FastQCFastQC Report
Fri 17 Jun 2016
SRR1527076_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527076_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2277617
Sequences flagged as poor quality0
Sequence length52
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGAAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA143140.6284638725474915No Hit
CTGAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC87780.3854028135546933No Hit
CTGAAAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT83020.3645037774129716No Hit
CTGAAAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG69420.30479224557948065No Hit
CTGAAAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG58740.25790113087494515No Hit
CTGAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT51250.2250158828284123No Hit
CTGAAAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC47000.20635602913044643No Hit
CTGAAAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG41380.18168111671101858No Hit
CTGAAAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA40940.1797492730340527No Hit
CTGAAAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA39070.1715389374069477No Hit
CTGAAAGGGGCTTTTTCGCAACGGGTTTGCCGTCAGAACGCAGGTGTTGTGA37200.1633286017798427No Hit
CTGAAAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT34400.15103505110824164No Hit
CTGAAAGGGGTTACCCTGTCCCTGCCTCTTTCTCCTCTTTGCCCACTTCTCG33340.1463810640682784No Hit
CTGAAAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTC33230.14589810314903692No Hit
CTGAAAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTT32610.14317595978603953No Hit
CTGAAAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC31980.1404099108849293No Hit
CTGAAAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG31870.13992694996568783No Hit
CTGAAAGGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTCACGTCTCGTGCG29790.13079459803821275No Hit
CTGAAAGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGA29020.12741387160352247No Hit
CTGAAAGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG28200.12381361747826784No Hit
CTGAAAGGGGCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGGTCT28150.12359408978770356No Hit
CTGAAAGGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACAC28000.12293550671601064No Hit
CTGAAAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG27660.12144271842017336No Hit
CTGAAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC27270.1197304024337718No Hit
CTGAAAGGGGCTCTTGAGTCACCCCCGCGCAGCCTAGGCTTGCCGTGCGAGT26030.11428611570777703No Hit
CTGAAAGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACA26020.11424221016966417No Hit
CTGAAAGGGGGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACTC25320.11116882250176391No Hit
CTGAAAGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACG24980.10967603420592663No Hit
CTGAAAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT24530.1077002849908479No Hit
CTGAAAGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAA24380.10704170191915498No Hit
CTGAAAGGGGACTGTCGAGTCGCGTCCACCCGCGAGCACAGCTTCTTTGCAG24090.10576844131388202No Hit
CTGAAAGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT23980.10528548039464053No Hit
CTGAAAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA23310.10234380934107887No Hit
CTGAAAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG22990.10093883212146731No Hit
CTGAAAGGGGACTACCCAGATGCTTACACCACATGAAACACTGTCTCTTCTA22810.10014853243543581No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGAA351.020162E-746.00000424
TCGACTA351.020162E-746.00000440
TATCGAC351.020162E-746.00000444
CGTTACA206.3127663E-446.015
TAACACG301.862214E-646.033
CCGCATA405.6152203E-946.019
GCGTAAT206.3127663E-446.021
TGAAAGG2315700.045.1070942
AAAGGGA505600.045.090194
CTGAAAG2320800.045.075361
GAAAGGG2311750.045.069753
AAGGGGG676000.044.938465
AAAGGGG1658450.044.9127244
AGGGGGC183100.044.7312936
AGGGGGG256700.044.5843436
AAGGGGT176800.044.5820125
AAGGGAT177300.044.5341225
AAGGGGC357800.044.4058155
AAGGGAG166300.044.146725
AAGGGGA462250.043.9948045
Produced by FastQC (version 0.11.5)