FastQCFastQC Report
Fri 17 Jun 2016
SRR1527077_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527077_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2346676
Sequences flagged as poor quality0
Sequence length52
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGAAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA147060.6266736439116435No Hit
CTGAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC94290.40180237919508277No Hit
CTGAAAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT79630.33933103675155835No Hit
CTGAAAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG72700.3097999042049264No Hit
CTGAAAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG59130.25197342965113206No Hit
CTGAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT51910.2212065065650307No Hit
CTGAAAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC50330.21447357879826615No Hit
CTGAAAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA46400.19772648631511125No Hit
CTGAAAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG41980.17889133395492177No Hit
CTGAAAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA38840.16551070535514917No Hit
CTGAAAGGGGCTTTTTCGCAACGGGTTTGCCGTCAGAACGCAGGTGTTGTGA37340.15911868532341064No Hit
CTGAAAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC37170.15839425638648028No Hit
CTGAAAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT36230.15438859049992415No Hit
CTGAAAGGGGTTACCCTGTCCCTGCCTCTTTCTCCTCTTTGCCCACTTCTCG34540.14718691459749875No Hit
CTGAAAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTC34120.14539714898861197No Hit
CTGAAAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG32400.1380676326855518No Hit
CTGAAAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTT32280.13755627108301272No Hit
CTGAAAGGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACAC31040.1322722011901089No Hit
CTGAAAGGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTCACGTCTCGTGCG30350.1293318719755092No Hit
CTGAAAGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG29860.12724381209847463No Hit
CTGAAAGGGGCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGGTCT29660.12639154276090947No Hit
CTGAAAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG28210.12021259006356226No Hit
CTGAAAGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGA27980.11923248032536234No Hit
CTGAAAGGGGCTCTTGAGTCACCCCCGCGCAGCCTAGGCTTGCCGTGCGAGT27730.11816714365340591No Hit
CTGAAAGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT27580.11752794165023207No Hit
CTGAAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC27580.11752794165023207No Hit
CTGAAAGGGGGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACTC27150.11569556257446703No Hit
CTGAAAGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACA27100.11548249524007574No Hit
CTGAAAGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACG25970.11066717348283274No Hit
CTGAAAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT24590.1047865150536333No Hit
CTGAAAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG24160.10295413597786826No Hit
CTGAAAGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAA23830.10154789157088578No Hit
CTGAAAGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAA23700.10099391650146847No Hit
CTGAAAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA23500.10014164716390331No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAA301.8622395E-646.036
TACGCTA253.418253E-546.022
AACGACG206.3128053E-446.034
ATACGCT206.3128053E-446.024
CGATCGT301.8622395E-646.044
TACGTTA206.3128053E-446.044
TGCGTAA206.3128053E-446.044
TGAAAGG2384400.045.115462
CTGAAAG2389450.045.0903781
GAAAGGG2382450.045.0529483
AAAGGGA519400.045.0302244
AAAGGGG1708600.044.8625224
AAGGGGG695700.044.859425
AAGGGGC367000.044.6400575
AGGGGGG265300.044.5088586
AGGGGGC184650.044.505286
AAGGGAT181150.044.5017935
AAGGGAG170800.044.437945
AAGGGGT181500.044.301935
AGGGGGT145700.044.089916
Produced by FastQC (version 0.11.5)