##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527072_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5678250
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.327078237132916	33.0	31.0	34.0	31.0	34.0
2	31.606352661471405	31.0	31.0	34.0	30.0	34.0
3	32.52903236032228	34.0	31.0	34.0	31.0	34.0
4	36.38270364989213	37.0	37.0	37.0	35.0	37.0
5	36.48472821732048	37.0	37.0	37.0	35.0	37.0
6	36.256277021969794	37.0	37.0	37.0	35.0	37.0
7	36.4539997358341	37.0	37.0	37.0	35.0	37.0
8	36.636280544181744	37.0	37.0	37.0	35.0	37.0
9	38.75434790648527	39.0	39.0	39.0	39.0	39.0
10	38.26802183771409	39.0	39.0	39.0	37.0	39.0
11	37.96763914938582	39.0	38.0	39.0	35.0	39.0
12	37.277306212301326	39.0	35.0	39.0	35.0	39.0
13	37.12804015321622	39.0	35.0	39.0	35.0	39.0
14	37.6791830229384	40.0	36.0	40.0	33.0	40.0
15	37.72700250957601	40.0	36.0	40.0	33.0	40.0
16	37.64188156562321	40.0	36.0	40.0	33.0	40.0
17	37.538972218553255	40.0	36.0	40.0	33.0	40.0
18	37.58640426187646	40.0	36.0	40.0	33.0	40.0
19	37.57638973275217	40.0	36.0	40.0	33.0	40.0
20	37.34013560516004	40.0	36.0	40.0	33.0	40.0
21	37.54701413287545	40.0	36.0	40.0	33.0	40.0
22	37.43718011711355	40.0	36.0	40.0	33.0	40.0
23	37.52942984194074	40.0	35.0	40.0	34.0	40.0
24	37.62102144146524	40.0	36.0	40.0	34.0	40.0
25	37.581721304979524	40.0	35.0	40.0	34.0	40.0
26	37.461231541408004	40.0	35.0	40.0	34.0	40.0
27	37.32083405978955	39.0	35.0	40.0	34.0	40.0
28	37.172226478228325	39.0	35.0	40.0	33.0	40.0
29	37.37893136089464	40.0	35.0	40.0	34.0	40.0
30	37.40488865407476	40.0	35.0	40.0	34.0	40.0
31	37.367863514286974	40.0	35.0	40.0	34.0	40.0
32	36.67420613745432	40.0	35.0	40.0	32.0	40.0
33	36.87687438911637	39.0	35.0	40.0	32.0	40.0
34	36.653937921014396	39.0	35.0	40.0	31.0	40.0
35	36.71726852463347	39.0	35.0	40.0	31.0	40.0
36	36.6231904195835	39.0	35.0	40.0	31.0	40.0
37	36.61593202130938	39.0	35.0	40.0	31.0	40.0
38	36.41453053317483	39.0	35.0	40.0	31.0	40.0
39	36.379927090212654	39.0	35.0	40.0	31.0	40.0
40	36.260086998635146	39.0	35.0	40.0	31.0	40.0
41	36.08623625236648	39.0	35.0	40.0	31.0	40.0
42	36.012628186501125	39.0	35.0	40.0	30.0	40.0
43	35.93617382115969	38.0	35.0	40.0	30.0	40.0
44	35.76565684850086	38.0	35.0	40.0	30.0	40.0
45	35.661055606921146	38.0	35.0	40.0	29.0	40.0
46	35.39960005283318	38.0	35.0	40.0	29.0	40.0
47	35.28723796944481	38.0	35.0	40.0	28.0	40.0
48	35.203112578699425	37.0	35.0	40.0	28.0	40.0
49	34.96890186236957	37.0	34.0	40.0	27.0	40.0
50	34.59976559679479	36.0	34.0	40.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	4.0
12	4.0
13	6.0
14	15.0
15	45.0
16	129.0
17	349.0
18	804.0
19	1622.0
20	2738.0
21	4508.0
22	7015.0
23	10857.0
24	15682.0
25	21704.0
26	29310.0
27	39325.0
28	53716.0
29	75498.0
30	104781.0
31	138284.0
32	164383.0
33	218507.0
34	480015.0
35	501338.0
36	429236.0
37	734006.0
38	1737012.0
39	907356.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	100.0	0.0	0.0
4	0.0	0.0	100.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	100.0	0.0	0.0
7	99.69097873464536	0.27196759565006823	0.026592700215735482	0.01046096948883899
8	99.52474794170739	0.4347290098181658	0.025219037555584908	0.015304010918857042
9	98.80889358517149	0.6651521155285519	0.1817285255140228	0.3442257737859376
10	59.51833751596002	24.65949896535024	5.746118962708581	10.076044555981156
11	38.169436005811654	22.824285651389072	19.317589045920837	19.68868929687844
12	35.663082816008455	14.833126403381325	26.289719543873552	23.21407123673667
13	26.872082067538415	15.050552546999516	28.25560692114648	29.82175846431559
14	23.183480825958704	16.994461321710034	34.433284902919034	25.388772949412232
15	28.055721393034826	17.955919517456962	30.412257297582883	23.576101791925332
16	33.48225245454145	19.12605116012856	26.584986571566944	20.806709813763042
17	31.45183815436094	19.60855897503632	26.17415576982345	22.76544710077929
18	31.009289834015764	20.450138687095496	26.02497248271915	22.515598996169594
19	29.122934002553603	24.115757495707303	24.693646810196803	22.06766169154229
20	30.06457975608682	24.00449082023511	24.676810637080084	21.254118786597985
