##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527091_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2412355
Sequences flagged as poor quality	0
Sequence length	52
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.400706363698546	33.0	31.0	34.0	31.0	34.0
2	31.92483071521397	31.0	31.0	34.0	30.0	34.0
3	32.624624899734904	34.0	31.0	34.0	31.0	34.0
4	36.284441137394786	37.0	37.0	37.0	35.0	37.0
5	36.31844027931213	37.0	37.0	37.0	35.0	37.0
6	36.50272907594446	37.0	37.0	37.0	35.0	37.0
7	36.49598587272603	37.0	37.0	37.0	35.0	37.0
8	36.644554802257545	37.0	37.0	37.0	35.0	37.0
9	38.67493756101403	39.0	39.0	39.0	39.0	39.0
10	38.17462811236323	39.0	39.0	39.0	37.0	39.0
11	37.887194048968745	39.0	38.0	39.0	35.0	39.0
12	37.44914450816733	39.0	37.0	39.0	35.0	39.0
13	37.32204712822118	39.0	37.0	39.0	35.0	39.0
14	38.171822969670714	40.0	38.0	40.0	35.0	40.0
15	38.226123435398186	40.0	38.0	40.0	35.0	40.0
16	38.25385815935051	40.0	38.0	40.0	35.0	40.0
17	38.20310194809636	40.0	38.0	40.0	34.0	40.0
18	38.166532703520005	40.0	38.0	40.0	34.0	40.0
19	38.12862451836484	40.0	38.0	40.0	34.0	40.0
20	38.09781023108125	40.0	38.0	40.0	34.0	40.0
21	33.8616119932597	40.0	34.0	40.0	10.0	40.0
22	34.64364117221553	40.0	34.0	40.0	17.0	40.0
23	36.71473767335239	40.0	34.0	40.0	30.0	40.0
24	37.519157835393216	40.0	36.0	40.0	33.0	40.0
25	37.78588806373855	40.0	37.0	40.0	34.0	40.0
26	37.88861133622539	40.0	37.0	40.0	34.0	40.0
27	37.857606778438495	40.0	37.0	40.0	34.0	40.0
28	37.74570533773014	40.0	37.0	40.0	34.0	40.0
29	37.74395352259514	40.0	37.0	40.0	34.0	40.0
30	37.637636251712536	40.0	37.0	40.0	34.0	40.0
31	37.51753784165266	40.0	36.0	40.0	33.0	40.0
32	37.49147907335363	40.0	36.0	40.0	33.0	40.0
33	37.17069585529493	40.0	36.0	40.0	33.0	40.0
34	36.98727799183785	39.0	35.0	40.0	32.0	40.0
35	36.96500349243789	39.0	35.0	40.0	32.0	40.0
36	36.9460241133664	39.0	35.0	40.0	32.0	40.0
37	36.89816672919201	39.0	35.0	40.0	32.0	40.0
38	36.87840388334221	39.0	35.0	40.0	32.0	40.0
39	36.73847215687575	39.0	35.0	40.0	32.0	40.0
40	31.798880347212577	38.0	31.0	40.0	9.0	40.0
41	32.75232832646936	37.0	31.0	40.0	15.0	40.0
42	34.6104702666067	37.0	32.0	40.0	25.0	40.0
43	35.552298894648594	37.0	34.0	40.0	30.0	40.0
44	36.237028961326175	38.0	35.0	40.0	31.0	40.0
45	36.48583189455947	39.0	35.0	40.0	32.0	40.0
46	36.49715236770707	39.0	35.0	40.0	32.0	40.0
47	36.450460234915674	38.0	35.0	40.0	32.0	40.0
48	36.30918873880503	38.0	35.0	40.0	32.0	40.0
49	36.1532444437075	38.0	35.0	40.0	31.0	40.0
50	36.07212495673315	37.0	35.0	40.0	31.0	40.0
51	35.87068072485186	37.0	35.0	40.0	31.0	40.0
52	35.50808939811927	37.0	35.0	40.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	2.0
15	20.0
16	48.0
17	83.0
18	259.0
19	476.0
20	872.0
21	1418.0
22	2317.0
23	3830.0
24	5571.0
25	8024.0
26	11040.0
27	15364.0
28	20847.0
29	27722.0
30	37460.0
31	52604.0
32	79967.0
33	92631.0
34	182500.0
35	241363.0
36	302585.0
37	306884.0
38	650050.0
39	368417.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	0.0	100.0	0.0
4	0.0	0.0	0.0	100.0
5	0.0	0.0	100.0	0.0
6	0.0	0.0	100.0	0.0
7	99.77586217617225	0.15756387430539867	0.05720551079754017	0.009368438724814548
8	99.64039289408068	0.2340451550455882	0.09115573785781943	0.03440621301591184
