##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527088_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8297135
Sequences flagged as poor quality	0
Sequence length	52
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.273330131424885	33.0	31.0	34.0	31.0	34.0
2	32.594184378101595	34.0	31.0	34.0	31.0	34.0
3	32.88303830177525	34.0	31.0	34.0	31.0	34.0
4	35.810314162659765	37.0	35.0	37.0	35.0	37.0
5	36.21568577587324	37.0	35.0	37.0	35.0	37.0
6	36.262215330954604	37.0	37.0	37.0	35.0	37.0
7	36.61666695793186	37.0	37.0	37.0	35.0	37.0
8	36.64605637970215	37.0	37.0	37.0	35.0	37.0
9	38.45723035722572	39.0	39.0	39.0	37.0	39.0
10	37.89576835859607	39.0	38.0	39.0	35.0	39.0
11	36.654358643073785	39.0	35.0	39.0	33.0	39.0
12	36.5992834876135	39.0	35.0	39.0	33.0	39.0
13	36.39299288248293	39.0	35.0	39.0	33.0	39.0
14	37.135690813756796	40.0	35.0	40.0	33.0	40.0
15	37.333918997340646	40.0	35.0	40.0	33.0	40.0
16	37.40547429926113	40.0	35.0	40.0	33.0	40.0
17	37.30749023608752	40.0	35.0	40.0	33.0	40.0
18	37.276266205141894	40.0	35.0	40.0	33.0	40.0
19	37.20045907412619	40.0	35.0	40.0	33.0	40.0
20	37.170685302818384	40.0	35.0	40.0	33.0	40.0
21	32.90141826064057	38.0	33.0	40.0	10.0	40.0
22	33.50288949137263	38.0	33.0	40.0	16.0	40.0
23	35.4625867844744	38.0	33.0	40.0	27.0	40.0
24	36.323077905807246	38.0	35.0	40.0	31.0	40.0
25	36.64649195173997	38.0	35.0	40.0	32.0	40.0
26	36.73287357624047	38.0	35.0	40.0	32.0	40.0
27	36.685396947259505	38.0	35.0	40.0	32.0	40.0
28	36.538173477953535	38.0	35.0	40.0	32.0	40.0
29	36.42132663865298	38.0	35.0	40.0	32.0	40.0
30	36.199204665224805	38.0	35.0	40.0	31.0	40.0
31	35.92057619889275	38.0	35.0	40.0	31.0	40.0
32	35.84785374710668	37.0	35.0	40.0	31.0	40.0
33	35.51113848334395	37.0	34.0	40.0	30.0	40.0
34	35.05059360851668	37.0	34.0	40.0	27.0	40.0
35	35.03439307664634	36.0	34.0	40.0	28.0	40.0
36	34.930457923126475	36.0	34.0	40.0	29.0	40.0
37	34.80429304814252	35.0	34.0	39.0	29.0	40.0
38	34.687527682748325	35.0	34.0	39.0	29.0	40.0
39	34.45247498082169	35.0	34.0	39.0	28.0	40.0
40	29.780704544399963	35.0	27.0	38.0	8.0	40.0
41	30.496101967727416	35.0	27.0	38.0	11.0	40.0
42	32.15071624120856	35.0	29.0	38.0	21.0	40.0
43	33.0451235275791	35.0	31.0	37.0	24.0	40.0
44	33.78654077582202	35.0	33.0	38.0	26.0	40.0
45	34.03892415876083	35.0	33.0	39.0	27.0	40.0
46	34.06426157944881	35.0	34.0	38.0	27.0	40.0
47	34.006211300647756	35.0	34.0	38.0	27.0	40.0
48	33.86717186113038	35.0	33.0	38.0	26.0	40.0
49	33.75109914446372	35.0	33.0	37.0	27.0	40.0
50	33.62482615987326	35.0	33.0	37.0	27.0	40.0
51	33.44003622937315	35.0	33.0	37.0	26.0	40.0
52	33.061595719486306	35.0	33.0	36.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	7.0
12	8.0
13	36.0
14	49.0
15	202.0
16	566.0
17	1394.0
18	2943.0
19	5310.0
20	9190.0
21	15168.0
22	23339.0
23	33836.0
24	47553.0
25	63594.0
26	85628.0
27	111638.0
28	141424.0
29	171861.0
30	220759.0
31	316177.0
32	432158.0
33	539638.0
34	1065370.0
35	941904.0
36	1126466.0
37	1139891.0
38	1329583.0
39	471443.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	100.0	0.0	0.0
3	100.0	0.0	0.0	0.0
4	0.0	0.0	100.0	0.0
5	0.0	0.0	0.0	100.0
6	100.0	0.0	0.0	0.0
