##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1527078_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2191749 Sequences flagged as poor quality 0 Sequence length 50 %GC 55 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.350947120313506 33.0 31.0 34.0 31.0 34.0 2 32.76626657523284 34.0 31.0 34.0 31.0 34.0 3 33.071955319701296 34.0 31.0 34.0 31.0 34.0 4 36.118325592939705 37.0 35.0 37.0 35.0 37.0 5 36.26883050933296 37.0 37.0 37.0 35.0 37.0 6 36.37880888733153 37.0 37.0 37.0 35.0 37.0 7 36.456087809324885 37.0 37.0 37.0 35.0 37.0 8 36.62193982978891 37.0 37.0 37.0 35.0 37.0 9 38.753794344151636 39.0 39.0 39.0 39.0 39.0 10 38.297675965632926 39.0 39.0 39.0 37.0 39.0 11 37.95227008202125 39.0 38.0 39.0 35.0 39.0 12 37.364496573284626 39.0 37.0 39.0 35.0 39.0 13 37.19666668035437 39.0 37.0 39.0 34.0 39.0 14 37.883504452380265 40.0 38.0 40.0 33.0 40.0 15 37.97894923186916 40.0 38.0 40.0 34.0 40.0 16 37.90538423879742 40.0 38.0 40.0 34.0 40.0 17 37.95176500593818 40.0 38.0 40.0 34.0 40.0 18 37.937911686055294 40.0 37.0 40.0 34.0 40.0 19 37.917617847664125 40.0 37.0 40.0 34.0 40.0 20 37.66222774596908 40.0 37.0 40.0 33.0 40.0 21 37.806778969672166 40.0 37.0 40.0 33.0 40.0 22 37.68306270471665 40.0 37.0 40.0 33.0 40.0 23 37.756272273878075 40.0 37.0 40.0 34.0 40.0 24 37.749768791955645 40.0 37.0 40.0 34.0 40.0 25 37.73370673375464 40.0 37.0 40.0 34.0 40.0 26 37.565467122375786 40.0 36.0 40.0 33.0 40.0 27 37.483690878836946 40.0 36.0 40.0 33.0 40.0 28 37.31630948616835 40.0 36.0 40.0 33.0 40.0 29 37.558235911137636 40.0 36.0 40.0 33.0 40.0 30 37.60208856032329 40.0 37.0 40.0 34.0 40.0 31 37.539189022100615 40.0 36.0 40.0 33.0 40.0 32 36.81798645739088 40.0 35.0 40.0 32.0 40.0 33 37.01721501869055 40.0 35.0 40.0 32.0 40.0 34 36.96435495122845 40.0 35.0 40.0 32.0 40.0 35 36.93159344432232 40.0 35.0 40.0 32.0 40.0 36 36.74020109054458 39.0 35.0 40.0 31.0 40.0 37 36.67435641581221 39.0 35.0 40.0 31.0 40.0 38 36.43878473310584 39.0 35.0 40.0 31.0 40.0 39 36.37753866888955 39.0 35.0 40.0 31.0 40.0 40 36.276443151109 39.0 35.0 40.0 31.0 40.0 41 36.077551763454665 39.0 35.0 40.0 30.0 40.0 42 35.96691226960751 38.0 35.0 40.0 30.0 40.0 43 35.90591030268521 38.0 35.0 40.0 30.0 40.0 44 35.721534263275586 38.0 35.0 40.0 30.0 40.0 45 35.61082176836855 38.0 35.0 40.0 29.0 40.0 46 35.355305283588585 37.0 35.0 40.0 29.0 40.0 47 35.20980823990338 37.0 34.0 40.0 28.0 40.0 48 35.07039629081615 37.0 34.0 40.0 28.0 40.0 49 34.74877506502798 36.0 34.0 40.0 27.0 40.0 50 34.36631201839262 35.0 33.0 40.0 26.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 1.0 13 3.0 14 6.0 15 17.0 16 75.0 17 194.0 18 398.0 19 700.0 20 1142.0 21 1887.0 22 3002.0 23 4632.0 24 6826.0 25 9062.0 26 12106.0 27 15671.0 28 20472.0 29 27456.0 30 36870.0 31 47705.0 32 57548.0 33 77437.0 34 143565.0 35 170711.0 36 186058.0 37 335351.0 38 660540.0 39 372313.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 0.0 0.0 100.0 0.0 3 100.0 0.0 0.0 0.0 4 0.0 100.0 0.0 0.0 5 0.0 100.0 0.0 0.0 6 0.0 100.0 0.0 0.0 7 99.7990645826689 0.14417709327117292 0.03371736453398633 0.023040959525931114 8 99.82931439685841 0.1382457571555867 0.022082820614951804 0.010357025371062105 9 99.73756118971653 0.1714612394028696 0.04293374834435877 0.04804382253624845 10 71.1012073006535 22.098835222463887 1.8917768412350138 4.908180635647604 11 37.37129570949958 25.799350199315707 17.31959270883664 