9	97.79849980620597	1.2712473910349016	0.3167029728211644	0.6135498299379651
10	52.448582401843844	35.5938491639912	4.392098177921574	7.56547025624338
11	34.740409268121816	21.535760698570485	24.05997458914629	19.66385544416141
12	30.868259439427447	16.149944763519468	26.720072294500604	26.261723502552485
13	25.57629370469935	16.35074439707257	30.46060799509193	27.61235390313615
14	25.90149459760276	17.71045306350019	30.305199690758617	26.082852648138438
15	27.485921433619843	18.446580208965926	29.4384118423698	24.629086515044428
16	32.09067487994097	19.891309529484673	24.98691113040991	23.031104460164446
17	31.93439605696508	19.257240331543244	24.037548370782908	24.770815240708767
18	29.205444472310255	20.497563584132518	24.616526174630184	25.680465768927046
19	29.227000172031065	21.609381703770797	23.96260086098439	25.20101726321375
20	28.02083441284554	22.851777619794763	24.875318931086014	24.25206903627368
21	34.84288174833306	20.049992642044806	24.106443703352117	21.000681906270014
22	29.58142561936365	20.233796435433423	26.136161551678754	24.04861639352417
23	29.805521989922713	20.946502484087127	25.027079347774272	24.22089617821589
24	31.218912639308893	19.38458477297081	24.251032704556337	25.145469883163962
25	28.851475010933296	19.65369939333141	24.09272267141445	27.402102924320843
26	27.174690292266273	20.233050276596938	24.751954003453058	27.840305427683738
27	26.8079532241316	18.39903330977406	24.898822934435437	29.8941905316589
28	27.357665020281015	22.035189679794225	23.241894331472775	27.36525096845199
29	29.434432328575188	21.951454077032608	22.553106818855433	26.061006775536764
30	29.39467864389777	20.018778330718323	23.349921549688997	27.23662147569491
31	30.715960130246174	21.566270304329173	22.9266007697872	24.791168795637457
32	33.282331995083645	19.79219476403763	23.166988274942952	23.75848496593578
33	32.729635563588275	19.706676670722178	24.637957514544915	22.92573025114463
34	28.145691658151474	22.51484545185099	26.66580996578033	22.67365292421721
35	26.503686231918604	24.924067975070006	26.681313488271837	21.89093230473956
36	27.76780366073816	26.84070130639976	22.291412333591033	23.100082699271045
37	28.800487490439842	26.35714892708577	21.282149600701388	23.560213981773
38	29.931001034259054	26.49862893313795	20.816753753075314	22.75361627952768
39	28.9684561351874	23.821784107231313	21.896113963326293	25.31364579425499
40	28.702740682859694	27.950612575678125	20.266876143851135	23.07977059761105
41	25.283882347332792	24.20771403877124	22.01073225126484	28.49767136263112
42	24.609520572220923	22.255389443096064	23.604651885812828	29.530438098870192
43	24.936918488365105	21.42752621401079	24.913704657896538	28.72185063972757
44	25.971509168426703	20.659645864725547	25.1790055775373	28.189839389310446
45	27.119060005679096	22.161207616623592	22.956778749396335	27.762953628300973
46	26.528599646403617	23.506490545545745	23.327619691131694	26.637290116918944
47	26.17732464749177	21.939639895454857	24.921207699530125	26.96182775752325
48	27.62794033216504	21.838825545991366	25.4359743901706	25.097259731672995
49	27.944767664792288	21.92542142429286	24.81906684546843	25.31074406544642
50	25.603901581649467	21.599847451971208	25.490858517921282	27.305392448458043
