##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527083_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5175148
Sequences flagged as poor quality	0
Sequence length	52
%GC	35
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.701260137874318	33.0	31.0	34.0	28.0	34.0
2	32.01740124147174	33.0	31.0	34.0	30.0	34.0
3	32.091028894246115	34.0	31.0	34.0	30.0	34.0
4	35.69421396257653	37.0	35.0	37.0	35.0	37.0
5	35.94803414317813	37.0	35.0	37.0	35.0	37.0
6	36.17911062640141	37.0	35.0	37.0	35.0	37.0
7	36.332691934607475	37.0	35.0	37.0	35.0	37.0
8	36.4559154636737	37.0	37.0	37.0	35.0	37.0
9	38.47809685829275	39.0	39.0	39.0	37.0	39.0
10	37.64287398157502	39.0	37.0	39.0	35.0	39.0
11	37.236400388935735	39.0	37.0	39.0	34.0	39.0
12	36.18556976534777	38.0	35.0	39.0	31.0	39.0
13	35.80587337792078	38.0	35.0	39.0	31.0	39.0
14	36.688919621235954	39.0	35.0	40.0	31.0	40.0
15	36.950993478833844	40.0	35.0	40.0	32.0	40.0
16	31.981661973725195	38.0	32.0	40.0	6.0	40.0
17	33.76160952305132	38.0	32.0	40.0	17.0	40.0
18	35.741563333067965	38.0	34.0	40.0	27.0	40.0
19	36.228619548658315	38.0	35.0	40.0	30.0	40.0
20	36.777500856014164	39.0	35.0	40.0	31.0	40.0
21	36.99117030083004	39.0	35.0	40.0	32.0	40.0
22	37.1211146038722	40.0	35.0	40.0	32.0	40.0
23	37.15643958395006	40.0	35.0	40.0	32.0	40.0
24	36.81554769061677	39.0	35.0	40.0	32.0	40.0
25	36.384057808588274	38.0	35.0	40.0	31.0	40.0
26	35.80550507927503	37.0	35.0	40.0	30.0	40.0
27	35.60364263978538	37.0	35.0	40.0	30.0	40.0
28	35.4233169756691	36.0	35.0	40.0	30.0	40.0
29	35.19831065700923	36.0	35.0	40.0	30.0	40.0
30	34.87641686769151	35.0	35.0	40.0	26.0	40.0
31	34.6539053569096	35.0	35.0	40.0	25.0	40.0
32	34.23860399741225	35.0	34.0	40.0	23.0	40.0
33	33.80945433831071	35.0	33.0	40.0	21.0	40.0
34	33.568168678460985	35.0	33.0	40.0	20.0	40.0
35	33.35191090187179	35.0	33.0	40.0	19.0	40.0
36	32.91190319581199	35.0	33.0	40.0	16.0	40.0
37	31.943214377637123	35.0	31.0	40.0	12.0	40.0
38	31.316282935289966	35.0	30.0	40.0	10.0	40.0
39	30.234197553383982	35.0	24.0	39.0	9.0	40.0
40	29.53088375443562	35.0	21.0	39.0	8.0	40.0
41	28.867974983517378	35.0	18.0	39.0	8.0	40.0
42	28.42401608611	35.0	15.0	39.0	8.0	40.0
43	27.916718517035648	35.0	15.0	38.0	8.0	40.0
44	27.63078292640133	35.0	12.0	38.0	8.0	40.0
45	27.44982288429239	35.0	10.0	38.0	8.0	40.0
46	27.110343510948866	34.0	10.0	37.0	8.0	40.0
47	26.689336420910088	34.0	10.0	37.0	8.0	40.0
48	26.520288308662863	33.0	10.0	36.0	8.0	40.0
49	26.349986319231835	33.0	10.0	35.0	8.0	40.0
50	25.787768388459614	33.0	10.0	35.0	8.0	40.0
51	25.67519015881285	33.0	10.0	35.0	8.0	40.0
52	25.25009410358892	33.0	9.0	35.0	8.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	4.0
11	15.0
12	25.0
13	41.0
14	167.0
15	586.0
16	2122.0
17	4913.0
18	8974.0
19	14634.0
20	22739.0
21	32627.0
22	45826.0
23	67470.0
24	96331.0
25	133099.0
26	187627.0
27	261273.0
28	329153.0
29	335594.0
30	261762.0
31	196688.0
32	195570.0
33	233312.0
34	324026.0
35	408272.0
36	620099.0
37	643661.0
38	658047.0
