FastQCFastQC Report
Fri 17 Jun 2016
SRR1527078_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527078_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2191749
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGAAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC144690.6601577096647472No Hit
CTGAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC98360.4487740156377395No Hit
CTGAAAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC87650.3999089311777945No Hit
CTGAAAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT75560.3447475053028426No Hit
CTGAAAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG60560.27630901166146304No Hit
CTGAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG54950.2507130150395871No Hit
CTGAAAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA50960.23250837573098015No Hit
CTGAAAGGGGTTACCCTGTCCCTGCCTCTTTCTCCTCTTTGCCCACTTCT47720.21772566110444214No Hit
CTGAAAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTC46370.21156619667671803No Hit
CTGAAAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC44640.20367295707674557No Hit
CTGAAAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCAT40840.18633520535426273No Hit
CTGAAAGGGGCTTTTTCGCAACGGGTTTGCCGTCAGAACGCAGGTGTTGT39960.18232014706063515No Hit
CTGAAAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTC39280.17921760201555925No Hit
CTGAAAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC37290.17013809519246956No Hit
CTGAAAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTT35080.160054823795973No Hit
CTGAAAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGT35030.15982669548383507No Hit
CTGAAAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG34640.15804729464915918No Hit
CTGAAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT34090.15553788321564194No Hit
CTGAAAGGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAAC33110.15106656829773848No Hit
CTGAAAGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAA31850.14531773483186258No Hit
CTGAAAGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCT31790.14504398085729708No Hit
CTGAAAGGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTCACGTCTCGTG31490.14367521098446948No Hit
CTGAAAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTA31320.14289957472320053No Hit
CTGAAAGGGGCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGGT29570.1349150837983729No Hit
CTGAAAGGGGGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC28080.12811686009666254No Hit
CTGAAAGGGGCTCTTGAGTCACCCCCGCGCAGCCTAGGCTTGCCGTGCGA27570.1257899513128556No Hit
CTGAAAGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGA26660.12163801603194525No Hit
CTGAAAGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAA26600.12136426205737974No Hit
CTGAAAGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAA25990.11858109664929697No Hit
CTGAAAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT25900.11817046568744871No Hit
CTGAAAGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAG25780.11762295773831766No Hit
CTGAAAGGGGACTGTCGAGTCGCGTCCACCCGCGAGCACAGCTTCTTTGC25320.1155241772666487No Hit
CTGAAAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATC24960.11388165341925559No Hit
CTGAAAGGGGACTACCCAGATGCTTACACCACATGAAACACTGTCTCTTC24280.1107791083741797No Hit
CTGAAAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG24270.11073348271175211No Hit
CTGAAAGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCG23700.1081328199533797No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTAA207.858046E-444.018
CGTTGAC408.3182385E-944.018
TTCGTAA750.044.021
ATCGTAA254.444165E-544.010
TAACACG302.5287736E-644.033
TATTCGA302.5287736E-644.027
AACGAAT254.444165E-544.027
ACGTAGT454.802132E-1044.041
TAGTCGA254.444165E-544.040
GCGTAAT254.444165E-544.021
ATCGAAT207.858046E-444.016
TGAAAGG2224600.043.2108232
CTGAAAG2228650.043.184621
GAAAGGG2222500.043.1754343
AAAGGGA489150.043.172444
AAAGGGG1590500.042.9999354
AAGGGGG643950.042.978495
AGGGGGC172900.042.829386
AAGGGGC339350.042.7423025
AAGGGGT176050.042.7003675
Produced by FastQC (version 0.11.5)