##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527084_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4798164
Sequences flagged as poor quality	0
Sequence length	50
%GC	34
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.86809537981611	33.0	31.0	34.0	30.0	34.0
2	32.26957019393251	34.0	31.0	34.0	30.0	34.0
3	32.32348665031041	34.0	31.0	34.0	30.0	34.0
4	35.9657231391007	37.0	35.0	37.0	35.0	37.0
5	36.07982907628835	37.0	35.0	37.0	35.0	37.0
6	36.20994655455712	37.0	36.0	37.0	35.0	37.0
7	36.344954653488294	37.0	36.0	37.0	35.0	37.0
8	36.51006655879207	37.0	37.0	37.0	35.0	37.0
9	38.52074501830283	39.0	39.0	39.0	37.0	39.0
10	37.80642762523332	39.0	38.0	39.0	35.0	39.0
11	37.4041076961938	39.0	37.0	39.0	35.0	39.0
12	36.22767354346371	38.0	35.0	39.0	32.0	39.0
13	35.77584300995131	38.0	35.0	39.0	31.0	39.0
14	36.7098100440085	39.0	35.0	40.0	31.0	40.0
15	37.050473889596105	40.0	35.0	40.0	32.0	40.0
16	36.69719188422905	39.0	35.0	40.0	31.0	40.0
17	37.008851719115896	39.0	35.0	40.0	32.0	40.0
18	37.052063455938566	39.0	35.0	40.0	32.0	40.0
19	36.64265248124074	39.0	35.0	40.0	31.0	40.0
20	36.797559233073315	39.0	35.0	40.0	31.0	40.0
21	37.02714871771786	40.0	35.0	40.0	32.0	40.0
22	36.94864389795764	40.0	35.0	40.0	32.0	40.0
23	37.03872793843645	40.0	35.0	40.0	32.0	40.0
24	36.767745537668155	39.0	35.0	40.0	32.0	40.0
25	36.231404345495484	37.0	35.0	40.0	31.0	40.0
26	35.62501323422876	36.0	35.0	40.0	31.0	40.0
27	35.43612681850808	35.0	35.0	40.0	30.0	40.0
28	35.20732263424093	35.0	35.0	40.0	30.0	40.0
29	35.29693149296273	35.0	35.0	40.0	30.0	40.0
30	34.99763055201948	35.0	35.0	40.0	27.0	40.0
31	34.785798693000075	35.0	35.0	40.0	25.0	40.0
32	33.657881431314145	35.0	34.0	40.0	19.0	40.0
33	33.74648678119381	35.0	33.0	40.0	21.0	40.0
34	33.53988233832774	35.0	33.0	40.0	20.0	40.0
35	33.37398700836403	35.0	33.0	40.0	18.0	40.0
36	32.79699005703015	35.0	33.0	40.0	15.0	40.0
37	32.04699943561746	35.0	31.0	40.0	12.0	40.0
38	31.201414124235853	35.0	28.0	40.0	10.0	40.0
39	30.443375007607077	35.0	24.0	40.0	9.0	40.0
40	29.612939866165476	35.0	21.0	40.0	8.0	40.0
41	28.894233085821995	35.0	17.0	39.0	8.0	40.0
42	28.363557602449603	35.0	15.0	39.0	8.0	40.0
43	27.90167801684144	35.0	14.0	39.0	8.0	40.0
44	27.44693407728456	35.0	10.0	38.0	8.0	40.0
45	27.175011108415635	35.0	10.0	38.0	8.0	40.0
46	26.66618127267013	34.0	10.0	37.0	8.0	40.0
47	26.437075723130764	34.0	10.0	36.0	8.0	40.0
48	26.2404242539438	33.0	10.0	35.0	8.0	40.0
49	25.94265848353662	33.0	10.0	35.0	8.0	40.0
50	25.502580987227613	33.0	10.0	35.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	5.0
11	18.0
12	45.0
13	53.0
14	125.0
15	490.0
16	1686.0
17	4316.0
18	8332.0
19	14252.0
20	21420.0
21	28192.0
22	36708.0
23	50458.0
24	69999.0
25	95826.0
26	128333.0
27	179816.0
28	250005.0
29	316946.0
30	318350.0
31	240127.0
32	187144.0
33	188420.0
34	270760.0
35	307204.0
36	526757.0
37	586325.0
38	841158.0
39	124890.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	0.0	100.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	100.0	0.0	0.0
