##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527092_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2484715
Sequences flagged as poor quality	0
Sequence length	52
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.395124994214626	33.0	31.0	34.0	31.0	34.0
2	31.87636650481041	31.0	31.0	34.0	30.0	34.0
3	32.629340588357216	34.0	31.0	34.0	31.0	34.0
4	36.2948390459268	37.0	37.0	37.0	35.0	37.0
5	36.31435074042697	37.0	37.0	37.0	35.0	37.0
6	36.51203900648565	37.0	37.0	37.0	35.0	37.0
7	36.49990522051825	37.0	37.0	37.0	35.0	37.0
8	36.62274868546292	37.0	37.0	37.0	35.0	37.0
9	38.72807384347903	39.0	39.0	39.0	39.0	39.0
10	38.18163934294275	39.0	39.0	39.0	37.0	39.0
11	37.96076974622844	39.0	38.0	39.0	35.0	39.0
12	37.48759475432796	39.0	37.0	39.0	35.0	39.0
13	37.41508663971522	39.0	37.0	39.0	35.0	39.0
14	38.24369877430611	40.0	38.0	40.0	35.0	40.0
15	38.11398409877994	40.0	38.0	40.0	35.0	40.0
16	32.98469361677295	40.0	35.0	40.0	3.0	40.0
17	34.6862384619564	40.0	34.0	40.0	17.0	40.0
18	36.735007837921046	40.0	34.0	40.0	31.0	40.0
19	37.60404593685795	40.0	37.0	40.0	33.0	40.0
20	37.86116637119348	40.0	37.0	40.0	34.0	40.0
21	38.07922397538551	40.0	38.0	40.0	34.0	40.0
22	38.118901765393616	40.0	38.0	40.0	34.0	40.0
23	38.09745222289075	40.0	38.0	40.0	34.0	40.0
24	38.10481524037968	40.0	38.0	40.0	34.0	40.0
25	38.062943234938416	40.0	38.0	40.0	34.0	40.0
26	37.9958393618584	40.0	37.0	40.0	34.0	40.0
27	37.891546112934485	40.0	37.0	40.0	34.0	40.0
28	37.777076244156774	40.0	37.0	40.0	34.0	40.0
29	37.60497401110389	40.0	37.0	40.0	34.0	40.0
30	37.581242516747395	40.0	37.0	40.0	33.0	40.0
31	37.557044972964704	40.0	36.0	40.0	33.0	40.0
32	37.49748200497844	40.0	36.0	40.0	33.0	40.0
33	37.15717215052833	40.0	36.0	40.0	33.0	40.0
34	36.98658598672282	39.0	35.0	40.0	31.0	40.0
35	36.96750452265149	39.0	35.0	40.0	32.0	40.0
36	37.01319628206857	39.0	35.0	40.0	32.0	40.0
37	36.748340151687415	39.0	35.0	40.0	32.0	40.0
38	36.798654976526485	39.0	35.0	40.0	32.0	40.0
39	36.43910669835373	39.0	35.0	40.0	31.0	40.0
40	36.42365744159793	39.0	35.0	40.0	31.0	40.0
41	36.404523255182184	38.0	35.0	40.0	31.0	40.0
42	36.34890118182568	38.0	35.0	40.0	31.0	40.0
43	36.31116566688735	38.0	35.0	40.0	31.0	40.0
44	36.54244973769627	39.0	35.0	40.0	32.0	40.0
45	36.609526645913114	39.0	35.0	40.0	32.0	40.0
46	36.52743594335769	39.0	35.0	40.0	32.0	40.0
47	36.23171510615906	38.0	35.0	40.0	31.0	40.0
48	36.1875007797675	38.0	35.0	40.0	31.0	40.0
49	36.09594380039562	38.0	35.0	40.0	31.0	40.0
50	35.618852061504036	37.0	35.0	40.0	30.0	40.0
51	35.679842959856565	37.0	35.0	40.0	30.0	40.0
52	35.37512632233476	37.0	34.0	40.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	4.0
15	17.0
16	36.0
17	98.0
18	243.0
19	415.0
20	761.0
21	1329.0
22	2208.0
23	3514.0
24	5382.0
25	7454.0
26	10649.0
27	14496.0
28	19498.0
29	25753.0
30	34252.0
31	46431.0
32	65463.0
33	103003.0
34	164901.0
35	202544.0
36	261427.0
37	473889.0
38	684401.0
39	356547.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	0.0	100.0	0.0
4	0.0	0.0	0.0	100.0
5	0.0	0.0	100.0	0.0
6	0.0	0.0	100.0	0.0
7	99.76613011955094	0.1635600058759254	0.06012762027033282	0.010182254302807364
8	99.61770263390368	0.23994703617919966	0.10661182469619253	0.03573850522092071
9	97.8670793229807	1.2656179883809613	0.2710169979253154	0.5962856907130194
