##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527077_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2346676
Sequences flagged as poor quality	0
Sequence length	52
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.32167542515456	33.0	31.0	34.0	31.0	34.0
2	32.740929723574965	34.0	31.0	34.0	31.0	34.0
3	33.017506464462926	34.0	31.0	34.0	31.0	34.0
4	36.06614462328843	37.0	35.0	37.0	35.0	37.0
5	36.265136729569825	37.0	37.0	37.0	35.0	37.0
6	36.425201433857936	37.0	37.0	37.0	35.0	37.0
7	36.45687005790318	37.0	37.0	37.0	35.0	37.0
8	36.577724406777925	37.0	37.0	37.0	35.0	37.0
9	38.71576135776733	39.0	39.0	39.0	39.0	39.0
10	38.20490174186807	39.0	39.0	39.0	37.0	39.0
11	37.85733607877696	39.0	38.0	39.0	35.0	39.0
12	37.28754629953176	39.0	37.0	39.0	35.0	39.0
13	37.16275318791346	39.0	37.0	39.0	34.0	39.0
14	37.881866947120095	40.0	38.0	40.0	33.0	40.0
15	37.88046752086782	40.0	38.0	40.0	33.0	40.0
16	32.8880629452042	40.0	34.0	40.0	3.0	40.0
17	34.55578060200897	40.0	34.0	40.0	17.0	40.0
18	36.618633761115724	40.0	34.0	40.0	31.0	40.0
19	37.507453947626345	40.0	36.0	40.0	33.0	40.0
20	37.73727945400217	40.0	37.0	40.0	33.0	40.0
21	37.91029055566256	40.0	37.0	40.0	34.0	40.0
22	37.968640749724294	40.0	37.0	40.0	34.0	40.0
23	37.96259858625562	40.0	37.0	40.0	34.0	40.0
24	37.90016772660563	40.0	37.0	40.0	34.0	40.0
25	37.87259425672739	40.0	37.0	40.0	34.0	40.0
26	37.75583719269299	40.0	37.0	40.0	34.0	40.0
27	37.67613466878257	40.0	37.0	40.0	34.0	40.0
28	37.56121850651731	40.0	36.0	40.0	33.0	40.0
29	37.375021093666106	40.0	36.0	40.0	33.0	40.0
30	37.37273615957209	40.0	36.0	40.0	33.0	40.0
31	37.311830862036345	40.0	35.0	40.0	33.0	40.0
32	37.239791091739974	40.0	35.0	40.0	33.0	40.0
33	36.88299236878035	39.0	35.0	40.0	32.0	40.0
34	36.75265609739052	39.0	35.0	40.0	31.0	40.0
35	36.66190390151857	39.0	35.0	40.0	31.0	40.0
36	36.599338383313246	39.0	35.0	40.0	31.0	40.0
37	36.31398028530568	39.0	35.0	40.0	31.0	40.0
38	36.250395026837964	38.0	35.0	40.0	31.0	40.0
39	35.8759671126308	38.0	35.0	40.0	30.0	40.0
40	35.83789411064842	38.0	35.0	40.0	30.0	40.0
41	35.71313295913028	38.0	35.0	40.0	30.0	40.0
42	35.65533290492595	38.0	34.0	40.0	30.0	40.0
43	35.58571869316429	38.0	34.0	40.0	30.0	40.0
44	35.832266150077814	38.0	35.0	40.0	30.0	40.0
45	35.89537626838984	38.0	35.0	40.0	30.0	40.0
46	35.84572774426465	37.0	35.0	40.0	31.0	40.0
47	35.50384203017374	37.0	35.0	40.0	30.0	40.0
48	35.435862471001535	37.0	35.0	40.0	30.0	40.0
49	35.32831502942886	36.0	34.0	40.0	30.0	40.0
50	34.83486557155739	36.0	34.0	40.0	28.0	40.0
51	34.89794884338528	36.0	34.0	40.0	28.0	40.0
52	34.5702606580542	35.0	34.0	39.0	28.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	7.0
15	15.0
16	57.0
17	112.0
18	349.0
19	643.0
20	1101.0
21	1888.0
22	3031.0
23	4441.0
24	6376.0
25	9103.0
26	12240.0
27	16624.0
28	22627.0
29	30382.0
30	40407.0
31	54072.0
32	74348.0
33	111838.0
34	164902.0
35	205092.0
36	266608.0
37	446218.0
38	585008.0
39	289186.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	0.0	100.0
2	0.0	0.0	100.0	0.0
3	100.0	0.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	100.0	0.0	0.0
6	0.0	100.0	0.0	0.0
7	99.82260013738582	0.1343602610671435	0.026803870666423483	0.016235730880615817
8	99.81978764857186	0.13636309401042154	0.032556688694988144	0.011292568722738034
9	99.74555498926992	0.17160443111873985	0.03588053911149217	0.04696004049983892
10	71.57374942258753	21.702229025225467	1.855177280544907	4.868844271642101