6	0.0	100.0	0.0	0.0
7	99.31796828953742	0.5660290060948312	0.07244437664073175	0.043558327727022256
8	98.54315108862474	1.0130541598828218	0.26924882100736863	0.1745459304850772
9	96.44118041817661	2.043323237805127	0.7816323076910251	0.7338640363272285
10	62.592316561084616	25.6976001653966	4.363752468652593	7.346330804866194
11	47.40023475646101	33.58686781027076	8.75601584272651	10.256881590541715
12	37.85074874472819	39.152538345917314	10.164200306617282	12.832512602737214
13	24.801319838171434	46.71238832186645	12.17651168238518	16.309780157576938
14	21.169097179671223	57.34910269844883	8.994002706035058	12.487797415844893
15	25.951447261911014	54.60667872127756	8.26397346985222	11.177900546959211
16	22.032427403481833	54.37344367553922	15.904729392325898	7.689399528653043
17	27.677524152988518	54.309544234002836	9.22006834280779	8.79286327020085
18	24.83089365015452	52.49282850690389	12.114050290902938	10.562227552038655
19	19.39666922597894	53.21681376459829	17.89521992162002	9.491297087802751
20	20.289781674823953	56.93123453054126	9.93790541548809	12.841078379146689
21	17.288404481380795	52.031131074302586	18.092482874699574	12.587981569617046
22	17.032556619573654	56.57232641485368	11.909763817993715	14.48535314757895
23	17.562551009094314	59.48141830916992	12.107506121091317	10.848524560644446
24	14.57071913340186	56.9867766087195	18.75258953216272	9.68991472571592
25	17.35505497519468	57.811383687593846	12.179116845526746	12.654444491684735
26	18.715721263383244	57.783935688734275	12.630893816884958	10.869449230997523
27	14.878962036312224	57.41739965536817	16.24560977907383	11.45802852924577
28	19.302925035492745	61.591183627737614	12.088144548623182	7.017746788146466
29	23.820361288192736	57.1988577297483	11.4616340750337	7.51914690702527
30	20.31685453019113	58.29044192737055	12.957247813955505	8.435455728482811
31	25.84696979928156	54.95606236051957	10.6581809208689	8.538786919329977
32	22.58663938956651	54.64811123588106	14.385002263365736	8.380247111186694
33	26.721283390897018	53.45692644103036	10.668288953858184	9.153501214214437
34	24.920677992665528	51.17159396802611	11.792885778810396	12.114842260497975
35	25.402841586906995	53.3579510829559	11.944943941057454	9.294263389079656
36	26.981278672425535	50.93846312881344	12.172260056138139	9.907998142622887
37	23.078598397220272	51.87603008150617	14.6323468726788	10.413024648594755
38	25.353176756776136	51.30508252740006	12.934364060919968	10.407376654903834
39	22.895319959884656	50.11889964578118	12.728514490125805	14.25726590420836
40	23.953287132328114	49.289644955862286	15.81717506946407	10.93989284234553
41	23.63358151159485	50.177442871898506	13.601765175179507	12.58721044132714
42	24.5187742644895	50.29004844352964	14.044497020110192	11.146680271870657
43	25.766105535367277	47.83844403817793	15.451847831795662	10.943602594659124
44	25.015985281036663	52.52567440379279	12.183868663096968	10.274471652073585
45	24.64471827140548	52.531905954027415	12.662093250668379	10.161282523898725
