FastQCFastQC Report
Fri 17 Jun 2016
SRR1527093_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527093_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2343431
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC177750.7585032373472913No Hit
GATCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG144160.6151663949141237No Hit
GATCTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT94250.40218807381143284No Hit
GATCTTGGGGAGCCAGTAGACTGGTTCTTCACTTTACCTACCGTATGCTA82910.3537974875300361No Hit
GATCTTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC53340.22761497991619978No Hit
GATCTTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT52870.225609373606477No Hit
GATCTTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT52120.2224089380058555No Hit
GATCTTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG47620.2032063244021266No Hit
GATCTTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC47310.20188347768720308No Hit
GATCTTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT42610.18182741458997512No Hit
GATCTTGGGGGGGCTGGAGAGATGGCTCAGGGGTTAAGAGCACTGACTGC42500.18135801736855064No Hit
GATCTTGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGC41980.17913904868545308No Hit
GATCTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC41380.1765787002049559No Hit
GATCTTGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCC38010.16219807623949672No Hit
GATCTTGGGACATGAATCATTGTTTTTGGGTCTCTGTACACTTAATCAAT37400.15959505528432455No Hit
GATCTTGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC36840.15720539670252717No Hit
GATCTTGGGATTTCTCTTCTATATCAGGCTCTTGTTAATTAGTCTTTTTT35250.15042047322920965No Hit
GATCTTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG35070.1496523686850605No Hit
GATCTTGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCT32500.13868554269359756No Hit
GATCTTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC30780.13134587704950562No Hit
GATCTTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA29420.125542420493712No Hit
GATCTTGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTC29050.12396353893073873No Hit
GATCTTGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAA28490.12157388034894136No Hit
GATCTTGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTA27940.11922689424181893No Hit
GATCTTGGGGGGCTGGAGAGATGGCTCAGCGGGTAAGAGCACTGACTGCT27560.11760534020417072No Hit
GATCTTGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTT26960.11504499172367354No Hit
GATCTTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC26930.11491697429964869No Hit
GATCTTGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA26850.11457559450224904No Hit
GATCTTGGGATGTACATACTGACTGTCAATACAGCCTCCTTGCTATGTCC25530.10894282784515526No Hit
GATCTTGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGT25430.10851610309840572No Hit
GATCTTGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTGC24150.10305402634001172No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGAT207.8581594E-444.029
CGATTAT207.8581594E-444.012
CGATTAC302.5288482E-644.017
CGATTAA207.8581594E-444.030
ATCGTAC207.8581594E-444.025
TGCGATA207.8581594E-444.027
GTCAATT302.5288482E-644.029
CGAATAC254.4442597E-544.029
CGTACAA351.4469515E-744.022
ACGTAAT408.3182385E-944.038
CGTTTAA351.4469515E-744.030
CGATAGT207.8581594E-444.037
GCGTAAT207.8581594E-444.014
GCGTAAC302.5288482E-644.034
TCAACCG351.4469515E-744.034
TAGTACG207.8581594E-444.037
CCGTATG10050.042.90547641
TTGGGAT284850.042.5325625
ACCGTAT10050.042.46766340
GATCTTG2441500.042.1167341
Produced by FastQC (version 0.11.5)