10	52.49596030128204	35.5409372905947	4.365490609587015	7.597611798536251
11	34.70136414035412	21.547783146155595	24.093185737599683	19.657666975890596
12	30.83717850940651	16.145392932388624	26.73638626562805	26.281042292576817
13	25.470003602022768	16.339016748399715	30.513318428874136	27.67766122070338
14	25.84912152902848	17.73865413135913	30.32150568576275	26.09071865384964
15	27.51579154953385	18.56591198588168	29.294989566207796	24.623306898376676
16	30.266730792062674	27.92646239105893	22.00187949120925	19.80492732566914
17	32.01071350235339	19.162922105754586	24.014907142267823	24.8114572496242
18	29.133441863553767	20.521629241180577	24.587045194318062	25.75788370094759
19	29.18411165868118	21.627470353742783	23.975224522731985	25.213193464844057
20	28.03878915690532	22.865318557661542	24.860597694302967	24.23529459113017
21	30.464982905484128	20.823394232336508	25.482922588707357	23.228700273472008
22	29.519844328222757	20.26795829702803	26.141831155685864	24.070366219063352
23	29.75749733872899	20.967837357604395	24.99147789585526	24.283187407811358
24	31.1171703797015	19.368619741097067	24.289948746636938	25.2242611325645
25	28.79412729427721	19.59633197368712	24.152025483808	27.457515248227665
26	27.124599803196748	20.249485353451	24.78787305586355	27.838041787488706
27	26.795668718545183	18.41498924423928	24.83810014428214	29.951241892933396
28	27.360039280158894	22.01186051518987	23.23139675978935	27.396703444861885
29	29.616515375002766	21.96694590727709	22.532805573275002	25.883733144445138
30	29.394236361111837	20.03098946961724	23.353865533874107	27.220908635396817
31	30.673095304692893	21.634714645341617	22.883308548465315	24.80888150150017
32	33.31508845078812	19.7863336438988	23.17348267306311	23.725095232249977
33	32.7557888932936	19.644587004948253	24.69546809191396	22.904156009844186
34	28.11863734875026	22.43798584545914	26.790678206554873	22.652698599235727
35	26.446654847739076	24.92169122011981	26.69549626415907	21.93615766798204
36	27.699635571886517	26.825652036551478	22.327389660383584	23.147322731178424
37	28.9420718271512	26.36889945124491	21.261512889808287	23.4275158317956
38	29.90604556256955	26.47180863801281	20.806732361659183	22.815413437758455
39	29.229871433947153	23.77427592299318	21.887459930012092	25.10839271304757
40	28.33934676612811	23.544994093890043	21.997532916249952	26.118126223731895
41	25.295979619392966	24.22893571294897	22.040636451263023	28.43444821639504
42	24.694542432431888	22.202465876368116	23.57368953783432	29.529302153365677
43	24.949782973097516	21.420525090402723	24.860396463980777	28.76929547251898
44	25.971952517693175	20.676214374686836	25.16493843358293	28.18689467403706
45	27.112002785027663	22.157269546004272	22.961345667410548	27.769382001557524
46	26.496801444028794	23.53871570783772	23.325733534831965	26.638749313301524
47	26.33968080846294	22.030413950895777	24.88816624844298	26.7417389921983
48	27.686314124557544	21.809020350422482	25.420420450635184	25.084245074384786
49	27.98316909585204	21.847696818347377	24.852427743222062	25.316706342578527
50	25.617143213608003	21.662162461288318	25.370716561054284	27.3499777640494
51	24.10678890737972	23.04690880040568	25.554117876698136	27.29218441551647
