FastQCFastQC Report
Fri 17 Jun 2016
SRR1527099_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527099_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences182579
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20841.1414237124751476No Hit
ATGGCGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19811.0850097765898599No Hit
ATGGCGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT17370.9513689964344202No Hit
ATGGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9830.5383970774294963No Hit
ATGGCGGAGAGAGAAAATGCCTAGAGATATTTCAAGCTCATGTCATTATG8010.4387142004283078No Hit
ATGGCGGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7860.43049857869744057No Hit
ATGGCGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT7680.42063983262039994No Hit
ATGGCGAGGGAGACCCGGGATGGGGAGAAGGTGGTGACAGAGTCCCAGAA7330.40147004858170987No Hit
ATGGCGAGGGTCCTAAATGTTAGGTGTTTCCATCAATGGGATCCACCTTC5240.2869990524649604No Hit
ATGGCGGGGCAGAGAGACTGTGTTACTGGAGCATAGCCAGTATTACCATG4030.22072637050263175No Hit
ATGGCGGGATCTACATGAATCATTGTTTTTGGGTCTCTGTACACTTAATC3950.21634470557950258No Hit
ATGGCGAGGAGGGAGCCATGTTTCCAGCTCCAGTTAAGTCTATTTGAACC3950.21634470557950258No Hit
ATGGCGGGGAAGACCAAACCACTCCATCTGTGATGCTGACGCAGTGAAGG3760.20593825138707078No Hit
ATGGCGGAGGTCGCGCATCTTTTAATATGAAATCCACCCGATAACGATGG3620.19827033777159475No Hit
ATGGCGCGGGGAAAGCAAGTGGTTGGTTCGCGTTGGCGGGTGACAGGTGC3500.191697840386901No Hit
ATGGCGGAGGCTGGAAAAATGTAGAAATGTATGTAAAGAAAGTAAGCCGA3280.1796482618482958No Hit
ATGGCGAGGGAGACCAGTGCCAAGGAGGCCATCAATGTGGAGCAGGCCTT3240.17745742938673123No Hit
ATGGCGGTATTGAGACGAGGAACTACTCGGAAGGGTTGAGCGTCCACACT3240.17745742938673123No Hit
ATGGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3090.16924180765586402No Hit
ATGGCGGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3040.1665032670789083No Hit
ATGGCGGGGGCGCAGACGCATAGAGCAGGGTCTGCTGGCGGACCCGCGGT3030.16595555896351716No Hit
ATGGCGAGGTCAGTCATGGCATGCCCTCTGGATCAGGCCATTGGCCTTCT2990.16376472650195256No Hit
ATGGCGGAGTGTGAAGCTGAATGTAACACACTCAATAGCATTAATTAAAA2980.16321701838656144No Hit
ATGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2860.15664452100186768No Hit
ATGGCGGGGGAAAAGGGCAGAAAGATTCCAGAATATCTGATACATTTTAA2820.1544536885403031No Hit
ATGGCGGGATGTAACAATGTACTTAGTGGGTGATATGGGAGTGGGAAGCT2810.15390598042491196No Hit
ATGGCGGGGATCAAAAGGGACTTTCAGATACTTTGAATCCTTTGGGCAAC2750.1506197317325651No Hit
ATGGCGGAGCTTGTCCGGATGGTGCTGAATGGCTGAGGACATTCTGTATC2740.15007202361717395No Hit
ATGGCGGGGACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2730.14952431550178277No Hit
ATGGCGAGAGGGGGACCAGGCCTTGCTCCACCTTGACCCCTGGAGCTGGG2710.1484288992710005No Hit
ATGGCGGATGACAGTGCCGCTTCTGAATATGGGAAAGCAGCTGTTGCTTT2650.1451426505786536No Hit
ATGGCGAGGTCGGCGCCACATCCCCCTTGGCCCCGAAGATCGGTCCTCTG2650.1451426505786536No Hit
ATGGCGTGGGGCCCTGGAGAAGATTGACCTGAAGTTCATTGACACCACCT2640.1445949424632625No Hit
ATGGCGACCATCCAAAGGATAAACATCATAGGCAGTCGGGAGGGTAGTCG2640.1445949424632625No Hit
ATGGCGGGGACCGAGGCCTGGTGCATGCTGGGACTGAGCTACACTTCTGG2630.14404723434787134No Hit
ATGGCGGATCCTATCTGTTTAAAGCATCCATTTTATACTTTTTGAAGAGG2570.14076098565552445No Hit
ATGGCGGGGAATGCACCTGTTTTATACATGATTGTGTAATTTAAAGGTAT2560.14021327754013332No Hit
ATGGCGGGGCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGC2530.13857015319395988No Hit
ATGGCGAGGATTGCTGCCTTGAAGAAGGGGAGAATGGATTTATCAGGAGT2510.13747473696317758No Hit
ATGGCGGGGGAAGAGCGTCCTCCCTGTTGGGAGCAATCTGTACGTCTGTT2500.13692702884778643No Hit
ATGGCGTCGAGCGTCCGGTTAAAGCCGCTGAATTGTTCGCGTTTACCTTG2450.1341884882708307No Hit
ATGGCGGAGGGAAGCTTGGTTTAGAGGACTTGTTGGGCTCATTTTGGTTT2430.13309307204004842No Hit
