##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527100_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1982645
Sequences flagged as poor quality	0
Sequence length	52
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.28517208073054	33.0	31.0	34.0	31.0	34.0
2	32.80195950359242	34.0	31.0	34.0	31.0	34.0
3	32.88474487364102	34.0	31.0	34.0	31.0	34.0
4	36.44812560998061	37.0	37.0	37.0	35.0	37.0
5	36.44332545665008	37.0	37.0	37.0	35.0	37.0
6	36.42020734927332	37.0	37.0	37.0	35.0	37.0
7	36.53458586887718	37.0	37.0	37.0	35.0	37.0
8	36.6481775607837	37.0	37.0	37.0	35.0	37.0
9	38.664897649352255	39.0	39.0	39.0	39.0	39.0
10	38.13353878278764	39.0	39.0	39.0	37.0	39.0
11	37.78607516726393	39.0	38.0	39.0	35.0	39.0
12	37.29180766097814	39.0	37.0	39.0	35.0	39.0
13	37.12542033495659	39.0	37.0	39.0	34.0	39.0
14	37.92963843754177	40.0	37.0	40.0	34.0	40.0
15	38.020312764009695	40.0	38.0	40.0	34.0	40.0
16	38.07642114448124	40.0	38.0	40.0	35.0	40.0
17	37.97669678636367	40.0	38.0	40.0	34.0	40.0
18	38.00784608439736	40.0	38.0	40.0	34.0	40.0
19	37.974057382940465	40.0	37.0	40.0	34.0	40.0
20	37.953841459262755	40.0	37.0	40.0	34.0	40.0
21	33.74227257022815	40.0	34.0	40.0	10.0	40.0
22	34.531510179583336	40.0	34.0	40.0	18.0	40.0
23	36.58943986442353	39.0	34.0	40.0	30.0	40.0
24	37.37770705295199	39.0	36.0	40.0	33.0	40.0
25	37.63830438631222	39.0	36.0	40.0	34.0	40.0
26	37.71146826587715	40.0	36.0	40.0	34.0	40.0
27	37.658272661015964	40.0	36.0	40.0	34.0	40.0
28	37.57511960033188	40.0	36.0	40.0	34.0	40.0
29	37.52992895853771	40.0	36.0	40.0	34.0	40.0
30	37.420387411765596	40.0	36.0	40.0	33.0	40.0
31	37.28012982657006	40.0	35.0	40.0	33.0	40.0
32	37.27957904718192	40.0	35.0	40.0	33.0	40.0
33	36.9743842190609	39.0	35.0	40.0	33.0	40.0
34	36.73390445591621	39.0	35.0	40.0	31.0	40.0
35	36.70119461628279	39.0	35.0	40.0	31.0	40.0
36	36.711280637733935	39.0	35.0	40.0	31.0	40.0
37	36.67425232454625	39.0	35.0	40.0	32.0	40.0
38	36.624451175071684	39.0	35.0	40.0	32.0	40.0
39	36.51851340002875	39.0	35.0	40.0	31.0	40.0
40	31.610550048041883	37.0	31.0	40.0	9.0	40.0
41	32.55957773580243	37.0	31.0	40.0	15.0	40.0
42	34.3626973058717	37.0	32.0	40.0	24.0	40.0
43	35.27830045217374	37.0	34.0	40.0	29.0	40.0
44	35.9365453724696	38.0	35.0	40.0	30.0	40.0
45	36.14868017219422	38.0	35.0	40.0	31.0	40.0
46	36.14597267791259	38.0	35.0	40.0	31.0	40.0
47	36.10294429915592	38.0	35.0	40.0	31.0	40.0
48	35.974688358228526	38.0	35.0	40.0	31.0	40.0
49	35.855955049945905	38.0	35.0	40.0	31.0	40.0
50	35.765555608795324	37.0	35.0	40.0	30.0	40.0
51	35.581941799969236	37.0	35.0	40.0	30.0	40.0
52	35.20973043585715	37.0	34.0	40.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	1.0
14	6.0
15	11.0
16	35.0
17	111.0
18	216.0
19	420.0
20	737.0
21	1412.0
22	2298.0
23	3671.0
24	5225.0
25	7558.0
26	10275.0
27	14489.0
28	19628.0
29	27182.0
30	36944.0
31	49914.0
32	73922.0
33	80516.0
34	156027.0
35	204648.0
36	242880.0
37	226559.0
38	497407.0
39	320551.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	0.0	100.0	0.0
3	0.0	0.0	0.0	100.0
4	0.0	0.0	100.0	0.0
5	0.0	0.0	0.0	100.0
6	0.0	0.0	100.0	0.0
7	99.32514393650905	0.4557548123844662	0.1663938829190299	0.05270736818744657
