##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527098_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1322481
Sequences flagged as poor quality	0
Sequence length	52
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.724749164638283	31.0	30.0	31.0	30.0	31.0
2	30.895389801441382	31.0	30.0	31.0	30.0	31.0
3	30.92113383859579	31.0	30.0	31.0	30.0	31.0
4	35.16008547570816	35.0	35.0	35.0	35.0	37.0
5	35.10509186899471	35.0	35.0	35.0	35.0	37.0
6	35.15661548256648	35.0	35.0	35.0	35.0	37.0
7	35.197080336125815	35.0	35.0	35.0	35.0	37.0
8	35.08234220378213	35.0	35.0	35.0	35.0	37.0
9	36.595911774913965	37.0	35.0	39.0	35.0	39.0
10	32.28624002915732	35.0	30.0	37.0	25.0	39.0
11	29.796477983426605	32.0	26.0	35.0	20.0	37.0
12	29.199862228644495	32.0	25.0	35.0	18.0	37.0
13	29.259454011059518	32.0	25.0	35.0	18.0	37.0
14	29.719057589485217	31.0	26.0	35.0	20.0	38.0
15	29.50356564668982	31.0	26.0	35.0	19.0	38.0
16	26.25872810270998	28.0	22.0	33.0	9.0	37.0
17	26.912694397877928	29.0	22.0	33.0	12.0	37.0
18	27.430399378138514	29.0	23.0	33.0	14.0	37.0
19	27.998483910165817	29.0	23.0	34.0	18.0	37.0
20	28.18835431284079	29.0	23.0	34.0	18.0	37.0
21	28.080086594816862	29.0	23.0	34.0	18.0	37.0
22	28.214410641816404	29.0	23.0	34.0	18.0	37.0
23	28.313139470434734	30.0	24.0	34.0	18.0	37.0
24	28.04486264831026	29.0	23.0	34.0	18.0	36.0
25	28.098228254318965	29.0	23.0	33.0	18.0	36.0
26	27.150488362403696	29.0	22.0	33.0	15.0	36.0
27	26.996697873164152	29.0	22.0	33.0	14.0	36.0
28	26.639261358008167	28.0	22.0	33.0	13.0	36.0
29	26.325581237083934	28.0	22.0	33.0	12.0	35.0
30	25.854027392454032	28.0	21.0	32.0	11.0	35.0
31	25.65901513896986	28.0	21.0	32.0	11.0	35.0
32	25.516301557451488	28.0	21.0	32.0	10.0	35.0
33	25.00605226086424	26.0	20.0	32.0	9.0	34.0
34	24.98105001130451	26.0	20.0	31.0	9.0	34.0
35	24.917548153810905	26.0	20.0	31.0	9.0	34.0
36	24.62094653911852	26.0	20.0	31.0	9.0	34.0
37	24.369721757817313	26.0	18.0	31.0	9.0	34.0
38	24.2599795384584	26.0	18.0	31.0	9.0	34.0
39	23.909188109318773	26.0	18.0	31.0	8.0	34.0
40	23.902664008027337	26.0	18.0	31.0	8.0	34.0
41	23.744950589082187	26.0	18.0	31.0	8.0	34.0
42	23.597175309134876	26.0	18.0	31.0	8.0	34.0
43	23.652324683681655	26.0	18.0	31.0	8.0	34.0
44	24.919972385236537	26.0	20.0	31.0	10.0	34.0
45	25.613941523545517	28.0	20.0	32.0	11.0	34.0
46	25.965666803530638	29.0	22.0	32.0	12.0	34.0
47	25.771214860553762	29.0	21.0	32.0	10.0	34.0
48	25.939651306899684	29.0	22.0	32.0	11.0	34.0
49	26.013121549572357	29.0	22.0	32.0	11.0	34.0
50	25.694090123033902	29.0	20.0	32.0	10.0	34.0
51	25.854363881220223	29.0	21.0	32.0	10.0	34.0
52	25.463695130591667	28.0	20.0	32.0	10.0	34.0
>>END_MODULE
>>Per sequence quality scores	warn
#Quality	Count
12	11.0
13	44.0
14	166.0
15	481.0
16	1402.0
17	3494.0
18	7153.0
19	13423.0
20	23189.0
21	37230.0
22	54982.0
23	74209.0
24	96100.0
25	116985.0
26	134299.0
27	143545.0
28	139810.0
29	121566.0
30	97408.0
31	74227.0
32	56599.0
33	42172.0
34	28647.0
35	20096.0
36	11642.0
37	10417.0
38	11177.0
39	2007.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	0.0	100.0	0.0
3	100.0	0.0	0.0	0.0
4	100.0	0.0	0.0	0.0
5	0.0	0.0	0.0	100.0
6	100.0	0.0	0.0	0.0
7	98.36700867536094	1.3669761607161086	0.12446303576384085	0.1415521281591191
8	98.04042553352373	1.473140256835448	0.2685104738744829	0.21792373576633617
9	91.53379141174807	6.317595489084531	0.45543187387947354	1.6931812252879246
10	38.63783298209955	41.118095458460274	9.10992294029177	11.134148619148403
11	31.140182732303906	24.052519469088782	26.795545644890172	18.011752153717143
12	20.06592155199205	12.789824579710407	33.77954012193748	33.36471374636006
13	21.867006028820075	26.287334184763335	30.390909207769333	21.454750578647257
