##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527089_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8498638
Sequences flagged as poor quality	0
Sequence length	52
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.273628433167765	33.0	31.0	34.0	31.0	34.0
2	32.581831465230074	34.0	31.0	34.0	31.0	34.0
3	32.91270813040866	34.0	31.0	34.0	31.0	34.0
4	35.80099717154678	37.0	35.0	37.0	35.0	37.0
5	36.2095564018611	37.0	35.0	37.0	35.0	37.0
6	36.287959317716556	37.0	37.0	37.0	35.0	37.0
7	36.61782335004738	37.0	37.0	37.0	35.0	37.0
8	36.62145663811072	37.0	37.0	37.0	35.0	37.0
9	38.515097124974616	39.0	39.0	39.0	37.0	39.0
10	37.90703216209468	39.0	38.0	39.0	35.0	39.0
11	36.738393610835054	39.0	35.0	39.0	33.0	39.0
12	36.644819322813845	39.0	35.0	39.0	33.0	39.0
13	36.494304969808105	39.0	35.0	39.0	33.0	39.0
14	37.212325080795296	40.0	35.0	40.0	33.0	40.0
15	37.213704948957705	40.0	35.0	40.0	33.0	40.0
16	32.238329012248784	38.0	33.0	40.0	3.0	40.0
17	33.6405965285261	38.0	33.0	40.0	16.0	40.0
18	35.561452435084306	38.0	33.0	40.0	27.0	40.0
19	36.52812615386136	38.0	35.0	40.0	31.0	40.0
20	36.87669647771796	39.0	35.0	40.0	31.0	40.0
21	37.126351069430186	39.0	35.0	40.0	33.0	40.0
22	37.19965869825259	39.0	35.0	40.0	33.0	40.0
23	37.11100343372667	39.0	35.0	40.0	33.0	40.0
24	37.051002760677655	39.0	35.0	40.0	33.0	40.0
25	36.96751102941436	39.0	35.0	40.0	33.0	40.0
26	36.86492870975326	39.0	35.0	40.0	33.0	40.0
27	36.7212953416771	39.0	35.0	40.0	32.0	40.0
28	36.55801670808899	38.0	35.0	40.0	32.0	40.0
29	36.28874956198864	38.0	35.0	40.0	31.0	40.0
30	36.11692508846712	38.0	35.0	40.0	31.0	40.0
31	35.93277652254397	38.0	35.0	40.0	31.0	40.0
32	35.83578074510292	37.0	35.0	40.0	31.0	40.0
33	35.47938293171212	37.0	34.0	40.0	30.0	40.0
34	35.02582849157712	37.0	34.0	40.0	27.0	40.0
35	35.00681638634332	36.0	34.0	40.0	28.0	40.0
36	34.966109628389866	36.0	34.0	40.0	29.0	40.0
37	34.63195126089616	35.0	34.0	39.0	28.0	40.0
38	34.57933412389138	35.0	34.0	39.0	28.0	40.0
39	34.15283319515433	35.0	33.0	39.0	26.0	40.0
40	34.066481711540135	35.0	33.0	39.0	26.0	40.0
41	33.98187709607116	35.0	33.0	39.0	26.0	40.0
42	33.9054271990406	35.0	33.0	39.0	26.0	40.0
43	33.82866501667679	35.0	33.0	38.0	26.0	40.0
44	34.09579076082544	35.0	33.0	39.0	27.0	40.0
45	34.16428750112665	35.0	34.0	39.0	27.0	40.0
46	34.091717284581364	35.0	34.0	38.0	27.0	40.0
47	33.79246556918885	35.0	33.0	38.0	26.0	40.0
48	33.744458817989425	35.0	33.0	38.0	26.0	40.0
49	33.694530464763886	35.0	33.0	37.0	27.0	40.0
50	33.2005077754812	35.0	33.0	37.0	24.0	40.0
51	33.248938712297196	35.0	33.0	37.0	24.0	40.0
52	32.93140547932504	35.0	33.0	36.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	6.0
12	7.0
13	15.0
14	71.0
15	182.0
16	497.0
17	1220.0
18	2641.0
19	4955.0
20	8621.0
21	14248.0
22	21937.0
23	32490.0
24	45581.0
25	61255.0
26	82951.0
27	107885.0
28	134000.0
29	160333.0
30	201131.0
31	268918.0
32	385175.0
33	588590.0
34	1008336.0
35	958055.0
36	1205818.0
37	1395648.0
38	1354073.0
39	453999.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	100.0	0.0	0.0
3	100.0	0.0	0.0	0.0
4	0.0	0.0	100.0	0.0
5	0.0	0.0	0.0	100.0
6	100.0	0.0	0.0	0.0
7	99.35682635264615	0.40385294678982675	0.16549710671286386	0.07382359385115592