ATGGCGGGGAGTCCAGCGAGTATCTGCCCTGCGTGCTGCCACCTGCTGTG2420.13254536392465727No Hit
ATGGCGGGGCTCAGGATGAGGCCGGTCTCACGAAGACATTCCAGACGTGG2360.1292591152323104No Hit
ATGGCGAGGGCGCTCGCTTCCGCGCTGTCAGGCTAGCGCCGCCGTCCCCA2310.12652057465535468No Hit
ATGGCGGGGGTTTTTTTTTTTTTTTTTTTTTTTTTTAAATGTTTTTTTTT2290.12542515842457236No Hit
ATGGCGGGGACCGAAAATGAGATAAAGTCCAGGGATCCCATCAGATGCAG2270.12432974219379009No Hit
ATGGCGGGGGACACTGCCAGCCTACTTCTCTAATGGATTCCTGTGTAGCT2260.12378203407839893No Hit
ATGGCGGGGATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2230.1221389097322255No Hit
ATGGCGGGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2210.1210434935014432No Hit
ATGGCGGGGGCAAGCTTTTTAATCTCTCCAAAGAAGATGATGTCCGCCAG2210.1210434935014432No Hit
ATGGCGGGACGCACAGGTTACATAGGCCGACATCTAGTCCTCCCCTCGTG2190.11994807727066091No Hit
ATGGCGGGCAGCTGCAGAAAGCATAAAATGACAATTAAAAGAAGTTACAT2180.11940036915526978No Hit
ATGGCGGGGGCAAGACAATCTCCAGAAACTGACCTTTAGACAAACCAGCA2180.11940036915526978No Hit
ATGGCGGCATGAGCATCTGGGACGTGAAGAGCCTGGAGTCAGCCTTGAAG2170.11885266103987863No Hit
ATGGCGAGGCTGTAGACCAGGCTGGCCTTGAACTCAGAAATCTGCCTGTC2160.11830495292448748No Hit
ATGGCGAGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACCGACTGCT2140.1172095366937052No Hit
ATGGCGTGGGTACAAGGGTCTCTTTAATGTCTTCTGTGTATGTGCATTTC2130.11666182857831406No Hit
ATGGCGGGGTATAGTTTGCTTGTAAACTTTAATTGCGAAATTTCTTCTTT2130.11666182857831406No Hit
ATGGCGCGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTGC2110.11556641234753175No Hit
ATGGCGCCACCAGCAAGAGCAGAAGCAATACCGCCAGCAATAGCACCAAA2110.11556641234753175No Hit
ATGGCGGGGCTCAGAATCCTTTGCACAAGGAAGGCCTGTGGTCTCCCACT2080.1139232880013583No Hit
ATGGCGAGAAATAAAAGTCTGAAACATGATTAAACTCCTAAGCAGAAAAC2060.11282787177057602No Hit
ATGGCGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2050.11228016365518488No Hit
ATGGCGCGGACCCAGGCTGGCTCAGAGGTGTCTGCCTTATTGGGCAGAAT2050.11228016365518488No Hit
ATGGCGGGGACTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2040.11173245553979373No Hit
ATGGCGGAAGAAACCCTGGACCACCCACCCCAGCAAGGACACTGAGCAAG1990.108993914962838No Hit
ATGGCGCGGAACACATTCTTCAACTCCTCATCACTGTCTTCTTGTGCCAT1990.108993914962838No Hit
ATGGCGGAGATTGTTCTGTGTTCTCCTGCCTCTGAAGGTCATTATGGTAT1980.10844620684744685No Hit
ATGGCGGGATGTATGTATTGTTCCTGGTTTTGTACGGACCATGCCCTCTG1940.10625537438588228No Hit
ATGGCGGAGACCACAGGAAAGGAAACCGCTGACGTGAGTCGCTTCTGCCA1940.10625537438588228No Hit
ATGGCGGGGACAGAGACCAATGACATTTTACCACTTGAAACAATGAATAA1910.10461225003970884No Hit
ATGGCGAGGAGGAGGTCACTGAGCAAGAAAGTCATTCCATCGCTGGGATT1890.10351683380892655No Hit
ATGGCGGTGGTGGCGCACGCCTTTAATCCCAGCACTTGGGAGGCAGAGGC1870.10242141757814427No Hit
ATGGCGAGGATTGCTGCCGTCATGCGGACGGCTTTGGGTGAGGCTTCCTT1860.1018737094627531No Hit
ATGGCGGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1860.1018737094627531No Hit
ATGGCGGGGCACAGAGCTAAAATCTCCAAAAGAACTGGGTGTGAACTCAC1830.10023058511657967No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCTTG302.5159861E-644.00000411
TTAGGTA302.5159861E-644.00000424
AGCAATC302.5159861E-644.00000432
AGCAACG600.044.00000423
ATTAGGT302.5159861E-644.00000423
CGAAATT302.5159861E-644.00000436
GGTCGCG302.5159861E-644.0000049
CTTCCGC302.5159861E-644.00000417
CGCGGAC302.5159861E-644.0000045
GCTGGCG302.5159861E-644.00000434
GCGAAAT302.5159861E-644.00000435
GTTTACC302.5159861E-644.00000441
ATGTGGA302.5159861E-644.00000435
CGGTTAT302.5159861E-644.0000045
CGCATAG302.5159861E-644.00000417
GATCCTC302.5159861E-644.00000425
TGTATCG302.5159861E-644.00000437
GATCAAC302.5159861E-644.0000049
ATAGAGC302.5159861E-644.00000420
TTCCGCG302.5159861E-644.00000418
Produced by FastQC (version 0.11.5)