FastQCFastQC Report
Fri 17 Jun 2016
SRR1527083_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527083_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5175148
Sequences flagged as poor quality0
Sequence length52
%GC35

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1016001.9632288777055265No Hit
AATAAAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAATAAAGGGAAG676371.306957791352054No Hit
AATAAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA422640.816672296135299No Hit
AATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA399550.7720552146527984No Hit
AATAAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA311140.6012195206784424No Hit
AATAAAGGGAACGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAA272600.5267482205339828No Hit
AATAAAGGGGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAAAAA236210.45643139094765983No Hit
AATAAAGGGAACGCAGAGTGCAGTGCTAATAAAGGGAACGCAGAGTGCAGTG235450.45496283391315573No Hit
AATAAAGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA232220.4487214665165131Illumina Single End Adapter 2 (97% over 34bp)
AATAAAGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA214550.414577515464292No Hit
AATAAAGGGGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAA188740.36470454564777666No Hit
AATAAAGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA187580.3624630638582703Illumina Single End Adapter 2 (97% over 34bp)
AATAAAGGGGAGTGCAGTGCTAATAAAGGGGAGTGCAGTGCTAATAAAGGGG169140.32683123265267006No Hit
AATAAAGGGACGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAA168530.3256525224012917No Hit
AATAAAGGGCGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAA152030.293769376257452No Hit
AATAAAGGGGCAGAGTGCAGTGCTAATAAAGGGGCAGAGTGCAGTGCTAATA143460.27720946338153035No Hit
AATAAAGGGAGAGTGCAGTGCTAATAAAGGGAGAGTGCAGTGCTAATAAAGG120630.23309478298978117No Hit
AATAAAGGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA117510.22706596990076422Illumina Single End Adapter 2 (97% over 34bp)
AATAAAGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA102840.19871895451105942Illumina Single End Adapter 2 (97% over 34bp)
AATAAAGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA94170.18196581044638724No Hit
AATAAAGGGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAAA89970.1738501005188644No Hit
AATAAAGGGAACGCAGAGTGCAGTGCTAATAAAGGGGAAAAAAAAAAAAAAA88570.17114486387635677No Hit
AATAAAGGGGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAAAAAAA87110.1683236885205988No Hit
AATAAAGGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA85110.16445906474558794No Hit
AATAAAGGGAACGCAGAGTGCAGTGCTAATAAAGGGGGGAAAAAAAAAAAAA82830.16005339364207555No Hit
AATAAAGGGGAGTGCAGTGCTAATAAAGGGGGGAAAAAAAAAAAAAAAAAAA82740.15987948557220005No Hit
AATAAAGGGGAGTGCAGTGCTAATAAAGGGGAAAAAAAAAAAAAAAAAAAAA78410.15151257509930152No Hit
AATAAAGGAGAGTGCAGTGCTAATAAAGGGGAGTGCAGTGCTAATAAAGGGG78190.1510874664840503No Hit
AATAAAGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA73640.14229544739590055Illumina Single End Adapter 2 (100% over 34bp)
AATAAAGGGAACGCAGAGTGCAGTGCTAATAAAGGGGGTAAAAAAAAAAAAA72780.1406336591726459No Hit
AATAAAGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA71590.1383342080265144No Hit
AATAAAGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA71190.13756128327151224No Hit
AATAAAGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70260.13576423321613218No Hit
AATAAAGGGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAAAAAA65570.12670169046373167No Hit
AATAAAGGGGAGTGCAGTGCTAATAAAGGGGGTAAAAAAAAAAAAAAAAAAA63580.12285638980759585No Hit
AATAAAGGGAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAAAA63310.12233466559796936No Hit
AATAAAGGGCAACGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAA61770.11935890529121099No Hit
AATAAAGGGGCAGAGTGCAGTGCTAATAAAGGGGGGAAAAAAAAAAAAAAAA59490.1149532341876986No Hit
AATAAAGGGCGCAGAGTGCAGTGCTAATAAAGGGCGCAGAGTGCAGTGCTAA59190.11437354062144697No Hit
AATAAAGGGGCAGAGTGCAGTGCTAATAAAGGGGAAAAAAAAAAAAAAAAAA58980.11396775512507082No Hit
AATAAAGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA57410.11093402546168728Illumina Single End Adapter 2 (97% over 34bp)
AATAAAGGGAAGCAGAGGTATCAACGCAGAGTGCAGTGCTAATAAAGGGAAG56540.10925291411955755No Hit
AATAAAGGGACGCAGAGTGCAGTGCTAATAAAGGGGAAAAAAAAAAAAAAAA55890.10799691139267902No Hit
AATAAAGGGACGCAGAGTGCAGTGCTAATAAAGGGGGGAAAAAAAAAAAAAA52360.1011758504297848No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATATTA206.3135143E-446.014
CGTGATT253.4188262E-546.018
ACACAAT206.3135143E-446.044
CCTCGAG206.3135143E-446.046
ACTACGA405.6188583E-946.034
TTGCGTA405.6188583E-946.035
ACTAAAT253.4188262E-546.018
GCGTCGC253.4188262E-546.027
CGGTAAT1900.044.7894737
AGAGGTA25400.042.55905514
CGCGCAC650.042.46153643
TTATCCG1900.042.3684233
GAAGCAG307400.041.959669
TACCGAC554.7293724E-1141.8181835
GGAAGCA325150.041.7911728
GGGAAGC338950.041.731827
GAGGTAT25600.041.687515
CTAACCG508.731149E-1041.420
TAACCGG508.731149E-1041.421
AAGCAGT253950.041.16361610
Produced by FastQC (version 0.11.5)