FastQCFastQC Report
Fri 17 Jun 2016
SRR1527089_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527089_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8498638
Sequences flagged as poor quality0
Sequence length52
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC990061.164963138799417No Hit
TAGTCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA540270.6357136284661142No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAA385580.4536962275602279No Hit
TAGTCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT350380.412277826164616No Hit
TAGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC309550.3642348338639674No Hit
TAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA260550.3065785364666668No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAA222360.261641924270689No Hit
TAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA193270.2274129101627814No Hit
TAGTCGGGGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAA188570.22188261224916275No Hit
TAGTCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTAGTCGGGGAAG181270.21329300059609552No Hit
TAGTCGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC170790.20096161290785652No Hit
TAGTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT122870.14457610737155766No Hit
TAGTCGGGGGGGCTGATGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC117470.138222148066549No Hit
TAGTCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT114410.13462157112704412No Hit
TAGTCGGGGGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAA109870.12927953867431463No Hit
TAGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC105020.12357274189111243No Hit
TAGTCGGGGAGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAA104320.12274908049972241No Hit
TAGTCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC96730.11381823769879362No Hit
TAGTCGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC94930.11170025126379074No Hit
TAGTCGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT94570.11127665397679017No Hit
TAGTCGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG85910.10108678590616521No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGGG1759650.043.2852025
TCGGGGG4326700.042.755224
CGGGGGC821850.042.5101935
CGGGGAT541450.042.4572875
TCGGGGC929950.041.728694
CGGGGGT663300.041.6690755
GTCGGGG8436050.041.5475233
AGTCGGG9283550.041.1213042
CGGGGAC371300.041.0382425
TAGTCGG9466950.041.015391
CGGGGAG820900.040.87555
CGGGGGA1133150.040.8119855
TCGGGGT720500.039.9156154
CGGGGCA400050.039.5378075
CGGGGCT348250.039.4021535
TCGGGAT70250.039.2882584
GTCGGGT244450.039.1973843
TCGGGGA2517800.038.900314
CGGGGTC143950.038.4744725
CGGGGTG330900.038.312485
Produced by FastQC (version 0.11.5)