FastQCFastQC Report
Fri 17 Jun 2016
SRR1527100_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527100_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1982645
Sequences flagged as poor quality0
Sequence length52
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC247521.2484332797853372No Hit
ATCTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT129500.6531678641410843No Hit
ATCTCTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC51850.26151933402096694No Hit
ATCTCTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT48320.2437148354849204No Hit
ATCTCTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA42730.2155201763301045No Hit
ATCTCTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT36000.18157562246393075No Hit
ATCTCTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC31780.16029092449732554No Hit
ATCTCTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC30750.15509584418794087No Hit
ATCTCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC29790.1502538275889027No Hit
ATCTCTGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA26940.1358790908105082No Hit
ATCTCTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC26600.13416420993168218No Hit
ATCTCTGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG25990.13108751188437667No Hit
ATCTCTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTC25040.12629593295824518No Hit
ATCTCTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG24380.12296704654640644No Hit
ATCTCTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT23960.12084866428432725No Hit
ATCTCTGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT22570.11383782775030325No Hit
ATCTCTGGGGCTCTTCCGGTCTCTTTGGCCTCGCCGGTAGAAGCAAGATGAC22490.11343432636705007No Hit
ATCTCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTT21800.1099541269364914No Hit
ATCTCTGGGGCTCTCAGCTCCTGAAGGCAAATCCCAATTCCAGAGCAAGGAA20550.10364941782316048No Hit
ATCTCTGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA20020.10097622115910816No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGCTA301.8620922E-646.013
CGTTAAT600.046.032
AATTACG206.3125667E-446.023
CATAACG301.8620922E-646.034
ACGTATC206.3125667E-446.040
ACCGTAG253.418058E-546.021
TACGTAA301.8620922E-646.025
ACGAATC301.8620922E-646.021
CTGGGTA53850.043.4373255
ACGGTAC700.042.71428735
ATCTCTG2123000.042.6512951
TCTCTGG2117050.042.5527952
TCTGGGT193800.042.4514964
CTCTGGG2083450.042.4243473
TCTGGGA577800.042.389584
TGGGATT65550.042.140356
TCTGGGG1179950.042.0566984
CTGGGAT192000.041.8671885
AACCCGA4450.041.8651740
CTGGGGG482600.041.834655
Produced by FastQC (version 0.11.5)