FastQCFastQC Report
Fri 17 Jun 2016
SRR1527072_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527072_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5678250
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC1127321.9853299872319818No Hit
GAATTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG540870.9525293884559503No Hit
GAATTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC472220.8316294633029542No Hit
GAATTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT210320.3703958085677806No Hit
GAATTAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT159780.28138951261392153No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGC125710.2213886320609343No Hit
GAATTAGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGA119520.21048738607845727No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGGGGGTAAGAGCACCCGACTAC118010.20782811605688373No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT116210.20465812530269012No Hit
GAATTAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCT94980.16726984546295073No Hit
GAATTAGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCG88310.15552326861268875No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAGGAGCACCCGACTGC80420.14162814247347333No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGC79560.1401135913353586No Hit
GAATTAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT76000.13384405406595343No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC72740.12810284858891385No Hit
GAATTAGGGGGGGCTGGAGAGATGGCTTAGCGGTTAAAAGCACTGACTGC69650.1226610311275481No Hit
GAATTAGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCA67300.11852243208735086No Hit
GAATTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACT62530.11012195658873772No Hit
GAATTAGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTG60560.10665257781887025No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATTAG6018000.041.3912931
ATTAGGG5925000.041.3878483
AATTAGG5991850.041.3835752
TAGGGAT411150.041.351335
TTAGGGG3526950.041.3277784
TAGGGGG1546350.041.2570275
TTAGGGA1483600.041.2047734
TTAGGGC590550.041.176194
TAGGGGC750500.041.15075
TAGGGGA723500.041.147755
TAGGGAG508250.041.056575
TAGGGCA274550.040.826815
TAGGGGT522450.040.787065
TAGGGTG149350.040.685645
TAGGGAA298600.040.5740135
AGGGGGG965950.040.570016
TTAGGGT345800.040.5263144
AGGGATA49000.040.4979636
AGGGATC121050.040.2924426
AGGGATT148000.040.1648646
Produced by FastQC (version 0.11.5)