21	27.625447981332275	25.52113767445956	26.55466032668516	20.298754017523006
22	26.579932197419982	22.034183066966055	30.06966935235328	21.316215383260687
23	29.40976533262889	20.831946462378372	28.406304759388895	21.35198344560384
24	31.771144278606968	20.459507770880112	25.749922951613613	22.01942499889931
25	28.08974596046317	20.167762955135828	24.363509884207282	27.37898120019372
26	24.837599612556684	19.658979439087748	28.753189803196406	26.75023114515916
27	23.022885572139305	18.912252894817946	28.41072513538502	29.654136397657727
28	24.771910359705895	23.36726984546295	25.443208735085634	26.41761105974552
29	29.66442125654911	23.377255316338662	22.973222383656935	23.98510104345529
30	28.906406022982434	19.841570906529302	27.352353277858494	23.899669792629773
31	31.72563730022454	19.637705278915156	26.159749922951615	22.476907497908687
32	34.35861400959803	19.531774754545854	24.59805397789812	21.511557257957996
33	34.29347069959935	19.45639941883503	25.17680623431515	21.073323647250472
34	27.22012944128913	20.602315854356537	32.129370844890595	20.048183859463744
35	25.441993571963195	27.02971866332057	28.064368423369878	19.463919341346365
36	25.472636815920396	28.780768722757895	25.144349051204156	20.602245410117554
37	29.820384801655443	26.992083828644386	22.82661031127548	20.36092105842469
38	27.97788050895963	27.992004578875534	22.7510588649672	21.27905604719764
39	30.587593008409282	23.965341434420818	22.519103597058955	22.92796196011095
40	27.76503324087527	23.434209483555673	22.041491656760446	26.759265618808612
41	23.461066349667593	26.256064808699865	23.397455201866773	26.88541363976577
42	22.251292211508826	23.129837537973845	24.50115792717827	30.117712323339056
43	23.021300576762208	21.284427420420023	25.709030070884513	29.985241931933253
44	24.6657508915599	20.687870382600273	24.728164487298024	29.9182142385418
45	27.870910932065335	21.76476907497909	23.849390217056314	26.514929775899265
46	25.8669132215031	26.12996962092194	22.982732355919516	25.02038480165544
47	23.74002553603663	22.94432263461454	25.72709901818342	27.588552811165414
48	25.191617135561135	22.06415709065293	28.311046537225376	24.43317923656056
49	28.640496631884826	21.878888742130055	25.05167965482323	24.42893497116189
50	23.976084180865584	21.827129837537974	25.885105446220223	28.311680535376215
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	1.0
2	2.0
3	9.5
4	17.0
5	46.5
6	76.0
7	1075.0
8	2074.0
9	6536.0
10	10998.0
11	15402.5
12	19807.0
13	19568.5
14	19330.0
15	22482.5
16	25635.0
17	27995.5
18	30356.0
19	28203.0
20	26050.0
21	30120.5
22	34191.0
23	36429.5
24	38668.0
25	44735.5
26	50803.0
27	54854.0
28	58905.0
29	68442.5
30	77980.0
31	82269.5
32	86559.0
33	92617.5
34	98676.0
35	106887.0
36	115098.0
37	124136.0
38	133174.0
39	146469.5
40	159765.0
41	178060.0
42	196355.0
43	202818.5
44	209282.0
45	227061.0
46	244840.0
47	270962.5
48	297085.0
49	314478.0
50	331871.0
51	361100.0
52	390329.0
53	445756.0
54	501183.0
55	522260.5
56	543338.0
57	539784.5
58	536231.0
59	548699.0
60	561167.0
61	439482.5
62	317798.0
63	251551.0
64	185304.0
65	157086.5
66	128869.0
67	114034.5
68	99200.0
69	80755.5
70	62311.0
71	52351.5
72	42392.0
73	34592.0
74	26792.0
75	17762.5
76	8733.0
77	6484.5
78	4236.0
79	3041.0
80	1846.0
81	1196.5
82	547.0
83	431.5
84	316.0
85	177.5
86	39.0
87	27.5
88	16.0
89	11.0
90	6.0
91	3.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	5678250.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	6.610605661730568
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.9227318651157	4.225679731808188
2	10.9027186739877	1.4414714758903733
3	5.012011996742952	0.9939730464699146
4	2.7484209037294587	0.726749071480504
5	1.6803717202035309	0.5554137403694697
6	1.0283161579286497	0.4078675569391294
7	0.7162551760518915	0.33144163654367204
8	0.5385554481562156	0.28481421557898573
9	0.4054702092004709	0.24123632945433396
>10	5.873942863128885	10.385603966693147
>50	3.6874990233754783	17.608081900933296
>100	3.2474701787151004	38.5494634177374
