##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527093_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2343431
Sequences flagged as poor quality	0
Sequence length	50
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.52001787123239	33.0	31.0	34.0	31.0	34.0
2	32.041138825935136	33.0	31.0	34.0	30.0	34.0
3	32.73411975859327	34.0	31.0	34.0	31.0	34.0
4	36.35792690290433	37.0	37.0	37.0	35.0	37.0
5	36.368721332098104	37.0	37.0	37.0	35.0	37.0
6	36.4887594300835	37.0	37.0	37.0	35.0	37.0
7	36.527831628070125	37.0	37.0	37.0	35.0	37.0
8	36.67448412178553	37.0	37.0	37.0	35.0	37.0
9	38.766415140876774	39.0	39.0	39.0	39.0	39.0
10	38.27649373930788	39.0	39.0	39.0	37.0	39.0
11	38.06027529720312	39.0	38.0	39.0	37.0	39.0
12	37.56777434454012	39.0	37.0	39.0	35.0	39.0
13	37.47148902613305	39.0	37.0	39.0	35.0	39.0
14	38.28473806141508	40.0	38.0	40.0	35.0	40.0
15	38.24608490712976	40.0	38.0	40.0	35.0	40.0
16	38.084654935434415	40.0	38.0	40.0	35.0	40.0
17	38.122259627016966	40.0	38.0	40.0	34.0	40.0
18	38.06400657838869	40.0	38.0	40.0	34.0	40.0
19	38.03111932888146	40.0	38.0	40.0	34.0	40.0
20	37.81016680243626	40.0	38.0	40.0	33.0	40.0
21	38.01914244541444	40.0	38.0	40.0	34.0	40.0
22	37.86023356352288	40.0	38.0	40.0	34.0	40.0
23	37.945171417464394	40.0	38.0	40.0	34.0	40.0
24	38.00466836872944	40.0	37.0	40.0	34.0	40.0
25	37.97402270431687	40.0	37.0	40.0	34.0	40.0
26	37.87466368755897	40.0	37.0	40.0	34.0	40.0
27	37.779400374920364	40.0	37.0	40.0	34.0	40.0
28	37.60998126251637	40.0	37.0	40.0	34.0	40.0
29	37.824185137091725	40.0	37.0	40.0	34.0	40.0
30	37.846802402118946	40.0	37.0	40.0	34.0	40.0
31	37.833605939325714	40.0	37.0	40.0	34.0	40.0
32	37.135323805138704	40.0	36.0	40.0	33.0	40.0
33	37.341679358171845	40.0	36.0	40.0	33.0	40.0
34	37.27566973382191	40.0	36.0	40.0	33.0	40.0
35	37.31205228572977	40.0	36.0	40.0	33.0	40.0
36	37.2000524017989	40.0	36.0	40.0	33.0	40.0
37	37.194670549292894	40.0	35.0	40.0	33.0	40.0
38	37.038414615151886	40.0	35.0	40.0	33.0	40.0
39	37.02615523990252	40.0	35.0	40.0	33.0	40.0
40	36.925208807086705	40.0	35.0	40.0	32.0	40.0
41	36.83980027574953	40.0	35.0	40.0	32.0	40.0
42	36.73017511503432	39.0	35.0	40.0	32.0	40.0
43	36.68027989729589	39.0	35.0	40.0	32.0	40.0
44	36.53447786600075	39.0	35.0	40.0	32.0	40.0
45	36.45446697598521	39.0	35.0	40.0	31.0	40.0
46	36.14227131074053	39.0	35.0	40.0	31.0	40.0
47	36.08542389342806	38.0	35.0	40.0	31.0	40.0
48	35.973982165465934	38.0	35.0	40.0	30.0	40.0
49	35.67172406612356	38.0	35.0	40.0	30.0	40.0
50	35.30658850207239	37.0	34.0	40.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	0.0
14	3.0
15	4.0
16	48.0
17	91.0
18	237.0
19	496.0
20	883.0
21	1508.0
22	2263.0
23	3335.0
24	5358.0
25	7329.0
26	9283.0
27	12929.0
28	17512.0
29	22554.0
30	30443.0
31	39309.0
32	50879.0
33	70447.0
34	141665.0
35	176709.0
36	176228.0
37	332269.0
38	762251.0
39	479396.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	0.0	100.0	0.0
4	0.0	0.0	0.0	100.0
5	0.0	0.0	100.0	0.0
6	0.0	0.0	100.0	0.0
7	99.76423457742088	0.1629235083089709	0.06119232868388273	0.011649585586262194
8	99.62682920896754	0.24664690362122885	0.0902522839375258	0.036271603473710126
9	97.89138233641187	1.2557655847345197	0.2744693571092983	0.5783827217443143
10	52.16931925881325	35.84073949691713	4.404055421303209	7.585885822966411