8	98.27411168707269	1.2071934349951134	0.2450980968950554	0.2735967810371497
9	91.40369315647989	4.2043560391676875	2.5888383526866305	1.8031124516657844
10	51.66427844085134	28.413035123981047	8.668236016171061	11.25445041899655
11	35.510042903345216	27.147255830875487	19.14765636564353	18.195044900135763
12	36.33313949835256	17.028246173092676	24.687591117541423	21.95102321101334
13	25.18436483587135	16.860113349927364	28.675924306930124	29.279597507271166
14	23.542042854396197	20.731698420382184	30.255424457424823	25.470834267796793
15	26.583942038712554	20.09598479191607	27.891751595961612	25.42832157340976
16	29.94982254803652	29.84096981186868	22.064676716433855	18.144530923660945
17	33.437746142381876	19.549544291685326	24.627781533935202	22.384928031997596
18	30.94917091421002	20.463055374284682	24.935301397706315	23.652472313798985
19	30.061663998395975	22.866299282308532	23.424847604992706	23.647189114302787
20	27.913060892816	23.882520940414217	24.440869231046197	23.763548935723584
21	27.348229210374654	23.729614086398314	27.942736236088656	20.979420467138382
22	27.02303592646257	22.06214689930316	28.263975945322063	22.650841228912206
23	28.26618806448751	23.702327361160695	26.406690107285424	21.62479446706637
24	31.7451102164841	21.556101107024446	23.204553482569796	23.49423519392166
25	28.607066214609915	20.008770817159174	23.102019405933046	28.28214356229786
26	27.38176399559553	19.957209614058158	23.761607448158166	28.89941894218815
27	28.096031387617636	19.517256765142836	23.882391507909855	28.504320339329663
28	28.362074017036615	24.62035681482139	20.626716892753873	26.390852275388127
29	31.9877608623876	24.196700694864283	19.957750877258214	23.857787565489904
30	30.044920138968152	23.71147000260512	23.644659297172087	22.59895056125464
31	31.150873822370123	23.644188633519867	22.262390750141375	22.94254679396863
32	32.734868810743556	23.777857110751157	21.68008567961125	21.807188398894034
33	33.24572713886625	24.099579250228096	21.618122809796112	21.03657080110954
34	26.870034939716227	25.486648566511484	26.824380565450607	20.818935928321693
35	25.548552603370094	30.388669337369116	24.716713431022715	19.34606462823808
36	26.258219258191723	31.812109187378024	21.43761153257734	20.492060021852915
37	28.354261000409714	29.809305914665384	20.782471261865727	21.05396182305918
38	29.30963761487429	29.308719820752454	20.07995869455788	21.301683869815374
39	29.117548011810833	27.44657438050662	20.597335714263863	22.838541893418686
40	27.479026639327387	26.233768281458747	21.296400670319173	24.990804408894697
41	23.494588191660828	28.32858629818096	22.18947318382075	25.987352326337465
42	23.098960092193597	27.369232575855097	22.941217169151106	26.590590162800204
43	23.91672642133951	24.731233404693786	23.031643423334422	28.320396750632277
44	24.241307842503705	24.56723065507673	22.983012101468496	28.208449400951068
45	26.941234583706237	25.136215944248946	21.729952493564262	26.19259697848055
46	25.831927421782176	28.686996669348662	21.41276049173997	24.06831541712919
47	24.835556003208985	26.576634985511795	23.33005594543502	25.257753065844195
48	25.11503607989892	26.027288137228577	25.449089607064096	23.408586175808406