39	90491.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	0.0	100.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	100.0	0.0	0.0
6	0.0	100.0	0.0	0.0
7	99.35704254255144	0.538573969285516	0.06146684114154803	0.04291664702149581
8	98.5653357160027	0.9857302631731499	0.27831088115740843	0.17062313966673032
9	96.46381127650841	2.013430340542918	0.754529145833124	0.7682292371155376
10	61.46119879083651	26.100065157556845	4.590458089314548	7.848277962292093
11	46.25177869309245	34.19967892705677	8.937193680258034	10.611348699592746
12	37.84653115234578	38.971368548300454	10.162820464264984	13.019279835088774
13	24.81457535127498	45.609420252329016	12.26326280910227	17.312741587293733
14	21.61279252303509	56.168326007294866	9.208548238620422	13.010333231049623
15	26.41275186719298	53.363420717629715	8.436454377729873	11.78737303744743
16	21.667438303213743	55.89357058001047	14.877101099330877	7.561890017444911
17	28.338184724378895	53.03873435117218	9.349819560715945	9.27326136373298
18	25.411910925059534	51.50967663147025	12.221273671786777	10.857138771683438
19	19.869267506938932	52.10805565367406	18.247323554804616	9.775353284582392
20	20.619854736521546	55.856450868651486	10.48134275580138	13.04235163902559
21	18.088680748840417	50.56487273407446	18.548416393115712	12.7980301239694
22	17.132089748930852	55.49476072954822	11.961706215938172	15.41144330558276
23	17.98107029982524	58.61550239722612	12.178840102737158	11.224587200211472
24	15.450128189570616	55.853243230918224	18.797800565317164	9.89882801419399
25	18.048546630936933	56.26505754038339	12.631194315602182	13.055201513077499
26	19.729580680591162	56.21837288518125	12.946721523712945	11.105324910514637
27	15.565641794205693	55.84534007529833	16.398564833314914	12.190453297181065
28	19.757328679295743	60.143149529250174	12.685376340927835	7.414145450526245
29	24.33426058539775	56.082318805182	11.91268346335216	7.6707371460680935
30	20.547335071383465	57.68977041816002	13.16480224333681	8.598092267119704
31	26.085456879687307	53.84406397652782	11.402282601386473	8.668196542398402
32	22.438334130734038	54.65333551813397	14.84587493922879	8.062455411903196
33	27.129388376912118	52.75910949793127	10.903765457528944	9.20773666762767
34	24.91287205699238	50.83365731762647	11.94690470687988	12.306565918501269
35	25.781059788048573	52.877676155348595	12.088910307492656	9.25235374911017
36	27.689778147407573	50.46640212028719	12.122416595622001	9.721403136683241
37	23.862177468161295	51.242553836141504	14.60758223726162	10.287686458435585
38	25.845656974447877	50.99562370003717	12.844328316794032	10.31439100872091
39	23.595247904021296	49.51773359911639	12.689511488367097	14.197507008495217
40	24.068374469677003	49.4151664841276	15.778157455593542	10.738301590601854
41	23.782720803347075	50.459928875464044	13.408737296015497	12.348613025173387
42	24.76702115572347	50.74898341071599	13.72671854022339	10.757276893337158
43	26.07098386364989	48.071572059388444	15.176358241348847	10.681085835612818
44	25.121407155891966	52.83721354442423	11.953319982346398	10.088059317337397
