FastQCFastQC Report
Fri 17 Jun 2016
SRR1527071_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527071_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6065247
Sequences flagged as poor quality0
Sequence length52
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC1059541.74690330006346No Hit
GAATTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT480610.7923997159555085No Hit
GAATTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA478260.7885251828985695No Hit
GAATTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC191420.31560132670606816No Hit
GAATTAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT148370.2446231785778881No Hit
GAATTAGGGGGGCTGATGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC125970.20769145922663992No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGGGGGTAAGAGCACCCGACTACTC117200.19323203160563782No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGCTC116730.19245712499425002No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC115570.19054458952784611No Hit
GAATTAGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACC111560.18393315226898427No Hit
GAATTAGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC88840.14647383692700397No Hit
GAATTAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT77260.12738145701238549No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAGGAGCACCCGACTGCTC74610.12301230271413513No Hit
GAATTAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG72720.11989618889387357No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTC71660.11814852717457343No Hit
GAATTAGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG68990.11374639812690233No Hit
GAATTAGGGGGGGCTGGAGAGATGGCTTAGCGGTTAAAAGCACTGACTGCTC65340.10772850635761412No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC64720.10670628912557065No Hit
GAATTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGC61910.10207333683195424No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATTAG6436750.043.2171671
AATTAGG6408850.043.1982352
ATTAGGG6340100.043.1943363
TAGGGGG1652950.043.0723885
TTAGGGG3800350.043.0659524
TAGGGAG548500.042.9766655
TTAGGGC620900.042.8735734
TTAGGGA1593050.042.8323674
TAGGGGC816100.042.702615
TAGGGGA789950.042.599285
TAGGGGT563450.042.5384675
TTAGGGT361250.042.45374
TAGGGAT445100.042.3259935
TAGGGAA323750.042.3128975
TAGGGCA292600.042.297685
AGGGGGG1054150.042.2799386
TAGGGTG158900.042.222155
AGGGATC127250.042.07786
TAGGGAC291350.041.6107755
TAGGGCC77300.041.4476055