11	34.57379372381777	21.58224415397765	24.2900260344768	19.55393608772778
12	30.651766576442828	16.223392111822367	26.81615119028467	26.30869012145013
13	25.2924878095408	16.440979060189953	30.664568318845316	27.601964811423933
14	25.745968197911523	17.840721574477765	30.454321036121822	25.95898919148889
15	27.443607257905185	18.583350651245972	29.373085872807863	24.599956218040983
16	32.15383768500118	20.07180924038301	24.890043700881314	22.884309373734492
17	31.802216493679563	19.336861209056295	24.100048177223908	24.76087412004023
18	28.95852278134069	20.560153040563176	24.747987032688396	25.733337145407738
19	29.088161759403196	21.71661977672908	24.103803354995303	25.091415108872418
20	28.075458590417213	22.87300116794563	24.878522132719077	24.17301810891808
21	30.37704118448548	20.840255164329566	25.63856157915467	23.144142072030284
22	29.710497130062713	20.22956937925631	26.142054107844437	23.91787938283653
23	29.661807836458593	21.116900817647288	25.079680178336805	24.14161116755731
24	31.057069740905536	19.459203193949385	24.294976041539094	25.188751023605988
25	28.72967029965892	19.729149268743136	24.22490783812282	27.316272593475123
26	27.135213283429298	20.402990316335323	24.82650438609031	27.635292014145072
27	26.68207427485597	18.593805407541335	24.888678181691716	29.835442135910977
28	27.30479369778756	22.09183884654594	23.33262639266955	27.270741062996944
29	29.341508241548397	21.969198154330126	22.706919896510712	25.982373707610762
30	29.233120155874015	20.116060596620937	23.380206201932126	27.270613045572922
31	30.46490380984121	21.654445981127672	23.000079797527643	24.880570411503474
32	33.13282959899395	20.18326974423399	23.097373039786532	23.586527616985524
33	32.37219273791291	19.839798995575293	24.729296488780765	23.058711777731027
34	27.970569647666178	22.62763443856465	26.69056609731629	22.711229816452885
35	26.34419362037969	24.98400849011556	26.725173474277675	21.946624415227074
36	27.725501625607922	26.803776172628936	22.384913402613517	23.085808799149625
37	28.690624985331336	26.401161374070753	21.432293077969867	23.475920562628044
38	29.884686171685875	26.482879163073285	20.93916996062611	22.69326470461473
39	28.867161013061615	23.878663378610252	21.95003821320107	25.304137395127057
40	28.1821397771046	23.666837214323785	22.0839017662564	26.06712124231522
41	25.209660536196715	24.277992396618462	22.11748500382559	28.394862063359238
42	24.6760839128611	22.387815130891415	23.653267367377147	29.28283358887034
43	24.96847570933388	21.484396169547985	24.864098836278945	28.683029284839197
44	25.98459267629386	20.76937618389447	25.22813771773097	28.017893422080704
45	27.088572268609575	22.28996714646175	22.987534089973206	27.633926494955475
46	26.805312381717233	23.52388442416269	23.325500089398833	26.34530310472124
47	26.045102245382946	22.070459936733787	25.02783312160674	26.856604696276527
48	27.6343958921769	21.881079494126347	25.432922923696065	25.051601690000684
49	27.93314588737624	22.12380053007748	24.795694859374994	25.14735872317128
50	25.64086589278711	21.607378241561197	25.584708916114874	27.16704694953681
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	1.0
5	3.0
6	5.0
7	55.5
8	106.0
9	732.5
10	1359.0
11	2358.5
12	3358.0
