##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527090_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7933529
Sequences flagged as poor quality	0
Sequence length	50
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.275256824548066	33.0	31.0	34.0	31.0	34.0
2	32.662471139892475	34.0	31.0	34.0	31.0	34.0
3	32.958928996162996	34.0	31.0	34.0	31.0	34.0
4	35.87165131683517	37.0	35.0	37.0	35.0	37.0
5	36.267953517280894	37.0	35.0	37.0	35.0	37.0
6	36.27224303333359	37.0	37.0	37.0	35.0	37.0
7	36.59055371197358	37.0	37.0	37.0	35.0	37.0
8	36.63060600144021	37.0	37.0	37.0	35.0	37.0
9	38.51194594486262	39.0	39.0	39.0	37.0	39.0
10	37.935779903243564	39.0	38.0	39.0	35.0	39.0
11	36.662926422781084	38.0	35.0	39.0	33.0	39.0
12	36.52480113200569	39.0	35.0	39.0	33.0	39.0
13	36.26531673357468	38.0	35.0	39.0	33.0	39.0
14	36.98859057551816	39.0	35.0	40.0	33.0	40.0
15	37.10288662208205	39.0	35.0	40.0	33.0	40.0
16	36.969171222541696	39.0	35.0	40.0	33.0	40.0
17	36.88982355771309	39.0	35.0	40.0	32.0	40.0
18	36.817106863792894	39.0	35.0	40.0	32.0	40.0
19	36.73220366371636	39.0	35.0	40.0	31.0	40.0
20	36.47097351002309	39.0	35.0	40.0	31.0	40.0
21	36.70964825363341	39.0	35.0	40.0	31.0	40.0
22	36.59847830643841	39.0	35.0	40.0	31.0	40.0
23	36.59908648471569	38.0	35.0	40.0	31.0	40.0
24	36.5983860398065	38.0	35.0	40.0	32.0	40.0
25	36.48366407937754	38.0	35.0	40.0	32.0	40.0
26	36.361877923431045	38.0	35.0	40.0	31.0	40.0
27	36.22236296104798	38.0	35.0	40.0	31.0	40.0
28	35.99074434592727	38.0	34.0	40.0	31.0	40.0
29	36.27907958740681	38.0	35.0	40.0	31.0	40.0
30	36.26527236492108	38.0	35.0	40.0	31.0	40.0
31	36.13347187613482	38.0	35.0	40.0	31.0	40.0
32	35.42233172652423	37.0	35.0	40.0	29.0	40.0
33	35.56069209553529	37.0	35.0	40.0	30.0	40.0
34	35.225483640382485	37.0	34.0	40.0	28.0	40.0
35	35.252434572306974	37.0	34.0	40.0	29.0	40.0
36	35.077882742976044	36.0	34.0	40.0	29.0	40.0
37	34.92816828425282	36.0	34.0	40.0	29.0	40.0
38	34.706624378634025	35.0	34.0	40.0	28.0	40.0
39	34.57379799078065	35.0	34.0	40.0	27.0	40.0
40	34.445782198565105	35.0	34.0	40.0	27.0	40.0
41	34.27664107612136	35.0	34.0	39.0	26.0	40.0
42	34.1483631054982	35.0	34.0	39.0	26.0	40.0
43	34.091424383776754	35.0	33.0	39.0	26.0	40.0
44	33.91167663217718	35.0	33.0	39.0	26.0	40.0
45	33.78126934432331	35.0	33.0	39.0	25.0	40.0
46	33.4951936269471	35.0	33.0	38.0	23.0	40.0
47	33.367165103953106	35.0	33.0	38.0	23.0	40.0
48	33.246924666185755	35.0	33.0	37.0	23.0	40.0
49	33.0221840747037	35.0	33.0	37.0	23.0	40.0
50	32.555364327778975	35.0	32.0	37.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	5.0
11	8.0
12	19.0
13	32.0
14	98.0
15	234.0
16	595.0
17	1448.0
18	3140.0
19	5861.0
20	9375.0
21	14050.0
22	20920.0
23	29878.0
24	41044.0
25	56061.0
26	75520.0
27	100095.0
28	132532.0
29	163687.0
30	192238.0
31	231424.0
32	305244.0
33	465794.0
34	935887.0
35	769475.0
36	1037081.0
37	1264681.0
38	1603132.0
39	473971.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	100.0	0.0	0.0
3	100.0	0.0	0.0	0.0
4	0.0	0.0	100.0	0.0
5	0.0	0.0	0.0	100.0
6	100.0	0.0	0.0	0.0
7	99.34053307172634	0.4121873128591324	0.1719663468804362	0.07531326853409119