8	99.37048740445213	0.43406661303460786	0.13935929024106686	0.05608669227219194
9	97.8807602974814	1.0408318181015765	0.48995155461517315	0.5884563298018556
10	56.30957634876642	27.38084730246716	9.22227630261595	7.08730004615047
11	37.09902680510127	23.09868887269279	21.885208900231763	17.91707542197418
12	35.673204229703245	15.494402679249184	26.15188296442379	22.68051012662378
13	26.370278088109572	15.69574986949252	29.295007426947333	28.638964615450575
14	24.67602621750238	17.012173132356022	33.965283749738354	24.346516900403252
15	26.341528614552782	19.237533698670212	29.416108279596198	25.00482940718081
16	30.377399887523985	18.967086896544767	28.993037079255235	21.662476136676005
17	30.109172343006442	19.256750452047644	25.142423378870145	25.491653826075773
18	29.55758595209934	19.195569554811883	26.93523046233693	24.311614030751848
19	27.19009202353422	22.052762849627644	24.57409168055804	26.1830534462801
20	27.95558458523841	22.170837441902105	26.602644447190492	23.270933525668994
21	33.282105470217815	20.988174887587036	25.302109051292593	20.427610590902557
22	28.104678346350454	18.78561214942665	29.799636344378342	23.310073159844553
23	30.449223133743054	18.943885567007708	27.21344466609	23.39344663315924
24	31.25390576729571	19.368369022190056	24.58902123173841	24.78870397877583
25	27.60020074193817	19.84207964612929	25.233665129158272	27.324054482774272
26	26.5730879708672	19.875772011630925	26.433426054588693	27.117713962913182
27	26.513067140108287	18.545125325007756	25.78731946465454	29.154488070229416
28	27.02622002426052	21.85434104441289	24.556741121078154	26.56269781024843
29	29.980152775711232	21.268003096873116	24.047017998683575	24.704826128732073
30	27.392498404908594	20.17764148397721	27.43466429945855	24.99519581165564
31	30.661363985988416	20.0652663487412	24.599360954684276	24.67400871058611
32	32.24208065488274	19.320402795255834	25.457759709882506	22.97975683997892
33	33.62528339667465	19.423093897293768	24.874548898062944	22.07707380796865
34	27.840687566357065	21.53058162202512	29.245225443788474	21.38350536782934
35	25.64609398051593	25.202746835666495	28.853980415051613	20.297178768765967
36	26.408762032537343	27.269733109053814	24.588466417336438	21.733038441072406
37	28.699641135957272	25.64226071737502	23.433695896138744	22.224402250528964
38	28.142557038703348	26.950462639554733	22.765598480817292	22.141381840924623
39	29.98201896960878	23.138534634289044	22.571917816855766	24.30752857924641
40	27.384932753972596	27.51173306365991	21.194616282793945	23.90871789957355
41	24.573486428483164	24.929626836877	22.89320579327111	27.603680941368726
42	23.172327875136496	22.553558503917746	24.725253386259265	29.54886023468649
43	24.44940975313281	20.641012384970583	25.10474643720888	29.804831424687727
44	25.26150672460274	20.485260851034855	25.131125340139054	29.122107084223348
45	27.735171954636357	21.74499216955128	23.80083171722623	26.71900415858613
46	26.615203427744248	24.280493986568448	23.76325565091078	25.34104693477652
47	25.1018714898532	22.337080011802417	26.053327751564197	26.507720746780183
48	25.6663699250244	22.114448123592474	27.271599302951362	24.947582648431766
49	28.395401092984372	21.26674215505045	25.75604810745242	24.58180864451276
