FastQCFastQC Report
Fri 17 Jun 2016
SRR1527088_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527088_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8297135
Sequences flagged as poor quality0
Sequence length52
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC925811.1158188941122449No Hit
TAGTCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA529820.6385577672292906No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAA350500.42243497303587324No Hit
TAGTCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT341710.41184095473919613No Hit
TAGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC291470.3512899332118858No Hit
TAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA236010.2844475834128286No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAA203790.2456149020113569No Hit
TAGTCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTAGTCGGGGAAG185450.223510886589166No Hit
TAGTCGGGGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAA183800.22152224834234951No Hit
TAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA179200.21597816595728525No Hit
TAGTCGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC169910.2047815300100577No Hit
TAGTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT119490.1440135661285492No Hit
TAGTCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT113430.13670984020387758No Hit
TAGTCGGGGGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAA111690.134612730779962No Hit
TAGTCGGGGAGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAA105410.1270438530890482No Hit
TAGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC99160.11951113245716745No Hit
TAGTCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC98490.11870362480542983No Hit
TAGTCGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC92990.11207483064937475No Hit
TAGTCGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT91700.11052007711095456No Hit
TAGTCGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG83830.10103487529129031No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGGG1699300.043.3051835
TCGGGGG4226650.042.765484
CGGGGAT519850.042.655195
CGGGGGC817000.042.4641385
TCGGGGC908600.041.7068024
CGGGGGT655450.041.676865
GTCGGGG8230400.041.597523
AGTCGGG9049800.041.2219962
TAGTCGG9209200.041.1773221
CGGGGAC366150.041.1757475
CGGGGGA1105950.040.8299645
CGGGGAG813450.040.8002935
TCGGGGT690600.039.8753284
TCGGGAT67650.039.7442744
CGGGGCA390350.039.624695
CGGGGCT339400.039.474075
GTCGGGT243650.039.2977643
TCGGGGA2465300.039.012214
CGGGGTG316900.038.546235
CGGGGTC137050.037.8102875
Produced by FastQC (version 0.11.5)