8	98.26950906715032	1.2239193932485783	0.23609921889741628	0.2704723207036869
9	91.32739037066607	4.254600947447221	2.6052844831095974	1.812724198777114
10	51.435924668580654	28.75234967944278	8.65299666768723	11.158728984289338
11	35.43188661691411	27.305692082300325	19.144431185667816	18.11799011511775
12	35.99332655114767	17.244293176466613	24.830160701498666	21.932219570887053
13	25.01052179931529	17.130459849582703	28.771130728834542	29.087887622267466
14	23.473034509611043	20.864636657911	30.257896580449884	25.40443225202807
15	26.38121068190461	20.188922231203794	27.965915294442105	25.463951792449492
16	31.733015660496104	22.550834565550844	24.927053269736582	20.789096504216474
17	33.13365338426317	19.76527721774257	24.70132774456361	22.39974165343065
18	30.658474935933306	20.706611143666333	25.0376849949121	23.597228925488267
19	30.036973457839505	23.00282761933561	23.411561235863637	23.54863768696125
20	27.880795545084663	24.043285150908254	24.406490478575172	23.66942882543191
21	27.2122153962001	23.896616499416588	27.981141809653685	20.910026294729622
22	27.239882781042336	22.210532034356966	28.171536273454095	22.378048911146603
23	28.16039369113039	23.808698499747084	26.37701330643652	21.653894502686004
24	31.569406250358444	21.720636554048014	23.301849656061005	23.408107539532534
25	28.43153406258426	20.284781211488607	23.178058591580115	28.105626134347023
26	27.23340394923873	20.3095873223631	23.686760330743102	28.770248397655067
27	27.96091121618135	19.745626441902463	23.93304417239793	28.360418169518255
28	28.351519229336652	24.721041544059396	20.620230921195347	26.3072083054086
29	31.70587767436156	24.395158825284437	20.056496925895146	23.842466574458857
30	29.840919469759296	23.875642226807265	23.695898760816277	22.587539542617165
31	30.91759039388398	23.873007837999964	22.35433941188089	22.855062356235166
32	32.56506656747584	24.200907313756588	21.559006086698616	21.675020032068957
33	32.8210812615672	24.378583603841367	21.68420888106667	21.116126253524754
34	26.804780066979024	25.693269665996056	26.872782591454573	20.629167675570354
35	25.406171704924756	30.50007127975457	24.740137711729545	19.353619303591127
36	26.08441968259018	31.920095080007897	21.437698154251407	20.55778708315051
37	28.13072215403763	29.88953591774858	20.825574596122358	21.15416733209143
38	29.3384066535838	29.254938124005093	20.195791809672595	21.21086341273852
39	28.849544761227946	27.61512562694357	20.670939754553114	22.86438985727537
40	27.512018926255895	26.29512036824974	21.260740333841348	24.932120371653017
41	23.51055879420117	28.428269437220184	22.202868357826635	25.858303410752015
42	23.084934837951685	27.447331446068958	23.00117639955687	26.466557316422488
43	23.842365736609775	24.88641561655601	23.114555956119904	28.156662690714306
44	24.21401623413742	24.763822001532986	22.95484140790309	28.0673203564265
45	26.955545256089692	25.284762934628464	21.779223344365413	25.980468464916434
46	26.043000536079212	28.68742270936427	21.419232223138028	23.85034453141849
47	24.571536828062264	26.742096739042616	23.300979929612662	25.385386503282458
48	25.046621749287105	26.16086737692646	25.39526861249262	23.39724226129381