51	24.234948836303115	23.023477058724772	25.542467837445155	27.19910626752696
52	24.92850347482025	22.510202685757278	25.78870025348674	26.772593585935734
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	1.0
5	3.5
6	6.0
7	54.5
8	103.0
9	672.5
10	1242.0
11	2091.5
12	2941.0
13	3648.0
14	3741.5
15	3128.0
16	3324.5
17	3521.0
18	4012.5
19	4504.0
20	4311.5
21	4119.0
22	5310.0
23	6501.0
24	8617.0
25	10733.0
26	11077.5
27	11422.0
28	12898.5
29	14375.0
30	18756.5
31	23138.0
32	24859.0
33	26580.0
34	31616.5
35	36653.0
36	37061.0
37	37469.0
38	41979.5
39	50160.0
40	53830.0
41	57637.5
42	61445.0
43	69483.0
44	77521.0
45	84389.0
46	91257.0
47	102335.5
48	113414.0
49	116567.0
50	119720.0
51	124059.5
52	128399.0
53	142215.0
54	156031.0
55	179152.0
56	202273.0
57	220900.5
58	239528.0
59	236050.5
60	232573.0
61	224854.0
62	217135.0
63	194463.0
64	138350.0
65	104909.0
66	87681.5
67	70454.0
68	56494.5
69	42535.0
70	39827.0
71	37119.0
72	34845.5
73	32572.0
74	23572.0
75	14572.0
76	9536.0
77	4500.0
78	3478.0
79	2456.0
80	1681.0
81	906.0
82	502.5
83	99.0
84	60.5
85	22.0
86	14.0
87	6.0
88	4.0
89	2.0
90	2.0
91	2.5
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2412355.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.055568094089775
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.71837525683414	5.535655511966414
2	10.440316917394162	1.6820536770389225
3	4.5181101640418735	1.0918783224911532
4	2.3421073939288615	0.7546802238186036
5	1.3127246000527864	0.5287371202255993
6	0.796127314810525	0.3847954677612619
7	0.520466537564049	0.2934857543829632
8	0.38183677244141917	0.24607296969834513
9	0.2736606419646946	0.19840427424170273
>10	4.180606827695203	9.262805480717667
>50	3.30220528359117	19.397336963643625
>100	3.0269208943192174	42.8438828508931
>500	0.1291904809302578	6.95004616771003
>1k	0.05433244525104299	8.38913377420677
>5k	0.0018110815083680998	1.047267896928471
>10k+	0.0012073876722454	1.3937635442753646
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATCTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	15684	0.6501530661946521	No Hit
GATCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	12976	0.537897614571653	No Hit
GATCTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	8569	0.3552130594377693	No Hit
GATCTTGGGGAGCCAGTAGACTGGTTCTTCACTTTACCTACCGTATGCTAAG	7847	0.32528379944079544	No Hit
GATCTTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	5119	0.21219928244391892	No Hit
GATCTTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	4651	0.1927991526951879	No Hit
GATCTTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT	4646	0.19259188635171856	No Hit
GATCTTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	4594	0.19043631637963732	No Hit
GATCTTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	4477	0.18558628394245458	No Hit
GATCTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	4153	0.17215542488564078	No Hit
GATCTTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	3900	0.16166774790609176	No Hit
GATCTTGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTC	3635	0.1506826317022163	No Hit
GATCTTGGGGGGGCTGGAGAGATGGCTCAGGGGTTAAGAGCACTGACTGCTC	3445	0.14280651065038105	No Hit
GATCTTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	3426	0.14201889854519753	No Hit