50	24.675370527754588	21.09550625553238	26.887465986094334	27.341657230618694
51	23.555502876208298	22.0653218301814	27.66879597709121	26.710379316519095
52	23.023990679118047	23.30346582469378	26.43509049779461	27.237452998393557
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	1.0
5	1.0
6	1.0
7	40.0
8	79.0
9	289.0
10	499.0
11	1824.5
12	3150.0
13	4355.0
14	5900.0
15	6240.0
16	6380.0
17	6520.0
18	7291.5
19	8063.0
20	7761.0
21	7459.0
22	8062.0
23	8665.0
24	9484.5
25	10304.0
26	11077.0
27	11850.0
28	13476.0
29	15102.0
30	16857.5
31	18613.0
32	20977.0
33	23341.0
34	27709.5
35	32078.0
36	35465.5
37	38853.0
38	39947.0
39	42880.5
40	44720.0
41	51622.5
42	58525.0
43	62023.0
44	65521.0
45	71851.0
46	78181.0
47	80656.0
48	83131.0
49	87914.0
50	92697.0
51	102836.5
52	112976.0
53	122698.5
54	132421.0
55	145746.5
56	159072.0
57	170920.0
58	182768.0
59	182258.5
60	181749.0
61	186336.0
62	190923.0
63	158582.5
64	100341.0
65	74440.0
66	61889.0
67	49338.0
68	44567.5
69	39797.0
70	34635.5
71	29474.0
72	27526.5
73	25579.0
74	18214.5
75	10850.0
76	7626.0
77	4402.0
78	3023.5
79	1645.0
80	1097.5
81	550.0
82	365.0
83	180.0
84	101.5
85	23.0
86	14.5
87	6.0
88	6.5
89	4.5
90	2.0
91	4.5
92	7.0
93	3.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1982645.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.037206331845663
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.40058565662945	6.362246184617345
2	9.68555135821008	1.7506065212366502
3	4.097888457606102	1.1110039054882537
4	2.1384570263940677	0.7730270951723326
5	1.248524158656938	0.564158521603838
6	0.752748629441863	0.408164680816809
7	0.5673605002975172	0.3589147734009492
8	0.37517258749234084	0.2712409666576563
9	0.3149055197294371	0.2561279541148817
>10	4.562065971208156	11.287938840939047
>50	3.119477902848953	20.327448974213457
>100	2.5962064480055678	41.69490614602505
>500	0.09880405110086288	5.975288851959409
>1k	0.04030165242272038	6.339561944667612
>5k	6.500266519793611E-4	0.30458893496081807
>10k+	0.0013000533039587221	2.214775704125895
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATCTCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	24752	1.2484332797853372	No Hit
ATCTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	12950	0.6531678641410843	No Hit
ATCTCTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	5185	0.26151933402096694	No Hit
ATCTCTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	4832	0.2437148354849204	No Hit
ATCTCTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	4273	0.2155201763301045	No Hit
ATCTCTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	3600	0.18157562246393075	No Hit
ATCTCTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	3178	0.16029092449732554	No Hit
ATCTCTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC	3075	0.15509584418794087	No Hit
ATCTCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	2979	0.1502538275889027	No Hit
ATCTCTGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA	2694	0.1358790908105082	No Hit
ATCTCTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	2660	0.13416420993168218	No Hit
ATCTCTGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	2599	0.13108751188437667	No Hit
ATCTCTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTC	2504	0.12629593295824518	No Hit
ATCTCTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	2438	0.12296704654640644	No Hit
ATCTCTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT	2396	0.12084866428432725	No Hit
ATCTCTGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT	2257	0.11383782775030325	No Hit
ATCTCTGGGGCTCTTCCGGTCTCTTTGGCCTCGCCGGTAGAAGCAAGATGAC	2249	0.11343432636705007	No Hit
ATCTCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTT	2180	0.1099541269364914	No Hit
ATCTCTGGGGCTCTCAGCTCCTGAAGGCAAATCCCAATTCCAGAGCAAGGAA	2055	0.10364941782316048	No Hit
ATCTCTGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA	2002	0.10097622115910816	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	1.0087534581329487E-4	0.0	0.0	0.0	0.0
8	2.0175069162658974E-4	0.0	0.0	0.0	0.0
9	2.521883645332372E-4	0.0	0.0	0.0	0.0
10	0.003984576159625147	0.0	0.0	0.0	0.0
11	0.008725717412850005	0.0	0.0	0.0	0.0
12	0.012155479170502031	0.0	0.0	0.0	0.0
13	0.017754060863139895	0.0	0.0	0.0	0.0
14	0.029304287958762158	0.0	0.0	0.0	0.0
15	0.04024926297950465	0.0	0.0	0.0	0.0
16	0.05573362856184541	0.0	0.0	0.0	0.0
17	0.06309752880621594	0.0	0.0	0.0	0.0
18	0.06985617697570669	0.0	0.0	0.0	5.0437672906647434E-5
19	0.08221340683783532	0.0	0.0	0.0	5.0437672906647434E-5
20	0.08887117966151278	0.0	0.0	0.0	5.0437672906647434E-5
21	0.09618464223297665	0.0	0.0	0.0	5.0437672906647434E-5
22	0.10294329040246741	0.0	0.0	0.0	5.0437672906647434E-5
23	0.10934887486161164	0.0	0.0	0.0	5.0437672906647434E-5
24	0.12029384988235413	0.0	0.0	0.0	5.0437672906647434E-5
25	0.12458105207941916	0.0	0.0	0.0	5.0437672906647434E-5
26	0.1316423262863498	0.0	0.0	0.0	5.0437672906647434E-5
27	0.13895578885781368	0.0	0.0	0.0	5.0437672906647434E-5
28	0.15217045915935531	0.0	0.0	0.0	5.0437672906647434E-5
29	0.15686116273967352	0.0	0.0	0.0	5.0437672906647434E-5
30	0.16538512946089692	0.0	0.0	0.0	5.0437672906647434E-5
31	0.17264815435945416	0.0	0.0	0.0	5.0437672906647434E-5
32	0.17935636485603826	0.0	0.0	0.0	5.0437672906647434E-5
33	0.1858628246609958	0.0	0.0	0.0	5.0437672906647434E-5
34	0.19388241465315273	0.0	0.0	0.0	5.0437672906647434E-5
35	0.20583614313202817	0.0	0.0	0.0	5.0437672906647434E-5
36	0.2172350572089305	0.0	0.0	0.0	5.0437672906647434E-5
37	0.2250024588365542	0.0	0.0	0.0	5.0437672906647434E-5
38	0.23256810977255132	0.0	0.0	0.0	5.0437672906647434E-5
39	0.23801537844646925	0.0	0.0	0.0	5.0437672906647434E-5
40	0.24482446428886664	0.0	0.0	0.0	5.0437672906647434E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGCTA	30	1.8620922E-6	46.0	13
CGTTAAT	60	0.0	46.0	32
AATTACG	20	6.3125667E-4	46.0	23
CATAACG	30	1.8620922E-6	46.0	34
ACGTATC	20	6.3125667E-4	46.0	40
ACCGTAG	25	3.418058E-5	46.0	21
TACGTAA	30	1.8620922E-6	46.0	25
ACGAATC	30	1.8620922E-6	46.0	21
CTGGGTA	5385	0.0	43.437325	5
ACGGTAC	70	0.0	42.714287	35
ATCTCTG	212300	0.0	42.651295	1
TCTCTGG	211705	0.0	42.552795	2
TCTGGGT	19380	0.0	42.451496	4
CTCTGGG	208345	0.0	42.424347	3
TCTGGGA	57780	0.0	42.38958	4
TGGGATT	6555	0.0	42.14035	6
TCTGGGG	117995	0.0	42.056698	4
CTGGGAT	19200	0.0	41.867188	5
AACCCGA	445	0.0	41.86517	40
CTGGGGG	48260	0.0	41.83465	5
>>END_MODULE