19.50976138234807 12 31.560365717059753 14.118039976292906 28.693979100709065 25.62761520593827 13 25.349526793442134 16.41871400420395 30.162258543291227 28.069500659062697 14 23.9297474300205 17.40281391710456 32.39709474031926 26.27034391255568 15 24.52009787617104 18.2564700611247 30.215366814356937 27.008065248347325 16 30.241054062303668 18.2233914558647 26.183518276955986 25.35203620487565 17 31.571178999055093 18.172564467920367 23.925914874376584 26.330341658647953 18 29.74104242775975 19.045657144134662 24.63261075971747 26.580689668388125 19 30.416393483012882 18.57954537677444 23.01084658872891 27.99321455148377 20 29.003229840643247 20.4011043235334 24.162392682738762 26.433273153084592 21 29.047669235847717 18.681473106637668 26.213083706209062 26.057773951305556 22 29.34742983799696 18.454120430761005 26.072739168581805 26.12571056266023 23 29.114145826004712 19.942612041798583 25.210642277012557 25.732599855184148 24 28.63106131222143 20.720118955227083 23.198641815280855 27.450177917270636 25 28.397822925891603 20.75319756048708 22.68872941199015 28.160250101631163 26 27.765724998619824 20.405165007489455 23.37913693584439 28.449973058046336 27 26.854169888979072 17.44401389027667 25.026223349480254 30.675592871264 28 27.447668505837118 19.436440942826938 23.458206208831395 29.65768434250455 29 29.22419492378005 20.13455920363144 22.829484580579255 27.81176129200926 30 28.402750497433782 18.019581621800672 24.970514415656172 28.60715346510937 31 29.931438317070064 20.666965058498942 22.236670348657626 27.164926275773364 32 31.86537327038817 20.10973884327083 22.006443256048026 26.018444630292976 33 32.73226085651231 18.80151422448465 23.003683359727777 25.462541559275266 34 29.447350038713378 20.990542256435386 25.844747733431156 23.717359971420084 35 28.311271044266473 23.71859186430563 25.811577876846297 22.158559214581597 36 31.050635816418758 22.99159255918447 22.4872008610475 23.470570763349272 37 29.795154463398866 23.59645196598698 21.520849330831222 25.087544239782932 38 30.864939370338483 23.15789809873302 22.801151044211725 23.17601148671677 39 30.742069461420996 21.375440344674505 22.106819713388713 25.77567048051579 40 29.50155332567735 22.668973500159005 22.45225160362797 25.37722157053568 41 27.416916809360924 21.21178109354675 23.19877869226814 28.172523404824183 42 26.266602608236617 20.437787356125174 23.81686954117465 29.47874049446356 43 26.670435346383186 19.348794045303546 26.899384920444813 27.081385687868455 44 26.548112945414825 18.811323741906577 25.730364197725198 28.9101991149534 45 26.914806394345337 19.84752816129949 25.401859428246574 27.835806016108595 46 28.112571284394335 21.050996259151937 23.71827248466864 27.11815997178509 47 25.68172724157739 20.333988974102418 26.858914957871544 27.12536882644865 48 28.42506144636087 21.48371004161517 25.259416110147647 24.83181240187631 49 29.041144766120574 21.323837720468905 24.115124496463782 25.51989301694674 50 25.28533148640652 21.006283109972905 25.342044184804006 28.366341218816572 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 31.5 4 63.0 5 34.5 6 6.0 7 14.5 8 23.0 9 106.0 10 189.0 11 1022.5 12 1856.0 13 2501.0 14 3146.0 15 2977.0 16 2808.0 17 3625.0 18 4442.0 19 5186.5 20 5931.0 21 6485.0 22 7039.0 23 7708.0 24 8377.0 25 8401.5 26 8426.0 27 10487.5 28 12549.0 29 14007.5 30 15466.0 31 16696.5 32 17927.0 33 26400.5 34 34874.0 35 32571.5 36 30269.0 