FastQCFastQC Report
Fri 17 Jun 2016
SRR1527090_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527090_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7933529
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC1032521.3014636991936375No Hit
TAGTCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC505990.63778679072075No Hit
TAGTCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG477130.6014095366639487No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAA385750.486227503548547No Hit
TAGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT316560.3990153688226261No Hit
TAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA265340.334453935947042No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAA229530.2893163937511289No Hit
TAGTCGGGGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAA206160.2598591370876693No Hit
TAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA200280.2524475551800466No Hit
TAGTCGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT192780.24299400682848707No Hit
TAGTCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTAGTCGGGGA180660.22771707269236677No Hit
TAGTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT132200.1666345456101566No Hit
TAGTCGGGGGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAA126240.1591221258534506No Hit
TAGTCGGGGAGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAA117720.14838289492607892No Hit
TAGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC108000.13613109626245773No Hit
TAGTCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC104660.13192111606322987No Hit
TAGTCGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA99000.12478683824058624No Hit
TAGTCGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT96730.12192556427284755No Hit
TAGTCGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGAC95150.11993401675345235No Hit
TAGTCGGGGGGGGCTGGAGAGATGGCTCAGGGGTTAAGAGCACTGACTGC91190.1149425432238289No Hit
TAGTCGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT88820.11195522194473606No Hit
TAGTCGGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGA85960.10835026884000803No Hit
TAGTCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC83680.10547639014113393No Hit
TAGTCGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC82400.10386298455580109No Hit
TAGTCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG81020.10212353165911413No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGGG1628650.041.401045
CGGGGAT506050.041.130725
TCGGGGG4020500.041.073054
CGGGGGC783250.040.7249265
CGGGGGT620100.039.9909675
GTCGGGG7833800.039.9748043
TCGGGGC860100.039.8562934
CGGGGGA1035500.039.6042485
AGTCGGG8623350.039.5417562
CGGGGAC352050.039.5193865
TAGTCGG8790600.039.45891
CGGGGAG776650.039.4223945
TCGGGGT655350.038.3367654
CGGGGCT316800.038.0833325
GTCGGGT225950.037.836693
CGGGGCA366850.037.7691155
TCGGGGA2347900.037.7426644
CGGGGTC124550.037.5351265
CGGGGTA166750.037.495655
TCGGGAT62650.037.3982474
Produced by FastQC (version 0.11.5)