45	24.836816261100168	52.67397183616778	12.442194889885275	10.047017012846782
46	23.629816963688768	54.15659610121295	12.418079637529207	9.795507297569074
47	26.817745115695242	50.496836032515404	11.924064780369566	10.761354071419793
48	25.36907157051354	51.755060918064565	11.747973198061196	11.1278943133607
49	25.150932881533052	52.52027574863559	11.317608694475984	11.011182675355371
50	21.935894393744874	54.35100600021487	11.722911112880249	11.990188493160002
51	20.46061291387222	54.47836467672036	13.372525771243643	11.688496638163778
52	23.474517057289958	51.44815182097208	13.003106384590355	12.074224737147613
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1452.0
1	1028.5
2	605.0
3	1733.5
4	2862.0
5	28275.0
6	53688.0
7	59472.5
8	65257.0
9	105714.0
10	146171.0
11	113159.5
12	80148.0
13	63735.0
14	48609.5
15	49897.0
16	57775.5
17	65654.0
18	83664.0
19	101674.0
20	122179.0
21	142684.0
22	167384.0
23	192084.0
24	217163.0
25	242242.0
26	256593.5
27	270945.0
28	272143.5
29	273342.0
30	258551.5
31	243761.0
32	247130.0
33	250499.0
34	266470.5
35	282442.0
36	257092.5
37	231743.0
38	217759.5
39	219815.5
40	235855.0
41	275899.0
42	315943.0
43	361915.0
44	407887.0
45	381469.0
46	355051.0
47	340049.5
48	325048.0
49	296732.5
50	268417.0
51	208554.0
52	148691.0
53	107893.0
54	67095.0
55	52869.5
56	38644.0
57	30753.5
58	22863.0
59	18264.0
60	13665.0
61	11874.0
62	10083.0
63	8007.0
64	5058.5
65	4186.0
66	3406.5
67	2627.0
68	2120.0
69	1613.0
70	1379.5
71	1146.0
72	1331.0
73	1516.0
74	920.0
75	324.0
76	239.0
77	154.0
78	116.5
79	79.0
80	57.5
81	36.0
82	31.0
83	26.0
84	20.5
85	15.0
86	9.0
87	3.0
88	2.5
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	5175148.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.68378509553435
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.1279026502254	24.753876775345702
2	9.514796172742795	6.02929514329991
3	3.9444073845517242	3.749212677041267
4	2.158516419586716	2.735598814534711
5	1.2744001718216769	2.018891058485503
6	0.906448848488847	1.7231838309369123
7	0.6039346590082524	1.33944551634336
8	0.4515724117237305	1.1446018598501468
9	0.34528763288705633	0.9846017240885327
>10	2.2340894338899147	13.473610454872839
>50	0.23564359080172578	5.244735740847533
>100	0.16840411685479442	10.28575380367224
>500	0.018841592340726916	4.004751350590175
>1k	0.012732543612908152	7.637932866918934
>5k	0.0018005617230375164	3.9391250697125693
>10k+	0.0012218097406326003	10.935383313459782
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	101600	1.9632288777055265	No Hit
AATAAAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAATAAAGGGAAG	67637	1.306957791352054	No Hit
AATAAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	42264	0.816672296135299	No Hit
AATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	39955	0.7720552146527984	No Hit
AATAAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	31114	0.6012195206784424	No Hit
AATAAAGGGAACGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAA	27260	0.5267482205339828	No Hit
AATAAAGGGGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAAAAA	23621	0.45643139094765983	No Hit
AATAAAGGGAACGCAGAGTGCAGTGCTAATAAAGGGAACGCAGAGTGCAGTG	23545	0.45496283391315573	No Hit
AATAAAGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	23222	0.4487214665165131	Illumina Single End Adapter 2 (97% over 34bp)
AATAAAGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	21455	0.414577515464292	No Hit
AATAAAGGGGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAA	18874	0.36470454564777666	No Hit
AATAAAGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	18758	0.3624630638582703	Illumina Single End Adapter 2 (97% over 34bp)
AATAAAGGGGAGTGCAGTGCTAATAAAGGGGAGTGCAGTGCTAATAAAGGGG	16914	0.32683123265267006	No Hit
AATAAAGGGACGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAA	16853	0.3256525224012917	No Hit
AATAAAGGGCGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAA	15203	0.293769376257452	No Hit
AATAAAGGGGCAGAGTGCAGTGCTAATAAAGGGGCAGAGTGCAGTGCTAATA	14346	0.27720946338153035	No Hit
AATAAAGGGAGAGTGCAGTGCTAATAAAGGGAGAGTGCAGTGCTAATAAAGG	12063	0.23309478298978117	No Hit
AATAAAGGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA	11751	0.22706596990076422	Illumina Single End Adapter 2 (97% over 34bp)
AATAAAGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA	10284	0.19871895451105942	Illumina Single End Adapter 2 (97% over 34bp)
AATAAAGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	9417	0.18196581044638724	No Hit
AATAAAGGGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAAA	8997	0.1738501005188644	No Hit
AATAAAGGGAACGCAGAGTGCAGTGCTAATAAAGGGGAAAAAAAAAAAAAAA	8857	0.17114486387635677	No Hit
AATAAAGGGGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAAAAAAA	8711	0.1683236885205988	No Hit
AATAAAGGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	8511	0.16445906474558794	No Hit
AATAAAGGGAACGCAGAGTGCAGTGCTAATAAAGGGGGGAAAAAAAAAAAAA	8283	0.16005339364207555	No Hit
AATAAAGGGGAGTGCAGTGCTAATAAAGGGGGGAAAAAAAAAAAAAAAAAAA	8274	0.15987948557220005	No Hit
AATAAAGGGGAGTGCAGTGCTAATAAAGGGGAAAAAAAAAAAAAAAAAAAAA	7841	0.15151257509930152	No Hit
AATAAAGGAGAGTGCAGTGCTAATAAAGGGGAGTGCAGTGCTAATAAAGGGG	7819	0.1510874664840503	No Hit
AATAAAGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	7364	0.14229544739590055	Illumina Single End Adapter 2 (100% over 34bp)
AATAAAGGGAACGCAGAGTGCAGTGCTAATAAAGGGGGTAAAAAAAAAAAAA	7278	0.1406336591726459	No Hit
AATAAAGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7159	0.1383342080265144	No Hit
AATAAAGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7119	0.13756128327151224	No Hit
AATAAAGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7026	0.13576423321613218	No Hit
AATAAAGGGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAAAAAA	6557	0.12670169046373167	No Hit
AATAAAGGGGAGTGCAGTGCTAATAAAGGGGGTAAAAAAAAAAAAAAAAAAA	6358	0.12285638980759585	No Hit
AATAAAGGGAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAAAA	6331	0.12233466559796936	No Hit
AATAAAGGGCAACGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAA	6177	0.11935890529121099	No Hit