GATCTTGGGATTTCTCTTCTATATCAGGCTCTTGTTAATTAGTCTTTTTTGT	3370	0.13969751549834084	No Hit
GATCTTGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT	3357	0.13915862300532053	No Hit
GATCTTGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	3335	0.1382466510940554	No Hit
GATCTTGGGACATGAATCATTGTTTTTGGGTCTCTGTACACTTAATCAATTT	3329	0.13799793148189218	No Hit
GATCTTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT	2876	0.11921960076356922	No Hit
GATCTTGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACG	2685	0.11130202644304009	No Hit
GATCTTGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACT	2676	0.11092894702479526	No Hit
GATCTTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	2654	0.11001697511353013	No Hit
GATCTTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	2529	0.10483531652679644	No Hit
GATCTTGGGGGGCTGGAGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCT	2528	0.10479386325810255	No Hit
GATCTTGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC	2514	0.1042135174963884	No Hit
GATCTTGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA	2506	0.10388189134683742	No Hit
GATCTTGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	2477	0.1026797465547152	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	2.0726634346934843E-4	0.0	0.0	0.0	0.0
8	3.7307941824482713E-4	0.0	0.0	0.0	0.0
9	6.217990304080453E-4	0.0	0.0	0.0	0.0
10	0.00775176124575363	0.0	0.0	0.0	0.0
11	0.01297487310118121	0.0	0.0	0.0	0.0
12	0.017700545732282354	0.0	0.0	0.0	0.0
13	0.021472793183424495	0.0	0.0	0.0	0.0
14	0.02769078348750495	0.0	0.0	0.0	0.0
15	0.0343233064785241	0.0	0.0	0.0	0.0
16	0.040831469663461635	0.0	0.0	0.0	0.0
17	0.047629805729256264	0.0	0.0	0.0	0.0
18	0.05351616988378576	0.0	0.0	0.0	0.0
19	0.06217990304080453	0.0	0.0	0.0	0.0
20	0.07047055677957846	0.0	0.0	0.0	0.0
21	0.0772688928453731	0.0	0.0	0.0	0.0
22	0.08448176159810641	0.0	0.0	0.0	0.0
23	0.09210916303777844	0.0	0.0	0.0	0.0
24	0.0994049383278995	0.0	0.0	0.0	0.0
25	0.10583019497544931	0.0	0.0	0.0	0.0
26	0.11387212910206002	0.0	0.0	0.0	0.0
27	0.12158243707911978	0.0	0.0	0.0	0.0
28	0.1327748196264646	0.0	0.0	0.0	0.0
29	0.14048512760352436	0.0	0.0	0.0	0.0
30	0.148236888849278	0.0	0.0	0.0	0.0
31	0.15474505203421554	0.0	0.0	0.0	0.0
32	0.16262117308605076	0.0	0.0	0.0	0.0
33	0.17107763989960018	0.0	0.0	0.0	0.0
34	0.17969991978792507	0.0	0.0	0.0	0.0
35	0.19528634881682008	0.0	0.0	0.0	0.0
36	0.20801250230583807	0.0	0.0	0.0	0.0
37	0.2187488988975503	0.0	0.0	0.0	0.0
38	0.228075884353671	0.0	0.0	0.0	0.0
39	0.2365323511672204	0.0	0.0	0.0	0.0
40	0.24805635986411617	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGATG	20	6.31284E-4	46.0	20
CGTATAA	25	3.4182813E-5	46.0	26
GCGCATA	40	5.6152203E-9	46.0	15
CCGCGTA	30	1.8622595E-6	46.0	45
CCGGTAA	20	6.31284E-4	46.0	30
ACCGTAT	920	0.0	45.500004	40
TTGGGAT	29375	0.0	44.28528	5
GATCTTG	251355	0.0	44.05371	1
ATCTTGG	250565	0.0	44.035645	2
TCTTGGG	245635	0.0	43.97936	3
CTTGGGG	130205	0.0	43.800774	4
CTTGGGA	88940	0.0	43.755337	4
TTGGGAC	14785	0.0	43.619884	5
TTGGGGC	29005	0.0	43.5418	5
TGGGATC	8935	0.0	43.477337	6
TTGGGGG	49195	0.0	43.24626	5
TTGGGAG	31260	0.0	43.204094	5
TACCGTA	980	0.0	42.94898	39
TTGGGGT	21015	0.0	42.93552	5
TGGGATT	10255	0.0	42.83764	6
>>END_MODULE