11	37.56773410560299	25.810039391888782	17.03102601296472	19.59120048954351
12	31.60470384492789	14.138807402470558	28.643792325825977	25.612696426775578
13	25.543023408429626	16.187790730377777	30.1528204149188	28.116365446273793
14	24.027177164636278	17.274817656975227	32.44717208511103	26.25083309327747
15	24.590228902498684	18.192583893132245	30.257862610773707	26.95932459359537
16	28.59350843490963	26.37211102001299	23.190760036749854	21.843620508327525
17	31.83055521938265	18.041093018380042	23.78615539597286	26.342196366264453
18	29.735549347246916	19.047410038710073	24.647629242383694	26.569411371659317
19	30.52100076874694	18.45086411588136	22.964354687225676	28.063780428146025
20	28.960580838598936	20.360501407096677	24.124506322986218	26.55441143131817
21	29.153875524358707	18.52143201703175	26.06503837768827	26.25965408092127
22	29.241190517992262	18.371901361755945	26.051274227886594	26.335633892365202
23	29.354925861090326	19.822293320424293	25.058550903490723	25.764229914994658
24	28.718621573664194	20.570756252673995	23.148615318007256	27.562006855654552
25	28.4432533506969	20.736693092697926	22.631841805174638	28.188211751430536
26	27.876494241216086	20.15438006780655	23.32171122046674	28.64741447051063
27	26.912492393496162	17.415655165007866	24.89171918066235	30.78013326083362
28	27.476652081497406	19.502777545771124	23.299466990756287	29.721103381975183
29	29.608731669817224	20.086283747735095	22.624171381136552	27.680813201311132
30	28.577997132965947	17.97917565100593	24.73451810134846	28.708309114679658
31	30.03682655807619	20.69237508714454	22.048122535876278	27.222675818902992
32	32.129360849132986	19.71921134404579	21.95663142248866	26.194796384332562
33	33.08871782896318	18.676289355667336	22.89306235713835	25.341930458231133
34	29.546516008174965	20.88238853595469	25.81153086322952	23.759564592640825
35	28.44845219365605	23.625161718106803	25.779869057338978	22.14651703089817
36	31.03291634635544	22.89919869636882	22.46705552875642	23.60082942851932
37	29.95436949966676	23.53136947750776	21.437642009378372	25.076619013447104
38	30.90486287838628	23.15228007615879	22.541501255392735	23.401355790062198
39	30.951183716882944	21.315554426772167	22.02046639587229	25.7127954604726
40	29.633703161407883	22.667850184686767	22.284840344384993	25.413606309520357
41	27.38678027985116	21.102614932781517	23.264055199780458	28.246549587586866
42	26.362437763031625	20.377589407314858	23.746354417908567	29.51361841174495
43	26.806086566701154	19.28651420136397	26.83433929524144	27.073059936693433
44	26.58505051400364	18.760919700887555	25.731076637763373	28.92295314734544
45	26.951142807954742	19.739878875481747	25.281675016065275	28.027303300498236
46	27.88088342830455	20.9881977742134	23.736510707059686	27.394408090422367
47	25.842979601785675	20.346140668758704	26.83608644738345	26.974793282072174
48	28.461918049189578	21.546093282583534	25.144118744982265	24.847869923244623
49	29.107128551193263	21.164532300155624	24.20990371061024	25.51843543804087
50	25.32168906146396	21.099461536232525	25.141775004303963	28.437074397999552
51	24.591549919971907	22.961201290676687	25.3480667974616	27.099181991889804
52	26.23246668905294	22.544825105809238	24.685810908706614	26.536897296431206
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	26.0
4	52.0
5	29.5
6	7.0
7	19.5
8	32.0
9	106.0
10	180.0
11	1038.5
12	1897.0
13	2541.5
14	3080.0
15	2974.0
16	3760.5
17	4547.0
18	5136.0
19	5725.0