AATAAAGGGGCAGAGTGCAGTGCTAATAAAGGGGGGAAAAAAAAAAAAAAAA	5949	0.1149532341876986	No Hit
AATAAAGGGCGCAGAGTGCAGTGCTAATAAAGGGCGCAGAGTGCAGTGCTAA	5919	0.11437354062144697	No Hit
AATAAAGGGGCAGAGTGCAGTGCTAATAAAGGGGAAAAAAAAAAAAAAAAAA	5898	0.11396775512507082	No Hit
AATAAAGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA	5741	0.11093402546168728	Illumina Single End Adapter 2 (97% over 34bp)
AATAAAGGGAAGCAGAGGTATCAACGCAGAGTGCAGTGCTAATAAAGGGAAG	5654	0.10925291411955755	No Hit
AATAAAGGGACGCAGAGTGCAGTGCTAATAAAGGGGAAAAAAAAAAAAAAAA	5589	0.10799691139267902	No Hit
AATAAAGGGACGCAGAGTGCAGTGCTAATAAAGGGGGGAAAAAAAAAAAAAA	5236	0.1011758504297848	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.37309077923955025	0.0	0.0	0.0	0.0
7	0.37562210781218236	0.0	0.0	0.0	0.0
8	0.3877570264657165	0.0	0.0	0.0	0.0
9	0.43722421078585577	0.0	0.0	0.0	0.0
10	0.7149167521392625	0.0	0.0	0.0	0.0
11	1.0193138437779943	0.0	0.0	0.0	0.0
12	1.3431113467672808	0.0	0.0	0.0	0.0
13	1.6271225479928304	0.0	0.0	0.0	0.0
14	2.0094497780546567	0.0	0.0	0.0	0.0
15	2.2642444235411237	0.0	0.0	0.0	0.0
16	2.5128170247498236	0.0	0.0	0.0	0.0
17	2.7219897865722875	0.0	0.0	0.0	0.0
18	2.9043034131584258	0.0	0.0	0.0	0.0
19	3.0850325439968094	0.0	0.0	0.0	1.9323118875054394E-5
20	3.26116277254293	0.0	0.0	0.0	1.9323118875054394E-5
21	3.417738004787496	0.0	0.0	0.0	1.9323118875054394E-5
22	3.5960710688853728	0.0	0.0	0.0	1.9323118875054394E-5
23	3.811040766370353	0.0	0.0	0.0	1.9323118875054394E-5
24	3.9944558107323695	0.0	0.0	0.0	1.9323118875054394E-5
25	4.180846615401144	0.0	0.0	0.0	1.9323118875054394E-5
26	4.3802998484294555	0.0	0.0	0.0	1.9323118875054394E-5
27	4.596216378739314	0.0	0.0	0.0	1.9323118875054394E-5
28	4.845774459010641	0.0	0.0	0.0	1.9323118875054394E-5
29	4.9998570089203245	0.0	0.0	0.0	1.9323118875054394E-5
30	5.184102947393968	0.0	0.0	0.0	1.9323118875054394E-5
31	5.298070702519039	0.0	0.0	0.0	1.9323118875054394E-5
32	5.40664730747797	0.0	0.0	0.0	1.9323118875054394E-5
33	5.514972711891525	0.0	0.0	0.0	1.9323118875054394E-5
34	5.62704680136684	0.0	0.0	0.0	1.9323118875054394E-5
35	5.830654504953288	0.0	0.0	0.0	1.9323118875054394E-5
36	5.923134951889299	0.0	0.0	0.0	1.9323118875054394E-5
37	6.018320635467816	0.0	0.0	0.0	1.9323118875054394E-5
38	6.119052054163475	0.0	0.0	0.0	1.9323118875054394E-5
39	6.227821890311156	0.0	0.0	0.0	1.9323118875054394E-5
40	6.341750999198477	0.0	0.0	0.0	3.864623775010879E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATATTA	20	6.3135143E-4	46.0	14
CGTGATT	25	3.4188262E-5	46.0	18
ACACAAT	20	6.3135143E-4	46.0	44
CCTCGAG	20	6.3135143E-4	46.0	46
ACTACGA	40	5.6188583E-9	46.0	34
TTGCGTA	40	5.6188583E-9	46.0	35
ACTAAAT	25	3.4188262E-5	46.0	18
GCGTCGC	25	3.4188262E-5	46.0	27
CGGTAAT	190	0.0	44.78947	37
AGAGGTA	2540	0.0	42.559055	14
CGCGCAC	65	0.0	42.461536	43
TTATCCG	190	0.0	42.36842	33
GAAGCAG	30740	0.0	41.95966	9
TACCGAC	55	4.7293724E-11	41.81818	35
GGAAGCA	32515	0.0	41.791172	8
GGGAAGC	33895	0.0	41.73182	7
GAGGTAT	2560	0.0	41.6875	15
CTAACCG	50	8.731149E-10	41.4	20
TAACCGG	50	8.731149E-10	41.4	21
AAGCAGT	25395	0.0	41.163616	10
>>END_MODULE