52	25.000332030031615	22.372907959262932	25.846666519097766	26.780093491607687
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.0
4	2.0
5	6.0
6	10.0
7	67.0
8	124.0
9	774.5
10	1425.0
11	2299.0
12	3173.0
13	3914.0
14	3986.5
15	3318.0
16	3428.5
17	3539.0
18	4292.0
19	5045.0
20	4621.5
21	4198.0
22	5454.5
23	6711.0
24	9183.0
25	11655.0
26	11656.5
27	11658.0
28	13498.5
29	15339.0
30	19794.5
31	24250.0
32	25856.0
33	27462.0
34	32946.5
35	38431.0
36	38305.0
37	38179.0
38	43389.0
39	51948.0
40	55297.0
41	59597.0
42	63897.0
43	72238.5
44	80580.0
45	87870.0
46	95160.0
47	105758.0
48	116356.0
49	119103.0
50	121850.0
51	126773.0
52	131696.0
53	147325.0
54	162954.0
55	186657.5
56	210361.0
57	229094.0
58	247827.0
59	244688.5
60	241550.0
61	230796.5
62	220043.0
63	197926.5
64	140723.0
65	105636.0
66	88335.0
67	71034.0
68	56885.5
69	42737.0
70	40568.5
71	38400.0
72	35909.5
73	33419.0
74	23923.0
75	14427.0
76	9358.0
77	4289.0
78	3408.0
79	2527.0
80	1729.0
81	931.0
82	524.0
83	117.0
84	68.5
85	20.0
86	13.5
87	7.0
88	8.0
89	8.0
90	7.0
91	4.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2484715.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	7.795108979714295
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.17223408388591	5.31409994074484
2	10.596530830039804	1.6520222525412531
3	4.607610801549698	1.077504850025659
4	2.4188562301103533	0.7542099167988433
5	1.345195021764879	0.5242970896813187
6	0.8479770605106425	0.3966044159386945
7	0.5514074803614938	0.30087969811532594
8	0.4080600007282124	0.2544697740750968
9	0.2958281447769515	0.2075411365022732
>10	4.142124790932705	8.81553964150626
>50	3.243098918318748	18.542498668767525
>100	3.1737278184616606	43.83898421072378
>500	0.1351540393767355	7.057322617091328
>1k	0.05980267229059094	9.074992459966994
>5k	0.0011960534458118188	0.791880172379486
>10k+	0.0011960534458118188	1.3971531551413185
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATCTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	16674	0.6710628784387747	No Hit
GATCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	13297	0.5351519188317372	No Hit
GATCTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	8847	0.35605693208275396	No Hit
GATCTTGGGGAGCCAGTAGACTGGTTCTTCACTTTACCTACCGTATGCTAAG	8140	0.3276029645251065	No Hit
GATCTTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	4916	0.19784965277707908	No Hit
GATCTTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT	4773	0.1920944655624488	No Hit
GATCTTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	4705	0.1893577331806666	No Hit
GATCTTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	4474	0.18006089229549466	No Hit
GATCTTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	4383	0.17639850043163904	No Hit
GATCTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	4115	0.16561255516226206	No Hit
GATCTTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	4097	0.16488812600237854	No Hit
GATCTTGGGACATGAATCATTGTTTTTGGGTCTCTGTACACTTAATCAATTT	3698	0.148829946291627	No Hit
GATCTTGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTC	3690	0.1485079777761232	No Hit
GATCTTGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT	3574	0.14383943430131826	No Hit
GATCTTGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	3542	0.1425515602393031	No Hit