37 33444.0 38 36619.0 39 39172.5 40 41726.0 41 44834.0 42 47942.0 43 50788.0 44 53634.0 45 64655.5 46 75677.0 47 82553.0 48 89429.0 49 92592.0 50 95755.0 51 102765.0 52 109775.0 53 125370.0 54 140965.0 55 159999.0 56 179033.0 57 198838.0 58 218643.0 59 210711.0 60 202779.0 61 200573.5 62 198368.0 63 182981.5 64 167595.0 65 143914.5 66 120234.0 67 100141.5 68 80049.0 69 74819.0 70 69589.0 71 57530.0 72 45471.0 73 41250.5 74 37030.0 75 26792.0 76 16554.0 77 11576.5 78 6599.0 79 4694.5 80 2790.0 81 2025.0 82 1260.0 83 932.5 84 605.0 85 413.5 86 222.0 87 117.5 88 13.0 89 7.0 90 1.0 91 1.0 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 2191749.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 7.131718556411368 #Duplication Level Percentage of deduplicated Percentage of total 1 65.3053672048103 4.657394991278039 2 10.967095148837995 1.5642847196579404 3 4.943465747668571 1.0576621921689584 4 2.718036485464502 0.775370849615613 5 1.5581308142959083 0.5556075220815241 6 0.979819122295009 0.4192676529838809 7 0.6848560249955388 0.34189402953515674 8 0.48177337677259896 0.27486977048912875 9 0.3615900483720286 0.23208826120096324 >10 4.876590860449083 9.482569985190915 >50 3.646438477853024 18.90463468152224 >100 3.233117580985963 40.89662762149847 >500 0.16158910669116128 7.736848460676186 >1k 0.07745593543873848 10.373529462825383 >5k 0.004006341488210611 2.0383336093480855 >10k+ 6.67723581368435E-4 0.6890161899275173 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGAAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC 14469 0.6601577096647472 No Hit CTGAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC 9836 0.4487740156377395 No Hit CTGAAAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC 8765 0.3999089311777945 No Hit CTGAAAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT 7556 0.3447475053028426 No Hit CTGAAAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG 6056 0.27630901166146304 No Hit CTGAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG 5495 0.2507130150395871 No Hit CTGAAAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA 5096 0.23250837573098015 No Hit CTGAAAGGGGTTACCCTGTCCCTGCCTCTTTCTCCTCTTTGCCCACTTCT 4772 0.21772566110444214 No Hit CTGAAAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTC 4637 0.21156619667671803 No Hit CTGAAAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC 4464 0.20367295707674557 No Hit CTGAAAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCAT 4084 0.18633520535426273 No Hit CTGAAAGGGGCTTTTTCGCAACGGGTTTGCCGTCAGAACGCAGGTGTTGT 3996 0.18232014706063515 No Hit CTGAAAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTC 3928 0.17921760201555925 No Hit CTGAAAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC 3729 0.17013809519246956 No Hit CTGAAAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTT 3508 0.160054823795973 No Hit CTGAAAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGT 3503 0.15982669548383507 No Hit CTGAAAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG 3464 0.15804729464915918 No Hit CTGAAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT 3409 0.15553788321564194 No Hit CTGAAAGGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAAC 3311 0.15106656829773848 No Hit CTGAAAGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAA 3185 0.14531773483186258 No Hit CTGAAAGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCT 3179 