7	99.35552452744231	0.4056219405855154	0.16534623095803552	0.07350730101414524
8	98.28123804180599	1.2170827641107442	0.2322729472281697	0.2694062468550891
9	91.34070977512117	4.229230933328191	2.6266295534542947	1.8034297380963429
10	51.57790008237784	28.495751846872448	8.67423514261248	11.252112928137242
11	35.53962904062667	27.224662488919364	19.116670995470123	18.119037474983834
12	36.35782712948506	17.07966665602042	24.658704480522495	21.903801733972028
13	25.27749638881373	16.9218893027533	28.639427947116687	29.16118636131629
14	23.548104255264015	20.803723212892162	30.18423829430279	25.463934237541032
15	26.540546827308464	19.997794419398986	27.919842210594382	25.54181654269817
16	31.620396679094654	22.343218472400412	25.031158345621712	21.00522650288322
17	33.45246280794515	19.583615308175652	24.578194762408952	22.385727121470243
18	30.96654447589439	20.490542819901087	24.900968828396792	23.641943875807733
19	30.07779191250956	22.869894246628505	23.426749112796163	23.625564728065772
20	27.867052904406158	23.927825689228875	24.423093031510273	23.782028374854693
21	32.15854629338922	22.540792695309886	26.261558959809623	19.03910205149127
22	27.21015145589411	22.040053584761488	28.146161295435114	22.60363366390929
23	28.204651364597538	23.80598845264058	26.368957477490724	21.620402705271157
24	31.825816983814292	21.607615158726475	23.182074294319666	23.384493563139568
25	28.654396969556363	20.026695961919387	23.062527004803464	28.256380063720794
26	27.442737764300567	19.96547000862346	23.706592697358786	28.885199529717188
27	28.148005305445796	19.527137981966064	23.854053236448486	28.470803476139654
28	28.405202518700733	24.651448964009866	20.591927213429695	26.351421303859706
29	31.874990584099212	24.244356636357008	19.940461376125615	23.94019140341817
30	30.02379737102024	23.801878600263827	23.591757877869888	22.582566150846045
31	31.162347002911243	23.702784153807308	22.254886777182726	22.87998206609872
32	32.68964528117236	23.8325036292648	21.695175503351457	21.782675586211386
33	33.19336132291448	24.163738447066365	21.58389612800081	21.059004102018346
34	26.85353438265136	25.556050371604172	26.744882420257117	20.84553282548735
35	25.539490438567046	30.439458921663924	24.668973085287874	19.352077554481156
36	26.354277711523316	31.770918515849147	21.419188671752355	20.455615100875182
37	28.226924112961882	29.858439087709193	20.788223886920004	21.12641291240892
38	29.35049267006021	29.298667552112867	20.047438061451334	21.303401716375593
39	28.86133587075539	27.508085622326263	20.576825615106902	23.05375289181145
40	28.045777247206416	30.10921239680926	19.709188774197358	22.135821581786967
41	23.519528126274913	28.39122178920796	22.17409985494993	25.915150229567196
42	23.11979978631178	27.456176137907846	22.891865686167577	26.532158389612796
43	23.92436666391471	24.864498408185476	22.990875766153014	28.220259161746796
44	24.266569122956298	24.686521311271903	22.958165680081137	28.088743885690665
45	26.87336050335447	25.22899772029743	21.693777430402182	26.20386434594592
46	25.813054747210934	28.745524810672602	21.374534703846567	24.066885738269896
47	24.689221038346368	26.677811075750846	23.275070250152613	25.35789763575017
