	29.206694675268146
28	27.038750666386253	21.843383334719093	24.57789015998161	26.539975838913044
29	30.175436880383057	21.252072522388133	24.062828607900773	24.509661989328038
30	27.408356605905283	20.12956016061743	27.53199876749111	24.930084465986177
31	30.5738991788165	20.074243987850984	24.647781239453977	24.70407559387854
32	32.32753434640347	19.238387760991095	25.472535104494256	22.96154278811118
33	33.69268466846978	19.339580653523168	24.956006279926246	22.011728398080805
34	27.768327456091875	21.547336655890366	29.338993744528295	21.345342143489468
35	25.70348379397538	25.11300443605382	28.857434914237924	20.32607685573288
36	26.20294334860927	27.341351162324358	24.677664689109413	21.778040799956962
37	28.73931947902045	25.6901316143421	23.407300169714517	22.163248736922935
38	28.206748475259342	26.90449523380987	22.776324091146964	22.11243219978382
39	30.195978695203486	23.170384572118888	22.546500310572675	24.087136422104948
40	26.721183991078984	23.16842820880266	23.061415135404793	27.048972664713567
41	24.55328889128	24.945441918018595	22.89385261736957	27.607416573331832
42	23.173563662507764	22.586899213052856	24.732687407378425	29.506849717060955
43	24.462660360655576	20.620607352991524	25.176781880162963	29.739950406189937
44	25.27469786414035	20.42893265708375	25.18763969656805	29.108729782207853
45	27.78490763519693	21.674549180528317	23.827527010041035	26.713016174233715
46	26.572451469962488	24.34992492455774	23.77245538268912	25.305168222790652
47	25.250830231682325	22.32406180151716	26.066242461887594	26.358865504912917
48	25.67164398100371	22.156890556145182	27.28652408038697	24.884941382464138
49	28.433099710947317	21.2120648925712	25.796215415164752	24.55861998131673
50	24.692533050312772	21.153031629503914	26.811567976288874	27.342867343894433
51	23.468289796098034	22.07090838839681	27.730080553259544	26.73072126224561
52	22.991083874186273	23.311682912633707	26.454531671076637	27.242701542103383
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	42.0
8	84.0
9	355.5
10	627.0
11	2103.5
12	3580.0
13	4808.0
14	6297.0
15	6558.0
16	6815.5
17	7073.0
18	7827.0
19	8581.0
20	8160.0
21	7739.0
22	8407.5
23	9076.0
24	9865.0
25	10654.0
26	11470.0
27	12286.0
28	13968.0
29	15650.0
30	17574.0
31	19498.0
32	21988.5
33	24479.0
34	29005.5
35	33532.0
36	36858.0
37	40184.0
38	41361.5
39	44744.0
40	46949.0
41	54256.5
42	61564.0
43	64782.0
44	68000.0
45	74440.0
46	80880.0
47	83651.5
48	86423.0
49	90716.0
50	95009.0
51	105665.0
52	116321.0
53	126018.5
54	135716.0
55	150422.5
56	165129.0
57	177441.5
58	189754.0
59	189847.0
60	189940.0
61	191601.0
62	193262.0
63	160189.0
64	101301.0
65	75486.0
66	62785.5
67	50085.0
68	45463.0
69	40841.0
70	35880.0
71	30919.0
72	28211.0
73	25503.0
74	18219.0
75	10935.0
76	7558.0
77	4181.0
78	2896.0
79	1611.0
80	1079.0
81	547.0
82	384.5
83	222.0
84	120.0
85	18.0
86	13.0
87	8.0
88	7.5
89	5.5
90	4.0
91	4.0
92	4.0
93	2.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2044610.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.937098803694347
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.03931017649373	6.259482351899194
2	10.167710519593772	1.8173966704194386
3	4.2125883565550835	1.129449550854755
4	2.1599680761841054	0.7721539243873179
5	1.276576205747363	0.5704443840604714
6	0.7455411822446005	0.3997785124765853
7	0.5214737160051527	0.3262323486427387
8	0.3917009892097749	0.28005363536580574
9	0.3010172273570118	0.24211986322533657
>10	4.315663487129102	10.404491581491056
>50	3.056523707122855	19.793949023246505
>100	2.661646750103074	42.42681999287382
>500	0.10626465881583264	6.340946467382917
>1k	0.04212859254828868	6.744933281700928
>5k	6.287849634072937E-4	0.29946386611499287
>10k+	0.0012575699268145874	2.1922845458581537
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATCTCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	25795	1.2616097935547612	No Hit
ATCTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	13217	0.6464313487657792	No Hit
ATCTCTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	5329	0.2606365028049359	No Hit
ATCTCTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	4555	0.22278087263585722	No Hit
ATCTCTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	4396	0.21500432845383716	No Hit
ATCTCTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	3620	0.1770508801189469	No Hit
ATCTCTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC	3407	0.16663324546001437	No Hit
ATCTCTGGGGGGCTGATGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	3221	0.1575361560395381	No Hit
ATCTCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	3208	0.1569003379617629	No Hit
ATCTCTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	3175	0.15528633822587193	No Hit
ATCTCTGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA	3079	0.15059106626691643	No Hit
ATCTCTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTC	2890	0.1413472495977228	No Hit
ATCTCTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	2798	0.13684761397039044	No Hit
ATCTCTGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	2691	0.1316143420994713	No Hit
ATCTCTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	2629	0.12858197895931253	No Hit
ATCTCTGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT	2380	0.11640361731577172	No Hit
ATCTCTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT	2374	0.116110162818337	No Hit
ATCTCTGGGGCTCTCAGCTCCTGAAGGCAAATCCCAATTCCAGAGCAAGGAA	2316	0.11327343600980137	No Hit
ATCTCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTT	2303	0.11263761793202616	No Hit
ATCTCTGGGGCTCTTCCGGTCTCTTTGGCCTCGCCGGTAGAAGCAAGATGAC	2262	0.11063234553288892	No Hit
ATCTCTGGGTGATCCAGTTGTAAAGGAAAACCATGTGAGACCGAGGATCAAA	2068	0.10114398344916635	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	4.890908290578643E-5	0.0	0.0	0.0	0.0
8	1.467272487173593E-4	0.0	0.0	0.0	0.0
9	2.934544974347186E-4	0.0	0.0	0.0	0.0
10	0.004010544798274488	0.0	0.0	0.0	0.0
11	0.007629816933302684	0.0	0.0	0.0	0.0
12	0.011835998063200317	0.0	0.0	0.0	0.0
13	0.016677997270873175	0.0	0.0	0.0	0.0
14	0.029736722406718152	0.0	0.0	0.0	0.0
15	0.04015435706565066	0.0	0.0	0.0	0.0
16	0.057517081497204844	0.0	0.0	0.0	0.0
17	0.06534253476213067	0.0	0.0	0.0	0.0
18	0.07336362435867964	0.0	0.0	0.0	0.0
19	0.08876998547400237	0.0	0.0	0.0	0.0
20	0.09581289341243562	0.0	0.0	0.0	0.0
21	0.10422525567223088	0.0	0.0	0.0	0.0
22	0.11092580003032364	0.0	0.0	0.0	0.0
23	0.11689270814482958	0.0	0.0	0.0	0.0
24	0.12535397948753063	0.0	0.0	0.0	0.0
25	0.1301959786952035	0.0	0.0	0.0	0.0
26	0.13674979580457886	0.0	0.0	0.0	0.0
27	0.14379270374301212	0.0	0.0	0.0	0.0
28	0.15509070189424878	0.0	0.0	0.0	0.0
29	0.15963924660448692	0.0	0.0	0.0	0.0
30	0.16707342720616647	0.0	0.0	0.0	0.0
31	0.17387178973007078	0.0	0.0	0.0	0.0
32	0.18052342500525773	0.0	0.0	0.0	0.0
33	0.1877619692753141	0.0	0.0	0.0	0.0
34	0.19636996786673253	0.0	0.0	0.0	0.0
35	0.20791251143249814	0.0	0.0	0.0	0.0
36	0.22116687289996625	0.0	0.0	0.0	0.0
37	0.2300194169059136	0.0	0.0	0.0	0.0
38	0.23892086999476672	0.0	0.0	0.0	0.0
39	0.2458659597673884	0.0	0.0	0.0	0.0
40	0.2515394133844596	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCGTA	95	0.0	46.0	14
CCGTCTA	20	6.312614E-4	46.0	34
CGTTAAT	30	1.8621195E-6	46.0	32
CCGTATT	20	6.312614E-4	46.0	34
CGCGTTA	20	6.312614E-4	46.0	23
GACGATT	20	6.312614E-4	46.0	18
TATCGTC	30	1.8621195E-6	46.0	20
TATCGGC	40	5.6152203E-9	46.0	15
ACGCGTT	20	6.312614E-4	46.0	22
CATATCG	35	1.02008926E-7	45.999996	17
CGTTATA	35	1.02008926E-7	45.999996	21
GTATCGT	35	1.02008926E-7	45.999996	19
CTGGGTA	5525	0.0	43.960182	5
ATCTCTG	219605	0.0	42.538555	1
TCTCTGG	218665	0.0	42.477398	2
ACCGTAG	65	0.0	42.46154	21
TCTGGGT	19650	0.0	42.430027	4
TCTGGGA	60035	0.0	42.31065	4
CTCTGGG	215550	0.0	42.266434	3
CGCTTGA	60	1.8189894E-12	42.166668	21
>>END_MODULE