FastQCFastQC Report
Fri 17 Jun 2016
SRR1527092_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527092_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2484715
Sequences flagged as poor quality0
Sequence length52
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC166740.6710628784387747No Hit
GATCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT132970.5351519188317372No Hit
GATCTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC88470.35605693208275396No Hit
GATCTTGGGGAGCCAGTAGACTGGTTCTTCACTTTACCTACCGTATGCTAAG81400.3276029645251065No Hit
GATCTTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT49160.19784965277707908No Hit
GATCTTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT47730.1920944655624488No Hit
GATCTTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT47050.1893577331806666No Hit
GATCTTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC44740.18006089229549466No Hit
GATCTTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC43830.17639850043163904No Hit
GATCTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA41150.16561255516226206No Hit
GATCTTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG40970.16488812600237854No Hit
GATCTTGGGACATGAATCATTGTTTTTGGGTCTCTGTACACTTAATCAATTT36980.148829946291627No Hit
GATCTTGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTC36900.1485079777761232No Hit
GATCTTGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT35740.14383943430131826No Hit
GATCTTGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG35420.1425515602393031No Hit
GATCTTGGGGGGGCTGGAGAGATGGCTCAGGGGTTAAGAGCACTGACTGCTC35050.14106245585509808No Hit
GATCTTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG34610.13929162901982722No Hit
GATCTTGGGATTTCTCTTCTATATCAGGCTCTTGTTAATTAGTCTTTTTTGT33800.13603169780035135No Hit
GATCTTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT30140.12130163821605294No Hit
GATCTTGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA29120.11719653964337963No Hit
GATCTTGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACT28430.11441956119715943No Hit
GATCTTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC28230.11361463990839996No Hit
GATCTTGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC27480.11059618507555194No Hit
GATCTTGGGGGGCTGGAGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCT26680.10737649992051401No Hit
GATCTTGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACG26310.10588739553630899No Hit
GATCTTGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG25740.10359336986334448No Hit
GATCTTGGGATGTACATACTGACTGTCAATACAGCCTCCTTGCTATGTCCAC25460.10246648005908121No Hit
GATCTTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC25230.10154082057700782No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAG206.312878E-446.032
ACGTTAT206.312878E-446.037
CGCATAA206.312878E-446.027
CACGCTA301.8622832E-646.032
GCGCATA206.312878E-446.015
CGAATAC253.4183104E-546.029
TTACGAA206.312878E-446.035
CACGTAA253.4183104E-546.038
CGGTAAT405.6152203E-946.027
ATATCGT551.8189894E-1245.99999633
GCGTAAC351.0202166E-745.99999634
ACCGTAT10900.045.15596440
TTGGGAT296750.044.1165965
GATCTTG2595350.043.9413571
CCGTATG11150.043.9372241
ATCTTGG2587350.043.9154322
TCTTGGG2535250.043.8983
CTTGGGG1340600.043.75254
TTGGGAC156650.043.6508145
CTTGGGA916350.043.5728724
Produced by FastQC (version 0.11.5)