>500	0.14887629648259704	6.6593261933782495
>1k	0.08089881771129802	10.24997804053564
>5k	0.003932581416521432	1.786811989598638
>10k+	0.002528088053478063	5.552087686589082
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	112732	1.9853299872319818	No Hit
GAATTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	54087	0.9525293884559503	No Hit
GAATTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	47222	0.8316294633029542	No Hit
GAATTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	21032	0.3703958085677806	No Hit
GAATTAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	15978	0.28138951261392153	No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGC	12571	0.2213886320609343	No Hit
GAATTAGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGA	11952	0.21048738607845727	No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGGGGGTAAGAGCACCCGACTAC	11801	0.20782811605688373	No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT	11621	0.20465812530269012	No Hit
GAATTAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCT	9498	0.16726984546295073	No Hit
GAATTAGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCG	8831	0.15552326861268875	No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAGGAGCACCCGACTGC	8042	0.14162814247347333	No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGC	7956	0.1401135913353586	No Hit
GAATTAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	7600	0.13384405406595343	No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC	7274	0.12810284858891385	No Hit
GAATTAGGGGGGGCTGGAGAGATGGCTTAGCGGTTAAAAGCACTGACTGC	6965	0.1226610311275481	No Hit
GAATTAGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCA	6730	0.11852243208735086	No Hit
GAATTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACT	6253	0.11012195658873772	No Hit
GAATTAGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	6056	0.10665257781887025	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0075903667503192	0.0	0.0	0.0	0.0
7	0.007696033108792321	0.0	0.0	0.0	0.0
8	0.007731255228283362	0.0	0.0	0.0	0.0
9	0.00836525337912209	0.0	0.0	0.0	0.0
10	0.015039845022674239	0.0	0.0	0.0	0.0
11	0.027948751816140538	0.0	0.0	0.0	0.0
12	0.033584290934706995	0.0	0.0	0.0	0.0
13	0.04166776735790076	0.0	0.0	0.0	0.0
14	0.057852331264033816	0.0	0.0	0.0	0.0
15	0.06966935235327786	0.0	0.0	0.0	0.0
16	0.0873332452780346	0.0	0.0	0.0	0.0
17	0.09647338528595958	0.0	0.0	0.0	0.0
18	0.10541980363668384	0.0	0.0	0.0	0.0
19	0.12112886892968784	0.0	0.0	0.0	0.0
20	0.13109672874565226	0.0	0.0	0.0	0.0
21	0.14127592127856295	0.0	0.0	0.0	0.0
22	0.15115572579579975	0.0	0.0	0.0	0.0
23	0.15969708977237707	0.0	0.0	0.0	0.0
24	0.1680271210320081	0.0	0.0	0.0	0.0
25	0.17556465460309073	0.0	0.0	0.0	0.0
26	0.18252102320257121	0.0	0.0	0.0	0.0
27	0.19005855677365385	0.0	0.0	0.0	0.0
28	0.1982829216748118	0.0	0.0	0.0	0.0
29	0.20375996125566856	0.0	0.0	0.0	0.0
30	0.2095539999119447	1.7611059745520188E-5	0.0	0.0	0.0
31	0.215048650552547	1.7611059745520188E-5	0.0	0.0	0.0
32	0.2218641306740633	1.7611059745520188E-5	0.0	0.0	0.0
33	0.2269889490600097	1.7611059745520188E-5	0.0	0.0	0.0
34	0.23387487342050808	1.7611059745520188E-5	0.0	0.0	0.0
35	0.2459208382864439	1.7611059745520188E-5	0.0	0.0	0.0
36	0.25941091005151234	1.7611059745520188E-5	0.0	0.0	0.0
37	0.2679346629683441	1.7611059745520188E-5	0.0	0.0	0.0
38	0.27527847488222607	1.7611059745520188E-5	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAATTAG	601800	0.0	41.391293	1
ATTAGGG	592500	0.0	41.387848	3
AATTAGG	599185	0.0	41.383575	2
TAGGGAT	41115	0.0	41.35133	5
TTAGGGG	352695	0.0	41.327778	4
TAGGGGG	154635	0.0	41.257027	5
TTAGGGA	148360	0.0	41.204773	4
TTAGGGC	59055	0.0	41.17619	4
TAGGGGC	75050	0.0	41.1507	5
TAGGGGA	72350	0.0	41.14775	5
TAGGGAG	50825	0.0	41.05657	5
TAGGGCA	27455	0.0	40.82681	5
TAGGGGT	52245	0.0	40.78706	5
TAGGGTG	14935	0.0	40.68564	5
TAGGGAA	29860	0.0	40.574013	5
AGGGGGG	96595	0.0	40.57001	6
TTAGGGT	34580	0.0	40.526314	4
AGGGATA	4900	0.0	40.497963	6
AGGGATC	12105	0.0	40.292442	6
AGGGATT	14800	0.0	40.164864	6
>>END_MODULE