0.14504398085729708 No Hit CTGAAAGGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTCACGTCTCGTG 3149 0.14367521098446948 No Hit CTGAAAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTA 3132 0.14289957472320053 No Hit CTGAAAGGGGCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGGT 2957 0.1349150837983729 No Hit CTGAAAGGGGGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC 2808 0.12811686009666254 No Hit CTGAAAGGGGCTCTTGAGTCACCCCCGCGCAGCCTAGGCTTGCCGTGCGA 2757 0.1257899513128556 No Hit CTGAAAGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGA 2666 0.12163801603194525 No Hit CTGAAAGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAA 2660 0.12136426205737974 No Hit CTGAAAGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAA 2599 0.11858109664929697 No Hit CTGAAAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT 2590 0.11817046568744871 No Hit CTGAAAGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAG 2578 0.11762295773831766 No Hit CTGAAAGGGGACTGTCGAGTCGCGTCCACCCGCGAGCACAGCTTCTTTGC 2532 0.1155241772666487 No Hit CTGAAAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATC 2496 0.11388165341925559 No Hit CTGAAAGGGGACTACCCAGATGCTTACACCACATGAAACACTGTCTCTTC 2428 0.1107791083741797 No Hit CTGAAAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG 2427 0.11073348271175211 No Hit CTGAAAGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCG 2370 0.1081328199533797 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.00917075814794486 0.0 0.0 0.0 0.0 7 0.009216383810372446 0.0 0.0 0.0 0.0 8 0.009262009472800033 0.0 0.0 0.0 0.0 9 0.009855143084358656 0.0 0.0 0.0 0.0 10 0.014600211976827638 0.0 0.0 0.0 0.0 11 0.024500980723613883 0.0 0.0 0.0 0.0 12 0.028744167329379414 0.0 0.0 0.0 0.0 13 0.033899867183696676 0.0 0.0 0.0 0.0 14 0.04594504206457947 0.0 0.0 0.0 0.0 15 0.05470516925067606 0.0 0.0 0.0 0.0 16 0.06921412990264852 0.0 0.0 0.0 0.0 17 0.07660548721591752 0.0 0.0 0.0 0.0 18 0.08249119766907616 0.0 0.0 0.0 0.0 19 0.09252884340314516 0.0 0.0 0.0 0.0 20 0.09946394409213828 0.0 0.0 0.0 0.0 21 0.1048021465961659 0.0 0.0 0.0 0.0 22 0.11264976053371074 0.0 0.0 0.0 0.0 23 0.11807921436259353 0.0 0.0 0.0 0.0 24 0.12378242216604182 0.0 0.0 0.0 0.0 25 0.12779748045966943 0.0 0.0 0.0 0.0 26 0.13277067766427633 0.0 0.0 0.0 0.0 27 0.13788075185616602 0.0 0.0 0.0 0.0 28 0.14376646230932466 0.0 0.0 0.0 0.0 29 0.14873965951393156 0.0 0.0 0.0 0.0 30 0.1539409850306764 0.0 0.0 0.0 0.0 31 0.15950731584684194 0.0 0.0 0.0 0.0 32 0.16762868375895232 0.0 0.0 0.0 0.0 33 0.1720087473520006 0.0 0.0 0.0 0.0 34 0.1773925755184558 0.0 0.0 0.0 0.0 35 0.18569644608027652 0.0 0.0 0.0 0.0 36 0.1942284449542352 0.0 0.0 0.0 0.0 37 0.20212168455420762 0.0 0.0 0.0 0.0 38 0.20709488175881455 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGTTAA 20 7.858046E-4 44.0 18 CGTTGAC 40 8.3182385E-9 44.0 18 TTCGTAA 75 0.0 44.0 21 ATCGTAA 25 4.444165E-5 44.0 10 TAACACG 30 2.5287736E-6 44.0 33 TATTCGA 30 2.5287736E-6 44.0 27 AACGAAT 25 4.444165E-5 44.0 27 ACGTAGT 45 4.802132E-10 44.0 41 TAGTCGA 25 4.444165E-5 44.0 40 GCGTAAT 25 4.444165E-5 44.0 21 ATCGAAT 20 7.858046E-4 44.0 16 TGAAAGG 222460 0.0 43.210823 2 CTGAAAG 222865 0.0 43.18462 1 GAAAGGG 222250 0.0 43.175434 3 AAAGGGA 48915 0.0 43.17244 4 AAAGGGG 159050 0.0 42.999935 4 AAGGGGG 64395 0.0 42.97849 5 AGGGGGC 17290 0.0 42.82938 6 AAGGGGC 33935 0.0 42.742302 5 AAGGGGT 17605 0.0 42.700367 5 >>END_MODULE