14	29.568742386469065	27.486292808743567	19.599827899228796	23.345136905558565
15	30.285803727993066	25.303199062973302	20.70192312781809	23.709074081215533
16	31.429260609415184	32.205906927963426	20.309176464539	16.05565599808239
17	29.262575416962513	27.444326232286137	22.223306043716317	21.069792307035033
18	24.49547479321064	27.639036023958003	24.87060305592292	22.99488612690844
19	26.79131117951789	27.477218954374393	24.40360201772275	21.32786784838497
20	26.65830359755641	26.91259836625252	25.64762745173655	20.781470584454524
21	25.06130522858173	26.0058178529597	26.888930729439593	22.043946189018975
22	26.021923944464987	24.930641725665623	25.710766355055387	23.336667974814006
23	22.5152573080445	26.5404947216633	28.04274692793318	22.901501042359023
24	22.416352295420502	28.51655335691023	26.38714658282425	22.679947764845014
25	24.457969528484718	31.323852667826607	24.81071561708637	19.407462186602302
26	23.498636275303767	30.742445449121764	23.66378042482274	22.09513785075173
27	23.515725367699044	26.87494187062045	25.64407352544195	23.965259236238552
28	24.174335963994945	26.912220288987136	25.46546982527537	23.447973921742545
29	24.63740499863514	26.132549352315838	25.428569484174062	23.801476164874956
30	25.128224904554397	25.629555358451274	26.0706959116993	23.171523825295033
31	26.052472587507875	26.477280202891386	25.152195003179628	22.318052206421115
32	25.06818623481169	27.646446338359493	26.07553530069619	21.209832126132625
33	26.029409874319555	25.95666780846001	26.030619721568776	21.983302595651658
34	23.4808666438308	28.18656751968459	26.542082646177906	21.790483190306702
35	23.463626320529368	28.487592638381948	26.96227771892375	21.086503322164933
36	24.23694555914225	27.784066462958634	25.79205296711257	22.18693501078654
37	24.38121984361212	27.849927522588224	24.880054987557475	22.888797646242175
38	25.09540779791921	28.525778442185555	24.929809955681783	21.449003804213447
39	24.791811753817257	27.01914053963724	25.640822060959668	22.548225645585834
40	23.747335500472218	28.0202135229164	25.058885534083288	23.173565442528098
41	22.827624744703325	28.483811865728125	26.291644265588694	22.396919123979853
42	22.06957982761189	28.04252008157395	25.340931174058458	24.546968916755706
43	22.914582515741248	27.830116273882194	26.108654869143678	23.14664634123288
44	22.81393834769649	27.750568817245767	25.167696171060307	24.26779666399744
45	22.479113121473958	28.132956163453386	26.09693447391683	23.290996241155828
46	24.072255102341735	27.21437963948064	24.754760181809797	23.958605076367828
47	22.32213544088724	28.47579662770202	25.670690164924864	23.53137776648587
48	23.843745203144696	28.082898733516775	25.213519135624633	22.859836927713893
49	23.130162172462214	27.901346030680212	25.622523121315165	23.34596867554241
50	22.73741550918312	28.526307750357095	25.77224171840654	22.96403502205325
51	22.7207801095063	28.231483098812006	26.26956455329037	22.778172238391324
52	22.97628472545163	28.147549945897143	25.268567185464292	23.607598143186934
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	1.0
9	1.0
10	1.0
11	150.5
12	300.0
13	1339.5
14	5606.0
15	8833.0
16	10904.0
17	12975.0
18	11873.0
19	10771.0
20	9223.0
21	7675.0
22	6862.0
23	6049.0
24	5875.0
25	5701.0
26	7436.5
27	9172.0
28	10146.0
29	11120.0
30	12499.0
31	13878.0
32	17122.0
33	20366.0
34	22931.5
35	25497.0
36	27144.5
37	28792.0
38	32582.5
39	41403.0
40	46433.0
41	44716.0
42	42999.0
43	45425.5
44	47852.0
45	52884.0
46	57916.0
47	61841.5
48	65767.0
49	67512.5
50	69258.0
51	71469.5
52	73681.0
53	78588.5
54	83496.0
55	85191.0
56	86886.0
57	86920.5
58	86955.0
59	89024.5
60	91094.0
61	87697.5
62	84301.0
63	79650.0
64	68714.5
65	62430.0
66	55813.0
67	49196.0
68	44446.0
69	39696.0
70	31425.5