20	6419.0
21	7113.0
22	6916.0
23	6719.0
24	8034.0
25	9349.0
26	10430.0
27	11511.0
28	12914.5
29	14318.0
30	14306.5
31	14295.0
32	18928.5
33	23562.0
34	27476.0
35	31390.0
36	31796.0
37	32202.0
38	36767.5
39	43890.0
40	46447.0
41	46414.0
42	46381.0
43	52527.0
44	58673.0
45	69414.0
46	80155.0
47	86140.0
48	92125.0
49	93948.0
50	95771.0
51	106687.0
52	117603.0
53	129530.0
54	141457.0
55	172384.0
56	203311.0
57	211994.5
58	220678.0
59	215340.0
60	210002.0
61	205026.0
62	200050.0
63	188848.0
64	157160.0
65	136674.0
66	115815.0
67	94956.0
68	85980.5
69	77005.0
70	66714.0
71	56423.0
72	50155.5
73	43888.0
74	33013.0
75	22138.0
76	15519.0
77	8900.0
78	6308.5
79	3717.0
80	2549.0
81	1381.0
82	1019.0
83	657.0
84	403.0
85	149.0
86	116.5
87	84.0
88	44.0
89	4.5
90	5.0
91	4.5
92	4.0
93	2.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2346676.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	7.897096687485057
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.02848405236458	5.372275160645583
2	10.274335489293279	1.622748415172162
3	4.479506173837963	1.061252801009539
4	2.3666910973891486	0.7475995370196888
5	1.4295425442783136	0.5644617845519615
6	0.8842534275774423	0.4189820888291477
7	0.6297203952577176	0.34810739932020524
8	0.40202556069232115	0.2539867778902122
9	0.3265196283062525	0.23207013675865423
>10	4.599792576136537	10.025769487449617
>50	3.496376547637644	19.932640842560286
>100	2.8834609241227454	40.07517449657506
>500	0.1234605090311195	6.529987580506738
>1k	0.07144415243425191	10.06792616094439
>5k	0.0037602185491711526	2.019197551174716
>10k+	6.267030915285255E-4	0.7278197795920336
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGAAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	14706	0.6266736439116435	No Hit
CTGAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	9429	0.40180237919508277	No Hit
CTGAAAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	7963	0.33933103675155835	No Hit
CTGAAAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	7270	0.3097999042049264	No Hit
CTGAAAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	5913	0.25197342965113206	No Hit
CTGAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	5191	0.2212065065650307	No Hit
CTGAAAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	5033	0.21447357879826615	No Hit
CTGAAAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA	4640	0.19772648631511125	No Hit
CTGAAAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	4198	0.17889133395492177	No Hit
CTGAAAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA	3884	0.16551070535514917	No Hit
CTGAAAGGGGCTTTTTCGCAACGGGTTTGCCGTCAGAACGCAGGTGTTGTGA	3734	0.15911868532341064	No Hit
CTGAAAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC	3717	0.15839425638648028	No Hit
CTGAAAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT	3623	0.15438859049992415	No Hit
CTGAAAGGGGTTACCCTGTCCCTGCCTCTTTCTCCTCTTTGCCCACTTCTCG	3454	0.14718691459749875	No Hit
CTGAAAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTC	3412	0.14539714898861197	No Hit
CTGAAAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	3240	0.1380676326855518	No Hit
CTGAAAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTT	3228	0.13755627108301272	No Hit
CTGAAAGGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACAC	3104	0.1322722011901089	No Hit
CTGAAAGGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTCACGTCTCGTGCG	3035	0.1293318719755092	No Hit
CTGAAAGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG	2986	0.12724381209847463	No Hit
CTGAAAGGGGCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGGTCT	2966	0.12639154276090947	No Hit
CTGAAAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG	2821	0.12021259006356226	No Hit
CTGAAAGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGA	2798	0.11923248032536234	No Hit
CTGAAAGGGGCTCTTGAGTCACCCCCGCGCAGCCTAGGCTTGCCGTGCGAGT	2773	0.11816714365340591	No Hit
CTGAAAGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT	2758	0.11752794165023207	No Hit
CTGAAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	2758	0.11752794165023207	No Hit
CTGAAAGGGGGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACTC	2715	0.11569556257446703	No Hit
CTGAAAGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACA	2710	0.11548249524007574	No Hit
CTGAAAGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACG	2597	0.11066717348283274	No Hit
CTGAAAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT	2459	0.1047865150536333	No Hit
CTGAAAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG	2416	0.10295413597786826	No Hit
CTGAAAGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAA	2383	0.10154789157088578	No Hit
CTGAAAGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAA	2370	0.10099391650146847	No Hit
CTGAAAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA	2350	0.10014164716390331	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.009076668445068685	0.0	0.0	0.0	0.0
7	0.009161895378825198	0.0	0.0	0.0	0.0
8	0.00924712231258171	0.0	0.0	0.0	0.0
9	0.009886324315755562	0.0	0.0	0.0	0.0
10	0.014147671003581237	0.0	0.0	0.0	0.0
11	0.022372070111084785	0.0	0.0	0.0	0.0
12	0.026761257199545228	0.0	0.0	0.0	0.0
13	0.03196010015869255	0.0	0.0	0.0	0.0
14	0.04222994567635242	0.0	0.0	0.0	0.0
15	0.050624798651369	0.0	0.0	0.0	0.0
16	0.06494292352246327	0.0	0.0	0.0	0.0
17	0.07355084383187112	0.0	0.0	0.0	0.0
18	0.07900536759228799	0.0	0.0	0.0	0.0
19	0.08944566697746088	0.0	0.0	0.0	0.0
20	0.09596552740983416	0.0	0.0	0.0	0.0
21	0.10142005117025103	0.0	0.0	0.0	0.0
22	0.10904786174145899	0.0	0.0	0.0	0.0
23	0.11352227576367593	0.0	0.0	0.0	0.0
24	0.1192750937922406	0.0	0.0	0.0	0.0
25	0.12383473474821408	0.0	0.0	0.0	0.0
26	0.12869266997233533	0.0	0.0	0.0	0.0
27	0.13453071493465651	0.0	0.0	0.0	0.0
28	0.13909035589062999	0.0	0.0	0.0	0.0
29	0.144076131515386	0.0	0.0	0.0	0.0
30	0.14906190714014206	0.0	0.0	0.0	0.0
31	0.155027792503098	0.0	0.0	0.0	0.0
32	0.16265560307430596	0.0	0.0	0.0	0.0
33	0.16725785749715769	0.0	0.0	0.0	0.0
34	0.17309590245947887	0.0	0.0	0.0	0.0
35	0.181916890103278	0.0	0.0	0.0	0.0
36	0.19133446628337272	0.0	0.0	0.0	0.0
37	0.19904750378833722	0.0	0.0	0.0	0.0
38	0.20450202754875407	0.0	0.0	0.0	0.0
39	0.21144802264990992	0.0	0.0	0.0	0.0
40	0.21958719482365696	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAA	30	1.8622395E-6	46.0	36
TACGCTA	25	3.418253E-5	46.0	22
AACGACG	20	6.3128053E-4	46.0	34
ATACGCT	20	6.3128053E-4	46.0	24
CGATCGT	30	1.8622395E-6	46.0	44
TACGTTA	20	6.3128053E-4	46.0	44
TGCGTAA	20	6.3128053E-4	46.0	44
TGAAAGG	238440	0.0	45.11546	2
CTGAAAG	238945	0.0	45.090378	1
GAAAGGG	238245	0.0	45.052948	3
AAAGGGA	51940	0.0	45.030224	4
AAAGGGG	170860	0.0	44.862522	4
AAGGGGG	69570	0.0	44.85942	5
AAGGGGC	36700	0.0	44.640057	5
AGGGGGG	26530	0.0	44.508858	6
AGGGGGC	18465	0.0	44.50528	6
AAGGGAT	18115	0.0	44.501793	5
AAGGGAG	17080	0.0	44.43794	5
AAGGGGT	18150	0.0	44.30193	5
AGGGGGT	14570	0.0	44.08991	6
>>END_MODULE