13	3964.0
14	4570.0
15	3848.0
16	3126.0
17	3261.5
18	3397.0
19	4186.5
20	4976.0
21	4902.0
22	4828.0
23	7869.0
24	10910.0
25	10509.5
26	10109.0
27	11719.0
28	13329.0
29	16559.5
30	19790.0
31	24500.5
32	29211.0
33	30347.5
34	31484.0
35	34946.5
36	38409.0
37	40907.5
38	43406.0
39	49744.0
40	56082.0
41	58725.5
42	61369.0
43	71055.5
44	80742.0
45	85372.5
46	90003.0
47	99533.0
48	109063.0
49	113616.0
50	118169.0
51	124127.0
52	130085.0
53	143569.5
54	157054.0
55	182470.5
56	207887.0
57	219024.0
58	230161.0
59	232374.5
60	234588.0
61	231733.5
62	228879.0
63	189918.5
64	150958.0
65	121499.5
66	92041.0
67	75216.5
68	58392.0
69	50801.5
70	43211.0
71	36233.0
72	29255.0
73	28099.0
74	26943.0
75	18461.5
76	9980.0
77	6728.0
78	3476.0
79	2737.5
80	1999.0
81	1260.0
82	521.0
83	336.5
84	152.0
85	96.5
86	41.0
87	23.0
88	5.0
89	3.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2343431.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	7.132877666670637
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.84783001795977	4.696845161338295
2	11.353925864897136	1.6197232846151772
3	4.97208402815801	1.0639580136377413
4	2.679342455803829	0.7644568785746234
5	1.585749551514133	0.5655478780464069
6	0.9211778327166709	0.394238927401008
7	0.5904345288246652	0.29480480849992585
8	0.42531444246009215	0.24269727103488517
9	0.31481393217902703	0.20209763393968916
>10	4.172679293171595	7.951354282904585
>50	3.365400962552792	17.630220201339704
>100	3.5508414237546804	45.55016522100778
>500	0.15109963505339066	7.288877966851305
>1k	0.06493538035352325	8.807204931240685
>5k	0.0031218932862270795	1.4941438263610027
>10k+	0.001248757314490832	1.4336637132071608
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATCTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	17775	0.7585032373472913	No Hit
GATCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	14416	0.6151663949141237	No Hit
GATCTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	9425	0.40218807381143284	No Hit
GATCTTGGGGAGCCAGTAGACTGGTTCTTCACTTTACCTACCGTATGCTA	8291	0.3537974875300361	No Hit
GATCTTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	5334	0.22761497991619978	No Hit
GATCTTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT	5287	0.225609373606477	No Hit
GATCTTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	5212	0.2224089380058555	No Hit
GATCTTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG	4762	0.2032063244021266	No Hit
GATCTTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	4731	0.20188347768720308	No Hit
GATCTTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	4261	0.18182741458997512	No Hit
GATCTTGGGGGGGCTGGAGAGATGGCTCAGGGGTTAAGAGCACTGACTGC	4250	0.18135801736855064	No Hit
GATCTTGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGC	4198	0.17913904868545308	No Hit
GATCTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	4138	0.1765787002049559	No Hit
GATCTTGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCC	3801	0.16219807623949672	No Hit
GATCTTGGGACATGAATCATTGTTTTTGGGTCTCTGTACACTTAATCAAT	3740	0.15959505528432455	No Hit
GATCTTGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC	3684	0.15720539670252717	No Hit
GATCTTGGGATTTCTCTTCTATATCAGGCTCTTGTTAATTAGTCTTTTTT	3525	0.15042047322920965	No Hit
GATCTTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG	3507	0.1496523686850605	No Hit
GATCTTGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCT	3250	0.13868554269359756	No Hit
GATCTTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC	3078	0.13134587704950562	No Hit
GATCTTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	2942	0.125542420493712	No Hit
GATCTTGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTC	2905	0.12396353893073873	No Hit
GATCTTGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAA	2849	0.12157388034894136	No Hit
GATCTTGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTA	2794	0.11922689424181893	No Hit
GATCTTGGGGGGCTGGAGAGATGGCTCAGCGGGTAAGAGCACTGACTGCT	2756	0.11760534020417072	No Hit
GATCTTGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTT	2696	0.11504499172367354	No Hit
GATCTTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	2693	0.11491697429964869	No Hit
GATCTTGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA	2685	0.11457559450224904	No Hit
GATCTTGGGATGTACATACTGACTGTCAATACAGCCTCCTTGCTATGTCC	2553	0.10894282784515526	No Hit
GATCTTGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGT	2543	0.10851610309840572	No Hit
GATCTTGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTGC	2415	0.10305402634001172	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	4.267247467495309E-5	0.0	0.0	0.0	0.0
8	1.7068989869981237E-4	0.0	0.0	0.0	0.0
9	3.8405227207457783E-4	0.0	0.0	0.0	0.0
10	0.007467683068116791	0.0	0.0	0.0	0.0
11	0.013228467149235458	0.0	0.0	0.0	0.0
12	0.017581059566080675	0.0	0.0	0.0	0.0
13	0.023043136324474668	0.0	0.0	0.0	0.0
14	0.03008409464584193	0.0	0.0	0.0	0.0
15	0.03772246761265853	0.0	0.0	0.0	0.0
16	0.04617161759829925	0.0	0.0	0.0	0.0
17	0.05201774662876782	0.0	0.0	0.0	0.0
18	0.0585893077287106	0.0	0.0	0.0	0.0
19	0.06853199432797466	0.0	0.0	0.0	0.0
20	0.07702381678829033	0.0	0.0	0.0	0.0
21	0.08363805036290806	0.0	0.0	0.0	0.0
22	0.0910630609563499	0.0	0.0	0.0	0.0
23	0.09951221094199061	0.0	0.0	0.0	0.0
24	0.10710791143413226	0.0	0.0	0.0	0.0
25	0.1131674028379756	0.0	0.0	0.0	0.0
26	0.11969629146324343	0.0	0.0	0.0	0.0
27	0.12793207907550938	0.0	0.0	0.0	0.0
28	0.13868554269359756	0.0	0.0	0.0	0.0
29	0.1461105532870394	0.0	0.0	0.0	0.0
30	0.15323685655775657	0.0	0.0	0.0	0.0
31	0.16078988457522325	0.0	0.0	0.0	0.0
32	0.17060455375046246	0.0	0.0	0.0	0.0
33	0.1794804284828527	0.0	0.0	0.0	0.0
34	0.1878442335191435	0.0	0.0	0.0	0.0
35	0.20662012237612287	0.0	0.0	0.0	0.0
36	0.22010462437340805	0.0	0.0	0.0	0.0
37	0.23226627965576968	0.0	0.0	0.0	0.0
38	0.24233698367905862	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCGAT	20	7.8581594E-4	44.0	29
CGATTAT	20	7.8581594E-4	44.0	12
CGATTAC	30	2.5288482E-6	44.0	17
CGATTAA	20	7.8581594E-4	44.0	30
ATCGTAC	20	7.8581594E-4	44.0	25
TGCGATA	20	7.8581594E-4	44.0	27
GTCAATT	30	2.5288482E-6	44.0	29
CGAATAC	25	4.4442597E-5	44.0	29
CGTACAA	35	1.4469515E-7	44.0	22
ACGTAAT	40	8.3182385E-9	44.0	38
CGTTTAA	35	1.4469515E-7	44.0	30
CGATAGT	20	7.8581594E-4	44.0	37
GCGTAAT	20	7.8581594E-4	44.0	14
GCGTAAC	30	2.5288482E-6	44.0	34
TCAACCG	35	1.4469515E-7	44.0	34
TAGTACG	20	7.8581594E-4	44.0	37
CCGTATG	1005	0.0	42.905476	41
TTGGGAT	28485	0.0	42.532562	5
ACCGTAT	1005	0.0	42.467663	40
GATCTTG	244150	0.0	42.116734	1
>>END_MODULE