FastQCFastQC Report
Fri 17 Jun 2016
SRR1527082_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527082_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5158573
Sequences flagged as poor quality0
Sequence length52
%GC35

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA933581.8097640568428517No Hit
AATAAAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAATAAAGGGAAG659071.2776207683791623No Hit
AATAAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA387930.7520102943197663No Hit
AATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA368050.7134725048962184No Hit
AATAAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA281230.5451701468603817No Hit
AATAAAGGGAACGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAA265850.5153557001131902No Hit
AATAAAGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA240740.46667944797912136Illumina Single End Adapter 2 (97% over 34bp)
AATAAAGGGAACGCAGAGTGCAGTGCTAATAAAGGGAACGCAGAGTGCAGTG224740.4356631184631874No Hit
AATAAAGGGGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAAAAA216000.4187204484651085No Hit
AATAAAGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA195010.3780309011813926No Hit
AATAAAGGGGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAA189820.3679699792946615No Hit
AATAAAGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA179650.348255224846096Illumina Single End Adapter 2 (97% over 34bp)
AATAAAGGGACGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAA156480.30333970266583415No Hit
AATAAAGGGCGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAA153060.29670996223180324No Hit
AATAAAGGGGAGTGCAGTGCTAATAAAGGGGAGTGCAGTGCTAATAAAGGGG152440.2955080794630608No Hit
AATAAAGGGGCAGAGTGCAGTGCTAATAAAGGGGCAGAGTGCAGTGCTAATA139200.26984206678862543No Hit
AATAAAGGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA121250.23504562211293703Illumina Single End Adapter 2 (97% over 34bp)
AATAAAGGGAGAGTGCAGTGCTAATAAAGGGAGAGTGCAGTGCTAATAAAGG120360.23332033878361325No Hit
AATAAAGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA98950.1918166128501041Illumina Single End Adapter 2 (97% over 34bp)
AATAAAGGGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAAA90820.17605644041482016No Hit
AATAAAGGGAACGCAGAGTGCAGTGCTAATAAAGGGGAAAAAAAAAAAAAAA87360.1693491591569994No Hit
AATAAAGGGGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAAAAAAA86560.16779834268120272No Hit
AATAAAGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA86040.16679031197193486No Hit
AATAAAGGGAACGCAGAGTGCAGTGCTAATAAAGGGGGGAAAAAAAAAAAAA77280.14980887156196104No Hit
AATAAAGGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA75560.14647461613899812No Hit
AATAAAGGGGAGTGCAGTGCTAATAAAGGGGGGAAAAAAAAAAAAAAAAAAA74560.14453609554425226No Hit
AATAAAGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA74500.1444197843085675Illumina Single End Adapter 2 (100% over 34bp)
AATAAAGGGGAGTGCAGTGCTAATAAAGGGGAAAAAAAAAAAAAAAAAAAAA72130.1398254904990198No Hit
AATAAAGGAGAGTGCAGTGCTAATAAAGGGGAGTGCAGTGCTAATAAAGGGG70410.13649123507605687No Hit
AATAAAGGGAACGCAGAGTGCAGTGCTAATAAAGGGGGTAAAAAAAAAAAAA68780.13333144650662113No Hit
AATAAAGGGCAACGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAA65990.12792297404728015No Hit
AATAAAGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA65540.12705063977964448No Hit
AATAAAGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA65250.1264884688071682No Hit
AATAAAGGGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAAAAAA64140.12433671094700027No Hit
AATAAAGGGAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAAAA63420.12294097611878323No Hit
AATAAAGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA62920.1219717158214103No Hit
AATAAAGGGGCAGAGTGCAGTGCTAATAAAGGGGGGAAAAAAAAAAAAAAAA61390.11900577931144912No Hit
AATAAAGGGGCAGAGTGCAGTGCTAATAAAGGGGAAAAAAAAAAAAAAAAAA59730.11578783512417097No Hit
AATAAAGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA59170.11470226359111327Illumina Single End Adapter 2 (97% over 34bp)
AATAAAGGGCGCAGAGTGCAGTGCTAATAAAGGGCGCAGAGTGCAGTGCTAA57830.1121046459941538No Hit
AATAAAGGGGAGTGCAGTGCTAATAAAGGGGGTAAAAAAAAAAAAAAAAAAA57710.1118720235227843No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGTT301.8626761E-646.019
ACGTTAC206.3135126E-446.025
TAGGTTG453.110472E-1046.032
TTATGCG206.3135126E-446.043
ATCGTAC405.6188583E-946.036
ACTAGTC206.3135126E-446.018
ACTACAT206.3135126E-446.034
TCCACGT301.8626761E-646.020
TTGCGTA301.8626761E-646.035
TTGATGC351.0205076E-745.99999637
CCTTACG351.0205076E-745.99999644
ACGGATT351.0205076E-745.99999614
CGTCTAC2950.044.4406835
TGATTGA6550.043.89312738
GTCTACT3000.043.69999736
CCATACT1800.042.16666830
CCGGACT1150.042.041
CTGATTG8900.041.86516637
GGAAGCA312250.041.8530048
GTTCGGC554.7293724E-1141.8181832
Produced by FastQC (version 0.11.5)