48	25.097759648360547	26.116364263085995	25.375445861734203	23.41043022681926
49	27.868667919709633	25.960177820416323	23.018668492196404	23.15248576767764
50	24.04074418458902	26.813725460655995	22.9063405621338	26.239189792621186
51	22.82913318874527	27.412052473534537	24.994097360112857	24.764716977607332
52	23.320820982182404	26.53264048373324	23.873337001266098	26.27320153281826
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	120.5
6	241.0
7	639.0
8	1037.0
9	10769.5
10	20502.0
11	25864.5
12	31227.0
13	24736.5
14	19736.0
15	21226.0
16	19397.0
17	17568.0
18	18985.5
19	20403.0
20	20715.5
21	21028.0
22	22973.0
23	24918.0
24	28039.5
25	31161.0
26	38582.5
27	46004.0
28	51509.0
29	57014.0
30	71789.5
31	86565.0
32	106953.0
33	127341.0
34	156882.5
35	186424.0
36	200537.5
37	214651.0
38	196947.0
39	182081.0
40	184919.0
41	198890.0
42	212861.0
43	222299.0
44	231737.0
45	244568.0
46	257399.0
47	271400.0
48	285401.0
49	297885.5
50	310370.0
51	331158.5
52	351947.0
53	393254.0
54	434561.0
55	483992.5
56	533424.0
57	613309.0
58	693194.0
59	703619.0
60	714044.0
61	735567.0
62	757090.0
63	732996.5
64	586101.5
65	463300.0
66	401436.5
67	339573.0
68	283893.0
69	228213.0
70	200667.0
71	173121.0
72	151984.5
73	130848.0
74	113284.5
75	95721.0
76	74454.5
77	53188.0
78	36642.0
79	20096.0
80	13958.5
81	7821.0
82	5699.5
83	3578.0
84	2143.0
85	708.0
86	450.5
87	193.0
88	118.5
89	40.5
90	37.0
91	30.5
92	24.0
93	22.5
94	21.0
95	10.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	8297135.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.50676149226042
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.53638025432848	7.516156853518771
2	9.932473509420827	2.0871626038335895
3	4.12688157150092	1.3008048113569513
4	2.238275172356432	0.9406809355998847
5	1.3126082100767762	0.6895630698029773
6	0.8027052040824748	0.5060299276734472
7	0.597829120517078	0.4396873587680525
8	0.45056371117453836	0.37871723603028684
9	0.34678162229653126	0.32791966158319125
>10	4.347493333017507	11.885109427940806
>50	2.3568712582391025	17.781219228613967
>100	1.8192690997930232	33.75293049907891
>500	0.0833057901331998	5.924927897036128
>1k	0.04329795411325043	8.320938805022712
>5k	0.0032901180937120393	2.5515276132396085
>10k+	0.0019740708562272233	5.596624070900835
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TAGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	92581	1.1158188941122449	No Hit
TAGTCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	52982	0.6385577672292906	No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAA	35050	0.42243497303587324	No Hit
TAGTCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	34171	0.41184095473919613	No Hit
TAGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	29147	0.3512899332118858	No Hit
TAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	23601	0.2844475834128286	No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAA	20379	0.2456149020113569	No Hit
TAGTCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTAGTCGGGGAAG	18545	0.223510886589166	No Hit
TAGTCGGGGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAA	18380	0.22152224834234951	No Hit
TAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	17920	0.21597816595728525	No Hit
TAGTCGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	16991	0.2047815300100577	No Hit
TAGTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	11949	0.1440135661285492	No Hit
TAGTCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT	11343	0.13670984020387758	No Hit
TAGTCGGGGGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAA	11169	0.134612730779962	No Hit
TAGTCGGGGAGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAA	10541	0.1270438530890482	No Hit
TAGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC	9916	0.11951113245716745	No Hit
TAGTCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	9849	0.11870362480542983	No Hit
TAGTCGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	9299	0.11207483064937475	No Hit
TAGTCGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT	9170	0.11052007711095456	No Hit
TAGTCGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	8383	0.10103487529129031	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0023863658961798258	0.0	0.0	0.0	0.0
8	0.0038205959044899235	0.0	0.0	0.0	0.0
9	0.007966605340277096	0.0	0.0	0.0	0.0
10	0.024876056614723033	0.0	0.0	0.0	0.0
11	0.04735369498025523	0.0	0.0	0.0	0.0
12	0.056658111504754353	0.0	0.0	0.0	0.0
13	0.0650827062594498	0.0	0.0	0.0	0.0
14	0.09363473054253064	0.0	0.0	0.0	0.0
15	0.11142400358678026	0.0	0.0	0.0	0.0
16	0.13286513959336566	0.0	0.0	0.0	0.0
17	0.1431096396527235	0.0	0.0	0.0	0.0
18	0.15369160559638959	0.0	0.0	0.0	0.0
19	0.16739513096990707	0.0	0.0	0.0	0.0
20	0.1794595363339273	0.0	0.0	0.0	0.0
21	0.19087311463535306	0.0	0.0	0.0	0.0
22	0.20321472411862648	0.0	0.0	0.0	0.0
23	0.21805117067517885	0.0	0.0	0.0	0.0
24	0.2309471883969587	0.0	0.0	0.0	0.0
25	0.24239692375741748	0.0	0.0	0.0	0.0
26	0.25429259617928357	0.0	0.0	0.0	0.0
27	0.26851437273227446	0.0	0.0	0.0	0.0
28	0.3056838294182269	0.0	0.0	0.0	0.0
29	0.31741076889793884	0.0	0.0	0.0	0.0
30	0.3348866807639022	0.0	0.0	0.0	0.0
31	0.3491807714349592	0.0	0.0	4.941464734513781E-4	0.0
32	0.3636676997541923	0.0	0.0	4.941464734513781E-4	0.0
33	0.3778412668951391	0.0	0.0	4.941464734513781E-4	0.0
34	0.3956184875863777	0.0	0.0	4.941464734513781E-4	0.0
35	0.4435868525701944	0.0	0.0	4.941464734513781E-4	0.0
36	0.4615207538505761	0.0	0.0	4.941464734513781E-4	0.0
37	0.4789725610105175	0.0	0.0	4.941464734513781E-4	0.0
38	0.49632794934637076	0.0	0.0	4.941464734513781E-4	0.0
39	0.51494883474838	0.0	0.0	4.941464734513781E-4	0.0
40	0.5387642842981343	0.0	0.0	4.941464734513781E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGGGG	169930	0.0	43.305183	5
TCGGGGG	422665	0.0	42.76548	4
CGGGGAT	51985	0.0	42.65519	5
CGGGGGC	81700	0.0	42.464138	5
TCGGGGC	90860	0.0	41.706802	4
CGGGGGT	65545	0.0	41.67686	5
GTCGGGG	823040	0.0	41.59752	3
AGTCGGG	904980	0.0	41.221996	2
TAGTCGG	920920	0.0	41.177322	1
CGGGGAC	36615	0.0	41.175747	5
CGGGGGA	110595	0.0	40.829964	5
CGGGGAG	81345	0.0	40.800293	5
TCGGGGT	69060	0.0	39.875328	4
TCGGGAT	6765	0.0	39.744274	4
CGGGGCA	39035	0.0	39.62469	5
CGGGGCT	33940	0.0	39.47407	5
GTCGGGT	24365	0.0	39.297764	3
TCGGGGA	246530	0.0	39.01221	4
CGGGGTG	31690	0.0	38.54623	5
CGGGGTC	13705	0.0	37.810287	5
>>END_MODULE