46	24.00341047117189	53.49258591411215	12.5829588150801	9.921044799635862
47	26.237952683568132	50.7770055379516	12.013470152333268	10.971571626147002
48	25.205224331640185	51.62193288933017	11.847885983055185	11.32495679597446
49	24.952586030823458	52.29335637548029	11.489769836962637	11.264287756733617
50	22.306157105092698	54.586400131383584	11.729778306869045	11.37766445665467
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1465.0
1	1046.5
2	628.0
3	1814.5
4	3001.0
5	29130.5
6	55260.0
7	60514.5
8	65769.0
9	107982.5
10	150196.0
11	116070.0
12	81944.0
13	67866.0
14	53788.0
15	60292.0
16	66796.0
17	80766.5
18	94737.0
19	118596.0
20	142455.0
21	164468.0
22	186481.0
23	204129.5
24	221778.0
25	232592.0
26	243406.0
27	242306.0
28	241206.0
29	234419.5
30	227633.0
31	212014.5
32	196396.0
33	201423.0
34	206450.0
35	228579.0
36	250708.0
37	226899.0
38	203090.0
39	208252.0
40	213414.0
41	242282.0
42	271150.0
43	332741.5
44	394333.0
45	353751.5
46	313170.0
47	323489.0
48	333808.0
49	300070.5
50	266333.0
51	206253.0
52	146173.0
53	108597.0
54	71021.0
55	53893.5
56	36766.0
57	29182.5
58	21599.0
59	17140.0
60	12681.0
61	10708.0
62	8735.0
63	7033.5
64	5332.0
65	4637.5
66	3943.0
67	3113.5
68	2284.0
69	1788.5
70	1293.0
71	1644.5
72	1996.0
73	1258.5
74	521.0
75	365.5
76	210.0
77	157.0
78	104.0
79	77.5
80	51.0
81	38.5
82	26.0
83	24.0
84	22.0
85	14.5
86	7.0
87	6.0
88	5.0
89	2.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4798164.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.788682540840174
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.24471642140512	20.15706821088565
2	10.529245368209056	5.641292231271285
3	4.431525536521877	3.5614419230853303
4	2.393496944355584	2.5647451921925093
5	1.471484517992899	1.97095658081365
6	0.9924729911717152	1.5952226334514288
7	0.7380302195066997	1.3839600079138097
8	0.5361088536604259	1.1489319910434315
9	0.42228355543610435	1.0181178097915573
>10	2.6617860988383084	13.742003047197136
>50	0.31147376811723304	5.844920132938611
>100	0.22131988505715586	11.561803627367063
>500	0.026397859356656367	4.736450363517326
>1k	0.015886857606232353	8.191193073027879
>5k	0.00224662632815407	4.464331985120798
>10k+	0.0015244964369616905	12.41756119038263
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	105512	2.19900778714525	No Hit
AATAAAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAATAAAGGGA	73885	1.5398598297182007	No Hit
AATAAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	43564	0.9079306167942571	No Hit
AATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	41587	0.8667273565472127	No Hit
AATAAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	31964	0.6661714772567173	No Hit
AATAAAGGGAACGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAA	28881	0.6019177335330763	No Hit
AATAAAGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA	28191	0.5875372329916193	Illumina Single End Adapter 2 (97% over 34bp)
AATAAAGGGAACGCAGAGTGCAGTGCTAATAAAGGGAACGCAGAGTGCAG	25667	0.5349337788370718	No Hit
AATAAAGGGGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAAA	23941	0.4989616861782965	No Hit
AATAAAGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA	23124	0.481934339885006	Illumina Single End Adapter 2 (97% over 34bp)
AATAAAGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	22865	0.4765364418556765	No Hit
AATAAAGGGGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAA	20496	0.4271633899966737	No Hit
AATAAAGGGGAGTGCAGTGCTAATAAAGGGGAGTGCAGTGCTAATAAAGG	18588	0.38739817980377494	No Hit
AATAAAGGGACGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAA	16669	0.34740371525441816	No Hit
AATAAAGGGCGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAA	15972	0.3328773255770332	No Hit
AATAAAGGGGCAGAGTGCAGTGCTAATAAAGGGGCAGAGTGCAGTGCTAA	15959	0.3326063886103101	No Hit
AATAAAGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	14144	0.29477941979473815	Illumina Single End Adapter 2 (97% over 34bp)
AATAAAGGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAA	13358	0.2783981539605566	Illumina Single End Adapter 2 (97% over 34bp)
AATAAAGGGAGAGTGCAGTGCTAATAAAGGGAGAGTGCAGTGCTAATAAA	13346	0.27814805829896605	No Hit
AATAAAGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	9847	0.2052243316401857	No Hit
AATAAAGGGGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAAAAA	9780	0.20382796419630508	No Hit
AATAAAGGGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAA	9750	0.20320272504232867	No Hit
AATAAAGGGAACGCAGAGTGCAGTGCTAATAAAGGGGAAAAAAAAAAAAA	9488	0.1977423030976015	No Hit
AATAAAGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	9211	0.19196926157588612	Illumina Single End Adapter 2 (97% over 34bp)
AATAAAGGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	8831	0.1840495656255184	No Hit
AATAAAGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA	8690	0.18111094160182936	Illumina Single End Adapter 2 (100% over 34bp)
AATAAAGGGAACGCAGAGTGCAGTGCTAATAAAGGGGGGAAAAAAAAAAA	8634	0.17994382851440677	No Hit
AATAAAGGAGAGTGCAGTGCTAATAAAGGGGAGTGCAGTGCTAATAAAGG	8402	0.17510864572365595	No Hit
AATAAAGGGGAGTGCAGTGCTAATAAAGGGGAAAAAAAAAAAAAAAAAAA	8042	0.16760577587593922	No Hit
AATAAAGGGGAGTGCAGTGCTAATAAAGGGGGGAAAAAAAAAAAAAAAAA	7993	0.16658455192444444	No Hit
AATAAAGGGAACGCAGAGTGCAGTGCTAATAAAGGGGGTAAAAAAAAAAA	7818	0.1629373235262488	No Hit
AATAAAGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7559	0.15753942549691924	No Hit
AATAAAGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7251	0.1511203035160949	No Hit
AATAAAGGGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAAAA	7195	0.1499531904286723	No Hit
AATAAAGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7185	0.14974477737734684	No Hit
AATAAAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA	7135	0.1487027121207195	Illumina Single End Adapter 2 (100% over 34bp)
AATAAAGGGAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAA	7060	0.1471396142357785	No Hit
AATAAAGGGGCAGAGTGCAGTGCTAATAAAGGGGGGAAAAAAAAAAAAAA	6883	0.1434507032273178	No Hit