49	27.783171902440895	26.05343725345934	23.015066813268092	23.148324030831677
50	24.073070130581232	26.760877788434378	22.918779272124677	26.247272808859712
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	122.5
6	245.0
7	739.0
8	1233.0
9	11893.5
10	22554.0
11	28150.5
12	33747.0
13	25390.0
14	17033.0
15	19237.5
16	21442.0
17	19815.5
18	18189.0
19	19731.0
20	21273.0
21	23231.5
22	25190.0
23	27194.0
24	29198.0
25	33082.5
26	36967.0
27	46942.5
28	56918.0
29	64492.5
30	72067.0
31	84024.0
32	95981.0
33	122207.0
34	148433.0
35	182999.0
36	217565.0
37	212819.0
38	208073.0
39	201577.0
40	195081.0
41	199830.5
42	204580.0
43	216599.5
44	228619.0
45	239744.0
46	250869.0
47	266826.5
48	282784.0
49	297223.0
50	311662.0
51	333632.5
52	355603.0
53	383739.5
54	411876.0
55	465935.5
56	519995.0
57	577714.0
58	635433.0
59	676903.5
60	718374.0
61	730283.0
62	742192.0
63	725356.0
64	708520.0
65	578940.5
66	449361.0
67	367119.5
68	284878.0
69	244272.5
70	203667.0
71	182894.0
72	162121.0
73	130267.5
74	98414.0
75	88877.0
76	79340.0
77	59288.0
78	39236.0
79	26752.5
80	14269.0
81	10690.5
82	7112.0
83	4761.5
84	2411.0
85	1539.0
86	667.0
87	459.0
88	251.0
89	160.0
90	69.0
91	43.0
92	17.0
93	18.5
94	20.0
95	10.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	7933529.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.033530222664032
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.0810912089194	5.388979735961365
2	10.607523496331792	1.7043172119080057
3	4.812230812945507	1.1597760502269847
4	2.657485594126129	0.8539596334682615
5	1.5383715259413426	0.617927707366778
6	0.9762006676848998	0.47054025403388694
7	0.6814970202815969	0.38323788363613837
8	0.5375742642429	0.3454895278977374
9	0.40619172991333446	0.29368381846094627
>10	5.004653114425911	10.355486174843914
>50	2.9338394176335645	17.058912982709113
>100	2.567022336188059	36.7100094643395
>500	0.12691317098377397	6.789186299493193
>1k	0.062299824818854974	9.135198343324072
>5k	0.004461791167398101	2.651083750385886
>10k+	0.0026440243954951714	6.082211161944318
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TAGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	103252	1.3014636991936375	No Hit
TAGTCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	50599	0.63778679072075	No Hit
TAGTCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	47713	0.6014095366639487	No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAA	38575	0.486227503548547	No Hit
TAGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT	31656	0.3990153688226261	No Hit
TAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	26534	0.334453935947042	No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAA	22953	0.2893163937511289	No Hit
TAGTCGGGGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAA	20616	0.2598591370876693	No Hit
TAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	20028	0.2524475551800466	No Hit
TAGTCGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	19278	0.24299400682848707	No Hit
TAGTCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTAGTCGGGGA	18066	0.22771707269236677	No Hit
TAGTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	13220	0.1666345456101566	No Hit
TAGTCGGGGGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAA	12624	0.1591221258534506	No Hit
TAGTCGGGGAGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAA	11772	0.14838289492607892	No Hit
TAGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC	10800	0.13613109626245773	No Hit
TAGTCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	10466	0.13192111606322987	No Hit
TAGTCGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	9900	0.12478683824058624	No Hit
TAGTCGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT	9673	0.12192556427284755	No Hit
TAGTCGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGAC	9515	0.11993401675345235	No Hit
TAGTCGGGGGGGGCTGGAGAGATGGCTCAGGGGTTAAGAGCACTGACTGC	9119	0.1149425432238289	No Hit
TAGTCGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	8882	0.11195522194473606	No Hit
TAGTCGGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGA	8596	0.10835026884000803	No Hit
TAGTCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	8368	0.10547639014113393	No Hit
TAGTCGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	8240	0.10386298455580109	No Hit
TAGTCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG	8102	0.10212353165911413	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.002697412462978329	0.0	0.0	0.0	0.0
8	0.003806628802894651	0.0	0.0	0.0	0.0
9	0.007663676530330954	0.0	0.0	0.0	0.0
10	0.025587604204887888	0.0	0.0	0.0	0.0
11	0.05086009013139046	0.0	0.0	0.0	0.0
12	0.06119596966242891	0.0	0.0	0.0	0.0
13	0.07091421736783214	0.0	0.0	0.0	0.0
14	0.1010143153191978	0.0	0.0	0.0	0.0
15	0.11973234105528574	0.0	0.0	0.0	0.0
16	0.14220657666972666	0.0	0.0	0.0	0.0
17	0.15323571641321285	0.0	0.0	0.0	0.0
18	0.16499593056255293	0.0	0.0	0.0	0.0
19	0.17993253695801703	0.0	0.0	0.0	0.0
20	0.19316750465020044	0.0	0.0	0.0	0.0
21	0.2054949317006341	0.0	0.0	0.0	0.0
22	0.218225710147401	0.0	0.0	0.0	0.0
23	0.23370431998168784	0.0	0.0	0.0	0.0
24	0.2482879939053604	0.0	0.0	0.0	0.0
25	0.25968267085177354	0.0	0.0	0.0	0.0
26	0.27223698306264466	0.0	0.0	0.0	0.0
27	0.2873248462317337	0.0	0.0	0.0	0.0
28	0.3250886207134303	0.0	0.0	0.0	0.0
29	0.3386261019528636	0.0	0.0	0.0	0.0
30	0.35862981026476365	0.0	0.0	0.0	0.0
31	0.37437311945289414	0.0	0.0	0.0	0.0
32	0.39146513487251383	0.0	0.0	0.0	0.0
33	0.4064269507302488	0.0	0.0	0.0	0.0
34	0.42556093259380534	0.0	0.0	0.0	0.0
35	0.4799629521742468	0.0	0.0	0.0	0.0
36	0.5012271335996881	0.0	0.0	0.0	0.0
37	0.521256051373859	0.0	0.0	0.0	0.0
38	0.5399488676476761	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGGGG	162865	0.0	41.40104	5
CGGGGAT	50605	0.0	41.13072	5
TCGGGGG	402050	0.0	41.07305	4
CGGGGGC	78325	0.0	40.724926	5
CGGGGGT	62010	0.0	39.990967	5
GTCGGGG	783380	0.0	39.974804	3
TCGGGGC	86010	0.0	39.856293	4
CGGGGGA	103550	0.0	39.604248	5
AGTCGGG	862335	0.0	39.541756	2
CGGGGAC	35205	0.0	39.519386	5
TAGTCGG	879060	0.0	39.4589	1
CGGGGAG	77665	0.0	39.422394	5
TCGGGGT	65535	0.0	38.336765	4
CGGGGCT	31680	0.0	38.083332	5
GTCGGGT	22595	0.0	37.83669	3
CGGGGCA	36685	0.0	37.769115	5
TCGGGGA	234790	0.0	37.742664	4
CGGGGTC	12455	0.0	37.535126	5
CGGGGTA	16675	0.0	37.49565	5
TCGGGAT	6265	0.0	37.398247	4
>>END_MODULE