GATCTTGGGGGGGCTGGAGAGATGGCTCAGGGGTTAAGAGCACTGACTGCTC	3505	0.14106245585509808	No Hit
GATCTTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	3461	0.13929162901982722	No Hit
GATCTTGGGATTTCTCTTCTATATCAGGCTCTTGTTAATTAGTCTTTTTTGT	3380	0.13603169780035135	No Hit
GATCTTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT	3014	0.12130163821605294	No Hit
GATCTTGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA	2912	0.11719653964337963	No Hit
GATCTTGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACT	2843	0.11441956119715943	No Hit
GATCTTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	2823	0.11361463990839996	No Hit
GATCTTGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC	2748	0.11059618507555194	No Hit
GATCTTGGGGGGCTGGAGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCT	2668	0.10737649992051401	No Hit
GATCTTGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACG	2631	0.10588739553630899	No Hit
GATCTTGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	2574	0.10359336986334448	No Hit
GATCTTGGGATGTACATACTGACTGTCAATACAGCCTCCTTGCTATGTCCAC	2546	0.10246648005908121	No Hit
GATCTTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	2523	0.10154082057700782	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	1.2073819331392132E-4	0.0	0.0	0.0	0.0
8	2.4147638662784263E-4	0.0	0.0	0.0	0.0
9	5.634449021316328E-4	0.0	0.0	0.0	0.0
10	0.007968720758718807	0.0	0.0	0.0	0.0
11	0.012798248491275659	0.0	0.0	0.0	0.0
12	0.016460640355131274	0.0	0.0	0.0	0.0
13	0.02060598499224257	0.0	0.0	0.0	0.0
14	0.027286831688946217	0.0	0.0	0.0	0.0
15	0.03392743232121189	0.0	0.0	0.0	0.0
16	0.043063288948631935	0.0	0.0	0.0	0.0
17	0.04930142893651787	0.0	0.0	0.0	0.0
18	0.054573663377892434	0.0	0.0	0.0	0.0
19	0.06503764013176562	0.0	0.0	0.0	0.0
20	0.07308685301936037	0.0	0.0	0.0	0.0
21	0.08182024900240067	0.0	0.0	0.0	0.0
22	0.08842060357022838	0.0	0.0	0.0	0.0
23	0.09655030858669908	0.0	0.0	0.0	0.0
24	0.10383484624997233	0.0	0.0	0.0	0.0
25	0.10975101772235447	0.0	0.0	0.0	0.0
26	0.11719653964337963	0.0	0.0	0.0	0.0
27	0.12484329188659464	0.0	0.0	0.0	0.0
28	0.1358304674781615	0.0	0.0	0.0	0.0
29	0.14371869610800433	0.0	0.0	0.0	0.0
30	0.15156667867340923	0.0	0.0	0.0	0.0
31	0.15792555685460907	0.0	0.0	0.0	0.0
32	0.16738338199753292	0.0	0.0	0.0	0.0
33	0.17579480946506942	0.0	0.0	0.0	0.0
34	0.18464894364142367	0.0	0.0	0.0	0.0
35	0.20175352102756253	0.0	0.0	0.0	0.0
36	0.21475299984102805	0.0	0.0	0.0	0.0
37	0.22610239001253665	0.0	0.0	0.0	0.0
38	0.23560046121989847	0.0	0.0	0.0	0.0
39	0.24469607178288053	0.0	0.0	0.0	0.0
40	0.2543148811835563	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAG	20	6.312878E-4	46.0	32
ACGTTAT	20	6.312878E-4	46.0	37
CGCATAA	20	6.312878E-4	46.0	27
CACGCTA	30	1.8622832E-6	46.0	32
GCGCATA	20	6.312878E-4	46.0	15
CGAATAC	25	3.4183104E-5	46.0	29
TTACGAA	20	6.312878E-4	46.0	35
CACGTAA	25	3.4183104E-5	46.0	38
CGGTAAT	40	5.6152203E-9	46.0	27
ATATCGT	55	1.8189894E-12	45.999996	33
GCGTAAC	35	1.0202166E-7	45.999996	34
ACCGTAT	1090	0.0	45.155964	40
TTGGGAT	29675	0.0	44.116596	5
GATCTTG	259535	0.0	43.941357	1
CCGTATG	1115	0.0	43.93722	41
ATCTTGG	258735	0.0	43.915432	2
TCTTGGG	253525	0.0	43.898	3
CTTGGGG	134060	0.0	43.7525	4
TTGGGAC	15665	0.0	43.650814	5
CTTGGGA	91635	0.0	43.572872	4
>>END_MODULE