71	23155.0
72	17873.5
73	12592.0
74	10649.5
75	8707.0
76	7207.0
77	5707.0
78	4880.5
79	4054.0
80	3208.5
81	2363.0
82	1856.5
83	1350.0
84	1013.5
85	677.0
86	549.5
87	422.0
88	288.5
89	146.0
90	137.0
91	128.5
92	120.0
93	117.5
94	115.0
95	100.0
96	85.0
97	42.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1322481.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.58082870368189
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.48744706708717	13.997834554841601
2	10.207338825621113	3.9973630612985698
3	4.256126118206345	2.5001542938559496
4	2.2476545043205576	1.7604375133663943
5	1.499691292916604	1.4682599157501623
6	1.0389230080099134	1.2205784073693624
7	0.782672522387228	1.072776361436006
8	0.585887286529216	0.9177726877754854
9	0.5108150219777655	0.9001963300152712
>10	5.25936367536054	23.7812586044927
>50	1.313659604264464	18.18382790017997
>100	0.7854915408239058	26.16956488425436
>500	0.01943658465728868	2.466600815971632
>1k	0.0054929478379294105	1.5633746693925457
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATGGCGGGGCCTTGGGTTTACGTGGCTCTGCATGTTTGTATCGGTGTCTGTT	2377	0.17973793196272764	No Hit
ATGGCGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	2336	0.17663769838659307	No Hit
ATGGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1531	0.1157672586600488	No Hit
ATGGCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1478	0.11175963964699681	No Hit
ATGGCGGGGAACCAGAGCTGAACTAAGGCTGCTCCATCAACACTCCAGGCCC	1459	0.11032294603854423	No Hit
ATGGCGGGATCCCAACCTTGAAGTTGTCATGTCATCCTTTTCTGTCTTCCAC	1406	0.10631532702549225	No Hit
ATGGCGGGATCTACATGAATCATTGTTTTTGGGTCTCTGTACACTTAATCAA	1376	0.10404686343319866	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	6.049236246116202E-4	0.0	0.0	0.0	0.0
8	6.805390776880726E-4	0.0	0.0	0.0	0.0
9	0.0013610781553761453	0.0	0.0	0.0	0.0
10	0.004763773543816508	0.0	0.0	0.0	0.0
11	0.007259083495339442	0.0	0.0	0.0	0.0
12	0.00869577710379204	0.0	0.0	0.0	0.0
13	0.013383935194532095	0.0	0.0	0.0	0.0
14	0.020869865049100895	0.0	0.0	0.0	0.0
15	0.028582641262899052	0.0	0.0	0.0	0.0
16	0.03432941569670944	0.0	0.0	0.0	0.0
17	0.037202802913614635	0.0	0.0	0.0	0.0
18	0.040076190130519836	0.0	0.0	0.0	0.0
19	0.045596118205100865	0.0	0.0	0.0	0.0
20	0.04854512087508252	0.0	0.0	0.0	0.0
21	0.05202343171659933	0.0	0.0	0.0	0.0
22	0.05580420437042196	0.0	0.0	0.0	0.0
23	0.0586019761342507	0.0	0.0	0.0	0.0
24	0.061172901538850084	0.0	0.0	0.0	0.0
25	0.0643487505680611	0.0	0.0	0.0	0.0
26	0.06699529142573693	0.0	0.0	0.0	0.0
27	0.06994429409571858	0.0	0.0	0.0	0.0
28	0.07334698948415894	0.0	0.0	0.0	0.0
29	0.07591791488875832	0.0	0.0	0.0	0.0
30	0.07848884029335772	0.0	0.0	0.0	0.0
31	0.08151345841641582	0.0	0.0	0.0	0.0
32	0.08544546197639134	0.0	0.0	0.0	0.0
33	0.08801638738099073	0.0	0.0	0.0	0.0
34	0.09255331456557787	0.0	0.0	0.0	0.0
35	0.09565354814171244	0.0	0.0	0.0	0.0
36	0.09875378171784699	0.0	0.0	0.0	0.0
37	0.10298824709012833	0.0	0.0	0.0	0.0
38	0.1069958661031803	0.0	0.0	0.0	0.0
39	0.11070102330392648	0.0	0.0	0.0	0.0
40	0.11538918139466654	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATATTCG	145	0.0	46.000004	13
CGAGTTA	25	3.4174373E-5	46.0	24
CGACGGA	25	3.4174373E-5	46.0	32
CGATTAC	30	1.8616174E-6	46.0	12
TAACGGC	30	1.8616174E-6	46.0	32
TAGCCGG	20	6.311802E-4	46.0	9
ATCGTAC	30	1.8616174E-6	46.0	40
AACGGAT	25	3.4174373E-5	46.0	35
GCGATTA	20	6.311802E-4	46.0	39
TACTCGC	25	3.4174373E-5	46.0	43
TTGCCGA	20	6.311802E-4	46.0	13
CGCGTTT	40	5.6134013E-9	46.0	38
ATCGCGA	30	1.8616174E-6	46.0	23
GTATCCG	55	1.8189894E-12	45.999996	29
CGGGGAT	18175	0.0	45.557083	5
GCGGGGA	51635	0.0	45.42539	4
GCGGGGT	7460	0.0	45.229225	4
CGGGGAC	11045	0.0	44.97963	5
TGGCGGG	130325	0.0	44.97817	2
GGCGGGA	8310	0.0	44.97593	3
>>END_MODULE