FastQCFastQC Report
Fri 17 Jun 2016
SRR1527084_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527084_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4798164
Sequences flagged as poor quality0
Sequence length50
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1055122.19900778714525No Hit
AATAAAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAATAAAGGGA738851.5398598297182007No Hit
AATAAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA435640.9079306167942571No Hit
AATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA415870.8667273565472127No Hit
AATAAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA319640.6661714772567173No Hit
AATAAAGGGAACGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAA288810.6019177335330763No Hit
AATAAAGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA281910.5875372329916193Illumina Single End Adapter 2 (97% over 34bp)
AATAAAGGGAACGCAGAGTGCAGTGCTAATAAAGGGAACGCAGAGTGCAG256670.5349337788370718No Hit
AATAAAGGGGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAAA239410.4989616861782965No Hit
AATAAAGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA231240.481934339885006Illumina Single End Adapter 2 (97% over 34bp)
AATAAAGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA228650.4765364418556765No Hit
AATAAAGGGGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAA204960.4271633899966737No Hit
AATAAAGGGGAGTGCAGTGCTAATAAAGGGGAGTGCAGTGCTAATAAAGG185880.38739817980377494No Hit
AATAAAGGGACGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAA166690.34740371525441816No Hit
AATAAAGGGCGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAA159720.3328773255770332No Hit
AATAAAGGGGCAGAGTGCAGTGCTAATAAAGGGGCAGAGTGCAGTGCTAA159590.3326063886103101No Hit
AATAAAGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA141440.29477941979473815Illumina Single End Adapter 2 (97% over 34bp)
AATAAAGGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAA133580.2783981539605566Illumina Single End Adapter 2 (97% over 34bp)
AATAAAGGGAGAGTGCAGTGCTAATAAAGGGAGAGTGCAGTGCTAATAAA133460.27814805829896605No Hit
AATAAAGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA98470.2052243316401857No Hit
AATAAAGGGGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAAAAA97800.20382796419630508No Hit
AATAAAGGGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAA97500.20320272504232867No Hit
AATAAAGGGAACGCAGAGTGCAGTGCTAATAAAGGGGAAAAAAAAAAAAA94880.1977423030976015No Hit
AATAAAGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA92110.19196926157588612Illumina Single End Adapter 2 (97% over 34bp)
AATAAAGGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA88310.1840495656255184No Hit
AATAAAGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA86900.18111094160182936Illumina Single End Adapter 2 (100% over 34bp)
AATAAAGGGAACGCAGAGTGCAGTGCTAATAAAGGGGGGAAAAAAAAAAA86340.17994382851440677No Hit
AATAAAGGAGAGTGCAGTGCTAATAAAGGGGAGTGCAGTGCTAATAAAGG84020.17510864572365595No Hit
AATAAAGGGGAGTGCAGTGCTAATAAAGGGGAAAAAAAAAAAAAAAAAAA80420.16760577587593922No Hit
AATAAAGGGGAGTGCAGTGCTAATAAAGGGGGGAAAAAAAAAAAAAAAAA79930.16658455192444444No Hit
AATAAAGGGAACGCAGAGTGCAGTGCTAATAAAGGGGGTAAAAAAAAAAA78180.1629373235262488No Hit
AATAAAGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA75590.15753942549691924No Hit
AATAAAGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA72510.1511203035160949No Hit
AATAAAGGGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAAAA71950.1499531904286723No Hit
AATAAAGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA71850.14974477737734684No Hit
AATAAAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA71350.1487027121207195Illumina Single End Adapter 2 (100% over 34bp)
AATAAAGGGAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAA70600.1471396142357785No Hit
AATAAAGGGGCAGAGTGCAGTGCTAATAAAGGGGGGAAAAAAAAAAAAAA68830.1434507032273178No Hit
AATAAAGGGGCAGAGTGCAGTGCTAATAAAGGGGAAAAAAAAAAAAAAAA66560.1387197269622297No Hit
AATAAAGGGGAGTGCAGTGCTAATAAAGGGGGTAAAAAAAAAAAAAAAAA65930.1374067247388793No Hit
AATAAAGGGCGCAGAGTGCAGTGCTAATAAAGGGCGCAGAGTGCAGTGCT61340.12784056568304045No Hit
AATAAAGGGGCAGAGTGCAGTGCTAATAAAGGGGGTAAAAAAAAAAAAAA56150.11702392831924877No Hit
AATAAAGGGACGCAGAGTGCAGTGCTAATAAAGGGGAAAAAAAAAAAAAA54820.11425203473662009No Hit
AATAAAGGGCGCAGAGTGCAGTGCTAATAAAGGGGAAAAAAAAAAAAAAA51840.10804132580712122No Hit
AATAAAGGGACGCAGAGTGCAGTGCTAATAAAGGGGGGAAAAAAAAAAAA51230.10677000619403589No Hit
AATAAAGGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAA51120.10654075183757787Illumina Single End Adapter 2 (100% over 34bp)
AATAAAGGGACGCAGAGTGCAGTGCTAATAAAGGGGGTAAAAAAAAAAAA50450.10514438439369726No Hit
AATAAAGGGCGCAGAGTGCAGTGCTAATAAAGGGGGTAAAAAAAAAAAAA49710.1036021278138888No Hit
AATAAAGGGCGCAGAGTGCAGTGCTAATAAAGGGGGGAAAAAAAAAAAAA48970.10205987123408036No Hit
AATAAAGGGAAACGCAGAGTGCAGTGCTAATAAAGGGAAACGCAGAGTGC48770.10164304513142944No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACTA207.858983E-444.0000049
GGTACGT207.858983E-444.0000049
TAGGTCC408.3218765E-944.00000439
CTATAAC408.3218765E-944.00000442
CGCGCAC408.3218765E-944.00000443
TAATTAC207.858983E-444.00000421
TTCGCCC207.858983E-444.00000419
TATCGTA207.858983E-444.00000435
TGATCGC254.4449596E-544.018
CTTAACG254.4449596E-544.019
CCTTACG254.4449596E-544.044
ATGATTA254.4449596E-544.017
CTATCTA302.5294048E-644.017
TCACACG254.4449596E-544.021
TTAGACT454.802132E-1044.037
ATAGCTA302.5294048E-644.013
TTACTCA302.5294048E-644.041
TTATAGA502.7284841E-1144.013
TACGCGC254.4449596E-544.038
CCATACT1450.044.030
Produced by FastQC (version 0.11.5)