AATAAAGGGGCAGAGTGCAGTGCTAATAAAGGGGAAAAAAAAAAAAAAAA	6656	0.1387197269622297	No Hit
AATAAAGGGGAGTGCAGTGCTAATAAAGGGGGTAAAAAAAAAAAAAAAAA	6593	0.1374067247388793	No Hit
AATAAAGGGCGCAGAGTGCAGTGCTAATAAAGGGCGCAGAGTGCAGTGCT	6134	0.12784056568304045	No Hit
AATAAAGGGGCAGAGTGCAGTGCTAATAAAGGGGGTAAAAAAAAAAAAAA	5615	0.11702392831924877	No Hit
AATAAAGGGACGCAGAGTGCAGTGCTAATAAAGGGGAAAAAAAAAAAAAA	5482	0.11425203473662009	No Hit
AATAAAGGGCGCAGAGTGCAGTGCTAATAAAGGGGAAAAAAAAAAAAAAA	5184	0.10804132580712122	No Hit
AATAAAGGGACGCAGAGTGCAGTGCTAATAAAGGGGGGAAAAAAAAAAAA	5123	0.10677000619403589	No Hit
AATAAAGGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAA	5112	0.10654075183757787	Illumina Single End Adapter 2 (100% over 34bp)
AATAAAGGGACGCAGAGTGCAGTGCTAATAAAGGGGGTAAAAAAAAAAAA	5045	0.10514438439369726	No Hit
AATAAAGGGCGCAGAGTGCAGTGCTAATAAAGGGGGTAAAAAAAAAAAAA	4971	0.1036021278138888	No Hit
AATAAAGGGCGCAGAGTGCAGTGCTAATAAAGGGGGGAAAAAAAAAAAAA	4897	0.10205987123408036	No Hit
AATAAAGGGAAACGCAGAGTGCAGTGCTAATAAAGGGAAACGCAGAGTGC	4877	0.10164304513142944	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.40202877600682263	0.0	0.0	0.0	0.0
7	0.4054675913536928	0.0	0.0	0.0	0.0
8	0.41909780491037824	0.0	0.0	0.0	0.0
9	0.47128443296227474	0.0	0.0	0.0	0.0
10	0.7884265731642354	0.0	0.0	0.0	0.0
11	1.148668532380302	0.0	0.0	0.0	0.0
12	1.5369628883047766	0.0	0.0	0.0	0.0
13	1.8796773099043718	0.0	0.0	0.0	0.0
14	2.2807265445699647	0.0	0.0	0.0	0.0
15	2.572963325138532	0.0	0.0	0.0	0.0
16	2.817056690850917	0.0	0.0	0.0	0.0
17	3.0268035857048656	0.0	0.0	0.0	0.0
18	3.21024875348154	0.0	0.0	0.0	0.0
19	3.3973203083512775	0.0	0.0	0.0	0.0
20	3.579494156514867	0.0	0.0	0.0	0.0
21	3.7448490714364913	0.0	0.0	0.0	0.0
22	3.931899785001096	0.0	0.0	0.0	0.0
23	4.154088939019175	0.0	0.0	0.0	0.0
24	4.35570772487143	0.0	0.0	0.0	0.0
25	4.560994580427013	0.0	0.0	0.0	0.0
26	4.779098838639113	0.0	0.0	0.0	0.0
27	5.017773465017036	0.0	0.0	0.0	0.0
28	5.3063838584925405	0.0	0.0	0.0	0.0
29	5.488682754486924	0.0	0.0	0.0	0.0
30	5.6992424602410425	0.0	0.0	0.0	0.0
31	5.835753008859222	0.0	0.0	0.0	0.0
32	5.962759922336961	0.0	0.0	0.0	0.0
33	6.0868490530961425	0.0	0.0	0.0	0.0
34	6.215294016628027	0.0	0.0	0.0	0.0
35	6.425186800617903	0.0	0.0	0.0	0.0
36	6.53008108935001	0.0	0.0	0.0	0.0
37	6.638539241259782	0.0	0.0	0.0	0.0
38	6.749894334582978	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATACTA	20	7.858983E-4	44.000004	9
GGTACGT	20	7.858983E-4	44.000004	9
TAGGTCC	40	8.3218765E-9	44.000004	39
CTATAAC	40	8.3218765E-9	44.000004	42
CGCGCAC	40	8.3218765E-9	44.000004	43
TAATTAC	20	7.858983E-4	44.000004	21
TTCGCCC	20	7.858983E-4	44.000004	19
TATCGTA	20	7.858983E-4	44.000004	35
TGATCGC	25	4.4449596E-5	44.0	18
CTTAACG	25	4.4449596E-5	44.0	19
CCTTACG	25	4.4449596E-5	44.0	44
ATGATTA	25	4.4449596E-5	44.0	17
CTATCTA	30	2.5294048E-6	44.0	17
TCACACG	25	4.4449596E-5	44.0	21
TTAGACT	45	4.802132E-10	44.0	37
ATAGCTA	30	2.5294048E-6	44.0	13
TTACTCA	30	2.5294048E-6	44.0	41
TTATAGA	50	2.7284841E-11	44.0	13
TACGCGC	25	4.4449596E-5	44.0	38
CCATACT	145	0.0	44.0	30
>>END_MODULE