FastQCFastQC Report
Fri 17 Jun 2016
SRR1527097_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527097_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1362965
Sequences flagged as poor quality0
Sequence length52
%GC52

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGCGGGGCCTTGGGTTTACGTGGCTCTGCATGTTTGTATCGGTGTCTGTT22960.16845626997024868No Hit
ATGGCGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG22880.16786931432575303No Hit
ATGGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15850.11629058706569867No Hit
ATGGCGGGATCTACATGAATCATTGTTTTTGGGTCTCTGTACACTTAATCAA14160.10389114907572829No Hit
ATGGCGGGATCCCAACCTTGAAGTTGTCATGTCATCCTTTTCTGTCTTCCAC13730.10073626248656423No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACTA351.01976184E-746.00000430
ATAATCG206.31187E-446.037
TATTACG206.31187E-446.038
TACGTAA206.31187E-446.046
CGTTAGC650.045.99999623
CGTAACC650.045.99999631
CGCAATC650.045.99999646
CGGGGAT192350.045.4619145
GCGGGGA536950.045.2975164
GCGGGGT77750.045.112544
TGGCGGG1349700.044.9502872
ATGGCGG1374650.044.885681
GGCGGGA83900.044.79383
CGGGGAG140650.044.773555
CGGGGAC107800.044.7625245
GGCGGGG1251700.044.621883
GCGGGAT38800.044.518044
CGGGGGA156300.044.2783135
CGGGATA4850.044.1030925
ATATTCG3050.043.73770513