49	27.874760638116364	25.858696417002346	23.077744928069652	23.188798016811635
50	24.12286533442182	26.701066688568215	22.861121982134076	26.31494599487588
51	22.772896080524905	27.300633348543613	25.087949386713493	24.83852118421799
52	23.339657483940368	26.41823313335619	23.911149057060673	26.330960325642767
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	127.5
6	255.0
7	711.5
8	1168.0
9	11563.0
10	21958.0
11	27827.5
12	33697.0
13	25703.0
14	19289.0
15	20869.0
16	19320.5
17	17772.0
18	19263.5
19	20755.0
20	21184.5
21	21614.0
22	23506.5
23	25399.0
24	28761.5
25	32124.0
26	40220.5
27	48317.0
28	54298.5
29	60280.0
30	76816.0
31	93352.0
32	114643.5
33	135935.0
34	165868.5
35	195802.0
36	208174.5
37	220547.0
38	199635.0
39	182727.0
40	186731.0
41	202384.0
42	218037.0
43	227305.5
44	236574.0
45	250612.0
46	264650.0
47	279043.5
48	293437.0
49	306354.5
50	319272.0
51	338872.0
52	358472.0
53	402189.5
54	445907.0
55	502697.0
56	559487.0
57	632920.0
58	706353.0
59	719340.5
60	732328.0
61	758730.5
62	785133.0
63	750001.5
64	590222.5
65	465575.0
66	403468.5
67	341362.0
68	286128.5
69	230895.0
70	203350.5
71	175806.0
72	154892.0
73	133978.0
74	115901.0
75	97824.0
76	75514.5
77	53205.0
78	36585.5
79	19966.0
80	13902.5
81	7839.0
82	5716.5
83	3594.0
84	2145.5
85	697.0
86	449.5
87	202.0
88	131.5
89	49.5
90	38.0
91	37.5
92	37.0
93	34.5
94	32.0
95	16.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	8498638.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.418031153374175
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.90500704417914	7.491086034698477
2	9.858094045527425	2.054038617583943
3	4.124586462113556	1.2891021077125318
4	2.275191687269802	0.9481207151149899
5	1.2860029973952831	0.6698809645098313
6	0.810260873719803	0.5064793814863852
7	0.5708030135625963	0.41626505044144935
8	0.4178631510062405	0.34826490600240884
9	0.3374962153573224	0.3164441477164618
>10	4.109868303988332	11.103765502809743
>50	2.2962143090416647	17.218678793725832
>100	1.8704088180966831	34.486411285200695
>500	0.08775001395207678	6.1445032061197935
>1k	0.044960705838217575	8.556853522949435
>5k	0.0033209612266865253	2.506443236847143
>10k+	0.0021713977251411895	5.943662527080925
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TAGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	99006	1.164963138799417	No Hit
TAGTCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	54027	0.6357136284661142	No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAA	38558	0.4536962275602279	No Hit
TAGTCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	35038	0.412277826164616	No Hit
TAGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	30955	0.3642348338639674	No Hit
TAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	26055	0.3065785364666668	No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAA	22236	0.261641924270689	No Hit
TAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	19327	0.2274129101627814	No Hit
TAGTCGGGGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAA	18857	0.22188261224916275	No Hit
TAGTCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTAGTCGGGGAAG	18127	0.21329300059609552	No Hit
TAGTCGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	17079	0.20096161290785652	No Hit
TAGTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	12287	0.14457610737155766	No Hit
TAGTCGGGGGGGCTGATGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	11747	0.138222148066549	No Hit
TAGTCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT	11441	0.13462157112704412	No Hit
TAGTCGGGGGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAA	10987	0.12927953867431463	No Hit
TAGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC	10502	0.12357274189111243	No Hit
TAGTCGGGGAGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAA	10432	0.12274908049972241	No Hit
TAGTCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	9673	0.11381823769879362	No Hit
TAGTCGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	9493	0.11170025126379074	No Hit
TAGTCGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT	9457	0.11127665397679017	No Hit
TAGTCGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	8591	0.10108678590616521	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.001812055061058019	0.0	0.0	0.0	0.0
8	0.002859281687253887	0.0	0.0	0.0	0.0
9	0.0065892911311200685	0.0	0.0	0.0	0.0
10	0.021356363219612366	0.0	0.0	0.0	0.0
11	0.043889385569781884	0.0	0.0	0.0	0.0
12	0.05207893311845969	0.0	0.0	0.0	0.0
13	0.05983311678883134	0.0	0.0	0.0	0.0
14	0.08996735712240009	0.0	0.0	0.0	0.0
15	0.10602875425450525	0.0	0.0	0.0	0.0
16	0.12969136937000963	0.0	0.0	0.0	0.0
17	0.14117556248424748	0.0	0.0	0.0	0.0
18	0.1527538883289299	0.0	0.0	0.0	0.0
19	0.16890941819147962	0.0	0.0	0.0	0.0
20	0.18134670520146876	0.0	0.0	0.0	0.0
21	0.19404285722018047	0.0	0.0	0.0	0.0
22	0.2062683455866693	0.0	0.0	0.0	0.0
23	0.21974109263154873	0.0	0.0	0.0	0.0
24	0.23247254442417714	0.0	0.0	0.0	0.0
25	0.24339194115574755	0.0	0.0	0.0	0.0
26	0.2556056629309308	0.0	0.0	0.0	0.0
27	0.26886661133230993	0.0	0.0	0.0	0.0
28	0.3036015888663572	0.0	0.0	3.529977391671466E-5	0.0
29	0.31561527858934574	0.0	0.0	3.529977391671466E-5	0.0
30	0.3341005935303986	0.0	0.0	3.529977391671466E-5	0.0
31	0.3491618304015302	0.0	0.0	3.529977391671466E-5	0.0
32	0.3643642663683287	0.0	0.0	3.529977391671466E-5	0.0
33	0.3792019380046544	0.0	0.0	3.529977391671466E-5	0.0
34	0.39916984345020934	0.0	0.0	3.529977391671466E-5	0.0
35	0.45330793004714404	0.0	0.0	3.529977391671466E-5	0.0
36	0.4742406959797558	0.0	0.0	3.529977391671466E-5	0.0
37	0.49360850526872657	0.0	0.0	3.529977391671466E-5	0.0
38	0.5115407904184176	0.0	0.0	3.529977391671466E-5	0.0
39	0.5311203983508886	0.0	0.0	3.529977391671466E-5	0.0
40	0.5520531642835005	0.0	0.0	3.529977391671466E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGGGG	175965	0.0	43.285202	5
TCGGGGG	432670	0.0	42.75522	4
CGGGGGC	82185	0.0	42.510193	5
CGGGGAT	54145	0.0	42.457287	5
TCGGGGC	92995	0.0	41.72869	4
CGGGGGT	66330	0.0	41.669075	5
GTCGGGG	843605	0.0	41.547523	3
AGTCGGG	928355	0.0	41.121304	2
CGGGGAC	37130	0.0	41.038242	5
TAGTCGG	946695	0.0	41.01539	1
CGGGGAG	82090	0.0	40.8755	5
CGGGGGA	113315	0.0	40.811985	5
TCGGGGT	72050	0.0	39.915615	4
CGGGGCA	40005	0.0	39.537807	5
CGGGGCT	34825	0.0	39.402153	5
TCGGGAT	7025	0.0	39.288258	4
GTCGGGT	24445	0.0	39.197384	3
TCGGGGA	251780	0.0	38.90031	4
CGGGGTC	14395	0.0	38.474472	5
CGGGGTG	33090	0.0	38.31248	5
>>END_MODULE