FastQCFastQC Report
Fri 17 Jun 2016
SRR1527098_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527098_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1322481
Sequences flagged as poor quality0
Sequence length52
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGCGGGGCCTTGGGTTTACGTGGCTCTGCATGTTTGTATCGGTGTCTGTT23770.17973793196272764No Hit
ATGGCGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG23360.17663769838659307No Hit
ATGGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15310.1157672586600488No Hit
ATGGCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14780.11175963964699681No Hit
ATGGCGGGGAACCAGAGCTGAACTAAGGCTGCTCCATCAACACTCCAGGCCC14590.11032294603854423No Hit
ATGGCGGGATCCCAACCTTGAAGTTGTCATGTCATCCTTTTCTGTCTTCCAC14060.10631532702549225No Hit
ATGGCGGGATCTACATGAATCATTGTTTTTGGGTCTCTGTACACTTAATCAA13760.10404686343319866No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATTCG1450.046.00000413
CGAGTTA253.4174373E-546.024
CGACGGA253.4174373E-546.032
CGATTAC301.8616174E-646.012
TAACGGC301.8616174E-646.032
TAGCCGG206.311802E-446.09
ATCGTAC301.8616174E-646.040
AACGGAT253.4174373E-546.035
GCGATTA206.311802E-446.039
TACTCGC253.4174373E-546.043
TTGCCGA206.311802E-446.013
CGCGTTT405.6134013E-946.038
ATCGCGA301.8616174E-646.023
GTATCCG551.8189894E-1245.99999629
CGGGGAT181750.045.5570835
GCGGGGA516350.045.425394
GCGGGGT74600.045.2292254
CGGGGAC110450.044.979635
TGGCGGG1303250.044.978172
GGCGGGA83100.044.975933
Produced by FastQC (version 0.11.5)